FastQCFastQC Report
Thu 26 May 2016
SRR937237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983110
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75040.37839555042332496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68350.34466065926751416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47920.24164065533429815No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44020.22197457528830977No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT37160.18738244474587895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32830.1655480533102047No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28540.14391536525961746No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28440.1434111067968998No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25780.12999783168861032No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22540.11365985749655844No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21340.10760875594394662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG93650.025.5874611
TACATGG98800.023.9576932
ACATGGG96450.023.9494573
GAGTACT60000.022.08661312-13
ATGGGCG2405.8917067E-921.7842735
CATGGGA58950.020.4791324
GTACTTT64900.020.23506714-15
CATGGGG36450.020.2113724
AGTACTT65000.020.09534512-13
ACTGCGT1951.0333959E-519.486898
AGAGTAC118600.019.26331710-11
GTATCAA175050.019.1211951
GTGCGTC1300.007567862518.2680369
AGTACAT71850.018.191922
GTACCTG17050.018.1256431
ATGGGAT26150.017.9938935
TACCTGG15400.017.9011062
CTATACG1350.009369370517.6034534
GAGTACA81400.017.4643021
GTATAGG6650.017.1590821
Produced by FastQC (version 0.11.5)