FastQCFastQC Report
Thu 26 May 2016
SRR937238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929022
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86290.4473251212272333No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT84660.43887524351718127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66270.3435419606411954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46470.24089927434731173No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34000.17625511787838605No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33680.1745962461807071No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25840.1339538895875734No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24680.1279404796834873No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23890.12384514017984241No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21630.112129358814985No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC21460.11124808322559307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG89300.023.9974941
TAGACGG1050.00219706522.6185045
GAGTACT76750.022.43664212-13
TACATGG94250.022.3257262
ACATGGG91200.022.2911323
GTACTTT81150.021.39295414-15
AGTACTT80950.020.89110412-13
TCGCGCT1900.019.99960-61
GTATAGG6700.019.8574751
AGAGTAC133800.019.2784410-11
ATTCGCG1857.2759576E-1219.25629480-81
CTCGCGC2001.8189894E-1218.9990560-61
GTATCAA196950.018.8906361
CATGGGA58850.018.8058364
CATGGGG33050.018.6833634
ACCGCTC2302.0577609E-618.5884388
CGATCGT2101.8189894E-1218.09574124-25
CGCGCTT2250.017.94354862-63
TACTTTT94550.017.78335214-15
ACTTTTT98450.017.77890416-17
Produced by FastQC (version 0.11.5)