##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937237_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1983110 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.539402252018295 31.0 31.0 34.0 30.0 34.0 2 31.620331701216777 33.0 31.0 34.0 30.0 34.0 3 31.67467462722693 33.0 31.0 34.0 30.0 34.0 4 35.31574446198143 37.0 35.0 37.0 33.0 37.0 5 35.2889849781404 37.0 35.0 37.0 33.0 37.0 6 35.37268734462536 37.0 35.0 37.0 33.0 37.0 7 35.305988573503235 37.0 35.0 37.0 33.0 37.0 8 35.293959992133566 37.0 35.0 37.0 33.0 37.0 9 36.88113316961742 39.0 37.0 39.0 33.0 39.0 10-11 36.78465995330566 39.0 37.0 39.0 33.0 39.0 12-13 36.67473362546707 39.0 37.0 39.0 32.0 39.0 14-15 37.78683406366767 40.0 37.5 41.0 32.5 41.0 16-17 37.7351957279223 40.0 37.0 41.0 32.0 41.0 18-19 37.697193045267284 40.0 37.0 41.0 32.0 41.0 20-21 37.61701317627363 40.0 37.0 41.0 32.0 41.0 22-23 37.48394516693476 40.0 37.0 41.0 32.0 41.0 24-25 37.33220093691222 39.5 37.0 41.0 32.0 41.0 26-27 37.166791050420805 39.0 36.5 41.0 31.0 41.0 28-29 36.994707555304544 39.0 36.0 41.0 31.0 41.0 30-31 37.01840240833842 39.0 36.0 41.0 31.0 41.0 32-33 36.890942257363434 39.0 36.0 41.0 30.5 41.0 34-35 36.69910115929021 39.0 36.0 41.0 30.0 41.0 36-37 36.70969588172113 39.0 36.0 41.0 30.0 41.0 38-39 36.685861853351554 39.0 36.0 41.0 30.0 41.0 40-41 36.55560004235771 39.0 35.5 41.0 30.0 41.0 42-43 36.335126140254445 39.0 35.0 41.0 30.0 41.0 44-45 36.11852796869564 39.0 35.0 41.0 29.5 41.0 46-47 35.8226185133452 39.0 35.0 40.5 28.5 41.0 48-49 35.54712597889174 39.0 35.0 40.0 27.0 41.0 50-51 34.62606965826404 38.0 34.0 39.5 25.5 40.5 52-53 34.77515795896345 38.0 34.0 39.5 26.0 40.5 54-55 35.147096479771676 38.5 35.0 40.0 26.0 41.0 56-57 34.931539854067594 38.0 34.0 40.0 26.0 41.0 58-59 34.69611443641553 38.0 34.0 40.0 25.5 41.0 60-61 34.40219755838052 38.0 34.0 40.0 24.5 41.0 62-63 34.098726243123174 37.5 34.0 40.0 23.5 41.0 64-65 33.747117658627104 37.0 33.0 40.0 23.0 41.0 66-67 33.353222967964456 36.5 33.0 39.0 22.0 41.0 68-69 32.86033855913187 36.0 32.0 39.0 20.5 40.5 70-71 32.42413355789644 35.5 32.0 39.0 20.0 40.0 72-73 32.003687137879396 35.0 31.0 38.0 18.5 40.0 74-75 31.54745601605559 35.0 31.0 37.0 17.5 39.0 76-77 31.107204340656846 35.0 31.0 37.0 14.0 39.0 78-79 30.629060415206418 34.5 30.0 36.0 9.0 39.0 80-81 30.156751768686558 34.0 30.0 36.0 7.0 37.5 82-83 29.70199257731543 34.0 29.0 35.0 4.0 37.0 84-85 29.229126473065037 34.0 29.0 35.0 2.0 36.5 86-87 28.80374033714721 34.0 29.0 35.0 2.0 36.0 88-89 28.395821966507153 34.0 28.5 35.0 2.0 36.0 90-91 27.940323280100447 33.5 27.0 35.0 2.0 35.0 92-93 27.4861026367675 33.0 26.5 35.0 2.0 35.0 94-95 26.92533142387462 33.0 25.5 35.0 2.0 35.0 96-97 26.283386196428843 32.5 25.0 35.0 2.0 35.0 98-99 25.54479126220936 32.0 22.0 34.5 2.0 35.0 100-101 23.660150974983736 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13246035635394904 1101 2 0.1752662764528914 1101 3 0.24032563372490046 1101 4 0.20568379724106478 1101 5 0.32769798931308713 1101 6 0.08000455158986597 1101 7 0.2130143752677398 1101 8 0.13726909589418312 1101 9 -0.012427271371016957 1101 10-11 0.15815739030571052 1101 12-13 0.2807096497037591 1101 14-15 0.4433659586443355 1101 16-17 0.22862031462759091 1101 18-19 0.38853255542695564 1101 20-21 0.3391787672001456 1101 22-23 