##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937236_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1983143 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20039603800634 33.0 31.0 34.0 31.0 34.0 2 32.39931563180265 34.0 31.0 34.0 31.0 34.0 3 32.46484797112463 34.0 31.0 34.0 31.0 34.0 4 36.10034173027361 37.0 35.0 37.0 35.0 37.0 5 36.029289869666485 37.0 35.0 37.0 35.0 37.0 6 36.077584420286385 37.0 35.0 37.0 35.0 37.0 7 36.023809175636856 37.0 35.0 37.0 35.0 37.0 8 36.00190707377128 37.0 35.0 37.0 35.0 37.0 9 37.71385018629519 39.0 37.0 39.0 35.0 39.0 10-11 37.634596194021306 39.0 37.0 39.0 35.0 39.0 12-13 37.54561118386319 39.0 37.0 39.0 35.0 39.0 14-15 38.74060393022591 40.0 38.0 41.0 35.0 41.0 16-17 38.721264931474934 40.0 38.0 41.0 35.0 41.0 18-19 38.719523503852216 40.0 38.0 41.0 34.5 41.0 20-21 38.631230829042586 40.0 38.0 41.0 34.0 41.0 22-23 38.48797615703961 40.0 38.0 41.0 34.0 41.0 24-25 38.42964602149215 40.0 38.0 41.0 34.0 41.0 26-27 38.38843189825444 40.0 38.0 41.0 34.0 41.0 28-29 38.28620502908767 40.0 38.0 41.0 34.0 41.0 30-31 38.369289557031436 40.0 38.0 41.0 34.0 41.0 32-33 38.319796151866 40.0 38.0 41.0 34.0 41.0 34-35 38.19932879272952 40.0 38.0 41.0 34.0 41.0 36-37 38.03980146666176 40.0 37.5 41.0 33.5 41.0 38-39 37.93046996610936 40.0 37.0 41.0 33.0 41.0 40-41 37.778572447876925 40.0 37.0 41.0 33.0 41.0 42-43 37.58179137863482 40.0 37.0 41.0 33.0 41.0 44-45 37.309257073241824 40.0 37.0 41.0 32.5 41.0 46-47 36.98985852255738 40.0 36.5 41.0 31.5 41.0 48-49 36.68097182099324 40.0 36.0 41.0 31.0 41.0 50-51 36.4502080787921 39.0 36.0 41.0 30.0 41.0 52-53 36.22863681539859 39.0 35.5 41.0 30.0 41.0 54-55 36.01525633804522 39.0 35.0 40.0 29.5 41.0 56-57 35.75951129091548 39.0 35.0 40.0 29.0 41.0 58-59 35.504307304112714 38.0 35.0 40.0 28.0 41.0 60-61 35.17430815629534 38.0 34.0 40.0 28.0 41.0 62-63 34.8337356408489 38.0 34.0 40.0 27.0 41.0 64-65 34.4499957895119 37.0 34.0 40.0 26.0 41.0 66-67 34.03840141633761 36.5 33.5 39.0 26.0 41.0 68-69 33.610817525513795 36.0 33.0 39.0 26.0 40.0 70-71 33.20272063083701 35.5 32.5 38.5 25.0 40.0 72-73 32.73366948323948 35.0 32.0 38.0 24.5 40.0 74-75 32.269505275212126 35.0 32.0 37.0 23.5 39.0 76-77 30.98121819757829 34.0 30.5 36.0 21.5 38.5 78-79 31.594636644962065 34.5 31.0 36.0 23.0 38.5 80-81 31.39937336843586 34.5 31.0 36.0 22.0 37.5 82-83 31.05603151159548 34.0 31.0 35.5 20.5 37.0 84-85 30.692931876319562 34.0 31.0 35.0 19.5 36.5 86-87 30.309467849771803 34.0 30.5 35.0 18.0 36.0 88-89 29.841888860258692 34.0 30.0 35.0 14.5 36.0 90-91 29.338526016530327 34.0 29.0 35.0 7.0 35.0 92-93 29.010758427405385 34.0 29.0 35.0 2.0 35.0 94-95 28.5723142506617 34.0 29.0 35.0 2.0 35.0 96-97 28.094366366923616 33.5 29.0 35.0 2.0 35.0 98-99 27.353317940259476 33.0 27.0 35.0 2.0 35.0 100-101 24.798893473642597 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06933514709670163 1101 2 -0.03960592787772299 1101 3 -0.0705597254148671 1101 4 -0.036509844469946984 1101 5 -0.002513267793240459 1101 6 -0.07852619185349852 1101 7 -0.05355281718324534 1101 8 -0.0444308427930693 1101 9 -0.0411760123982674 1101 10-11 -0.02185126169698748 1101 12-13 0.010643385598392285 1101 14-15 -0.16885874584599492 1101 16-17 -0.08915459234616918 1101 18-19 -0.08845614079561415 1101 20-21 -0.06300870043461515 1101 22-23 -0.06032903847819426 1101 24-25 -0.061836926030238715 1101 26-27 -0.09039716513157003 1101 28-29 -0.2735468250592774 1101 30-31 -0.2217396168897281 1101 32-33 -0.13531044590018126 1101 34-35 -0.04497527014922298 1101 36-37 -0.0035949897007654386 1101 38-39 -0.12256996771827744 1101 40-41 -0.12618581471208756 1101 42-43 -0.06998379675061983 1101 44-45 -0.12223748339181384 1101 46-47 -0.04386144268620029 1101 48-49 -0.035874592451840215 1101 50-51 -0.0052683012425305265 1101 52-53 -0.00858477823867787 1101 54-55 -0.2755874239378713 1101 56-57 -0.08836127840422137 1101 58-59 0.06290396313076485 1101 60-61 -0.06501175329645292 1101 62-63 -0.16177950678790864 1101 64-65 -0.25279005728067006 1101 66-67 -0.02891641867663708 1101 68-69 -0.05315068007590895 1101 70-71 -0.12022521232501049 1101 72-73 -0.10656193751301402 1101 74-75 -0.25591750278660186 1101 76-77 -0.16644859820905822 1101 78-79 -0.17296785522092506 1101 80-81 -0.285510245885515 1101 82-83 -0.24463883214774285 1101 84-85 -0.1861046424213093 1101 86-87 -0.20358004206122615 1101 88-89 -0.31404655784264435 1101 90-91 -0.30099126356485684 1101 92-93 -0.316415634272591 1101 94-95 -0.15543926057568314 1101 96-97 0.017595218004668567 1101 98-99 0.11415469425344682 1101 100-101 0.6908476965840897 1102 1 -0.0104799523257455 1102 2 -0.008470086858324066 1102 3 0.0075930513121207355 1102 4 0.04417973480828152 1102 5 0.024485739495055725 1102 6 -0.07764008775403397 1102 7 -0.048594760972378026 1102 8 -0.04802890689060746 1102 9 -0.14083188762380416 1102 10-11 -0.0445444679265421 1102 12-13 -0.1606554389667565 1102 14-15 -0.10682217370474234 1102 16-17 -0.1463524400188092 1102 18-19 -0.10911016127802498 1102 20-21 -0.0033289588606280063 1102 22-23 0.025749039558576214 1102 24-25 -6.83133665177138E-4 1102 26-27 -0.03169752576333451 1102 28-29 -0.08914638395597052 1102 30-31 -0.17450717332871335 1102 32-33 -0.11382660555023705 1102 34-35 -0.023391585469312304 1102 36-37 -0.10911233464400993 1102 38-39 -0.09687592926159283 1102 40-41 -0.09209029429501214 1102 42-43 -0.07396335560145673 1102 44-45 0.0859723341372316 1102 46-47 0.08983756620693129 1102 48-49 0.1495350673437983 1102 50-51 0.07854556875120977 1102 52-53 0.13984591191375983 1102 54-55 0.16699761569034877 1102 56-57 -0.0035496529018459455 1102 58-59 0.0900509601205357 1102 60-61 0.19359136606509253 1102 62-63 0.11407788203640479 1102 64-65 0.06807637599558092 1102 66-67 0.1322576635235535 1102 68-69 0.08870083042781118 1102 70-71 0.0828286944426253 1102 72-73 0.041271520769015524 1102 74-75 0.1424165527273047 1102 76-77 0.16922907082131644 1102 78-79 0.15271643952962677 1102 80-81 0.05004385002465028 1102 82-83 0.19994091607488684 1102 84-85 0.15103744832583743 1102 86-87 0.13669665626674998 1102 88-89 0.33379687340920583 1102 90-91 0.044447650275564854 1102 92-93 0.2271268104576727 1102 94-95 0.2066023553663996 1102 96-97 0.4567575802458599 1102 98-99 0.3910654350273042 1102 100-101 1.0355478082354956 1103 1 0.01845127512137168 1103 2 0.036637660803037875 1103 3 -0.03810396591796206 1103 4 -0.023209942966921915 1103 5 -0.05612503422380399 1103 6 -0.04910519761953225 1103 7 -0.06570452406599259 1103 8 -0.04399292234846541 1103 9 0.018275317779227862 1103 10-11 -0.07125266894379934 1103 12-13 -0.04709572247709559 1103 14-15 -0.012367172418912276 1103 16-17 -0.1004356103449382 1103 18-19 0.0030705201289151773 1103 20-21 -0.17523916274277695 1103 22-23 -0.09250249150665724 1103 24-25 -0.0478712449421721 1103 26-27 -0.11838531645627626 1103 28-29 -0.1380364416611073 1103 30-31 -0.20204982380831638 1103 32-33 -0.18677757306245013 1103 34-35 0.051291001405473935 1103 36-37 0.009731491465608144 1103 38-39 -0.021669818731659518 1103 40-41 -0.004081534379309915 1103 42-43 -0.09908958193729234 1103 44-45 -0.09630966579528177 1103 46-47 -0.010672459172148763 1103 48-49 -0.10148725252113167 1103 50-51 0.023299684852673863 1103 52-53 -0.08598909027970336 1103 54-55 -0.03773667190903751 1103 56-57 -0.2246209272903812 1103 58-59 -0.1498276019407001 1103 60-61 -0.05934770900044839 1103 62-63 -0.13586562302370453 1103 64-65 -0.22980579103627718 1103 66-67 -0.0826407095850854 1103 68-69 -0.051952253404628834 1103 70-71 -0.019480208950447775 1103 72-73 0.07347992018766547 1103 74-75 0.13592492922214916 1103 76-77 0.12689475343262657 1103 78-79 -0.05835459049356473 1103 80-81 -0.07409595487871456 1103 82-83 0.06364486629883004 1103 84-85 -0.10446315735111966 1103 86-87 0.01844035425911983 1103 88-89 0.13833345781008433 1103 90-91 0.1399744844992803 1103 92-93 0.22460135282538474 1103 94-95 0.32148408835886144 1103 96-97 0.3583895916402682 1103 98-99 0.6420409763838144 1103 100-101 1.3747336527330312 1104 1 0.14463216414295488 1104 2 0.16762965117706585 1104 3 0.154316994139009 1104 4 0.05095044137447502 1104 5 0.036847331400103656 1104 6 0.07620730834544531 1104 7 0.09174098283523335 1104 8 0.029294601520284402 1104 9 0.09549825468607764 1104 10-11 0.11567520860311475 1104 12-13 0.05480531767378238 1104 14-15 0.11704429009603956 1104 16-17 0.05261896535037636 1104 18-19 0.0296058084120574 1104 20-21 0.004401131079397658 1104 22-23 -0.014574361574879902 1104 24-25 0.06014606333428674 1104 26-27 -0.013778385274868299 1104 28-29 0.02886065482886835 1104 30-31 0.1110530514233048 1104 32-33 0.09764815944870975 1104 34-35 0.14414129852915636 1104 36-37 0.1834803305776802 1104 38-39 0.06069295612572034 1104 40-41 0.044945110723496384 1104 42-43 0.02466926066480113 1104 44-45 0.0854959299850222 1104 46-47 0.07402187117018855 1104 48-49 0.19881209808858813 1104 50-51 0.3030496989329734 1104 52-53 0.3679438366142236 1104 54-55 0.3316065547856013 1104 56-57 0.2593040887163838 1104 58-59 0.11008958000193303 1104 60-61 0.18376855841816564 1104 62-63 0.34043352869448285 1104 64-65 0.287463602596695 1104 66-67 0.31054240094182006 1104 68-69 0.36145593446962465 1104 70-71 0.31626684437958374 1104 72-73 0.36892810835414025 1104 74-75 0.29579354298351035 1104 76-77 0.29597541502504754 1104 78-79 0.26405070567690103 1104 80-81 0.21183831335479653 1104 82-83 0.1669635977573236 1104 84-85 0.20692934495652082 1104 86-87 0.2590204011494457 1104 88-89 0.37216321089944415 1104 90-91 0.2752801117393133 1104 92-93 0.3171167863606179 1104 94-95 0.6502750045772174 1104 96-97 0.7930688048121652 1104 98-99 0.9420290287526107 1104 100-101 1.4126230789380294 1105 1 0.042827904293041286 1105 2 0.041192880785359876 1105 3 0.05056259852616307 1105 4 0.00812528435475457 1105 5 7.152901547229362E-4 1105 6 -0.008301177172725716 1105 7 0.06557505514469852 1105 8 -0.042204602249505285 1105 9 0.06947847128704154 1105 10-11 0.05688787440917764 1105 12-13 0.05772429981660565 