FastQCFastQC Report
Thu 26 May 2016
SRR937236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983143
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76220.38433940467228034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68020.34299089879045536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48570.24491425983905346No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44690.22534935705594605No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38340.19332947750111817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31310.1578806974585292No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30200.15228352166233095No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28940.14592997075853834No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25060.1263650679754309No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22520.11355711615349978No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21530.108565040443377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95100.026.6809981
TACATGG99000.024.8096032
ACATGGG98400.023.7532733
GAGTACT61000.021.41444812-13
TCGCGCT1900.021.24245660-61
CATGGGG35600.020.68564
GTACTTT64200.020.42027914-15
AGTACTT65300.019.7497112-13
CATGGGA62400.019.4913444
CGCGCTT2350.019.19531262-63
GTATCAA173400.018.6885911
AGAGTAC120400.018.64120110-11
TCGCGGA1951.4551915E-1118.266982-83
CTCGCGC2350.018.18503260-61
CGCGGAA1851.4188117E-1017.9706882-83
AGTACAT72150.017.3174462
GTGTAGC9650.017.2338391
TACTTTT73850.017.10876714-15
GAGTACA82500.016.9906271
ATTCGCG2104.7293724E-1116.96190880-81
Produced by FastQC (version 0.11.5)