##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937236_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1983143 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.704839237513383 33.0 31.0 34.0 30.0 34.0 2 31.798000446765563 33.0 31.0 34.0 30.0 34.0 3 31.84714566725647 33.0 31.0 34.0 30.0 34.0 4 35.453673789535095 37.0 35.0 37.0 33.0 37.0 5 35.47791258623307 37.0 35.0 37.0 33.0 37.0 6 35.51284198870177 37.0 35.0 37.0 33.0 37.0 7 35.4029497620696 37.0 35.0 37.0 33.0 37.0 8 35.4091409444503 37.0 35.0 37.0 33.0 37.0 9 37.022451734443756 39.0 37.0 39.0 33.0 39.0 10-11 36.91104902672173 39.0 37.0 39.0 33.0 39.0 12-13 36.77215284021374 39.0 37.0 39.0 33.0 39.0 14-15 37.9258727182054 40.0 38.0 41.0 33.0 41.0 16-17 37.8633336073092 40.0 38.0 41.0 33.0 41.0 18-19 37.82987157254923 40.0 38.0 41.0 33.0 41.0 20-21 37.71739834192491 40.0 37.5 41.0 32.0 41.0 22-23 37.56304563009324 40.0 37.0 41.0 32.0 41.0 24-25 37.496980550570484 40.0 37.0 41.0 32.0 41.0 26-27 37.53795137314859 40.0 37.0 41.0 32.0 41.0 28-29 37.47609526897455 40.0 37.0 41.0 32.0 41.0 30-31 37.291752284126765 40.0 37.0 41.0 31.5 41.0 32-33 37.086492249928526 39.0 36.0 41.0 31.0 41.0 34-35 37.107449387159676 39.5 36.0 41.0 31.0 41.0 36-37 36.97224708455215 39.5 36.0 41.0 30.5 41.0 38-39 36.873329860731175 39.0 36.0 41.0 30.5 41.0 40-41 36.68659244441778 39.0 36.0 41.0 30.0 41.0 42-43 36.434344875785555 39.0 35.5 41.0 30.0 41.0 44-45 36.22019314794747 39.0 35.0 41.0 30.0 41.0 46-47 35.91459819085159 39.0 35.0 41.0 28.5 41.0 48-49 35.614612763678664 39.0 35.0 40.0 27.0 41.0 50-51 34.64884983079889 38.0 34.0 39.5 25.5 40.5 52-53 34.787280342365634 38.0 34.5 39.5 26.5 40.5 54-55 35.17595075090399 39.0 35.0 40.0 26.5 41.0 56-57 34.963257818523424 38.0 34.5 40.0 26.0 41.0 58-59 34.700828936692915 38.0 34.0 40.0 25.5 41.0 60-61 34.37891291752536 38.0 34.0 40.0 24.5 41.0 62-63 34.081118204789064 37.5 34.0 40.0 23.5 41.0 64-65 33.66949408086053 37.0 33.0 40.0 22.5 41.0 66-67 33.304075399504725 36.5 33.0 39.0 22.0 41.0 68-69 32.82227781859402 36.0 32.0 39.0 20.5 40.5 70-71 32.34188482625812 35.5 31.5 38.5 19.5 40.0 72-73 31.867104893595673 35.0 31.0 38.0 18.0 40.0 74-75 31.38138475137698 35.0 31.0 37.0 15.0 39.0 76-77 30.893948646164194 35.0 30.5 36.5 10.5 39.0 78-79 30.382562931669575 34.0 30.0 36.0 7.5 38.5 80-81 29.868554360426856 34.0 29.0 36.0 4.5 37.5 82-83 29.374678477548013 34.0 29.0 35.0 2.0 37.0 84-85 28.833761861852622 34.0 29.0 35.0 2.0 36.5 86-87 28.379011246289352 34.0 27.5 35.0 2.0 36.0 88-89 27.896650418048523 33.0 27.0 35.0 2.0 36.0 90-91 27.362313509414097 33.0 26.0 35.0 2.0 35.0 92-93 26.83182377670193 33.0 25.0 35.0 2.0 35.0 94-95 26.260651148202626 32.0 24.5 35.0 2.0 35.0 96-97 25.487641839242052 32.0 21.5 34.0 2.0 35.0 98-99 24.541587520415824 32.0 10.0 34.0 2.0 35.0 100-101 21.901026048045956 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.0384891775225249 1101 2 0.007894739441894671 1101 3 0.03849276696791293 1101 4 -0.1428744443024712 1101 5 -0.08548874212064561 1101 6 -0.12126939089320388 1101 7 -0.12930286801201163 1101 8 -0.2520516519636544 1101 9 -0.21112393899979054 1101 10-11 -0.2347572928934767 1101 12-13 -0.0369291525254809 1101 14-15 -0.2374093454746813 1101 16-17 -0.21026793324779192 1101 18-19 -0.010152516307059045 1101 20-21 0.06242611681720689 