##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937237_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1983110 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06595347711423 31.0 31.0 34.0 31.0 34.0 2 32.280004134919395 33.0 31.0 34.0 31.0 34.0 3 32.37164605089985 34.0 31.0 34.0 31.0 34.0 4 35.99825375294361 37.0 35.0 37.0 35.0 37.0 5 35.88770970848818 37.0 35.0 37.0 35.0 37.0 6 35.97376141515095 37.0 35.0 37.0 35.0 37.0 7 35.92495171725219 37.0 35.0 37.0 35.0 37.0 8 35.90407087856952 37.0 35.0 37.0 35.0 37.0 9 37.5839318040855 39.0 37.0 39.0 35.0 39.0 10-11 37.50702558103181 39.0 37.0 39.0 35.0 39.0 12-13 37.434265371058586 39.0 37.0 39.0 34.0 39.0 14-15 38.61070818058504 40.0 38.0 41.0 34.0 41.0 16-17 38.59497456016055 40.0 38.0 41.0 34.0 41.0 18-19 38.6064232442981 40.0 38.0 41.0 34.0 41.0 20-21 38.499812667981104 40.0 38.0 41.0 34.0 41.0 22-23 38.38031904432936 40.0 38.0 41.0 34.0 41.0 24-25 38.237006520061925 40.0 38.0 41.0 33.5 41.0 26-27 38.189459989612274 40.0 38.0 41.0 34.0 41.0 28-29 38.11258351780789 40.0 38.0 41.0 33.0 41.0 30-31 38.20469162073712 40.0 38.0 41.0 34.0 41.0 32-33 38.14734381854764 40.0 37.0 41.0 34.0 41.0 34-35 38.03367488439875 40.0 37.0 41.0 33.0 41.0 36-37 37.87869407143326 40.0 37.0 41.0 33.0 41.0 38-39 37.760414954288976 40.0 37.0 41.0 33.0 41.0 40-41 37.61706082869836 40.0 37.0 41.0 33.0 41.0 42-43 37.41532315403583 40.0 37.0 41.0 32.5 41.0 44-45 37.13887227637398 40.0 36.5 41.0 31.5 41.0 46-47 36.80788786300306 39.5 36.0 41.0 31.0 41.0 48-49 36.51589347035717 39.0 36.0 41.0 30.0 41.0 50-51 36.30567996732405 39.0 36.0 41.0 30.0 41.0 52-53 36.09513239305939 39.0 35.0 40.5 29.5 41.0 54-55 35.87813056260117 39.0 35.0 40.0 29.0 41.0 56-57 35.636115495358304 38.5 35.0 40.0 28.5 41.0 58-59 35.40139629168327 38.0 35.0 40.0 28.0 41.0 60-61 35.065253314238745 38.0 34.0 40.0 27.5 41.0 62-63 34.7149482378688 37.5 34.0 40.0 27.0 41.0 64-65 34.376647538462315 37.0 34.0 40.0 26.0 41.0 66-67 33.938901775494045 36.5 33.0 39.0 26.0 40.5 68-69 33.54585247414415 36.0 33.0 39.0 26.0 40.0 70-71 33.13700500728653 35.5 32.5 38.5 25.0 40.0 72-73 32.687806273983796 35.0 32.0 38.0 24.0 40.0 74-75 32.25188365748748 35.0 32.0 37.0 23.0 39.0 76-77 30.908899153350042 34.0 30.0 36.0 21.5 38.5 78-79 31.567813938712426 34.5 31.0 36.0 22.0 38.5 80-81 31.415560659771774 34.5 31.0 36.0 22.0 37.5 82-83 31.08402055357494 34.0 31.0 35.5 20.5 37.0 84-85 30.701464366575735 34.0 31.0 35.0 20.0 36.5 86-87 30.342376368431403 34.0 31.0 35.0 18.0 36.0 88-89 29.933225590108467 34.0 30.0 35.0 16.5 36.0 90-91 29.48243919903586 34.0 29.5 35.0 8.5 35.0 92-93 29.124137087705677 34.0 29.0 35.0 4.5 35.0 94-95 28.68711191008063 34.0 29.0 35.0 2.0 35.0 96-97 28.18633560417728 33.5 29.0 35.0 2.0 35.0 98-99 27.66107402009974 33.0 28.5 35.0 2.0 35.0 100-101 25.647769412690167 31.5 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23541381659367744 1101 2 0.20035876151714405 1101 3 0.15622921039908277 1101 4 0.1249249735038589 1101 5 0.08376105849264803 1101 6 0.1257220807282664 1101 7 0.105435330412746 1101 8 0.1348064555523436 1101 9 0.2483780148307133 1101 10-11 0.15624008564630998 1101 12-13 0.09327378663875407 1101 14-15 0.06092452628306688 1101 16-17 0.2003290783355709 