0.19778216958268757 1101 24-25 0.34105288016090896 1101 26-27 0.2599538064310565 1101 28-29 0.47293824541048934 1101 30-31 0.27675757309093285 1101 32-33 0.25720155529128874 1101 34-35 0.2882366371795584 1101 36-37 0.25933797627558874 1101 38-39 0.3796736401587495 1101 40-41 0.3858258701658528 1101 42-43 0.39094055801704286 1101 44-45 0.2973313162535831 1101 46-47 0.4438513798368149 1101 48-49 0.36887176771988806 1101 50-51 0.3661666728861448 1101 52-53 0.3566179214929832 1101 54-55 0.3802466073565114 1101 56-57 0.35943534465756244 1101 58-59 0.38518142532218747 1101 60-61 0.39759270520617207 1101 62-63 0.5629862437063977 1101 64-65 0.4964664478314731 1101 66-67 0.646419754210676 1101 68-69 0.46229123437199604 1101 70-71 0.5004711711015446 1101 72-73 0.5241337612546957 1101 74-75 0.5676744915046505 1101 76-77 0.5310246834564332 1101 78-79 0.6056936477943822 1101 80-81 0.64160376776859 1101 82-83 0.7801078383381714 1101 84-85 0.7685543815777152 1101 86-87 0.8449528698166056 1101 88-89 0.7571548032681861 1101 90-91 0.8373531010396071 1101 92-93 0.7669900431762109 1101 94-95 0.6939191956513966 1101 96-97 0.798684055464129 1101 98-99 0.7051532436769818 1101 100-101 1.0119149576064643 1102 1 0.08116452066990121 1102 2 0.029092675774322174 1102 3 0.04434336929910998 1102 4 -0.0194941513372342 1102 5 0.09966191881378705 1102 6 0.1370007352883036 1102 7 0.09938995719183197 1102 8 0.14967722257090088 1102 9 0.2583881194383224 1102 10-11 0.19744666440059433 1102 12-13 0.31034898379886755 1102 14-15 0.27091238553590813 1102 16-17 0.3610801157733974 1102 18-19 0.38300354358716504 1102 20-21 0.4098652975709456 1102 22-23 0.5322582652547183 1102 24-25 0.5112669656882787 1102 26-27 0.4817275723219936 1102 28-29 0.4661685772554307 1102 30-31 0.36159451707898427 1102 32-33 0.28932363524729965 1102 34-35 0.42153162074301775 1102 36-37 0.48025154336445297 1102 38-39 0.666045834447452 1102 40-41 0.4767131309629633 1102 42-43 0.42528388444046783 1102 44-45 0.32951554628966306 1102 46-47 0.321291421947727 1102 48-49 0.3577605842234064 1102 50-51 0.3634410596264246 1102 52-53 0.43950476013861106 1102 54-55 0.541216096488057 1102 56-57 0.4470635699144694 1102 58-59 0.37739885418510255 1102 60-61 0.500754541669373 1102 62-63 0.5433481213423974 1102 64-65 0.35254223705280197 1102 66-67 0.45613562280691156 1102 68-69 0.47009882189350805 1102 70-71 0.42179477236643237 1102 72-73 0.4014627931597019 1102 74-75 0.19192201299334144 1102 76-77 0.3350131029551413 1102 78-79 0.46368371159919164 1102 80-81 0.4159628857846869 1102 82-83 0.5202561385921136 1102 84-85 0.4995758709125262 1102 86-87 0.5075421698399261 1102 88-89 0.6344172551083886 1102 90-91 0.60419883463857 1102 92-93 0.524462580528148 1102 94-95 0.3781368538311689 1102 96-97 0.6414767960220722 1102 98-99 0.6700277643591477 1102 100-101 1.100266182432101 1103 1 0.09922429579908609 1103 2 -0.05184134307743449 1103 3 0.15928844992542324 1103 4 -0.04621378988206715 1103 5 -0.04824517266656869 1103 6 -0.04737645564416226 1103 7 -0.035344274586101676 1103 8 0.10189866579491991 1103 9 0.2501060701537057 1103 10-11 0.16340768852357002 1103 12-13 0.2487464546513607 1103 14-15 0.29708088284836975 1103 16-17 0.27696194174519206 1103 18-19 0.18187269785478577 1103 20-21 0.14814526952700646 1103 22-23 0.16021196679453453 