1101 22-23 -0.0985320817914257 1101 24-25 -0.05928229269024854 1101 26-27 -0.17545112906371685 1101 28-29 -0.13911414368470787 1101 30-31 -0.06498292458788058 1101 32-33 -0.3538639837032065 1101 34-35 -0.18157677723847598 1101 36-37 -0.20413881389546873 1101 38-39 -0.17181463943871478 1101 40-41 -0.34159125297446025 1101 42-43 -0.37369444137132746 1101 44-45 -0.33941571392193026 1101 46-47 -0.16138877615176028 1101 48-49 -0.09177451435102313 1101 50-51 -0.22882046037994286 1101 52-53 -0.10426937994644447 1101 54-55 -0.13258044714111605 1101 56-57 -0.28315867376731774 1101 58-59 -0.13468071730127917 1101 60-61 0.008261077122604377 1101 62-63 -0.18109289549791896 1101 64-65 -0.3827007154853419 1101 66-67 -0.18848420740079064 1101 68-69 -0.20240702784386144 1101 70-71 -0.3315265906474494 1101 72-73 -0.2230386772084536 1101 74-75 -0.2439050241710099 1101 76-77 -0.1325490981864057 1101 78-79 -0.28258817034655337 1101 80-81 -0.13511409653475326 1101 82-83 -0.11864792919528355 1101 84-85 -0.270531886000434 1101 86-87 -0.32194180505953796 1101 88-89 -0.31971116863768856 1101 90-91 -0.3725802617194347 1101 92-93 -0.16801853995405125 1101 94-95 -0.09630573601322112 1101 96-97 0.006276276660088342 1101 98-99 -0.18059914164494728 1101 100-101 0.22853845599285805 1102 1 0.006001731026490376 1102 2 0.07583817550827732 1102 3 0.02714077615679855 1102 4 0.06037628532916983 1102 5 0.07000590571888665 1102 6 0.01224139252369838 1102 7 -0.02232614515281739 1102 8 0.020395668975680792 1102 9 0.009558166282680247 1102 10-11 0.023710446268658814 1102 12-13 -0.07141602603383745 1102 14-15 0.06300355840193816 1102 16-17 0.09722119392247919 1102 18-19 0.08628002629586717 1102 20-21 0.0887062678561108 1102 22-23 0.11713602694374714 1102 24-25 0.13079643645534844 1102 26-27 0.08259109671973164 1102 28-29 0.22453804956623458 1102 30-31 0.15404894077014575 1102 32-33 -0.030103555071839594 1102 34-35 0.22375108127832277 1102 36-37 0.15587037957785554 1102 38-39 0.2160283103594196 1102 40-41 0.16831545963665207 1102 42-43 0.1999964057350212 1102 44-45 0.1837878332456384 1102 46-47 0.3457295170260437 1102 48-49 0.3656139660664195 1102 50-51 0.2769668425392737 1102 52-53 0.2161405893551489 1102 54-55 0.25028988820285747 1102 56-57 0.24283145872416156 1102 58-59 0.0828660926093292 1102 60-61 0.2619852362407613 1102 62-63 0.19497919875695402 1102 64-65 0.31587956921429594 1102 66-67 0.12050522125120722 1102 68-69 0.10127070538034388 1102 70-71 -0.12380716811679093 1102 72-73 0.027285960153733413 1102 74-75 0.03007859264685564 1102 76-77 0.1070572331592139 1102 78-79 -0.08350637136035743 1102 80-81 -0.06969955342286482 1102 82-83 -4.378594762002308E-4 1102 84-85 0.093445288508196 1102 86-87 -0.0059315202911278675 1102 88-89 -0.0825939529991544 1102 90-91 -0.05276225122374356 1102 92-93 0.04359641629097766 1102 94-95 0.0143605271493783 1102 96-97 -0.00353425983983513 1102 98-99 -0.15428723758720864 1102 100-101 -0.004427482303118069 1103 1 0.003911825526056845 1103 2 -0.1245204597229197 1103 3 -0.20231828043006672 1103 4 -0.10143569532174723 1103 5 -0.11108975865811033 1103 6 -0.11511321300663724 1103 7 0.012706535802628594 1103 8 -0.09198306472310946 1103 9 -0.0041779587113595085 1103 10-11 0.024865130694095683 1103 12-13 -0.012672692262192697 1103 14-15 -0.011771421845189423 