1101 18-19 0.10615503443791141 1101 20-21 0.12727276712914204 1101 22-23 0.05281710523340877 1101 24-25 0.14975712730529267 1101 26-27 0.002615114199855384 1101 28-29 0.053653335258083246 1101 30-31 0.15989618089958668 1101 32-33 0.11857530467938915 1101 34-35 0.08991638385925427 1101 36-37 0.09475306772385039 1101 38-39 0.04460825093195098 1101 40-41 0.04115036641715619 1101 42-43 0.38430865184439256 1101 44-45 0.26981531418717 1101 46-47 0.12660012691074485 1101 48-49 0.22552622663951638 1101 50-51 0.24717749723705396 1101 52-53 0.17990493591943135 1101 54-55 0.29774430960001297 1101 56-57 0.38776841253513084 1101 58-59 0.2727501108100796 1101 60-61 0.13607380624836907 1101 62-63 0.16936386577832963 1101 64-65 0.1699240579211292 1101 66-67 0.305941944014414 1101 68-69 0.48368743588687124 1101 70-71 0.11971560510119161 1101 72-73 0.2068264793631016 1101 74-75 0.034980801437008324 1101 76-77 0.11015507573023342 1101 78-79 0.08761397230277623 1101 80-81 0.18721445018235983 1101 82-83 0.1925726867054678 1101 84-85 0.15204029655450668 1101 86-87 0.29558075044930376 1101 88-89 0.27115732974451845 1101 90-91 0.2938571402137633 1101 92-93 0.19205013404836535 1101 94-95 0.13950217604345738 1101 96-97 -0.008787094994872291 1101 98-99 0.11871989786372339 1101 100-101 0.5147559397978654 1102 1 0.19396766110381947 1102 2 0.21407633956631145 1102 3 0.19310883338132356 1102 4 0.18940835259198252 1102 5 0.04214838769393481 1102 6 0.16419206823309906 1102 7 0.16974307363519614 1102 8 0.19505945979861394 1102 9 0.17346202167373548 1102 10-11 0.16079567348574386 1102 12-13 0.23946992812204115 1102 14-15 0.16631334213511906 1102 16-17 0.0635575990985231 1102 18-19 0.1378965265870633 1102 20-21 0.12694012739243732 1102 22-23 0.18462582891570634 1102 24-25 0.24805171347306043 1102 26-27 0.20832497365206848 1102 28-29 0.2101223507721528 1102 30-31 0.17495109316634228 1102 32-33 0.16174485452778953 1102 34-35 0.15219558495059715 1102 36-37 0.20332730319317704 1102 38-39 0.10670634382352517 1102 40-41 0.17261237864651946 1102 42-43 0.15460742825654705 1102 44-45 0.20929937731706616 1102 46-47 0.1802777308622936 1102 48-49 0.054399824842505495 1102 50-51 0.1844929525417669 1102 52-53 0.1554068597054581 1102 54-55 -4.969864571648941E-4 1102 56-57 -0.07323729823637137 1102 58-59 -0.09541406442448164 1102 60-61 0.0195626016443029 1102 62-63 -0.08257053917700574 1102 64-65 -0.12503279533041933 1102 66-67 0.05152700819056122 1102 68-69 -0.0399026237485387 1102 70-71 0.05369267602134187 1102 72-73 0.1785407661941889 1102 74-75 0.008754509998730953 1102 76-77 -0.01573961832069415 1102 78-79 0.0539571632207867 1102 80-81 0.08665171182638076 1102 82-83 0.22641477338463645 1102 84-85 0.09877650529538684 1102 86-87 0.22236248379810064 1102 88-89 0.19968069856550485 1102 90-91 0.16478288976066224 1102 92-93 0.2042349373765866 1102 94-95 0.35111642339556326 1102 96-97 0.30281516533969466 1102 98-99 0.3690309739982247 1102 100-101 0.694403026366281 1103 1 0.12172856058707993 1103 2 0.10368190680684108 1103 3 0.031641979755086425 1103 4 0.06792747699799406 1103 5 -0.05349441566095692 1103 6 -0.027159753324376368 1103 7 0.04772339094713374 1103 8 0.061423615494391015 1103 9 0.16058907628151076 