##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937235_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2192858 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22180505988076 33.0 31.0 34.0 31.0 34.0 2 32.414852671718826 34.0 31.0 34.0 31.0 34.0 3 32.49006410811826 34.0 31.0 34.0 31.0 34.0 4 36.11431611166797 37.0 35.0 37.0 35.0 37.0 5 36.04907613716894 37.0 35.0 37.0 35.0 37.0 6 36.090058270986994 37.0 35.0 37.0 35.0 37.0 7 36.03744519708982 37.0 35.0 37.0 35.0 37.0 8 36.015274586863356 37.0 35.0 37.0 35.0 37.0 9 37.714892163560066 39.0 37.0 39.0 35.0 39.0 10-11 37.629090438140544 39.0 37.0 39.0 35.0 39.0 12-13 37.527875722002975 39.0 37.0 39.0 35.0 39.0 14-15 38.746992509318886 40.0 38.0 41.0 35.0 41.0 16-17 38.710993826321626 40.0 38.0 41.0 35.0 41.0 18-19 38.70477431735206 40.0 38.0 41.0 34.5 41.0 20-21 38.60368865653864 40.0 38.0 41.0 34.0 41.0 22-23 38.55713115030704 40.0 38.0 41.0 34.0 41.0 24-25 38.592810159162156 40.0 38.0 41.0 34.0 41.0 26-27 38.44269350774195 40.0 38.0 41.0 34.0 41.0 28-29 38.30541170472507 40.0 38.0 41.0 34.0 41.0 30-31 38.35384028514386 40.0 38.0 41.0 34.0 41.0 32-33 38.26250491367887 40.0 38.0 41.0 34.0 41.0 34-35 38.12687255627131 40.0 38.0 41.0 34.0 41.0 36-37 37.95828298047571 40.0 37.0 41.0 33.5 41.0 38-39 37.85134855973347 40.0 37.0 41.0 33.0 41.0 40-41 37.68811842809703 40.0 37.0 41.0 33.0 41.0 42-43 37.49809358380707 40.0 37.0 41.0 33.0 41.0 44-45 37.206419886741415 40.0 37.0 41.0 32.0 41.0 46-47 36.8671580649545 40.0 36.5 41.0 31.0 41.0 48-49 36.55190326961436 39.0 36.0 41.0 30.5 41.0 50-51 36.315904632219684 39.0 36.0 40.5 30.0 41.0 52-53 36.099981166131144 39.0 35.5 40.0 30.0 41.0 54-55 35.905297105421326 39.0 35.0 40.0 29.0 41.0 56-57 35.6095365044157 38.5 35.0 40.0 28.0 41.0 58-59 35.35267377094185 38.0 35.0 40.0 28.0 41.0 60-61 35.00528374386303 38.0 34.0 40.0 27.5 41.0 62-63 34.652592871950674 38.0 34.0 40.0 26.5 41.0 64-65 34.25518524227287 37.0 34.0 39.5 26.0 41.0 66-67 33.80873430928952 36.5 33.0 39.0 26.0 40.5 68-69 33.35774614680932 36.0 32.5 39.0 25.0 40.0 70-71 32.90617928748692 35.5 32.0 38.5 23.5 40.0 72-73 32.41536524480837 35.0 31.5 38.0 22.5 40.0 74-75 31.925605533965268 35.0 31.0 37.0 21.5 39.0 76-77 30.592845501167883 33.5 29.5 35.5 20.5 38.5 78-79 31.260545370470865 34.0 31.0 36.0 21.0 38.5 80-81 31.045113500281367 34.0 31.0 36.0 20.0 37.5 82-83 30.629041187345464 34.0 30.5 35.5 18.5 37.0 84-85 30.15858391195417 34.0 30.0 35.0 17.0 36.5 86-87 29.764310548152228 34.0 30.0 35.0 10.0 36.0 88-89 29.28074480882939 34.0 29.0 35.0 7.0 36.0 90-91 28.694254484330493 33.5 29.0 35.0 2.0 35.0 92-93 28.168015895238085 33.0 28.0 35.0 2.0 35.0 94-95 27.63618756891691 33.0 27.0 35.0 2.0 35.0 96-97 26.912593519507418 32.5 25.5 34.0 2.0 35.0 98-99 25.865424710583174 32.0 24.5 34.0 2.0 35.0 100-101 22.784516370873078 29.0 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11296264892949637 1101 2 0.15724008781533882 1101 3 0.09669881949883319 1101 4 0.056393417500167686 1101 5 0.03701105451983011 1101 6 0.1266080817016757 1101 7 0.10451501398006968 1101 8 0.05692993294585591 1101 9 0.16827912739510253 1101 10-11 0.11804514588778403 1101 12-13 0.10464331328288523 1101 14-15 0.2780161134346244 1101 16-17 0.07985756700607993 1101 18-19 0.07786937100232194 1101 20-21 0.1603931906130569 1101 22-23 0.13450318066688283 1101 24-25 0.14159694047275195 1101 26-27 0.12502108618813423 1101 28-29 0.014351763008924934 1101 30-31 0.21442623445859255 1101 32-33 0.14564862443032212 1101 34-35 0.17188366132327815 1101 36-37 0.2868625615117324 1101 38-39 0.2386952275740981 1101 40-41 0.29663620564727466 1101 42-43 0.30303438012680317 1101 44-45 0.2564978612346067 1101 46-47 0.2615956280794478 1101 48-49 0.2675856708236921 1101 50-51 0.346204782240342 1101 52-53 0.38729980852030366 1101 54-55 0.32238939006568046 1101 56-57 0.49414761242986316 1101 58-59 0.47017196810784156 1101 60-61 0.37929505365637084 1101 62-63 0.43953117139357545 1101 