FastQCFastQC Report
Thu 26 May 2016
SRR937236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983143
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT91450.46113669059669427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87190.43965563754101444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66290.33426737254953376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45730.23059355780193358No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35360.17830282536357692No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34280.17285692458889754No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27510.13871919473280545No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT27010.1361979443741576No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24090.12147384227965405No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23150.11673389160539607No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21050.10614464009907505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG91550.023.2636341
GAGTACT76850.022.0664112-13
TACATGG94900.021.9221132
ACATGGG93900.021.5485733
AGTACTT80450.021.46276512-13
GTACTTT79900.020.98468414-15
AGAGTAC133750.019.7996910-11
CATGGGG35800.019.7686824
TATAGCG2502.1750202E-718.9991635
TACTTTT89850.018.66083514-15
GTATCAA202100.018.2067551
CATGGGA58700.018.1252654
GTACACG3156.7429937E-918.1104241
TACCTGG16950.017.6540892
TATACGA1350.00940512317.5918182
ACTTTTT96000.017.51485316-17
GTACCTG18350.017.0988041
TAGCACC6500.016.8069534
AGTACAT71600.016.7834782
GTATAGG7100.016.7393861
Produced by FastQC (version 0.11.5)