##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937239_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1959546 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.136032529983986 33.0 31.0 34.0 31.0 34.0 2 32.317765951909266 34.0 31.0 34.0 31.0 34.0 3 32.39770844879375 34.0 31.0 34.0 31.0 34.0 4 36.047844245554835 37.0 35.0 37.0 35.0 37.0 5 35.975519839799624 37.0 35.0 37.0 35.0 37.0 6 36.01463451227988 37.0 35.0 37.0 35.0 37.0 7 35.95151376900568 37.0 35.0 37.0 35.0 37.0 8 35.93243077733312 37.0 35.0 37.0 35.0 37.0 9 37.612497486662726 39.0 37.0 39.0 35.0 39.0 10-11 37.52776459445198 39.0 37.0 39.0 35.0 39.0 12-13 37.424305936170924 39.0 37.0 39.0 34.0 39.0 14-15 38.618686675382975 40.0 38.0 41.0 34.0 41.0 16-17 38.57773739427398 40.0 38.0 41.0 34.0 41.0 18-19 38.56886314483049 40.0 38.0 41.0 34.0 41.0 20-21 38.46283169672975 40.0 38.0 41.0 34.0 41.0 22-23 38.41400890818588 40.0 38.0 41.0 34.0 41.0 24-25 38.45023847360562 40.0 38.0 41.0 34.0 41.0 26-27 38.29194338892785 40.0 38.0 41.0 34.0 41.0 28-29 38.14904217609589 40.0 38.0 41.0 33.5 41.0 30-31 38.204166424263576 40.0 38.0 41.0 34.0 41.0 32-33 38.11686482481146 40.0 37.0 41.0 33.5 41.0 34-35 37.98250717257977 40.0 37.0 41.0 33.0 41.0 36-37 37.8209822581353 40.0 37.0 41.0 33.0 41.0 38-39 37.71316468202328 40.0 37.0 41.0 33.0 41.0 40-41 37.54081557666929 40.0 37.0 41.0 33.0 41.0 42-43 37.34056562081217 40.0 37.0 41.0 32.5 41.0 44-45 37.049707942554036 39.5 36.0 41.0 31.5 41.0 46-47 36.7141396527563 39.0 36.0 41.0 31.0 41.0 48-49 36.39352099925187 39.0 36.0 41.0 30.0 41.0 50-51 36.14472587017605 39.0 35.0 40.0 30.0 41.0 52-53 35.922916328578154 39.0 35.0 40.0 29.0 41.0 54-55 35.71218996645141 39.0 35.0 40.0 28.5 41.0 56-57 35.40835632335245 38.0 35.0 40.0 28.0 41.0 58-59 35.14250928531405 38.0 34.0 40.0 28.0 41.0 60-61 34.77907076435052 38.0 34.0 40.0 26.5 41.0 62-63 34.413431478515946 37.0 34.0 40.0 26.0 41.0 64-65 34.00036641140346 37.0 33.0 39.5 26.0 41.0 66-67 33.546870805788686 36.0 33.0 39.0 25.0 40.0 68-69 33.094963578298234 36.0 32.0 39.0 24.0 40.0 70-71 32.639199590109136 35.0 32.0 38.0 22.5 40.0 72-73 32.152277109085475 35.0 31.0 37.5 22.0 39.5 74-75 31.664270193197815 35.0 31.0 37.0 20.5 39.0 76-77 30.335835443515997 33.5 29.5 35.5 20.0 38.0 78-79 31.00457554964262 34.0 31.0 36.0 20.0 38.0 80-81 30.802252409486687 34.0 31.0 36.0 19.5 37.0 82-83 30.395404343659195 34.0 30.0 35.0 18.0 37.0 84-85 29.928364019012566 34.0 30.0 35.0 14.0 36.5 86-87 29.546948629937752 34.0 29.0 35.0 7.5 36.0 88-89 29.069633476325638 34.0 29.0 35.0 4.5 35.5 90-91 28.476715269761463 33.0 29.0 35.0 2.0 35.0 92-93 27.96583239178871 33.0 27.0 35.0 2.0 35.0 94-95 27.420870446521796 33.0 26.5 34.5 2.0 35.0 96-97 26.6886827356949 32.0 25.5 34.0 2.0 35.0 98-99 25.652825450384938 32.0 24.0 34.0 2.0 35.0 100-101 22.598122218105622 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2894737377944594 1101 2 0.28968012550090805 1101 3 0.25996495509011197 1101 4 0.22507183958238386 1101 5 0.11680664529378504 1101 6 0.2283159696446262 1101 7 0.23388102974351455 1101 8 0.19165648151644632 1101 9 0.31786641056888953 1101 10-11 0.2442167109135127 1101 12-13 0.2607927288162273 1101 14-15 0.3732441358135503 1101 16-17 0.35292976982355384 