1103 16-17 -0.10421765248692338 1103 18-19 -0.1339124637753386 1103 20-21 -0.20139507394234357 1103 22-23 -0.10841714055472806 1103 24-25 -0.13690533077153333 1103 26-27 -0.21290199181382974 1103 28-29 -0.2304913964575377 1103 30-31 -0.1978646144081253 1103 32-33 -0.23188300022287223 1103 34-35 -0.12456960517883431 1103 36-37 -0.34674456231555695 1103 38-39 -0.34778734973536984 1103 40-41 -0.2027243381684869 1103 42-43 -0.17395517967241148 1103 44-45 -0.22003508799075888 1103 46-47 -0.07140631170410927 1103 48-49 -0.06284284352293668 1103 50-51 -0.2702582726324394 1103 52-53 -0.35729887611311995 1103 54-55 -0.16202251776012133 1103 56-57 -0.2755612224174655 1103 58-59 -0.2231957969864098 1103 60-61 -0.13776958386561944 1103 62-63 -0.0175517396408722 1103 64-65 -0.06676839690968706 1103 66-67 -0.16484914922294536 1103 68-69 0.005433410807228256 1103 70-71 -0.15367105662102887 1103 72-73 -0.2901104085772772 1103 74-75 -0.09944888623590487 1103 76-77 -0.12211265777239078 1103 78-79 -0.11077207445181259 1103 80-81 -0.06561132124321034 1103 82-83 -0.05185698211153067 1103 84-85 -0.02464798690666825 1103 86-87 0.06678834742577067 1103 88-89 0.24340710126275056 1103 90-91 0.019889426926152254 1103 92-93 0.16043912297460494 1103 94-95 -0.3232014514651631 1103 96-97 -0.2762071163194122 1103 98-99 0.204885364636052 1103 100-101 0.7567788213918014 1104 1 0.08852461976593418 1104 2 -0.006770073781439123 1104 3 0.027754449302022977 1104 4 -0.02243475294350361 1104 5 0.22008820943865004 1104 6 0.2002844557677932 1104 7 0.09393723136449239 1104 8 0.13229965912000097 1104 9 0.2237097919974289 1104 10-11 0.2526811249196399 1104 12-13 0.16546330760018435 1104 14-15 0.3228298842877635 1104 16-17 0.23188299888284547 1104 18-19 0.2583220236866026 1104 20-21 0.3108278005163285 1104 22-23 0.2589716940422093 1104 24-25 0.30649105793322207 1104 26-27 0.22566126193853364 1104 28-29 0.14926123337633612 1104 30-31 0.08829317114673785 1104 32-33 0.11047719658548516 1104 34-35 0.1793889725958877 1104 36-37 0.19667353007427124 1104 38-39 0.23682190970237116 1104 40-41 0.389073530534354 1104 42-43 0.2660434393982243 1104 44-45 0.3197861075782953 1104 46-47 0.3280202019983207 1104 48-49 0.31652472853222946 1104 50-51 0.30567717151190976 1104 52-53 0.1742425167733046 1104 54-55 0.059486564678302045 1104 56-57 0.21565750430438868 1104 58-59 0.27988643264423985 1104 60-61 0.17208315863417312 1104 62-63 0.3723768785649142 1104 64-65 0.5090220025205952 1104 66-67 0.3224713977099185 1104 68-69 0.404550511496538 1104 70-71 0.4182693541559672 1104 72-73 0.3337858200009407 1104 74-75 0.29282978625266054 1104 76-77 0.3200969800305735 1104 78-79 0.4848954599695041 1104 80-81 0.4951210567666493 1104 82-83 0.5920093467537342 1104 84-85 0.7207527053018445 1104 86-87 0.7435286895804722 1104 88-89 0.5462817908776678 1104 90-91 0.7170675236897104 1104 92-93 0.779137896327569 1104 94-95 1.0569828955355192 1104 96-97 1.258839386359142 1104 98-99 1.52451277763927 1104 100-101 1.959115372730441 1105 1 0.09190130923908058 1105 2 0.1390448441105967 1105 3 0.10539854476767019 1105 4 0.11484938840023062 1105 5 0.11627843026291629 1105 6 0.031248210275634847 1105 7 0.029290706585470616 1105 8 0.14990187290104018 1105 9 0.14420436965384198 1105 10-11 0.031775488995563705 1105 12-13 