FastQCFastQC Report
Thu 26 May 2016
SRR937237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983110
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88390.44571405519613133No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86360.435477608402963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67430.3400214814105118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46400.23397592670098985No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34700.17497768656302473No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34650.17472555733166592No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28310.14275557079536688No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25920.13070379353641504No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24480.12344247167328086No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22260.11224793380094902No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22220.11204623041586195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG93300.025.2746721
ACCGGTC1000.001654366723.7492628
TACATGG98150.023.6675592
ACATGGG97250.022.9584793
GAGTACT78850.022.95109612-13
TAGGACG1302.94066E-421.925164
GTACTTT83800.021.3681314-15
AGTACTT83100.020.94850712-13
GTATAGA5850.020.317471
CATGGGG33450.019.7402924
AGAGTAC138900.019.4234410-11
CAATTCG2450.019.38715478-79
GCGGTAT2251.6545255E-619.0171531
CGCGGAA2250.018.9994182-83
GTATCAA200200.018.8794161
ATTCGCG2550.018.62687180-81
CATGGGA61900.018.572044
AATTCGC2850.018.33276478-79
ACTTTTT100150.018.02240616-17
TACTTTT94750.017.99634714-15
Produced by FastQC (version 0.11.5)