1101 18-19 0.42486486155937087 1101 20-21 0.3742683607850452 1101 22-23 0.42533095005350674 1101 24-25 0.42858381574446724 1101 26-27 0.30742860723987775 1101 28-29 0.26693989116161276 1101 30-31 0.35093385891747175 1101 32-33 0.3537013986296884 1101 34-35 0.4537638094480414 1101 36-37 0.383158927030685 1101 38-39 0.36200127328721265 1101 40-41 0.3715708015989634 1101 42-43 0.23231946797137226 1101 44-45 0.2087551607849818 1101 46-47 0.20859286796464716 1101 48-49 0.24264850619857725 1101 50-51 0.17564462411320392 1101 52-53 0.13815654299834534 1101 54-55 0.12081349482824066 1101 56-57 0.1651436505037438 1101 58-59 0.372103043979763 1101 60-61 0.40935406961893506 1101 62-63 0.5001121491323488 1101 64-65 0.37371563809212915 1101 66-67 0.3059804964206734 1101 68-69 0.31574128813476676 1101 70-71 0.43013759199899937 1101 72-73 0.4044390596178715 1101 74-75 0.34785189284938767 1101 76-77 0.4843655573189771 1101 78-79 0.5785431821008373 1101 80-81 0.5024551869894118 1101 82-83 0.337119212174148 1101 84-85 0.4712301901178435 1101 86-87 0.6945925645976807 1101 88-89 0.8830770022050309 1101 90-91 0.9583261757755572 1101 92-93 0.94224574253181 1101 94-95 1.1137902736863374 1101 96-97 1.4751996620565677 1101 98-99 1.7380070436903203 1101 100-101 2.737234106456352 1102 1 0.30531995629403497 1102 2 0.2768589624304951 1102 3 0.29282273280347937 1102 4 0.12320173944761592 1102 5 0.1375436719722316 1102 6 0.10192661783973023 1102 7 0.1328743685835363 1102 8 0.10723630192114086 1102 9 0.1925868495581753 1102 10-11 0.23752596456410657 1102 12-13 0.2765030915330513 1102 14-15 0.14994975988066983 1102 16-17 0.35070898062826217 1102 18-19 0.3467236343408757 1102 20-21 0.2831827945864447 1102 22-23 0.3152370465364669 1102 24-25 0.1609648302309168 1102 26-27 0.2572736712671997 1102 28-29 0.2948790857360777 1102 30-31 0.3536582202141503 1102 32-33 0.31963057972725295 1102 34-35 0.28826006828169426 1102 36-37 0.2528152662426493 1102 38-39 0.23051521631715133 1102 40-41 0.211063534220294 1102 42-43 0.2659434722994334 1102 44-45 0.3466656867702653 1102 46-47 0.43262931837305274 1102 48-49 0.5872439820198707 1102 50-51 0.5382231789458061 1102 52-53 0.4354615265507391 1102 54-55 0.5107002695998872 1102 56-57 0.49861344342330227 1102 58-59 0.5946348161188197 1102 60-61 0.6412109826857275 1102 62-63 0.6422118636540688 1102 64-65 0.7356783133762761 1102 66-67 0.5915596164133277 1102 68-69 0.6948791533754886 1102 70-71 0.6677209772594352 1102 72-73 0.8477467433794246 1102 74-75 0.6769169393905621 1102 76-77 0.8669155160188211 1102 78-79 0.8366250916655673 1102 80-81 0.9600168594767418 1102 82-83 0.978631425910919 1102 84-85 1.0281495364643511 1102 86-87 1.1007971283858922 1102 88-89 1.1464089830185706 1102 90-91 1.2761973096218817 1102 92-93 1.2946508125488592 1102 94-95 1.3523783879356124 1102 96-97 1.5714712963352149 1102 98-99 1.9830461943078888 1102 100-101 2.580602653608576 1103 1 0.3349417767024647 1103 2 0.35556649649982575 1103 3 0.2953227297661911 1103 4 0.19316915535404888 1103 5 0.2060103607504047 1103 6 0.1654309751421792 1103 7 0.21409344133402186 1103 8 0.15224870015659064 1103 9 0.21621973300747044 1103 10-11 0.3163183910099505 1103 12-13 0.30209153693647295 1103 14-15 