0.15931012056891092 1105 14-15 0.19846731371946902 1105 16-17 0.1281717878102313 1105 18-19 0.2360656387791309 1105 20-21 0.23664859345768008 1105 22-23 0.13315768375746728 1105 24-25 0.10575833583597927 1105 26-27 0.21681599458597844 1105 28-29 0.1345438444746634 1105 30-31 0.1433987183107277 1105 32-33 0.14211365399906128 1105 34-35 0.14495418137362037 1105 36-37 0.22285434057246079 1105 38-39 0.22852206065308422 1105 40-41 0.20579309622963393 1105 42-43 0.23997717772284233 1105 44-45 0.20241418694708813 1105 46-47 0.020776593127287413 1105 48-49 0.23053296384845368 1105 50-51 0.11350608949032193 1105 52-53 -0.03849636143872459 1105 54-55 0.25252231342624754 1105 56-57 0.10443181726153483 1105 58-59 0.19854969301847092 1105 60-61 0.24138873414869266 1105 62-63 0.2587015057810831 1105 64-65 0.2772575285631902 1105 66-67 0.2885235914723694 1105 68-69 0.3505697142220896 1105 70-71 0.1069119908624856 1105 72-73 0.29265108155163944 1105 74-75 0.4143847517550405 1105 76-77 0.3126222728742114 1105 78-79 0.4064883156362775 1105 80-81 0.4539671658517719 1105 82-83 0.6637983993020029 1105 84-85 0.7251870500398852 1105 86-87 0.6459766430602336 1105 88-89 0.7401724471338511 1105 90-91 0.8625810037317478 1105 92-93 0.98485038146611 1105 94-95 1.0284980556269545 1105 96-97 1.1147630731770448 1105 98-99 1.403139943545245 1105 100-101 1.511997770004303 1106 1 -0.1263442051538597 1106 2 -0.028639418013501228 1106 3 -0.06795070328531594 1106 4 0.1460078550537105 1106 5 0.0782344895583833 1106 6 -0.08854938080028774 1106 7 -0.15763007705351129 1106 8 -0.2451713850975139 1106 9 -0.28274487464058495 1106 10-11 -0.5609846099172131 1106 12-13 -0.28253411633106396 1106 14-15 -0.13890982180848965 1106 16-17 -0.027330170556396638 1106 18-19 -0.32482017041023425 1106 20-21 -0.13592419863597627 1106 22-23 -0.13043303614782076 1106 24-25 -0.14596238014891583 1106 26-27 -0.37462273539642155 1106 28-29 -0.1841383676678845 1106 30-31 -0.34439666147876835 1106 32-33 -0.2956012560538923 1106 34-35 0.06840509326826094 1106 36-37 -0.31683671097358257 1106 38-39 -0.150459338126538 1106 40-41 -0.17585962113131615 1106 42-43 0.03877934536821215 1106 44-45 0.09276979897385473 1106 46-47 -0.07796653379669038 1106 48-49 0.11002437840880219 1106 50-51 6.994209687363195E-4 1106 52-53 0.0930969758863398 1106 54-55 -0.09349350802541778 1106 56-57 -0.13913673757717504 1106 58-59 0.01661735441602019 1106 60-61 -0.1592559372826443 1106 62-63 -0.14511379855530748 1106 64-65 -0.018044088087648902 1106 66-67 0.03901311787808481 1106 68-69 -0.12410450471509904 1106 70-71 0.11404320690819958 1106 72-73 0.08697416741334507 1106 74-75 0.15569086197378468 1106 76-77 0.12087787273786077 1106 78-79 0.09471663461486912 1106 80-81 -0.06160893534120504 1106 82-83 -0.0601487730859418 1106 84-85 -0.20537250043627964 1106 86-87 0.0559856114032371 1106 88-89 0.35071823710235606 1106 90-91 0.3998017152794553 1106 92-93 0.4499580198529145 1106 94-95 0.5466748718860686 1106 96-97 0.7832174486896086 1106 98-99 0.9502270069453722 1106 100-101 1.278173283295775 1107 1 0.022369442158989727 1107 2 -0.06336429962646761 1107 3 0.005216177596889082 1107 4 -0.11033811880528077 1107 5 -0.08666754753062378 1107 6 -0.10977945862590843 1107 7 0.12067156840090831 1107 8 -0.010247482002526453 1107 9 -0.00548252339635269 