1103 24-25 0.2739990422378398 1103 26-27 -0.006946442894438576 1103 28-29 0.12251741265311011 1103 30-31 0.24714799530598697 1103 32-33 0.04400995863542079 1103 34-35 0.24129740194837979 1103 36-37 0.3043913995094485 1103 38-39 0.3099774384047649 1103 40-41 0.34993247089181523 1103 42-43 0.2847792234345192 1103 44-45 0.058682537538921054 1103 46-47 0.03076793515175069 1103 48-49 0.03359541063664295 1103 50-51 -0.07198972638277468 1103 52-53 -0.04795466703804152 1103 54-55 0.04955397397760919 1103 56-57 0.2654407868135351 1103 58-59 0.2752871198754292 1103 60-61 0.3318540976403952 1103 62-63 0.3984908078475584 1103 64-65 0.33421046171588387 1103 66-67 0.41707665264253535 1103 68-69 0.2807652548607109 1103 70-71 0.24813854427576132 1103 72-73 0.3310270734951857 1103 74-75 0.3972626948764386 1103 76-77 0.3660931839709498 1103 78-79 0.5303412899534301 1103 80-81 0.4506153227507461 1103 82-83 0.6786446509652038 1103 84-85 0.5011507456236473 1103 86-87 0.8227008258083508 1103 88-89 0.8759962844766207 1103 90-91 0.6585002030787521 1103 92-93 0.7677748809098546 1103 94-95 0.8540450359090137 1103 96-97 0.8887431614760999 1103 98-99 0.9560142357569283 1103 100-101 1.2981090065602707 1104 1 0.30900195039381373 1104 2 0.28098386017460086 1104 3 0.3661435866586089 1104 4 0.2720881317735149 1104 5 0.2876164927682723 1104 6 0.3301549560335957 1104 7 0.23673320755236205 1104 8 0.3223131767582075 1104 9 0.31247980752744553 1104 10-11 0.37701787576180834 1104 12-13 0.42062222454541853 1104 14-15 0.5453347631144396 1104 16-17 0.38606208956485233 1104 18-19 0.4489951418064919 1104 20-21 0.5786700293200653 1104 22-23 0.548387933781477 1104 24-25 0.5036060883082953 1104 26-27 0.6263745721338978 1104 28-29 0.5353777230743972 1104 30-31 0.43454174992535144 1104 32-33 0.4540001039914259 1104 34-35 0.49324523392670017 1104 36-37 0.36978158182036225 1104 38-39 0.5183200104668444 1104 40-41 0.4542669744641259 1104 42-43 0.4482138748264859 1104 44-45 0.5806953485761213 1104 46-47 0.5858030423210039 1104 48-49 0.6420088129524828 1104 50-51 0.5696907983770245 1104 52-53 0.51680728171781 1104 54-55 0.6034142228073733 1104 56-57 0.7418065788805492 1104 58-59 0.6219303817625885 1104 60-61 0.672513742873214 1104 62-63 0.7763286373622194 1104 64-65 0.629729678271687 1104 66-67 0.670846053972916 1104 68-69 0.7553448927025457 1104 70-71 0.6006884334110154 1104 72-73 0.6977876672374919 1104 74-75 0.8324884278155871 1104 76-77 0.8456894515245352 1104 78-79 0.8187081906678877 1104 80-81 0.9937586462294377 1104 82-83 0.985609112655272 1104 84-85 1.0342916054413394 1104 86-87 0.9119022916866477 1104 88-89 0.9588076296355261 1104 90-91 1.0225769461189103 1104 92-93 0.855348790739864 1104 94-95 0.9529025131201507 1104 96-97 1.0778953541343874 1104 98-99 1.137715393677599 1104 100-101 1.3287107557595803 1105 1 0.2861494685795236 1105 2 0.26943058674712717 1105 3 0.3501776046303675 1105 4 0.15687632176067723 1105 5 0.25640660830100614 1105 6 0.20907290639731002 1105 7 0.16960654944367093 1105 8 0.2795767625519616 1105 9 0.29603243910639065 1105 10-11 0.3073238235581286 1105 12-13 0.43612019202509344 1105 14-15 0.3852537297383165 1105 16-17 0.44594120809288285 1105 18-19 0.4513686441599418 1105 20-21 0.5546943125892199 1105 22-23 0.567884082690334 1105 24-25 0.561700065629644 1105 