0.24799635440552237 1103 16-17 0.346213177561566 1103 18-19 0.29274423205610134 1103 20-21 0.4718186667333626 1103 22-23 0.32725060866310685 1103 24-25 0.4190495002774881 1103 26-27 0.3085916443956549 1103 28-29 0.4026334803972418 1103 30-31 0.2990042348587707 1103 32-33 0.23418435259352322 1103 34-35 0.33977713605344917 1103 36-37 0.23168982112716918 1103 38-39 0.33468013653957485 1103 40-41 0.34904978985598945 1103 42-43 0.6106798715991602 1103 44-45 0.4476218573981683 1103 46-47 0.4533814800154303 1103 48-49 0.3222604016330166 1103 50-51 0.2594508897613679 1103 52-53 0.4038893075885497 1103 54-55 0.2998250246421037 1103 56-57 0.24679334479628068 1103 58-59 0.25909063624296635 1103 60-61 0.4259463918275017 1103 62-63 0.4279062984804156 1103 64-65 0.5288525410834524 1103 66-67 0.5066430934106876 1103 68-69 0.8792035862215215 1103 70-71 0.696253168327118 1103 72-73 0.6443265373536704 1103 74-75 0.6519391420121075 1103 76-77 0.5693437417165192 1103 78-79 0.6123760323156198 1103 80-81 0.6295059318992031 1103 82-83 0.610118023545958 1103 84-85 0.5696931927106696 1103 86-87 0.7656564530617196 1103 88-89 0.9252389807952746 1103 90-91 1.3033938505081153 1103 92-93 1.2709172427925388 1103 94-95 1.2348186959901533 1103 96-97 1.3958754399336328 1103 98-99 1.907142994093089 1103 100-101 2.8001132300748885 1104 1 0.35057381942868915 1104 2 0.3270797129823748 1104 3 0.23708321845469982 1104 4 0.18280438845423674 1104 5 0.1993538264976209 1104 6 0.1874674566918273 1104 7 0.24049026262326834 1104 8 0.2032627919873633 1104 9 0.2643307789179943 1104 10-11 0.24249285198132497 1104 12-13 0.2696046367869158 1104 14-15 0.32930958328022797 1104 16-17 0.28227630954658167 1104 18-19 0.37486932970511333 1104 20-21 0.3698273861979189 1104 22-23 0.3072348390309543 1104 24-25 0.2672010333214203 1104 26-27 0.38944806420318656 1104 28-29 0.3726936552724993 1104 30-31 0.3864948648057691 1104 32-33 0.435524619462349 1104 34-35 0.4041541080167903 1104 36-37 0.5958064362205775 1104 38-39 0.42863883662619884 1104 40-41 0.5573659624763607 1104 42-43 0.5645085937122403 1104 44-45 0.6786193291543654 1104 46-47 0.6914593404481124 1104 48-49 0.7194183749558576 1104 50-51 0.6210046358994532 1104 52-53 0.553011195424908 1104 54-55 0.6061748832864282 1104 56-57 0.7268143264255045 1104 58-59 0.644579628480848 1104 60-61 0.7780763248490743 1104 62-63 0.8406114221529606 1104 64-65 0.5966054656941608 1104 66-67 0.769263810673813 1104 68-69 0.8044266147441448 1104 70-71 0.8462529860894534 1104 72-73 0.8974156175516086 1104 74-75 0.8830427672051222 1104 76-77 0.9852929994625264 1104 78-79 0.8534573212461432 1104 80-81 0.9420808771367852 1104 82-83 0.803962551738735 1104 84-85 0.8581716115195377 1104 86-87 0.9275079451629331 1104 88-89 1.1734509256234276 1104 90-91 1.2839235789375536 1104 92-93 1.3280393335201666 1104 94-95 1.3841112797678434 1104 96-97 1.6203123559378625 1104 98-99 1.9151653996059004 1104 100-101 2.312390733078775 1105 1 0.22747203328866306 1105 2 0.24996587512223556 1105 3 0.17994502020908953 1105 4 0.17319951588302018 1105 5 0.19349226315876678 1105 6 0.23570552881191276 1105 7 0.16651998921129518 1105 8 0.18057841833462618 1105 9 0.304671490779846 1105 10-11 0.21188008081453802 1105 12-13 0.2546950185111925 