1103 10-11 0.02557586615796481 1103 12-13 0.033317789771963646 1103 14-15 -0.010158964478570454 1103 16-17 0.11062207418355996 1103 18-19 0.15915538769168336 1103 20-21 0.10355395201397499 1103 22-23 0.11489185680384395 1103 24-25 0.04733503512142079 1103 26-27 0.10241237725104213 1103 28-29 -0.07397699746601916 1103 30-31 -0.12069495601441105 1103 32-33 -0.11696317694373448 1103 34-35 0.02228594874275558 1103 36-37 -0.011708660557438577 1103 38-39 0.03447178746299784 1103 40-41 0.01208551619163245 1103 42-43 -0.03213467140329129 1103 44-45 -0.23565141596277783 1103 46-47 -0.1917588301202855 1103 48-49 -0.20908982746462357 1103 50-51 -0.21886777556189685 1103 52-53 -0.19198284713154834 1103 54-55 -0.3081168872647311 1103 56-57 -0.24049470521099892 1103 58-59 -0.15937371231729003 1103 60-61 -0.2181415608027848 1103 62-63 -0.2640310309845404 1103 64-65 -0.37689525021344394 1103 66-67 -0.28580842843793164 1103 68-69 -0.21841071234660347 1103 70-71 -0.27492901648209767 1103 72-73 -0.1685503246629665 1103 74-75 -0.23850276714286167 1103 76-77 -0.0964276035716587 1103 78-79 -0.17223924819413128 1103 80-81 -0.2714226046026944 1103 82-83 -0.34749333079339095 1103 84-85 -0.11000540117289503 1103 86-87 -0.23391299593496484 1103 88-89 -0.36135251272477475 1103 90-91 -0.35632601418885557 1103 92-93 -0.45121999757706277 1103 94-95 -0.23108995919642084 1103 96-97 -0.17483083190784754 1103 98-99 -0.009797153030415728 1103 100-101 0.3540976590686249 1104 1 0.18891875521629942 1104 2 0.25038060106651017 1104 3 0.1936430884025384 1104 4 0.10694262851314562 1104 5 0.02981428106237871 1104 6 0.11734012349257483 1104 7 0.08774864347238776 1104 8 0.16082925727808117 1104 9 0.1656334221795177 1104 10-11 0.16404901225305935 1104 12-13 0.13882869147189325 1104 14-15 0.05781960167072242 1104 16-17 0.059584256987207596 1104 18-19 0.155247405430039 1104 20-21 0.0036386403608617 1104 22-23 0.18402064468264 1104 24-25 0.17465395603790057 1104 26-27 0.195959125218522 1104 28-29 0.05358367018611432 1104 30-31 0.13084945147512173 1104 32-33 0.061833940572888935 1104 34-35 0.14182124068658197 1104 36-37 0.06114179521984653 1104 38-39 -0.034670858508910385 1104 40-41 0.22064673816748837 1104 42-43 0.20136649883568225 1104 44-45 0.18649073727693377 1104 46-47 0.1238238257062747 1104 48-49 0.17247736888916165 1104 50-51 0.08696032249181229 1104 52-53 0.05785855469155621 1104 54-55 0.06592998388579474 1104 56-57 0.03917701196071022 1104 58-59 0.05236871192513348 1104 60-61 0.19982468704151302 1104 62-63 0.10578296260994335 1104 64-65 -0.0020045751703250403 1104 66-67 0.036729450349945125 1104 68-69 0.12322515855755256 1104 70-71 0.014458763759343185 1104 72-73 0.03404267853979093 1104 74-75 0.03418139151690269 1104 76-77 0.07027909758626194 1104 78-79 0.1258360166494228 1104 80-81 0.12975995267742846 1104 82-83 0.19679405526228777 1104 84-85 0.22530194053688746 1104 86-87 0.19326426447400635 1104 88-89 0.4525486944691117 1104 90-91 0.3776124558776637 1104 92-93 0.28633543479544343 1104 94-95 0.37660744164122306 1104 96-97 0.4411136741281254 1104 98-99 0.6928574416973454 1104 100-101 1.130247880798116 1105 1 0.13543137823727136 1105 2 0.19696008330870285 1105 3 0.14163998613467754 1105 4 