1107 10-11 0.04217412612324267 1107 12-13 -0.013565834184653625 1107 14-15 0.10537819447292662 1107 16-17 0.08753057383871976 1107 18-19 0.10447784862574849 1107 20-21 0.05596356999263463 1107 22-23 0.1670735879961569 1107 24-25 0.21910121008286154 1107 26-27 0.14690786435023284 1107 28-29 0.20569580004659826 1107 30-31 0.141909306529989 1107 32-33 0.18565372142117553 1107 34-35 0.2316546473950254 1107 36-37 0.09623945518325172 1107 38-39 0.14327745428387573 1107 40-41 0.1701867197106708 1107 42-43 0.1595209394109105 1107 44-45 0.0684178332814227 1107 46-47 0.1458830202267123 1107 48-49 0.18673860661011332 1107 50-51 0.2187605041810059 1107 52-53 0.18137742201280815 1107 54-55 0.0918196006735954 1107 56-57 0.050620762163134714 1107 58-59 0.05158745984933688 1107 60-61 0.34756368806496596 1107 62-63 0.30869859204360495 1107 64-65 0.3403192586774395 1107 66-67 0.286222243408389 1107 68-69 0.19523672952817606 1107 70-71 0.2921828082554043 1107 72-73 0.33313172462233354 1107 74-75 0.4562391400581163 1107 76-77 0.34994056476309865 1107 78-79 0.3490311784804767 1107 80-81 0.6719431097324318 1107 82-83 0.5801888915874116 1107 84-85 0.5888035759515873 1107 86-87 0.803481434329413 1107 88-89 0.8113346197073312 1107 90-91 0.8824838488765643 1107 92-93 1.0376286364577432 1107 94-95 1.0913115504444129 1107 96-97 1.2425734738827998 1107 98-99 1.3400828804563965 1107 100-101 1.4618508343857926 1108 1 0.23202515647050959 1108 2 0.21612246301630478 1108 3 0.14269487800572023 1108 4 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0.19628192058271665 2316 94-95 0.4130715851483586 2316 96-97 0.3740373988998087 2316 98-99 0.4888258029446497 2316 100-101 0.24488023440708062 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7668.0 3 1793.0 4 1375.0 5 1495.0 6 1703.0 7 2110.0 8 2558.0 9 3234.0 10 4049.0 11 5020.0 12 6564.0 13 8333.0 14 9580.0 15 11735.0 16 14585.0 17 18556.0 18 16546.0 19 12360.0 20 12701.0 21 13209.0 22 14931.0 23 16600.0 24 18669.0 25 21756.0 26 25053.0 27 29269.0 28 34987.0 29 41946.0 30 51731.0 31 63838.0 32 80469.0 33 104704.0 34 139940.0 35 194216.0 36 279848.0 37 382761.0 38 294573.0 39 32674.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39979144120396 19.54205017441399 14.725686548159844 22.332471836222204 2 14.840955650715909 20.665052287099485 40.45744011012279 24.03655195206182 3 19.43962030286233 25.20308976373019 29.697516638024997 25.659773295382482 4 12.189625036067344 16.56914798197214 38.45301795863133 32.78820902332919 5 13.218394580043851 38.24511384808498 34.20647437552712 14.330017196344047 6 30.949102646921155 36.91329344198822 17.81023203903812 14.327371872052504 7 28.240770460621455 33.157698666918726 20.78930461713277 17.812226255327044 8 26.141772159575936 34.66328442424803 19.87269587262309 19.322247543552944 9 26.09866631221606 17.317775916799796 20.824714236835334 35.7588435341488 10-11 24.59102442278921 27.050184990497755 27.464695119485665 20.894095467227366 12-13 25.92827718668655 24.44726932699841 27.96143445626627 21.663019030048776 14-15 22.567881006811792 25.803310908694417 26.80801020265058 24.82079788184322 16-17 21.608005404193708 29.428468101635435 27.070325027477132 21.893201466693725 18-19 21.46602679971425 28.112484304248298 29.376862547794815 21.044626348242645 20-21 22.603597452785518 