1105 14-15 0.32831947937799555 1105 16-17 0.41469575783079193 1105 18-19 0.5696687209425377 1105 20-21 0.49344754083448805 1105 22-23 0.47632086750986957 1105 24-25 0.3712618490484516 1105 26-27 0.4650387603147905 1105 28-29 0.4837718246858884 1105 30-31 0.49357509595765237 1105 32-33 0.4957799773265421 1105 34-35 0.6105697712999287 1105 36-37 0.5487999718241667 1105 38-39 0.5017304931941808 1105 40-41 0.3802828221607868 1105 42-43 0.45176877300566787 1105 44-45 0.5471767051504983 1105 46-47 0.7885143835543786 1105 48-49 0.6266260904779344 1105 50-51 0.7436771505410036 1105 52-53 0.8455530818229917 1105 54-55 0.7190391618447052 1105 56-57 0.889092232470027 1105 58-59 0.6888815280226694 1105 60-61 0.9085186536129868 1105 62-63 1.0137653385296375 1105 64-65 0.9681976985359668 1105 66-67 0.9015577202068599 1105 68-69 0.9150316590239598 1105 70-71 0.8749314104715751 1105 72-73 0.6510889771477153 1105 74-75 0.7182841969077494 1105 76-77 0.7625168420045085 1105 78-79 0.8090944280921271 1105 80-81 0.9841868522254522 1105 82-83 0.8577272627803367 1105 84-85 1.0186788498399544 1105 86-87 1.2299258554816355 1105 88-89 1.4272312942657308 1105 90-91 1.4124141121112856 1105 92-93 1.4514048591275994 1105 94-95 1.672484600204502 1105 96-97 1.8925615903522193 1105 98-99 2.2200806509119353 1105 100-101 2.782755095020736 1106 1 0.03618036081257259 1106 2 0.207361736122607 1106 3 0.18644000560089324 1106 4 0.1692028444447402 1106 5 0.11642180124196955 1106 6 0.05889145951903174 1106 7 0.13413117837719568 1106 8 0.09461780715619028 1106 9 0.06792438387157063 1106 10-11 -0.08173016580564507 1106 12-13 -0.19889485675606267 1106 14-15 0.08724117300774026 1106 16-17 0.22274023412803956 1106 18-19 0.1862458226333814 1106 20-21 0.26372930774810044 1106 22-23 0.25683925503748384 1106 24-25 0.16280089245918106 1106 26-27 0.2904979359090376 1106 28-29 0.27334470763293695 1106 30-31 0.18896138513497363 1106 32-33 0.3025189899499665 1106 34-35 -0.1499639972615583 1106 36-37 0.31083414825433664 1106 38-39 0.3184327944819998 1106 40-41 0.2884142662808884 1106 42-43 0.3052141119782945 1106 44-45 0.22895207265815287 1106 46-47 0.3464000339582398 1106 48-49 0.4200011738339313 1106 50-51 0.4718885091632501 1106 52-53 0.5074384634688371 1106 54-55 0.30710299376622174 1106 56-57 0.44203740394205937 1106 58-59 0.5540467037973542 1106 60-61 0.4854791834069587 1106 62-63 0.4254635043508159 1106 64-65 0.4176954806514388 1106 66-67 0.42387599152501565 1106 68-69 0.5604524577399772 1106 70-71 0.5942109812662864 1106 72-73 0.537921663926646 1106 74-75 0.5761973224562205 1106 76-77 0.3486112013036937 1106 78-79 0.475520306082295 1106 80-81 0.4494145489377068 1106 82-83 0.42134578664270705 1106 84-85 0.6054411611336583 1106 86-87 0.6685674855936341 1106 88-89 0.7108851919824879 1106 90-91 0.8241327817717234 1106 92-93 0.9019459712179874 1106 94-95 0.8293825018100485 1106 96-97 1.1748187034495032 1106 98-99 1.4818976443677414 1106 100-101 1.882313221512387 1107 1 0.17573329391940007 1107 2 0.18709601307774193 1107 3 0.20645543571374958 1107 4 0.10282873009073512 1107 5 0.11172996325445439 1107 6 0.08044774587384751 1107 7 0.1112387649314428 1107 8 0.15048731495036805 1107 9 0.2125626043719464 1107 10-11 