26-27 0.447486039827524 1105 28-29 0.4808607140885215 1105 30-31 0.5724121500109334 1105 32-33 0.44597699744456776 1105 34-35 0.6219626066267452 1105 36-37 0.585061588238851 1105 38-39 0.6549535149597929 1105 40-41 0.5904993236297216 1105 42-43 0.34772446532713275 1105 44-45 0.4521451239291352 1105 46-47 0.5527077278152248 1105 48-49 0.6408588343474335 1105 50-51 0.4380182480988921 1105 52-53 0.6041120527918338 1105 54-55 0.5692692185283761 1105 56-57 0.6241811509815278 1105 58-59 0.718007082661174 1105 60-61 0.7125757882305095 1105 62-63 0.8086350130575468 1105 64-65 0.6821071921783997 1105 66-67 0.8745527291975606 1105 68-69 0.7967441224843199 1105 70-71 0.7446497085500781 1105 72-73 0.7842633839683444 1105 74-75 0.9009990718036001 1105 76-77 1.0577000420251785 1105 78-79 1.0950774675185535 1105 80-81 1.0275225529474525 1105 82-83 1.1322514425654155 1105 84-85 0.976685700434917 1105 86-87 1.1651516498381262 1105 88-89 1.1682909415739076 1105 90-91 1.2476117129139475 1105 92-93 1.1427698968588196 1105 94-95 1.2376358785459125 1105 96-97 1.3428383900779046 1105 98-99 1.536684424486328 1105 100-101 1.9427993308558555 1106 1 0.1298105638853535 1106 2 0.10074874701265557 1106 3 0.06162260225957539 1106 4 0.0872935811253086 1106 5 0.12719845277705844 1106 6 0.15121076794306276 1106 7 0.14011982587800276 1106 8 0.033310759707013915 1106 9 0.05966449221212855 1106 10-11 0.06095409856998657 1106 12-13 -0.012078006173105393 1106 14-15 0.0026449696624482044 1106 16-17 -0.06422356194030954 1106 18-19 0.024974322206404054 1106 20-21 0.0710824112141566 1106 22-23 0.20965743498999956 1106 24-25 0.08550429749308819 1106 26-27 0.2080805158825214 1106 28-29 0.31066749455319354 1106 30-31 0.3912614269194208 1106 32-33 0.4013468409723018 1106 34-35 0.324161516062496 1106 36-37 0.1054578068258607 1106 38-39 0.14123255715988847 1106 40-41 0.3601324435917945 1106 42-43 0.3365076564129552 1106 44-45 0.36834504095141796 1106 46-47 0.378884233268046 1106 48-49 0.345013647054877 1106 50-51 0.24223529409225364 1106 52-53 0.3817614126780384 1106 54-55 0.3549866794103167 1106 56-57 0.21774593286867372 1106 58-59 0.0347448730831772 1106 60-61 0.13512498216128677 1106 62-63 0.3170957764525255 1106 64-65 0.19282020782562626 1106 66-67 0.23273492455081168 1106 68-69 0.23686681096226891 1106 70-71 0.3004522215894383 1106 72-73 0.41004870876682276 1106 74-75 0.3800397901535355 1106 76-77 0.46286836824613786 1106 78-79 0.4518520052141426 1106 80-81 0.6118782902637143 1106 82-83 0.44598869955925124 1106 84-85 0.49482225804516133 1106 86-87 0.3704622235697492 1106 88-89 0.6803523450826816 1106 90-91 0.7995765066550575 1106 92-93 0.8797527678877408 1106 94-95 0.9525111156250965 1106 96-97 1.0820290610119976 1106 98-99 1.0784770394697354 1106 100-101 1.4289153811166457 1107 1 0.06720928487615296 1107 2 0.01471825720807729 1107 3 0.09996394145744603 1107 4 0.011550144969667997 1107 5 0.11598059680545703 1107 6 0.17755054455050612 1107 7 0.06753894735494015 1107 8 0.11987175860740962 1107 9 0.08487452558597397 1107 10-11 0.045773098512107424 1107 12-13 0.036762590312029886 1107 14-15 0.24698841642827318 1107 16-17 0.07779251730810444 1107 18-19 0.1338739739995276 1107 20-21 0.23649936724942222 1107 22-23 0.14255466573252562 1107 24-25 0.09132000345601199 1107 26-27 