26.78575128525578 29.181357891114214 21.42929337084449 22-23 21.896751908691098 26.897036175113172 29.269485831529824 21.93672608466591 24-25 22.147992019825118 27.11975922025525 29.18645876707511 21.545789992844526 26-27 21.82820103469728 27.101473005842596 29.575493128718584 21.494832830741537 28-29 21.286861482101482 27.66996817771955 29.52565292283805 21.51751741734092 30-31 21.660277588170672 27.1239947910204 29.25699258506353 21.9587350357454 32-33 21.501617386909274 27.26181450593884 29.299646196613594 21.936921910538288 34-35 22.264860978251118 27.253266881573413 28.950238263425458 21.531633876750014 36-37 22.041933883850636 26.938119992086136 29.291206235276785 21.728739888786443 38-39 21.645769621159612 27.369802280536803 29.374943304956542 21.60948479334704 40-41 21.84863311970104 27.331112366037647 29.22974883361317 21.590505680648146 42-43 21.529073315316886 27.351594885148305 28.75374447725139 22.365587322283414 44-45 21.83148103968363 27.28514977396424 28.65096782932885 22.23240135702328 46-47 22.15731746706504 27.686922914784855 28.31756866388424 21.838190954265862 48-49 22.111557258819282 27.637961707906335 28.26468873299134 21.985792300283048 50-51 21.680919615994306 27.78493598557148 28.525924226003692 22.008220172430526 52-53 22.139197402210677 28.139658982011785 27.760713593272364 21.960430022505175 54-55 21.777848095827103 27.572254744320034 28.150601042403302 22.499296117449564 56-57 22.074118844138383 27.88276236200533 27.91108710147963 22.132031692376653 58-59 22.472805141133843 27.423902775837355 28.09196014443965 22.011331938589148 60-61 22.183954245501212 27.702450242437305 28.57129864693383 21.542296865127657 62-63 21.845065903331673 27.79996311333554 28.50727753677929 21.8476934465535 64-65 21.66483803400979 27.946525947425823 28.242761911522813 22.14587410704158 66-67 21.82792925412869 27.829666852519125 27.76125428431182 22.581149609040363 68-69 21.656386307395163 27.738249378820395 28.219099867490105 22.386264446294334 70-71 22.04887055157546 27.700723389666592 28.046868111559608 22.203537947198342 72-73 22.011662728308355 27.362667597084346 28.385562794476815 22.240106880130487 74-75 21.58115717129169 28.278619147935224 27.8956948045484 22.244528876224685 76-77 21.852445474950937 27.73058600482679 27.936246507574364 22.480722012647906 78-79 21.92641688541287 27.68514595166031 27.96843586421994 22.420001298706875 80-81 22.042279676497564 27.98760968486052 27.740637151238147 22.22947348740377 82-83 22.008120098633682 27.694564031045353 28.11256973077856 22.184746139542405 84-85 21.91343045035135 27.767384032967808 27.883249373490887 22.435936143189956 86-87 21.855295219864793 27.854593819388366 27.901640205811518 22.388470754935323 88-89 21.84181876109647 27.710279913378372 27.637413044230748 22.810488281294415 90-91 21.842543067027293 27.570149425705587 27.962614262554226 22.624693244712894 92-93 22.188184139151616 27.503995088409884 28.036943040461242 22.270877731977258 94-95 21.903641823172805 27.314343318481725 28.075552450767994 22.70646240757748 96-97 21.552973126215672 27.347091204304736 28.447405883134742 22.65252978634485 98-99 21.700237450343767 27.502672800905835 27.806327241798336 22.990762506952063 100-101 21.701438669896667 26.904796972646654 28.480292973590494 22.91347138386619 