FastQCFastQC Report
Thu 26 May 2016
SRR937235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192858
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101680.46368711517116024No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT89950.4101952794024966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76900.350683902012807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59090.26946569271699305No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39830.18163510815565803No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38840.17712045193988848No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28310.12910092673579412No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT27730.1264559766295857No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23390.10666445342106055No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23140.10552438872010865No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22040.10050810403592024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG93250.024.3054831
TACATGG94900.022.9732632
ACATGGG93200.022.6278483
GAGTACT93000.021.99002812-13
AGTACTT96350.021.86641112-13
TTATGCG1150.003722253720.6541739
GTACTTT99300.020.61599214-15
AGAGTAC151550.020.4531510-11
CATGGGG38950.019.8772534
CTGGTCG4350.019.6570749
GTATCAA213850.019.13061
TAACGCA1507.726194E-418.9992394
CGTAGAC1759.945068E-518.9992373
TACTTTT114800.018.3289714-15
CATGGGA57800.017.58584
TAGACCG1650.001464779317.2720365
ACTTTTT119550.017.2431416-17
GTGTAGC11650.017.1300261
CAACGCA240850.016.703715
ATCAACG241050.016.6898523
Produced by FastQC (version 0.11.5)