0.01955627161684248 1107 28-29 -0.037769071027383916 1107 30-31 0.2068456138901169 1107 32-33 0.13208936079405476 1107 34-35 0.07549321446325052 1107 36-37 0.26437723213982167 1107 38-39 0.3242545781101711 1107 40-41 0.25789972371988057 1107 42-43 0.2750367234885971 1107 44-45 0.23650087076410387 1107 46-47 0.3025656770244396 1107 48-49 0.3547967170627899 1107 50-51 0.18615867411038067 1107 52-53 0.3040514757603958 1107 54-55 0.3679720952329859 1107 56-57 0.35602976005775844 1107 58-59 0.10673435453465174 1107 60-61 0.2819270974457311 1107 62-63 0.43826164253273703 1107 64-65 0.40787050973352734 1107 66-67 0.30647870312596126 1107 68-69 0.3599473325973932 1107 70-71 0.5011558695628935 1107 72-73 0.39258591674568066 1107 74-75 0.5463845342552425 1107 76-77 0.4742610510788623 1107 78-79 0.4207177526532284 1107 80-81 0.5285720852364157 1107 82-83 0.5699668666985538 1107 84-85 0.6226164735668931 1107 86-87 0.8095597793696605 1107 88-89 0.7852104821276491 1107 90-91 0.9400999262202312 1107 92-93 1.073569443235467 1107 94-95 0.8841025705704055 1107 96-97 0.988322466301657 1107 98-99 1.1175882197077875 1107 100-101 1.4214635368741142 1108 1 -0.039494382698599395 1108 2 -0.0846975292485368 1108 3 -0.09950110306302307 1108 4 -0.18239326418308366 1108 5 0.010153733522393793 1108 6 -0.07331819346435253 1108 7 -0.04446587735411356 1108 8 -0.11476521053747035 1108 9 0.04990237575111678 1108 10-11 -0.04072898204956488 1108 12-13 0.059990106640860574 1108 14-15 0.20291965733354544 1108 16-17 0.1329681362872961 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quality scores pass #Quality Count 2 7597.0 3 1472.0 4 1395.0 5 1671.0 6 1854.0 7 2136.0 8 2584.0 9 3354.0 10 4093.0 11 5239.0 12 6635.0 13 8167.0 14 9614.0 15 11765.0 16 14728.0 17 18328.0 18 16022.0 19 11988.0 20 12304.0 21 13012.0 22 14112.0 23 16069.0 24 18288.0 25 20856.0 26 24144.0 27 28744.0 28 33870.0 29 41347.0 30 50688.0 31 63264.0 32 79427.0 33 103496.0 34 138430.0 35 191410.0 36 273178.0 37 377684.0 38 312266.0 39 41870.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41982000574622 19.526371909889566 14.722815728454714 22.3309923559095 2 14.854403003263581 20.66801781165079 40.45162223361661 24.025956951469016 3 19.438662920643782 25.246768246185713 29.63995096998125 25.67461786318926 4 12.134558739870801 16.61019177528033 38.49598624578082 32.75926323906805 5 13.219597015053663 38.26112061385657 34.20887242383043 14.310409947259343 6 30.978970202354766 36.928342855583665 17.779852848514615 14.31283409354695 7 28.11739326633049 33.21587875412915 20.92306868237792 17.74365929716244 8 26.141415162272448 34.609952373131875 19.871849615321448 19.37678284927423 9 26.16323636938771 17.37670308436202 20.829688784592896 35.63037176165737 10-11 24.555443030810217 27.109485229611124 27.523342146648716 20.811729592929943 12-13 25.907648132248312 24.452565995038462 27.970414736436762 21.669371136276464 14-15 22.570583759192132 25.84049217386559 26.82160112129624 24.767322945646033 16-17 21.564574922261677 29.507287960141788 27.086187907562454 21.841949210034088 18-19 21.451537480354705 28.118495515908613 29.404493362978855 21.025473640757824 20-21 22.521596617403667 26.81744949588734 29.241161827655038 21.419792059053954 22-23 21.95105806905713 26.889513695742078 29.286688573925378 21.87273966127541 