0.13654789167104298 1105 5 0.07938370767769953 1105 6 0.12738237852038736 1105 7 0.11132320487589453 1105 8 0.07030187174106572 1105 9 0.2181847700614199 1105 10-11 0.145749643404109 1105 12-13 0.07465052501917313 1105 14-15 0.1480996080892112 1105 16-17 0.2083460242101367 1105 18-19 0.27977939088147963 1105 20-21 0.2015619929496495 1105 22-23 0.2029351183668453 1105 24-25 0.1272841978008472 1105 26-27 0.18289483339139423 1105 28-29 0.1817862268827355 1105 30-31 0.1194432683343365 1105 32-33 0.14720648015354953 1105 34-35 0.15835552609522807 1105 36-37 0.12606878495027019 1105 38-39 0.027100375979749458 1105 40-41 0.1125955507477201 1105 42-43 0.07882691817671628 1105 44-45 0.06894554044338719 1105 46-47 -0.08464991199162597 1105 48-49 0.00884611086605247 1105 50-51 0.08886581029668861 1105 52-53 0.2734461135276689 1105 54-55 0.18098133176769693 1105 56-57 0.10853008213615567 1105 58-59 0.07703976455670869 1105 60-61 0.01801440676765509 1105 62-63 0.11662752829244027 1105 64-65 0.06839149885620799 1105 66-67 0.17927330999906843 1105 68-69 0.29195777516003574 1105 70-71 0.280277655500889 1105 72-73 0.2824581149967855 1105 74-75 0.3187074933568752 1105 76-77 0.3314913889854907 1105 78-79 0.3984611771115567 1105 80-81 0.25508756286142287 1105 82-83 0.3219411455489798 1105 84-85 0.22442608473885528 1105 86-87 0.2402662969943279 1105 88-89 0.3888385425649652 1105 90-91 0.3937188422704736 1105 92-93 0.21194786526185183 1105 94-95 0.030878408692995407 1105 96-97 0.12222193257913005 1105 98-99 0.31597869758095953 1105 100-101 0.7222595410462986 1106 1 -0.1255038991453752 1106 2 -0.0157147625898304 1106 3 0.019766467879577476 1106 4 0.047821006891524576 1106 5 -0.07760716477370266 1106 6 -0.0883152269798515 1106 7 0.017365760589505896 1106 8 0.03705151612228974 1106 9 -0.04787734468490612 1106 10-11 -0.19499982316772702 1106 12-13 -0.24822909802492177 1106 14-15 -0.09698840755801541 1106 16-17 0.0644389592504453 1106 18-19 -0.018347683561387385 1106 20-21 -0.04806016210013553 1106 22-23 -0.015585211173224423 1106 24-25 0.09270768049407252 1106 26-27 -0.014873658784992472 1106 28-29 -0.013633699622715767 1106 30-31 0.03677672020726419 1106 32-33 -0.0244314406619921 1106 34-35 -0.008585263378456887 1106 36-37 -0.11149196659074079 1106 38-39 0.048978339469549326 1106 40-41 -0.025569752713636262 1106 42-43 -0.37953605005466784 1106 44-45 -0.23244878151014348 1106 46-47 -0.030647719009174068 1106 48-49 0.16180663718184007 1106 50-51 0.014119188675074668 1106 52-53 0.10401599261728478 1106 54-55 0.021056604408755675 1106 56-57 -0.029235150201451177 1106 58-59 0.05321330651973 1106 60-61 0.10307397291274611 1106 62-63 0.04959338888988185 1106 64-65 -0.035767759085949535 1106 66-67 0.0119546755751756 1106 68-69 0.09677492783902863 1106 70-71 0.1294952941922034 1106 72-73 0.19572417711152923 1106 74-75 0.017928095538003674 1106 76-77 0.1258918749978193 1106 78-79 -0.005343943798827411 1106 80-81 0.12748710055701196 1106 82-83 -0.02982509437515546 1106 84-85 -0.07591619833369023 1106 86-87 0.16622350420153964 1106 88-89 -0.012040132162393746 1106 90-91 0.06915003003718923 1106 92-93 0.07054494918788379 1106 94-95 -0.35630153440799717 1106 96-97 0.07406522323820752 1106 98-99 0.2252885008052381 