1105 14-15 0.14311933506773755 1105 16-17 0.06390635504985909 1105 18-19 0.12721921601629305 1105 20-21 -0.03219422904673053 1105 22-23 -0.04895913680422126 1105 24-25 -0.058840641926835247 1105 26-27 0.1164505733018899 1105 28-29 -0.10903050988257945 1105 30-31 0.04676700776398235 1105 32-33 0.07208779124317033 1105 34-35 0.06897262874230137 1105 36-37 0.0735588410477348 1105 38-39 0.011657886619346414 1105 40-41 0.03690424692533867 1105 42-43 0.06510938676365896 1105 44-45 0.12347539122249884 1105 46-47 0.11279829455825308 1105 48-49 0.24961614787133612 1105 50-51 0.18600666395276022 1105 52-53 0.2506785354410894 1105 54-55 0.38191727609184767 1105 56-57 0.2420384459706213 1105 58-59 0.27429025490066294 1105 60-61 0.18355629542383411 1105 62-63 0.3993184550697251 1105 64-65 0.3174451405957939 1105 66-67 0.28539143193685135 1105 68-69 0.3922704217361712 1105 70-71 0.36895308136902116 1105 72-73 0.34467401149535704 1105 74-75 0.5279331368878104 1105 76-77 0.6473122693216276 1105 78-79 0.3430431591174994 1105 80-81 0.33930748703447833 1105 82-83 0.22073018019380442 1105 84-85 0.24925897767566596 1105 86-87 0.2581002288699672 1105 88-89 0.5774056676947765 1105 90-91 0.7788966339980128 1105 92-93 0.9064957316770368 1105 94-95 0.9053421736158995 1105 96-97 0.7080561336873252 1105 98-99 0.8976001882136018 1105 100-101 1.4722005438298496 1106 1 -0.18188155114896887 1106 2 -0.03686067556981243 1106 3 0.018373137843056497 1106 4 -0.05311993718368058 1106 5 -0.10231886002169688 1106 6 -0.053895909341356685 1106 7 -0.15924428599008422 1106 8 -0.05335552046061309 1106 9 -0.1203144790106947 1106 10-11 -0.2053786054431086 1106 12-13 -0.20087862758001052 1106 14-15 -0.1594147699537487 1106 16-17 -0.07890223973398491 1106 18-19 -0.07157261558766947 1106 20-21 -0.041781298972132674 1106 22-23 -0.041968083744315265 1106 24-25 -0.0648933599142083 1106 26-27 -0.08739064944136743 1106 28-29 -0.14450779747563303 1106 30-31 -0.08869099110531664 1106 32-33 -0.23103789554240706 1106 34-35 -0.20191335271441346 1106 36-37 -0.18825778609314625 1106 38-39 -0.1365383697499709 1106 40-41 -0.1173622151781899 1106 42-43 -0.28826786256514936 1106 44-45 -0.0250907179417581 1106 46-47 -0.2935120153298456 1106 48-49 -0.2543185951657989 1106 50-51 -0.07570138255107395 1106 52-53 0.058417855256479356 1106 54-55 -0.0863987113107072 1106 56-57 -0.09668172241289597 1106 58-59 -0.06929307316895006 1106 60-61 -0.035717472381691096 1106 62-63 0.08452778861951771 1106 64-65 -0.06033704247011684 1106 66-67 -0.11171157924434993 1106 68-69 0.0020963429619555995 1106 70-71 0.0656830554192922 1106 72-73 -0.029071764691757096 1106 74-75 0.10012190328126991 1106 76-77 0.25492925321254134 1106 78-79 0.18421644076367016 1106 80-81 0.10008346273329494 1106 82-83 0.008586975906123229 1106 84-85 0.022340323887632962 1106 86-87 0.1404978720437171 1106 88-89 0.3355105858234104 1106 90-91 0.3027677999400282 1106 92-93 0.5125052784207398 1106 94-95 0.5970814840348915 1106 96-97 0.6070796648862462 1106 98-99 0.6258107166724294 1106 100-101 1.2334172368499416 1107 1 0.015413544839752547 1107 2 -0.11520645614385217 1107 3 -0.031661307188819876 1107 4 -0.059022363426606717 1107 5 -0.09310629980749496 1107 6 -0.0475942514836305 1107 7 -0.03826651220892785 1107 8 -0.034875101931511665 1107 9 -0.09529880690879367 1107 10-11 -0.06427529463729087 1107 12-13 -0.07909812693064566 1107 14-15 -0.07243757602743983 1107 16-17 -0.013492855491257671 1107 18-19 0.0689799106311284 1107 20-21 0.044462419912996154 1107 22-23 0.08550114441306533 1107 24-25 -0.003904076892837338 1107 26-27 0.0515689555544796 1107 28-29 0.05385359576776949 1107 30-31 0.007526153683194536 1107 32-33 0.015125863322730027 1107 34-35 -0.05311133180139649 1107 36-37 -0.014848368774913467 1107 38-39 -0.08309546865657325 1107 40-41 -0.03428327894826566 1107 42-43 -0.0801782672387219 1107 44-45 0.009920861067080011 1107 46-47 0.05297270508140883 1107 48-49 0.1092416102537257 1107 50-51 0.10583788917811177 1107 52-53 -0.03560765008805333 1107 54-55 0.14293530317119263 1107 56-57 -0.011173287385318531 1107 58-59 -0.10050316615761545 1107 60-61 0.09803532546963822 1107 62-63 -0.05629838567698897 1107 64-65 0.08271798120728846 1107 66-67 0.013622470380269647 1107 68-69 0.19578285027707665 1107 70-71 0.1463248699825428 1107 72-73 0.10265658002936817 1107 74-75 0.08069121014799663 1107 76-77 0.116916563590447 1107 78-79 -8.230198576271164E-4 1107 80-81 0.14948947327507867 1107 82-83 0.18768499153119222 1107 84-85 0.10558308720091247 1107 86-87 0.19976619548282315 1107 88-89 0.25955929053000304 1107 90-91 0.4319016559696145 1107 92-93 0.3901374292532829 1107 94-95 0.3657558335199198 1107 96-97 0.6650539094082468 1107 98-99 0.9171044441623586 1107 100-101 1.6981369194078333 1108 1 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-0.6107352950772764 2316 88-89 -0.617526463877212 2316 90-91 -0.44680036986358473 2316 92-93 -0.2745545220554817 2316 94-95 -0.3992325631821565 2316 96-97 -0.16911392806415293 2316 98-99 -0.31382654103584784 2316 100-101 -0.6332628880728954 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 10.0 5 38.0 6 73.0 7 164.0 8 405.0 9 733.0 10 1385.0 11 2196.0 12 3553.0 13 4894.0 14 6476.0 15 9140.0 16 12575.0 17 17911.0 18 15901.0 19 9606.0 20 8388.0 21 8566.0 22 8858.0 23 9701.0 24 11636.0 25 13818.0 26 17103.0 27 21352.0 28 27086.0 29 34312.0 30 43655.0 31 55321.0 32 71664.0 33 94601.0 34 131338.0 35 188521.0 36 293092.0 37 451771.0 38 373831.0 39 33458.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89021716367636 20.111622180114395 15.273365847750778 21.72479480845846 2 15.451634097994951 21.30239725526601 39.66496616734144 23.581002479397604 3 19.794840815816105 25.30876492517181 29.942671809345068 24.95372244966702 4 12.712799833395778 17.15362936510378 38.161796703515584 31.971774097984866 5 13.220675042080455 38.30213816104636 34.27855091961801 14.198635877255175 6 31.05061006694928 37.438449975619505 17.339748066579162 14.171191890852047 7 28.266947970973348 33.340762617723485 20.674000815876617 17.718288595426554 8 26.32523221976428 34.86445505946873 19.560868782533582 19.2494439382334 9 26.15222402015387 17.31125793752644 20.679950966723027 35.856567075596665 10-11 24.749135209818775 27.20594612584084 27.304930564660086 20.739988099680303 12-13 26.165636213131798 24.436208996702767 27.97594989275232 21.42220489741312 14-15 22.613144891719863 25.880307168973694 26.88923592499381 24.617312014312635 16-17 21.52232592405086 29.45405348983911 27.24450531303088 21.779115273079146 18-19 21.4090713579404 27.923074634557366 29.86844619878647 20.79940780871576 20-21 22.459146919813648 26.493248343664575 