24-25 22.080925322768362 27.146166125509 29.233629296431037 21.539279255291603 26-27 21.83547365982313 27.108385183478394 29.545853617989305 21.510287538709175 28-29 21.301368513230614 27.6744067358966 29.538925795688648 21.485298955184135 30-31 21.635777904112185 27.099084075547974 29.332171056787317 21.932966963552524 32-33 21.491896879807907 27.27105043797201 29.3786778240648 21.858374858155287 34-35 22.241790946734948 27.24950923794822 28.969235472306366 21.53946434301046 36-37 22.040023252705932 26.913895512889663 29.314729821191136 21.731351413213268 38-39 21.641848542880954 27.434807112314232 29.34831237010872 21.5750319746961 40-41 21.826430449635385 27.309809140337038 29.263118965139622 21.600641444887955 42-43 21.548797052672317 27.417660270668513 28.768198811237088 22.265343865422075 44-45 21.8334722959145 27.2755501654918 28.698703858106573 22.192273680487126 46-47 22.15299288262431 27.6576474841354 28.3986649962699 21.790694636970386 48-49 22.081161267209044 27.692197691725234 28.27736386441608 21.94927717664964 50-51 21.625945677160686 27.820911195412744 28.565628011499474 21.987515115927096 52-53 22.167603197360766 28.079294223595923 27.813319169559474 21.93978340948383 54-55 21.75868803380685 27.599992015243807 28.22497606468259 22.416343886266752 56-57 21.978851124282357 27.927281857698006 27.994170949117812 22.099696068901828 58-59 22.46575024158323 27.45117947676583 28.09424802073499 21.98882226091595 60-61 22.119890748009368 27.685220291084363 28.62845709727474 21.56643186363153 62-63 21.779526735639866 27.85235602861279 28.540622981619524 21.82749425412782 64-65 21.672509863552094 27.995236194815888 28.188869972872077 22.143383968759945 66-67 21.81425704206228 27.855886079067126 27.801472009215534 22.528384869655063 68-69 21.681245374428197 27.766994950706874 28.269373094517007 22.282386580347918 70-71 22.039566499854878 27.732015611986903 28.06206904042763 22.166348847730585 72-73 22.01481197033564 27.419779020459618 28.400026779274356 22.165382229930387 74-75 21.554152265655276 28.358949071826288 27.913643948897526 22.17325471362091 76-77 21.783978160442732 27.78791114012453 27.991532775757 22.436577923675742 78-79 21.88252983894071 27.712661977588017 28.039711824269194 22.365096359202084 80-81 21.967359702544385 28.036883889757537 27.83950632876221 22.15625007893587 82-83 21.964131428831276 27.716643845652474 28.197224088885747 22.122000636630506 84-85 21.857382850812307 27.865617689976315 27.892644491723107 22.384354967488274 86-87 21.816114199946902 27.887804659953154 27.936025356660128 22.360055783439815 88-89 21.8231809026705 27.74124216915163 27.693775209717312 22.741801718460554 90-91 21.766169374161183 27.629226036911586 28.00995679645581 22.594647792471424 92-93 22.13075804338122 27.564968127603773 28.092852716511374 22.211421112503636 94-95 21.818102358095192 27.380161443605132 28.150749747254917 22.650986451044755 96-97 21.543903008306618 27.36252555085264 28.552136874768237 22.54143456607251 98-99 21.566954201130635 27.61780904902435 27.88040971261206 22.93482703723296 100-101 21.480058235045696 27.134586201810095 28.525170188991112 22.860185374153097 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2556.0 1 1459.5 2 349.0 3 315.0 4 270.5 5 286.5 6 458.5 7 837.0 8 1345.0 9 1687.0 10 1692.5 11 