1106 100-101 0.7217112954322644 1107 1 0.10289352933234852 1107 2 0.05223380696473612 1107 3 0.020383787554713706 1107 4 0.03489587619337442 1107 5 -0.04713424002883215 1107 6 0.03770841443458295 1107 7 0.024088757676523187 1107 8 -0.023399995346352398 1107 9 0.05262398641064436 1107 10-11 0.0393917552415175 1107 12-13 -0.0035179119995021324 1107 14-15 0.010967179185044529 1107 16-17 -0.0040081682839385735 1107 18-19 -0.08715893324789903 1107 20-21 0.07237384256833934 1107 22-23 0.07523674032859873 1107 24-25 0.0916394280874897 1107 26-27 0.015474475752114358 1107 28-29 0.02989577739177207 1107 30-31 -0.03332824825155711 1107 32-33 -0.07709230548828572 1107 34-35 -0.09765110920286446 1107 36-37 -0.10015414322133864 1107 38-39 -0.04583448727581896 1107 40-41 0.024943755977062665 1107 42-43 -0.00521666911055263 1107 44-45 -0.0032778948847607126 1107 46-47 0.060894036879481916 1107 48-49 0.022151926261074095 1107 50-51 -0.004136955584819191 1107 52-53 -0.12305697167462881 1107 54-55 -0.17839190727208631 1107 56-57 -0.1197719530642658 1107 58-59 0.033230596293442716 1107 60-61 0.061767811290742713 1107 62-63 0.035706710455556845 1107 64-65 -0.07684604293725528 1107 66-67 0.01872891501803764 1107 68-69 0.0676355100486532 1107 70-71 -0.07008885901881712 1107 72-73 0.03776388812875808 1107 74-75 -0.038915351227821304 1107 76-77 -0.051244509245094605 1107 78-79 -0.08549060544977038 1107 80-81 -0.11764608134008725 1107 82-83 0.056845020600633234 1107 84-85 0.10801311374069655 1107 86-87 0.12452307786513472 1107 88-89 0.1129894075486213 1107 90-91 0.1690925180283891 1107 92-93 0.21888252659398333 1107 94-95 0.19843494725568078 1107 96-97 0.31883548868567146 1107 98-99 0.6296708676474516 1107 100-101 0.9406397394008579 1108 1 0.021254203759134782 1108 2 0.032706964008696104 1108 3 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-0.54893983621686 2316 74-75 -0.42936688780150334 2316 76-77 -0.37068732406425653 2316 78-79 -0.45407126701245204 2316 80-81 -0.6781473866699379 2316 82-83 -0.6513780685822397 2316 84-85 -0.7170761082435995 2316 86-87 -0.933791922183751 2316 88-89 -0.9324746934359993 2316 90-91 -0.9596780274723784 2316 92-93 -0.941703600731902 2316 94-95 -0.9794226006797473 2316 96-97 -0.8783142966926789 2316 98-99 -0.8702648436350948 2316 100-101 -1.4125067410974168 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 16.0 5 36.0 6 81.0 7 167.0 8 413.0 9 873.0 10 1564.0 11 2331.0 12 3607.0 13 5201.0 14 6873.0 15 9456.0 16 13215.0 17 18149.0 18 15919.0 19 9467.0 20 8266.0 21 8436.0 22 8770.0 23 9811.0 24 11390.0 25 14079.0 26 17487.0 27 21943.0 28 27859.0 29 35530.0 30 45343.0 31 57267.0 32 74053.0 33 96769.0 34 132613.0 35 189561.0 36 289232.0 37 441710.0 38 370505.0 39 35108.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70804636673801 20.23019892650527 15.365667341763467 21.696087364993254 2 15.419013569595233 21.38494586785403 39.598458986137935 23.597581576412807 3 19.793556585363394 25.340097120179923 30.05057712381058 24.815769170646107 4 12.779876053269865 17.260868030517724 38.123200427611174 31.83605548860124 5 13.181263172865826 38.24545370640756 34.34984105561465 14.223442065111971 6 31.05274039261564 37.42959291214305 17.340893848551016 