FastQCFastQC Report
Thu 26 May 2016
SRR937238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929022
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73260.3797779392873694No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67330.34903697313975685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48010.24888259439239158No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42580.2207336152724023No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT36460.1890076940542928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32240.16713132354115195No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28990.15028340786160035No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26960.13975994052944962No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24510.12705920409409535No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21400.11093704478227827No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20370.10559755150537423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95700.026.7334371
TACATGG97550.025.679532
ACATGGG96000.024.9051513
TCGCGCT1750.023.06691760-61
GAGTACT58300.022.16035712-13
CATGGGA57250.021.2581124
GTACTTT61550.020.60304314-15
GTGTAGG9300.020.453211
CATGGGG37250.020.2922884
AGTACTT62750.019.7561912-13
AGAGTAC113750.019.45932610-11
CTCGCGC2100.019.22243160-61
GTATATG9750.019.0214861
GTATCAA173450.018.7528061
CGATCGT2300.018.58670224-25
CATGGGC18300.018.4445254
AATTCGC2200.018.35011578-79
TCGCGGA2101.8189894E-1218.0921782-83
GAGTACA78450.018.0031281
GTGTAGC10250.017.629671
Produced by FastQC (version 0.11.5)