1645.0 12 1797.0 13 2008.0 14 2200.0 15 2305.5 16 2388.5 17 2566.0 18 2760.5 19 2957.5 20 3243.0 21 3765.5 22 4604.0 23 5570.0 24 6605.5 25 8087.0 26 10422.0 27 13286.5 28 15775.5 29 19124.0 30 23562.5 31 27901.5 32 32771.0 33 38000.0 34 43956.5 35 48728.5 36 54077.0 37 60415.5 38 63622.0 39 64714.0 40 65258.0 41 67443.0 42 69695.5 43 71661.0 44 73579.0 45 74511.0 46 74072.0 47 78666.5 48 92793.5 49 91645.0 50 76573.5 51 68004.0 52 62884.5 53 59805.5 54 56416.0 55 51014.0 56 45152.0 57 39770.5 58 35415.0 59 30962.5 60 26206.5 61 21678.5 62 17323.5 63 13361.0 64 10146.5 65 7863.5 66 5820.5 67 4485.0 68 3364.0 69 2248.5 70 1631.5 71 1236.5 72 963.0 73 794.5 74 572.5 75 419.0 76 304.0 77 190.5 78 108.5 79 69.5 80 48.0 81 29.5 82 21.0 83 14.5 84 9.0 85 8.0 86 7.0 87 3.5 88 4.5 89 5.5 90 2.5 91 0.5 92 2.0 93 3.5 94 2.0 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3105223613415292 2 0.2787540781903172 3 0.279661743423209 4 0.2791070591142196 5 0.2682150763195183 6 0.20477936170963787 7 0.21123387003242383 8 0.21103216664733673 9 0.2081074675635744 10-11 0.21612517712078505 12-13 0.2161756029670568 14-15 0.2002914613914508 16-17 0.2115616380331903 18-19 0.1990056023115208 20-21 0.2036447801685232 22-23 0.20218243062664198 24-25 0.19492110876350782 26-27 0.2062164983283832 28-29 0.2067963955605085 30-31 0.213553458960925 32-33 0.21549485404238794 34-35 0.20397254816928967 36-37 0.20097221031611964 38-39 0.19214768721856074 40-41 0.1930553524514525 42-43 0.19290407491263722 44-45 0.20977152049054265 46-47 0.19865262138761844 48-49 0.20672075679110083 50-51 0.21365431065346854 52-53 0.2195037088209933 54-55 0.21882295989632447 56-57 0.18728159305333542 58-59 0.17452385394657885 60-61 0.18402912596880658 62-63 0.1838274225837195 64-65 0.1815330465783542 66-67 0.19376131429925725 68-69 0.1827936927351483 70-71 0.18826489705563484 72-73 0.20006454508322788 74-75 0.19126523490880484 76-77 0.1991316669272002 78-79 0.19671122630615548 80-81 0.1843064681233013 82-83 0.1986778343107543 84-85 0.18120527857758773 86-87 0.18503764289424185 88-89 0.19355961091417018 90-91 0.18952554321242898 92-93 0.19613132907403016 94-95 0.19378652722239312 96-97 0.18778585151605307 98-99 0.19509759922545902 100-101 0.17881005087967888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1983110.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.51640586021528 #Duplication Level Percentage of deduplicated Percentage of total 1 76.61764306744764 41.003008824506374 2 13.098421230738142 14.01960853424486 3 4.375230590048161 7.0243984796713965 4 1.904140836330619 4.076110952483166 5 1.0304353264155397 2.7572597570578714 6 0.6366402345586645 2.0442418307750487 7 0.4175986298875593 1.5643864434612715 8 0.29752753190136294 1.2738083321457192 9 0.21166332755205133 1.019471448869934 >10 1.2420374143886261 12.41815686421099 >50 0.10810548630274153 4.003890879650877 >100 0.056583380712601765 5.2710641903635835 >500 0.0022702535520744737 0.8259690183717102 >1k 0.0015135023680496482 1.9727375997136043 >5k 1.8918779600620602E-4 0.7258868444736424 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7504 0.37839555042332496 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6835 0.34466065926751416 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4792 0.24164065533429815 