14.1767728466903 7 28.135706037486575 33.40384547503669 20.76390114517097 17.69654734230577 8 26.32688050587209 34.841234223013345 19.60178709199187 19.23009817912269 9 26.108788720746706 17.252144359112705 20.73566267125878 35.90340424888181 10-11 24.74668576125379 27.26104452097967 27.276928662555278 20.71534105521126 12-13 26.16955496096441 24.458488942857056 27.994813071905973 21.377143024272563 14-15 22.633338544004115 25.91079163536062 26.90927381738784 24.546596003247426 16-17 21.505161085365916 29.46765434090898 27.28434126195723 21.742843311767878 18-19 21.38983717494239 27.94802103766306 29.92277281643479 20.739368970959752 20-21 22.4459308863351 26.507858868141454 29.90103927669166 21.145170968831785 22-23 21.861778075516863 26.566876448336902 29.96464866633873 21.606696809807506 24-25 22.05995996684999 26.85455874407149 29.942365677284606 21.14311561179392 26-27 21.90956177699583 26.81935274194772 30.140888820645173 21.130196660411276 28-29 21.258176823106297 27.412629270959194 30.24463904986475 21.084554856069758 30-31 21.63757935767557 26.770199333370314 29.986057253505855 21.60616405544826 32-33 21.524751526642497 27.006595700692348 29.95645728174433 21.512195490920828 34-35 22.23368849937724 26.815305252860405 29.761258830826325 21.189747416936026 36-37 22.09584944859337 26.57807685907489 29.967349264539035 21.358724427792712 38-39 21.624897257338223 27.092723046124522 30.058014936135663 21.224364760401592 40-41 21.85814200926827 26.986274084644823 29.90192172900142 21.253662177085488 42-43 21.566422767569296 27.137857498945333 29.405092806813204 21.890626926672166 44-45 21.93297396513557 26.892482010579343 29.2700858752162 21.904458149068887 46-47 22.231822743065184 27.407733307784238 28.852232100085217 21.508211849065358 48-49 22.19506230113307 27.446687274029173 28.723041081936955 21.635209342900797 50-51 21.777032035540138 27.622723903363912 28.890354039866672 21.70989002122928 52-53 22.31507581525987 27.931052740392616 28.116594641749575 21.637276802597942 54-55 21.928687768202472 27.452309755888475 28.438008985885805 22.180993490023244 56-57 22.154419069038024 27.71744885558542 28.27889022797525 21.849241847401306 58-59 22.620606017820492 27.276903449632144 28.351906853376768 21.750583679170596 60-61 22.206231626082264 27.559868085986157 28.978826187150485 21.255074100781098 62-63 21.985845464951513 27.525755000983303 28.86095577149024 21.627443762574945 64-65 21.757693723494913 27.756554099369176 28.58373968161121 21.902012495524705 66-67 21.907206357690697 27.68436950043114 28.083212731517666 22.32521141036049 68-69 21.81374205162598 27.574365516789285 28.495242321404255 22.116650110180476 70-71 22.191532491894044 27.53974817332372 28.38317087806526 21.885548456716975 72-73 22.14368376863555 27.217494787214996 28.671326026609783 21.967495417539666 74-75 21.74687737946962 28.134773159330546 28.10000453832616 22.018344922873666 76-77 21.97215484768873 27.607066678096526 28.248559081442785 22.172219392771957 78-79 22.011814775781474 27.507500844632926 28.236860285107735 22.243824094477866 80-81 22.10454790707525 27.818754380745396 28.062134727776066 22.014562984403284 82-83 22.080822225544733 27.586252975446847 28.399346378697455 