29.88022043796136 21.167384298560414 22-23 21.887010709354826 26.57998029373827 29.86282767711179 21.670181319795113 24-25 22.115916991658622 26.832964723371084 29.85700353885734 21.194114746112955 26-27 21.920035216958194 26.77429252817067 30.16311795134523 21.142554303525905 28-29 21.271817028426433 27.405445255975035 30.185206476377196 21.13753123922133 30-31 21.681392501573267 26.744232367321253 29.933978305068337 21.640396826037144 32-33 21.549000752845355 26.965579385853665 29.920484806188963 21.56493505511201 34-35 22.254194478159164 26.790201210906122 29.731315391779617 21.2242889191551 36-37 22.096411702445767 26.62276661114868 29.871955650152966 21.40886603625259 38-39 21.65575546950756 27.141903384093137 29.963653434896635 21.238687711502667 40-41 21.894565715338878 27.044528615093082 29.766675633925082 21.29423003564296 42-43 21.609649048708956 27.157125851116326 29.328050862608272 21.905174237566445 44-45 21.995673013770364 26.835533930989584 29.250371444613844 21.9184216106262 46-47 22.245294464393137 27.365701817771082 28.888587459401567 21.500416258434214 48-49 22.26947887002573 27.401838244099956 28.67809549742391 21.6505873884504 50-51 21.81726179100549 27.558400982682542 28.8581307550691 21.76620647124287 52-53 22.332157169744484 27.842275580297432 28.09082050331734 21.73474674664075 54-55 21.922624843493384 27.437733940517656 28.39517876421418 22.244462451774783 56-57 22.171699166424204 27.671907673828866 28.25204738135374 21.904345778393186 58-59 22.634726794789888 27.205602419996943 28.346543844795864 21.81312694041731 60-61 22.207929534077977 27.5679817340454 28.89156253482477 21.332526197051852 62-63 21.999951591993113 27.49844564915389 28.805184497537496 21.696418261315497 64-65 21.852898077898608 27.661206307969877 28.555242135964043 21.930653478167475 66-67 21.998337991763577 27.632324043198096 28.076013681312944 22.29332428372538 68-69 21.817236578501902 27.493856973501153 28.49053245277824 22.198373995218702 70-71 22.21786326049105 27.499454149297353 28.29848881296003 21.984193777251566 72-73 22.151680438576545 27.227234748074142 28.609711957231525 22.011372856117788 74-75 21.780401110762057 28.16993025717258 27.951816888645954 22.09785174341941 76-77 22.00627993039332 27.521540302439107 28.18460393425991 22.287575832907663 78-79 22.086430479294737 27.459694031141474 28.180418658664554 22.273456830899235 80-81 22.141149893603075 27.79639864053975 27.979441693476005 22.083009772381175 82-83 22.163291959448046 27.508591195253977 28.31143618563537 22.016680659662605 84-85 21.909106403406327 27.59942353812051 28.109929142386893 22.38154091608627 86-87 21.879284549828228 27.581041810903194 28.074929543658726 22.46474409560985 88-89 21.90311036571745 27.605901339439466 27.88336494140866 22.607623353434423 90-91 21.969393819509214 27.355821369559884 28.10154790892731 22.57323690200359 92-93 22.120912388579285 27.303404980281943 28.205814136138198 22.36986849500058 94-95 21.830069742827423 27.15636240049255 28.315028215312765 22.69853964136726 96-97 21.56730503044914 27.10213030527804 28.67985314220911 22.650711522063713 98-99 21.680017971504274 27.27992660123002 27.971884983381152 23.068170443884558 100-101 21.249493174753344 27.007103622312815 28.474577040463313 23.268826162470525 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 