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4402 0.22197457528830977 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3716 0.18738244474587895 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3283 0.1655480533102047 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2854 0.14391536525961746 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2844 0.1434111067968998 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2578 0.12999783168861032 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2254 0.11365985749655844 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2134 0.10760875594394662 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.042584627176505E-5 0.0 4 0.0 0.0 0.0 5.042584627176505E-5 0.0 5 0.0 0.0 0.0 5.042584627176505E-5 0.0 6 0.0 0.0 0.0 5.042584627176505E-5 0.0 7 0.0 0.0 0.0 5.042584627176505E-5 0.0 8 0.0 0.0 0.0 5.042584627176505E-5 0.0 9 0.0 0.0 0.0 5.042584627176505E-5 0.0 10-11 0.0 0.0 0.0 5.042584627176505E-5 0.0 12-13 0.0 0.0 0.0 5.042584627176505E-5 0.0 14-15 0.0 0.0 0.0 5.042584627176505E-5 0.0 16-17 0.0 0.0 0.0 5.042584627176505E-5 0.0 18-19 0.0 0.0 0.0 7.563876940764758E-5 0.0 20-21 0.0 0.0 0.0 1.5127753881529518E-4 0.0 22-23 0.0 0.0 0.0 1.5127753881529518E-4 0.0 24-25 0.0 0.0 0.0 4.286196933100029E-4 0.0 26-27 0.0 0.0 0.0 5.546843089894156E-4 0.0 28-29 0.0 0.0 0.0 0.0012858590799300089 0.0 30-31 0.0 0.0 0.0 0.002924699083762373 0.0 32-33 0.0 0.0 0.0 0.004916520011497093 0.0 34-35 0.0 0.0 0.0 0.007236108939998286 0.0 36-37 0.0 0.0 0.0 0.010539001870798897 0.0 38-39 0.0 0.0 0.0 0.013614978493376565 0.0 40-41 0.0 0.0 0.0 0.018102878811563656 0.0 42-43 0.0 0.0 0.0 0.023876638209680754 0.0 44-45 0.0 0.0 0.0 0.029070500375672555 0.0 46-47 0.0 0.0 0.0 0.036155331776855544 0.0 48-49 0.0 0.0 0.0 0.04301324686981559 0.0 50-51 0.0 0.0 0.0 0.05458597858918567 0.0 52-53 0.0 0.0 0.0 0.08232019403865645 0.0 54-55 0.0 0.0 0.0 0.1167358341191361 0.0 56-57 0.0 0.0 0.0 0.14968912465773457 0.0 58-59 0.0 0.0 0.0 0.20898991987333027 0.0 60-61 0.0 0.0 0.0 0.28720040744083786 0.0 62-63 0.0 0.0 0.0 0.33835742848354355 0.0 64-65 0.0 0.0 0.0 0.383110367049735 0.0 66-67 0.0 0.0 0.0 0.5038550559474765 0.0 68-69 0.0 0.0 0.0 0.6272218888513497 0.0 70-71 0.0 0.0 0.0 0.6854637412952382 0.0 72-73 0.0 0.0 0.0 0.8017709557210644 0.0 74-75 0.0 0.0 0.0 0.9780345013640191 0.0 76-77 0.0 0.0 0.0 1.1548527313159633 0.0 78-79 0.0 0.0 0.0 1.3252164529451216 0.0 80-81 0.0 0.0 0.0 1.5475944350036055 0.0 82-83 0.0 0.0 0.0 1.79427767496508 0.0 84-85 0.0 0.0 0.0 2.0504913998719183 0.0 86-87 0.0 0.0 0.0 2.3831002818804805 0.0 88-89 0.0 0.0 0.0 2.7803550988094456 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9365 0.0 25.587461 1 TACATGG 9880 0.0 23.957693 2 ACATGGG 9645 0.0 23.949457 3 GAGTACT 6000 0.0 22.086613 12-13 ATGGGCG 240 5.8917067E-9 21.784273 5 CATGGGA 5895 0.0 20.479132 4 GTACTTT 6490 0.0 20.235067 14-15 CATGGGG 3645 0.0 20.211372 4 AGTACTT 6500 0.0 20.095345 12-13 ACTGCGT 195 1.0333959E-5 19.48689 8 AGAGTAC 11860 0.0 19.263317 10-11 GTATCAA 17505 0.0 19.121195 1 GTGCGTC 130 0.0075678625 18.268036 9 AGTACAT 7185 0.0 18.19192 2 GTACCTG 1705 0.0 18.125643 1 ATGGGAT 2615 0.0 17.993893 5 TACCTGG 1540 0.0 17.901106 2 CTATACG 135 0.0093693705 17.603453 4 GAGTACA 8140 0.0 17.464302 1 GTATAGG 665 0.0 17.159082 1 >>END_MODULE