21.933578420310972 84-85 21.874957452655835 27.64926868994521 28.186562855349116 22.289211002049836 86-87 21.864546091744785 27.606940613480845 28.15262390891075 22.37588938586362 88-89 21.89359137919732 27.5731805094019 28.00860769195859 22.52462041944219 90-91 21.916056263731388 27.406316419063586 28.21280727859009 22.464820038614935 92-93 22.07983238300785 27.299457791303226 28.286224671819394 22.334485153869522 94-95 21.76954379736878 27.150964898568407 28.435638971111032 22.643852332951777 96-97 21.569227123054194 27.08349511625679 28.78317390361604 22.564103857072983 98-99 21.61785944083731 27.266038965099227 28.11498415618312 23.001117437880335 100-101 21.081034056695596 27.1018523655106 28.626738011064766 23.190375566729035 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 267.0 1 193.5 2 154.5 3 241.0 4 230.5 5 209.5 6 419.5 7 971.0 8 1625.5 9 2018.0 10 1966.0 11 1868.5 12 2028.0 13 2228.0 14 2410.5 15 2558.0 16 2720.5 17 2898.0 18 3129.0 19 3381.5 20 3675.0 21 4214.0 22 5013.5 23 5941.0 24 6984.5 25 8468.0 26 10642.5 27 13324.0 28 15830.5 29 19138.5 30 23404.5 31 27536.0 32 32300.0 33 37819.0 34 43908.5 35 48566.5 36 54065.5 37 60399.0 38 63882.5 39 65002.5 40 65205.0 41 67354.0 42 69598.0 43 71818.5 44 73926.5 45 74270.5 46 73338.0 47 78801.0 48 94355.0 49 92796.0 50 76723.0 51 68359.0 52 63282.0 53 59953.0 54 56289.5 55 50661.5 56 44871.5 57 39376.5 58 34954.0 59 30641.0 60 25917.0 61 21235.0 62 16834.0 63 12978.5 64 9789.5 65 7548.5 66 5465.0 67 4284.5 68 3262.5 69 2059.0 70 1397.0 71 1083.0 72 862.0 73 716.0 74 547.5 75 424.0 76 295.5 77 152.0 78 74.0 79 42.0 80 29.5 81 17.5 82 8.0 83 4.5 84 4.5 85 3.0 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05909909183050865 2 0.0 3 0.0 4 0.0 5 0.017195213578671886 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002420440621044723 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.320264634841235E-4 24-25 1.764904619511777E-4 26-27 0.0010841556948429486 28-29 0.003328105853936494 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012833377876164207 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.521292313588253E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.2691630822294277E-4 84-85 0.0012606461567941263 86-87 0.0 88-89 0.0 90-91 0.010009530484945363 92-93 8.824523097558884E-4 94-95 0.0 96-97 0.0 98-99 1.008516925435301E-4 100-101 0.012102203105223613 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1983110.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.732858379398884 #Duplication Level Percentage of deduplicated Percentage of total 1 75.27139684962988 38.94004513241434 2 13.814780939716155 14.293562117935096 3 4.618023677342433 7.167106946780007 4 2.0237602688325658 4.187796135254774 5 1.112878168050978 2.878618433065306 6 0.669869102034858 2.079254603298265 7 0.4511652238776664 1.6338046642801176 8 0.31567822078399604 1.3064749351423266 9 0.22329057390979498 1.0396313673777111 >10 1.3173830214237572 12.789680587470562 >50 0.1151144404023266 4.100333732953226 >100 0.06245145232356988 5.7484613070541375 >500 0.0024465474837353754 0.8815131067756896 >1k 0.001467928490241225 1.7276381098507012 >5k 2.93585698048245E-4 1.2260788203476711 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8839 