208.0 1 163.0 2 162.5 3 240.5 4 227.5 5 204.0 6 387.5 7 870.0 8 1531.5 9 1955.0 10 1922.0 11 1825.5 12 1914.0 13 2071.0 14 2313.5 15 2544.0 16 2721.5 17 2900.5 18 3052.0 19 3225.0 20 3564.0 21 4111.0 22 4766.0 23 5738.5 24 6865.5 25 8199.0 26 10472.5 27 13391.5 28 15684.5 29 18926.5 30 23322.0 31 27176.5 32 31843.0 33 37279.0 34 43272.5 35 47866.0 36 53314.0 37 60104.5 38 63691.0 39 64842.0 40 65565.0 41 67888.5 42 70024.5 43 71763.0 44 73686.5 45 74637.5 46 73788.5 47 78845.5 48 94427.0 49 93113.0 50 77201.5 51 68610.5 52 63379.5 53 60032.0 54 56425.5 55 51316.5 56 45473.0 57 39617.0 58 35094.0 59 30977.0 60 26160.5 61 21443.5 62 17119.0 63 13117.0 64 9975.5 65 7731.0 66 5644.5 67 4333.5 68 3225.0 69 2017.0 70 1377.5 71 1109.5 72 862.5 73 670.5 74 535.5 75 441.5 76 317.5 77 166.0 78 90.5 79 60.5 80 37.5 81 29.0 82 21.5 83 12.5 84 4.5 85 3.5 86 1.0 87 1.5 88 2.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0735196604581717 2 0.0 3 0.0 4 0.0 5 0.001865725265399419 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.512750215188718E-4 12-13 0.005874513335649522 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.572251219402736E-4 24-25 8.572251219402736E-4 26-27 3.7818755379717954E-4 28-29 0.002975075423204479 30-31 3.529750502107009E-4 32-33 0.0 34-35 0.0 36-37 1.0085001434591454E-4 38-39 6.051000860754872E-4 40-41 1.2606251793239315E-4 42-43 0.011723814167712565 44-45 3.7818755379717954E-4 46-47 0.0 48-49 2.5212503586478634E-5 50-51 0.0 52-53 2.5212503586478634E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.042500717295727E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.512750215188718E-4 82-83 4.0340005738365815E-4 84-85 5.5467507890253E-4 86-87 0.0 88-89 0.0 90-91 0.008950438773199915 92-93 0.0031515629483098296 94-95 0.0 96-97 0.0 98-99 1.7648752510535044E-4 100-101 0.010639676513493984 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1983143.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.52643775039763 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1719464180463 38.7334261768569 2 13.76540074708282 14.185641294076804 3 4.6899823665229805 7.249742533773268 4 2.0848204777626234 4.296934902727602 5 1.1204296963573592 2.8865875501527203 6 0.6720926674731437 2.0778324595832185 7 0.4416557746699398 1.5929864146444013 8 0.30664801303727646 1.264038380403868 9 0.24029571002268987 1.1143423749754557 >10 1.320934516070681 12.730976582680162 >50 0.11871575936277239 4.203183980199329 >100 0.06265940326607768 5.736039649191027 >500 0.0026510701952410023 0.9574156477392468 >1k 0.0014728167751338899 1.7316858376040447 >5k 2.9456335502677796E-4 1.2391662153919558 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9145 0.46113669059669427 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8719 0.43965563754101444 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6629 0.33426737254953376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4573 0.23059355780193358 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3536 0.17830282536357692 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3428 0.17285692458889754 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2751 0.13871919473280545 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2701 0.1361979443741576 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2409 