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2690.0 1 1567.5 2 409.5 3 361.5 4 306.0 5 277.0 6 427.5 7 766.0 8 1252.0 9 1594.5 10 1588.0 11 1529.5 12 1687.5 13 1854.5 14 2026.5 15 2203.0 16 2303.0 17 2482.5 18 2652.0 19 2889.0 20 3252.0 21 3701.0 22 4404.5 23 5392.0 24 6545.5 25 8021.5 26 10375.0 27 13147.5 28 15477.5 29 18892.5 30 23290.0 31 27419.0 32 32473.0 33 37824.0 34 43528.5 35 48437.0 36 53838.0 37 60310.5 38 63639.0 39 64624.0 40 65067.0 41 67404.5 42 69990.0 43 71967.5 44 73894.5 45 74504.0 46 73929.5 47 78681.0 48 92744.0 49 92042.5 50 77283.5 51 68645.0 52 63549.0 53 60237.5 54 56565.0 55 51380.5 56 45657.5 57 39983.0 58 35503.0 59 31040.0 60 26243.0 61 21701.0 62 17427.0 63 13404.5 64 10138.0 65 7765.5 66 5732.0 67 4530.0 68 3398.0 69 2254.0 70 1599.0 71 1198.5 72 936.0 73 760.0 74 583.5 75 460.5 76 310.0 77 180.0 78 104.5 79 75.0 80 46.5 81 25.5 82 22.5 83 15.5 84 11.0 85 8.0 86 4.5 87 3.0 88 5.0 89 7.0 90 5.5 91 2.0 92 0.5 93 2.0 94 2.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24224173445888675 2 0.21486095556397092 3 0.20633912935174117 4 0.21208758016945825 5 0.21349948037030106 6 0.24047685920783324 7 0.24173748438715714 8 0.23064398280910658 9 0.22660998223526996 10-11 0.23513180844749976 12-13 0.23664455866268846 14-15 0.22396266935868972 16-17 0.23596382106585356 18-19 0.2270385947962401 20-21 0.21955048123105594 22-23 0.22013036881354497 24-25 0.21375160540616586 26-27 0.22187003156101198 28-29 0.2347284083901161 30-31 0.2337955457574164 32-33 0.2341233083040406 34-35 0.2403003716827279 36-37 0.21871846861270217 38-39 0.21957569373464245 40-41 0.21559211816797882 42-43 0.22164311902873368 44-45 0.23674540867703436 46-47 0.24072898424369799 48-49 0.24453607228525626 50-51 0.2440066097099402 52-53 0.2598652744658353 54-55 0.2435527846453836 56-57 0.21720571839751343 58-59 0.2040447915253716 60-61 0.2002629159873998 62-63 0.20714592946650848 64-65 0.20056546603043754 66-67 0.2004141910089187 68-69 0.20399436651819866 70-71 0.2045994666042741 72-73 0.20691901693423018 74-75 0.20986887985384814 76-77 0.22169354403590663 78-79 0.21438191799582781 80-81 0.2106756799686155 82-83 0.20689380443064367 84-85 0.20782666706334338 86-87 0.21405415544920361 88-89 0.2113564175654504 90-91 0.2202060063243044 92-93 0.20928899227135914 94-95 0.20777624205617043 96-97 0.21682753084371625 98-99 0.2225507691578469 100-101 0.2011201411093401 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1983143.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.930881680342054 #Duplication Level Percentage of deduplicated Percentage of total 1 77.04531603659855 41.551218231943565 2 12.916514386827927 13.931980182369056 3 4.289903615856908 6.940748529805514 4 1.8838624432319029 4.063934501119195 5 0.9877072788062374 2.6633962194055907 6 0.583357082425103 1.8876577073794663 7 0.41116499649278576 1.5522143543865479 8 0.2918293444561245 1.2590891077372028 9 0.20943325137638036 1.0165427909918014 >10 1.2136598957737066 12.273198445303215 >50 0.10697468682271787 4.01006799149442 >100 0.05643457710789514 5.3207208322562005 >500 0.00215549505227366 0.8103478929271382 >1k 0.0014994748189729804 1.9898582014932589 >5k 1.8743435237162254E-4 0.7290250113878274 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7622 0.38433940467228034 