0.44571405519613133 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8636 0.435477608402963 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6743 0.3400214814105118 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4640 0.23397592670098985 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3470 0.17497768656302473 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3465 0.17472555733166592 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2831 0.14275557079536688 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2592 0.13070379353641504 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2448 0.12344247167328086 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2226 0.11224793380094902 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2222 0.11204623041586195 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.042584627176505E-5 0.0 12-13 0.0 0.0 0.0 5.042584627176505E-5 0.0 14-15 0.0 0.0 0.0 5.042584627176505E-5 0.0 16-17 0.0 0.0 0.0 7.563876940764758E-5 0.0 18-19 0.0 0.0 0.0 1.2606461567941265E-4 0.0 20-21 0.0 0.0 0.0 2.017033850870602E-4 0.0 22-23 0.0 0.0 0.0 2.017033850870602E-4 0.0 24-25 0.0 0.0 0.0 4.538326164458855E-4 0.0 26-27 0.0 0.0 0.0 5.546843089894156E-4 0.0 28-29 0.0 0.0 0.0 0.0013614978493376566 0.0 30-31 0.0 0.0 0.0 0.003000337853170021 0.0 32-33 0.0 0.0 0.0 0.00499215878090474 0.0 34-35 0.0 0.0 0.0 0.007336960632541816 0.0 36-37 0.0 0.0 0.0 0.01066506648647831 0.0 38-39 0.0 0.0 0.0 0.013816681878463626 0.0 40-41 0.0 0.0 0.0 0.018405433889194245 0.0 42-43 0.0 0.0 0.0 0.024305257902990755 0.0 44-45 0.0 0.0 0.0 0.02944869422271079 0.0 46-47 0.0 0.0 0.0 0.03681086777838849 0.0 48-49 0.0 0.0 0.0 0.04374442164075619 0.0 50-51 0.0 0.0 0.0 0.05551885674521333 0.0 52-53 0.0 0.0 0.0 0.0836816918879941 0.0 54-55 0.0 0.0 0.0 0.11834946119983258 0.0 56-57 0.0 0.0 0.0 0.1516557326623334 0.0 58-59 0.0 0.0 0.0 0.21130950880183147 0.0 60-61 0.0 0.0 0.0 0.28997382898578494 0.0 62-63 0.0 0.0 0.0 0.3418620247994312 0.0 64-65 0.0 0.0 0.0 0.38684187967384565 0.0 66-67 0.0 0.0 0.0 0.5086202984201582 0.0 68-69 0.0 0.0 0.0 0.6331217128651462 0.0 70-71 0.0 0.0 0.0 0.6921451659262472 0.0 72-73 0.0 0.0 0.0 0.8087549354297039 0.0 74-75 0.0 0.0 0.0 0.9871867924623445 0.0 76-77 0.0 0.0 0.0 1.1670053602674586 0.0 78-79 0.0 0.0 0.0 1.339260051131808 0.0 80-81 0.0 0.0 0.0 1.5649661390442287 0.0 82-83 0.0 0.0 0.0 1.814271523011835 0.0 84-85 0.0 0.0 0.0 2.072628346385223 0.0 86-87 0.0 0.0 0.0 2.4098007674813804 0.0 88-89 0.0 0.0 0.0 2.8115939105748042 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9330 0.0 25.274672 1 ACCGGTC 100 0.0016543667 23.749262 8 TACATGG 9815 0.0 23.667559 2 ACATGGG 9725 0.0 22.958479 3 GAGTACT 7885 0.0 22.951096 12-13 TAGGACG 130 2.94066E-4 21.92516 4 GTACTTT 8380 0.0 21.36813 14-15 AGTACTT 8310 0.0 20.948507 12-13 GTATAGA 585 0.0 20.31747 1 CATGGGG 3345 0.0 19.740292 4 AGAGTAC 13890 0.0 19.42344 10-11 CAATTCG 245 0.0 19.387154 78-79 GCGGTAT 225 1.6545255E-6 19.017153 1 CGCGGAA 225 0.0 18.99941 82-83 GTATCAA 20020 0.0 18.879416 1 ATTCGCG 255 0.0 18.626871 80-81 CATGGGA 6190 0.0 18.57204 4 AATTCGC 285 0.0 18.332764 78-79 ACTTTTT 10015 0.0 18.022406 16-17 TACTTTT 9475 0.0 17.996347 14-15 >>END_MODULE