FastQCFastQC Report
Thu 26 May 2016
SRR937235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192858
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81510.37170669509836024No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT77290.352462402946292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57060.2602083673452636No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47470.21647548541674838No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43140.19672956479626133No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39090.17826051664084042No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36280.16544618940214095No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29560.13480125024055364No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25110.11450809856360968No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23320.10634523530479402No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22570.10292504120193831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG92250.027.2604031
TACATGG94800.025.6611522
ACATGGG95050.024.093483
GTGTAGC11550.021.4024931
CTCGCGC1159.060932E-820.64659560-61
GAGTACT72500.020.47515712-13
GTAAGCG2600.020.0939494-95
GTATCAA175450.020.0774171
AGAGTAC126400.019.7863910-11
CATGGGG39500.019.7261074
AGTACTT78950.019.5544912-13
GACCGGT1250.006029518319.0018167
CATGGGA56650.018.8702344
GTACTTT79050.018.8674514-15
ACCGTGG5050.018.8136798
CGATCGT1303.2784737E-718.27285424-25
CCCGCGA1303.2875323E-718.26805120-21
AGTACAT71500.017.7427022
TCTACGG2152.3903027E-517.679342
TCAACGC197250.017.6314774
Produced by FastQC (version 0.11.5)