0.1509231178344308 1107 12-13 0.1965893723263079 1107 14-15 0.30225588579890683 1107 16-17 0.19983966230258687 1107 18-19 0.34759409801348085 1107 20-21 0.3251026432077495 1107 22-23 0.19565694880823514 1107 24-25 0.10054506660640783 1107 26-27 0.15843701220804718 1107 28-29 0.1990849805353605 1107 30-31 0.2525310179832587 1107 32-33 0.3313334634697185 1107 34-35 0.30721405306425 1107 36-37 0.3331196090365509 1107 38-39 0.24745519893198775 1107 40-41 0.37607713370814366 1107 42-43 0.30918046989903303 1107 44-45 0.3396542043658073 1107 46-47 0.43180210661770246 1107 48-49 0.3196821965807217 1107 50-51 0.3599538463937222 1107 52-53 0.39554151396397685 1107 54-55 0.3783085621528386 1107 56-57 0.38839113158270777 1107 58-59 0.4701136012794578 1107 60-61 0.4854950383712904 1107 62-63 0.39617461335477344 1107 64-65 0.5396383202724664 1107 66-67 0.6420704712882284 1107 68-69 0.6503605728340958 1107 70-71 0.5839980752637572 1107 72-73 0.5752534858560026 1107 74-75 0.42559979041226015 1107 76-77 0.5023529420987316 1107 78-79 0.7489953509841705 1107 80-81 0.7776871213271654 1107 82-83 0.7610248513783731 1107 84-85 0.8768345877578092 1107 86-87 1.0141710733103793 1107 88-89 1.091416958250143 1107 90-91 1.1459303714477116 1107 92-93 1.3535416887151541 1107 94-95 1.2583845872766197 1107 96-97 1.4076705589729102 1107 98-99 1.9085070156060375 1107 100-101 2.264454497556862 1108 1 0.13171567533613882 1108 2 0.14507343288416763 1108 3 0.08316981386920475 1108 4 0.10832657643462085 1108 5 0.0661931594375389 1108 6 0.05421754229642062 1108 7 0.026983315902107563 1108 8 0.05869121562442814 1108 9 0.09804842220550114 1108 10-11 0.09170583619365402 1108 12-13 0.11192280900149143 1108 14-15 0.17271147313809365 1108 16-17 0.13310576335289426 1108 18-19 0.1973855217216638 1108 20-21 0.26173156005901177 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-0.2499092998615282 2316 74-75 -0.15300005342026068 2316 76-77 -0.1649785019214427 2316 78-79 -0.07076281282323649 2316 80-81 0.04703313087849281 2316 82-83 0.07143928575144187 2316 84-85 0.06789747918752198 2316 86-87 0.14480829668250195 2316 88-89 0.34878259632213826 2316 90-91 0.4883342310079115 2316 92-93 0.31918985177022563 2316 94-95 0.5904246988482917 2316 96-97 0.8466789699855681 2316 98-99 1.0607541248259515 2316 100-101 1.6206097992011905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 9.0 5 41.0 6 75.0 7 167.0 8 425.0 9 818.0 10 1431.0 11 2280.0 12 3636.0 13 5116.0 14 7040.0 15 9643.0 16 13297.0 17 18278.0 18 16412.0 19 10475.0 20 9478.0 21 9839.0 22 10528.0 23 11664.0 24 13840.0 25 16372.0 26 20481.0 27 25358.0 28 31594.0 29 40377.0 30 50729.0 31 63662.0 32 81644.0 33 107560.0 34 145512.0 35 205523.0 36 303293.0 37 418553.0 38 284817.0 39 19569.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.72969081849371 20.18353822904754 15.376980885476229 21.70979006698252 2 15.41408060846747 21.2949326017353 39.72282355198602 23.568163237811206 3 19.809180289720167 25.352811314457536 30.028282061252966 24.809726334569334 4 12.760915028276958 17.2417488540713 38.11847234002162 31.878863777630123 5 13.253242617609956 38.326678669557154 34.27641758627588 14.143661126557008 6 31.07648404273235 37.46092207072454 17.345905633243618 