0.12147384227965405 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2315 0.11673389160539607 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2105 0.10614464009907505 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.042500717295727E-5 0.0 2 0.0 0.0 0.0 5.042500717295727E-5 0.0 3 0.0 0.0 0.0 5.042500717295727E-5 0.0 4 0.0 0.0 0.0 5.042500717295727E-5 0.0 5 0.0 0.0 0.0 5.042500717295727E-5 0.0 6 0.0 0.0 0.0 5.042500717295727E-5 0.0 7 0.0 0.0 0.0 5.042500717295727E-5 0.0 8 0.0 0.0 0.0 5.042500717295727E-5 0.0 9 0.0 0.0 0.0 5.042500717295727E-5 0.0 10-11 0.0 0.0 0.0 5.042500717295727E-5 0.0 12-13 0.0 0.0 0.0 5.042500717295727E-5 0.0 14-15 0.0 0.0 0.0 5.042500717295727E-5 0.0 16-17 0.0 0.0 0.0 7.56375107594359E-5 0.0 18-19 0.0 0.0 0.0 1.0085001434591454E-4 0.0 20-21 0.0 0.0 0.0 1.512750215188718E-4 0.0 22-23 0.0 0.0 0.0 2.5212503586478636E-4 0.0 24-25 0.0 0.0 0.0 3.529750502107009E-4 0.0 26-27 0.0 0.0 0.0 6.051000860754872E-4 0.0 28-29 0.0 0.0 0.0 0.001311050186496889 0.0 30-31 0.0 0.0 0.0 0.002622100372993778 0.0 32-33 0.0 0.0 0.0 0.0053954757675064285 0.0 34-35 0.0 0.0 0.0 0.008168851162019078 0.0 36-37 0.0 0.0 0.0 0.011698601664126087 0.0 38-39 0.0 0.0 0.0 0.015556114712857318 0.0 40-41 0.0 0.0 0.0 0.020649040437326002 0.0 42-43 0.0 0.0 0.0 0.027204291369810448 0.0 44-45 0.0 0.0 0.0 0.03522186751031065 2.5212503586478634E-5 46-47 0.0 0.0 0.0 0.04429836880144296 5.042500717295727E-5 48-49 0.0 0.0 0.0 0.05385390766071836 5.042500717295727E-5 50-51 0.0 0.0 0.0 0.06860322225880837 5.042500717295727E-5 52-53 0.0 0.0 0.0 0.099715451684523 5.042500717295727E-5 54-55 0.0 0.0 0.0 0.14343393290347695 5.042500717295727E-5 56-57 0.0 0.0 0.0 0.18710198911525794 5.042500717295727E-5 58-59 0.0 0.0 0.0 0.2546210737198477 5.042500717295727E-5 60-61 0.0 0.0 0.0 0.3378475480588137 5.042500717295727E-5 62-63 0.0 0.0 0.0 0.4016099696290182 5.042500717295727E-5 64-65 0.0 0.0 0.0 0.4555395148004959 5.042500717295727E-5 66-67 0.0 0.0 0.0 0.5814255452077838 5.042500717295727E-5 68-69 0.0 0.0 0.0 0.7101858010239301 5.042500717295727E-5 70-71 0.0 0.0 0.0 0.7783604107217684 5.042500717295727E-5 72-73 0.0 0.0 0.0 0.9038934660788456 5.042500717295727E-5 74-75 0.0 0.0 0.0 1.0910458802012766 5.042500717295727E-5 76-77 0.0 0.0 0.0 1.2824339949262358 5.042500717295727E-5 78-79 0.0 0.0 0.0 1.472662334486217 5.042500717295727E-5 80-81 0.0 0.0 0.0 1.7124836686008018 5.042500717295727E-5 82-83 0.0 0.0 0.0 1.9803160941999645 5.042500717295727E-5 84-85 0.0 0.0 0.0 2.2568720460400487 5.042500717295727E-5 86-87 0.0 0.0 0.0 2.6123179216022243 5.042500717295727E-5 88-89 0.0 0.0 0.0 3.0251978803343986 5.042500717295727E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9155 0.0 23.263634 1 GAGTACT 7685 0.0 22.06641 12-13 TACATGG 9490 0.0 21.922113 2 ACATGGG 9390 0.0 21.548573 3 AGTACTT 8045 0.0 21.462765 12-13 GTACTTT 7990 0.0 20.984684 14-15 AGAGTAC 13375 0.0 19.79969 10-11 CATGGGG 3580 0.0 19.768682 4 TATAGCG 250 2.1750202E-7 18.999163 5 TACTTTT 8985 0.0 18.660835 14-15 GTATCAA 20210 0.0 18.206755 1 CATGGGA 5870 0.0 18.125265 4 GTACACG 315 6.7429937E-9 18.110424 1 TACCTGG 1695 0.0 17.654089 2 TATACGA 135 0.009405123 17.591818 2 ACTTTTT 9600 0.0 17.514853 16-17 GTACCTG 1835 0.0 17.098804 1 TAGCACC 650 0.0 16.806953 4 AGTACAT 7160 0.0 16.783478 2 GTATAGG 710 0.0 16.739386 1 >>END_MODULE