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6802 0.34299089879045536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4857 0.24491425983905346 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4469 0.22534935705594605 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3834 0.19332947750111817 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3131 0.1578806974585292 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3020 0.15228352166233095 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2894 0.14592997075853834 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2506 0.1263650679754309 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2252 0.11355711615349978 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2153 0.108565040443377 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.5212503586478634E-5 0.0 18-19 0.0 0.0 0.0 5.042500717295727E-5 0.0 20-21 0.0 0.0 0.0 1.0085001434591454E-4 0.0 22-23 0.0 0.0 0.0 2.0170002869182907E-4 0.0 24-25 0.0 0.0 0.0 3.025500430377436E-4 0.0 26-27 0.0 0.0 0.0 5.798875824890087E-4 0.0 28-29 0.0 0.0 0.0 0.001311050186496889 0.0 30-31 0.0 0.0 0.0 0.0025464628622343423 0.0 32-33 0.0 0.0 0.0 0.005193775738814599 0.0 34-35 0.0 0.0 0.0 0.008194063665605556 0.0 36-37 0.0 0.0 0.0 0.011749026671299043 0.0 38-39 0.0 0.0 0.0 0.01573260223796267 0.0 40-41 0.0 0.0 0.0 0.020825527962431353 0.0 42-43 0.0 0.0 0.0 0.02735556639132932 0.0 44-45 0.0 0.0 0.0 0.03527229251748361 0.0 46-47 0.0 0.0 0.0 0.04424794379427001 0.0 48-49 0.0 0.0 0.0 0.053727845142785974 0.0 50-51 0.0 0.0 0.0 0.06825024720859767 0.0 52-53 0.0 0.0 0.0 0.09908513909486104 0.0 54-55 0.0 0.0 0.0 0.1425767077815367 0.0 56-57 0.0 0.0 0.0 0.18584136393593403 0.0 58-59 0.0 0.0 0.0 0.2521502483683728 0.0 60-61 5.042500717295727E-5 0.0 0.0 0.33494811014636866 0.0 62-63 5.042500717295727E-5 0.0 0.0 0.3980045816161517 0.0 64-65 5.042500717295727E-5 0.0 0.0 0.45190891428404306 0.0 66-67 5.042500717295727E-5 0.0 0.0 0.5768368695550448 0.0 68-69 5.042500717295727E-5 0.0 0.0 0.7051180878030481 0.0 70-71 5.042500717295727E-5 0.0 0.0 0.7724858973861188 0.0 72-73 5.042500717295727E-5 0.0 0.0 0.8975147026714665 0.0 74-75 5.042500717295727E-5 0.0 0.0 1.0833560666074005 0.0 76-77 5.042500717295727E-5 0.0 0.0 1.271340493348185 0.0 78-79 5.042500717295727E-5 0.0 0.0 1.46018214521091 0.0 80-81 5.042500717295727E-5 0.0 0.0 1.6977091414991254 0.0 82-83 5.042500717295727E-5 0.0 0.0 1.9619866040925944 0.0 84-85 7.56375107594359E-5 0.0 0.0 2.2358448180489257 0.0 86-87 1.0085001434591454E-4 0.0 0.0 2.5867776554691213 0.0 88-89 1.0085001434591454E-4 0.0 0.0 2.9952958510808347 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9510 0.0 26.680998 1 TACATGG 9900 0.0 24.809603 2 ACATGGG 9840 0.0 23.753273 3 GAGTACT 6100 0.0 21.414448 12-13 TCGCGCT 190 0.0 21.242456 60-61 CATGGGG 3560 0.0 20.6856 4 GTACTTT 6420 0.0 20.420279 14-15 AGTACTT 6530 0.0 19.74971 12-13 CATGGGA 6240 0.0 19.491344 4 CGCGCTT 235 0.0 19.195312 62-63 GTATCAA 17340 0.0 18.688591 1 AGAGTAC 12040 0.0 18.641201 10-11 TCGCGGA 195 1.4551915E-11 18.2669 82-83 CTCGCGC 235 0.0 18.185032 60-61 CGCGGAA 185 1.4188117E-10 17.97068 82-83 AGTACAT 7215 0.0 17.317446 2 GTGTAGC 965 0.0 17.233839 1 TACTTTT 7385 0.0 17.108767 14-15 GAGTACA 8250 0.0 16.990627 1 ATTCGCG 210 4.7293724E-11 16.961908 80-81 >>END_MODULE