14.11668825329949 7 28.16157416054535 33.37686382457978 20.789968696830798 17.671593318044078 8 26.33257907699028 34.88338625375469 19.6028059560735 19.181228713181522 9 26.04312427470445 17.378821420880143 20.765779420335118 35.81227488408029 10-11 24.68954543552435 27.273409248877034 27.340950403818027 20.696094911780584 12-13 26.136180665120822 24.435753223419752 27.9984138394043 21.429652272055122 14-15 22.570820631620954 25.912871122410785 26.917874578569368 24.598433667398893 16-17 21.534324787476283 29.514999903038763 27.26509099556734 21.685584313917612 18-19 21.39112835320018 27.957011471024412 29.89006126929401 20.7617989064814 20-21 22.413338212021884 26.588878121072106 29.889545674680267 21.10823799222574 22-23 21.86217968836516 26.651678432201077 29.892520529084166 21.593621350349597 24-25 22.09862493589073 26.867818804875622 29.88670839404045 21.146847865193195 26-27 21.878295625361538 26.88097359008793 30.115225205478612 21.125505579071916 28-29 21.261697624725755 27.404019129574746 30.20647325130813 21.127809994391367 30-31 21.60288516471838 26.797161478845943 30.012939251350378 21.5870141050853 32-33 21.564588895321794 27.059797345657294 29.90635318889323 21.469260570127684 34-35 22.229205132209195 26.85963993700582 29.737194227642526 21.173960703142463 36-37 22.074067156371935 26.632801679572715 29.932009761444746 21.361121402610607 38-39 21.58602594326082 27.116925838159922 30.07301948334448 21.22402873523478 40-41 21.88486516774804 27.00515833769659 29.856405514338526 21.253570980216846 42-43 21.588664658562802 27.174167114934516 29.35665312846727 21.880515098035414 44-45 21.896908773766985 26.932590508209554 29.23794593237413 21.93255478564933 46-47 22.20708266098372 27.406424753488817 28.901592511734865 21.484900073792605 48-49 22.1794997412666 27.493791929355066 28.709022396003974 21.617685933374364 50-51 21.756927369911196 27.65298696738939 28.888400680565805 21.701684982133617 52-53 22.30930021545807 27.939201223140465 28.100386518101644 21.651112043299825 54-55 21.884074168200186 27.485065418214223 28.445185772622843 22.185674640962755 56-57 22.169803617776772 27.707081130016853 28.272773387305016 21.85034186490136 58-59 22.589186474826313 27.254910065903022 28.432708392658302 21.72319506661237 60-61 22.176131614159605 27.554826475112094 28.97653333986546 21.292508570862843 62-63 21.963123090756735 27.62050495369846 28.80651946930565 21.60985248623916 64-65 21.800942667332126 27.746554559066233 28.565060478294463 21.887442295307178 66-67 21.91507624725319 27.71874199431909 28.074258016908 22.29192374151972 68-69 21.818523270186052 27.557531183243466 28.499917838111482 22.124027708459 70-71 22.137857442489228 27.56809485462449 28.392775673548876 21.901272029337406 72-73 22.135662857867516 27.207257412001734 28.685485864933202 21.971593865197548 74-75 21.786475030440723 28.158818420185085 28.052594835742568 22.002111713631628 76-77 21.993283138032993 27.567150758389953 28.267006745440014 22.172559358137036 78-79 22.0197178326 27.493919509927302 28.284077026005004 22.202285631467696 80-81 22.108743555905296 27.86091778401732 28.01967904810604 22.010659611971345 82-83 22.04425830117446 27.59448381305428 28.40477519746961 21.956482688301655 84-85 21.885604362040816 27.63883796286019 28.173350755776287 22.30220691932271 86-87 21.836665227557813 27.652859386817152 28.156547485999305 22.35392789962573 88-89 21.8620027292036 27.611880507015403 27.993576063026843 22.532540700754154 90-91 21.85767863280928 27.40967721603133 28.270640313712203 22.462003837447185 92-93 22.01951137610751 27.252798958522312 28.360899984103277 22.366789681266898 94-95 21.7651435587631 27.156137186879004 28.442455548377023 22.636263705980873 96-97 21.530752531453714 27.115898274396212 28.76620400847952 22.58714518567056 98-99 21.651220427667024 27.244195508279116 28.059030225739377 23.045553838314483 100-101 21.294303694908187 26.84050657073599 28.779024886804507 23.08616484755132 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 209.0 1 153.0 2 142.0 3 206.0 4 198.0 5 201.5 6 391.0 7 936.5 8 1594.0 9 1979.5 10 1943.5 11 1829.0 12 1955.5 13 2176.0 14 2377.5 15 2524.5 16 2652.5 17 2808.0 18 3046.0 19 3302.0 20 3630.5 21 4117.0 22 4774.5 23 5713.0 24 6854.0 25 8271.0 26 10553.0 27 13371.5 28 15771.5 29 19079.5 30 23435.5 31 27477.5 32 32168.0 33 37574.5 34 43254.0 35 47993.0 36 53600.0 37 59926.5 38 63351.0 39 64650.5 40 65276.0 41 67277.5 42 69257.0 43 70853.5 44 72818.0 45 73697.5 46 72586.5 47 77445.0 48 92345.0 49 90917.0 50 75478.5 51 67071.5 52 62094.0 53 59000.5 54 55462.0 55 50170.5 56 44494.5 57 38852.5 58 34251.5 59 30034.0 60 25635.0 61 21130.5 62 16726.0 63 12860.0 64 9672.0 65 7497.0 66 5517.5 67 4273.0 68 3201.0 69 2027.5 70 1352.0 71 1023.0 72 847.0 73 714.0 74 557.5 75 411.5 76 281.5 77 154.5 78 76.5 79 42.5 80 27.5 81 19.0 82 12.0 83 5.0 84 3.0 85 2.0 86 3.0 87 2.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04215262106630822 2 0.0 3 0.0 4 0.0 5 9.185801200890409E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004975642317148972 14-15 2.5516114446917805E-5 16-17 0.0 18-19 0.0 20-21 1.5309668668150685E-4 22-23 4.5929006004452046E-4 24-25 5.103222889383561E-5 26-27 0.0018116441257311643 28-29 0.0034191593358869864 30-31 2.2964503002226023E-4 32-33 1.2758057223458903E-4 34-35 0.0 36-37 0.0 38-39 2.5516114446917805E-5 40-41 0.0 42-43 0.0077568987918630134 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.2964503002226023E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.0412891557534244E-4 84-85 2.2964503002226023E-4 86-87 0.0 88-89 0.0 90-91 0.008700995026398973 92-93 0.0011482251501113013 94-95 0.0 96-97 0.0 98-99 3.827417167037671E-4 100-101 0.010946413097727738 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1959546.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.97371118342926 #Duplication Level Percentage of deduplicated Percentage of total 1 75.38639096848068 39.181105113568954 2 13.650683368229952 14.189533496736495 3 4.67938174769484 7.296145064151059 4 2.069155708529121 4.301668047545459 5 1.1065690226929012 2.875624939498521 6 0.6690087687435455 2.0862521115515187 7 0.41766276240073047 1.5195238649562162 8 0.3104562268883011 1.29084498171118 9 0.22932899157733955 1.0727170896804954 >10 1.300624141932654 12.61622051857841 >50 0.11514433937986662 4.1320813191751435 >100 0.061254863489414016 5.619330836250476 >500 0.0025640077040379786 0.919962159646794 >1k 0.001479235213868064 1.7005640701387807 >5k 2.9584704277361285E-4 1.1984263868104816 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8463 0.4318857531285308 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8389 0.428109368190387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6530 0.33324045467674657 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4478 0.22852232098659586 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3321 0.16947803215642807 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3310 0.16891667763859589 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2630 0.13421476199078766 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2487 0.12691715325896916 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2442 0.12462070295874658 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2226 0.11359774151767808 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2100 0.10716768067705479 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.5516114446917808E-5 0.0 16-17 0.0 0.0 0.0 7.654834334075342E-5 0.0 18-19 0.0 0.0 0.0 1.0206445778767123E-4 0.0 20-21 0.0 0.0 0.0 1.0206445778767123E-4 0.0 22-23 0.0 0.0 0.0 1.0206445778767123E-4 0.0 24-25 0.0 0.0 0.0 2.551611444691781E-4 0.0 26-27 0.0 0.0 0.0 4.337739455976027E-4 0.0 28-29 0.0 0.0 0.0 0.0010206445778767122 0.0 30-31 0.0 0.0 0.0 0.002551611444691781 0.0 32-33 0.0 0.0 0.0 0.004924610088255137 0.0 34-35 0.0 0.0 0.0 0.007731382677416096 0.0 36-37 0.0 0.0 0.0 0.010410574694342464 0.0 38-39 0.0 0.0 0.0 0.012936670024587328 0.0 40-41 0.0 0.0 0.0 0.01755508673947945 0.0 42-43 0.0 0.0 0.0 0.02311759968890753 0.0 44-45 0.0 0.0 0.0 0.028731144867229448 0.0 46-47 0.0 0.0 0.0 0.03587565691236644 0.0 48-49 0.0 0.0 0.0 0.0429946528430565 0.0 50-51 0.0 0.0 0.0 0.05450242045861643 0.0 52-53 0.0 0.0 0.0 0.083820435958125 0.0 54-55 0.0 0.0 0.0 0.12288560717635616 0.0 56-57 0.0 0.0 0.0 0.15888884466095718 0.0 58-59 0.0 0.0 0.0 0.21729523062995204 0.0 60-61 0.0 0.0 0.0 0.29649724987318493 0.0 62-63 0.0 0.0 0.0 0.3531175078308955 0.0 64-65 0.0 0.0 0.0 0.3999395778409897 0.0 66-67 0.0 0.0 0.0 0.5230548300473681 0.0 68-69 0.0 0.0 0.0 0.6545904000212295 0.0 70-71 0.0 0.0 0.0 0.7211364264987911 0.0 72-73 0.0 0.0 0.0 0.8405773582248133 0.0 74-75 0.0 0.0 0.0 1.0230686087491696 0.0 76-77 0.0 0.0 0.0 1.2173227880335546 0.0 78-79 0.0 0.0 0.0 1.4044069391583562 0.0 80-81 0.0 0.0 0.0 1.6367311611975426 0.0 82-83 0.0 0.0 0.0 1.8921729829256368 0.0 84-85 0.0 0.0 0.0 2.1544020910966113 0.0 86-87 0.0 0.0 0.0 2.5015743442613747 0.0 88-89 0.0 0.0 0.0 2.900033987464443 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9110 0.0 23.83991 1 GAGTACT 7890 0.0 22.609741 12-13 TACATGG 9480 0.0 22.396141 2 ACATGGG 9335 0.0 21.828152 3 GTACTTT 8360 0.0 21.221174 14-15 AGTACTT 8275 0.0 21.098522 12-13 CATGGGA 6040 0.0 19.816988 4 GTATAGG 795 0.0 19.726662 1 AGAGTAC 14215 0.0 19.116652 10-11 TATAGCG 180 1.2343106E-4 18.47139 5 GTATCAA 20660 0.0 18.103027 1 ACTTTTT 9855 0.0 17.929176 16-17 GTATACG 160 0.0011917825 17.811695 4 TACTTTT 9700 0.0 17.79991 14-15 CCGAGCG 200 2.7582294E-4 16.627644 9 ACTCCGT 200 2.7625283E-4 16.62425 6 CTAGGAC 1315 0.0 16.25402 3 CAACGCA 23070 0.0 16.203213 5 TCAACGC 23100 0.0 16.161608 4 ATCAACG 23160 0.0 16.09923 3 >>END_MODULE