64-65 0.4788714360184514 1101 66-67 0.34734645625703564 1101 68-69 0.40084720782706285 1101 70-71 0.45251479491940216 1101 72-73 0.6302708425605275 1101 74-75 0.49197149353415526 1101 76-77 0.5123055903410645 1101 78-79 0.6440855267330683 1101 80-81 0.5634850968127765 1101 82-83 0.4668645276489265 1101 84-85 0.4772307114333927 1101 86-87 0.5379698492336509 1101 88-89 0.7892386778348488 1101 90-91 0.8611043556440094 1101 92-93 0.9639490854202641 1101 94-95 1.1308723284328615 1101 96-97 1.2496788733920177 1101 98-99 1.6127108312534162 1101 100-101 2.2359418183864896 1102 1 0.20560386856274704 1102 2 0.21105680746871514 1102 3 0.16736061675425873 1102 4 0.15312433893228672 1102 5 0.2002755051348828 1102 6 0.21110678721631615 1102 7 0.19413968029796536 1102 8 0.1492425008560332 1102 9 0.18078130722795294 1102 10-11 0.20405883523812918 1102 12-13 0.18375196285965956 1102 14-15 0.34770453147961433 1102 16-17 0.3342490918159271 1102 18-19 0.33886075898574575 1102 20-21 0.28649903658941156 1102 22-23 0.3130323300625619 1102 24-25 0.22550876791048324 1102 26-27 0.3419530217455744 1102 28-29 0.27965670485769323 1102 30-31 0.3127467570819036 1102 32-33 0.3254362081023672 1102 34-35 0.29535358633702913 1102 36-37 0.39407680219210306 1102 38-39 0.40959328517534743 1102 40-41 0.5015589389564639 1102 42-43 0.5770309862947158 1102 44-45 0.5424760025414344 1102 46-47 0.6631183251698758 1102 48-49 0.6669427878102923 1102 50-51 0.5298934283293875 1102 52-53 0.43248271326495313 1102 54-55 0.47401856360720984 1102 56-57 0.6316212867561362 1102 58-59 0.7465873503499196 1102 60-61 0.7173083204544994 1102 62-63 0.6386194982209545 1102 64-65 0.7066413328620555 1102 66-67 0.6917198046144506 1102 68-69 0.6526028116742921 1102 70-71 0.748592269532736 1102 72-73 0.9344807705254397 1102 74-75 0.7771858549437347 1102 76-77 0.7575099580553939 1102 78-79 0.8509709480109215 1102 80-81 0.8217525321220407 1102 82-83 0.8246413328858928 1102 84-85 1.0285892503417315 1102 86-87 1.105620961954486 1102 88-89 1.2004088121125527 1102 90-91 1.3303142165371362 1102 92-93 1.2764432166394961 1102 94-95 1.4332117921269045 1102 96-97 1.6831470210035917 1102 98-99 2.0827666349745613 1102 100-101 2.7846116173052913 1103 1 0.18761319007726485 1103 2 0.26407476234872007 1103 3 0.12060003124662444 1103 4 0.09777457348391749 1103 5 0.10862444660457271 1103 6 0.093463169859902 1103 7 0.11145440169580212 1103 8 0.0965101155482202 1103 9 0.14029752455213185 1103 10-11 0.16378247917132427 1103 12-13 0.24038341609784908 1103 14-15 0.19824077222212821 1103 16-17 0.21154878603555716 1103 18-19 0.2529659934187265 1103 20-21 0.2882689133362817 1103 22-23 0.22812151123068247 1103 24-25 0.30509420517149977 1103 26-27 0.2677507550268601 1103 28-29 0.22295232500598416 1103 30-31 0.2625149465953669 1103 32-33 0.30951386338798414 1103 34-35 0.26991846095234706 1103 36-37 0.30417680063743546 1103 38-39 0.363092698898555 1103 40-41 0.360731409067796 1103 42-43 0.4210599842891867 1103 44-45 0.34834944858064887 1103 46-47 0.3751888150321534 1103 48-49 0.3884990560624004 1103 50-51 0.2876039090599889 1103 52-53 0.2821564152530627 1103 54-55 0.2974550497431707 1103 56-57 0.3722944536081343 1103 58-59 0.185786601813966 1103 60-61 0.24059676454218248 1103 62-63 0.21556515572238055 1103 64-65 0.33296730848309153 1103 66-67 0.4012714112910487 1103 68-69 0.45312366929945114 1103 70-71 0.5421196387987379 1103 72-73 0.3130937574767927 1103 74-75 0.4064458738126042 1103 76-77 0.42246852591989636 1103 78-79 0.4095190517553178 1103 80-81 0.5946411583523883 1103 82-83 0.5791631920501814 1103 84-85 0.6663313605617702 1103 86-87 0.7553504481992803 1103 88-89 0.8325056046507591 1103 90-91 1.0271404871376255 1103 92-93 1.0455008943288213 1103 94-95 1.1452777816091064 1103 96-97 1.3762028936313797 1103 98-99 1.7046227103649265 1103 100-101 2.3059623409254932 1104 1 0.15808045509542978 1104 2 0.18677284403712946 1104 3 0.18805955784014117 1104 4 0.1026791967799312 1104 5 0.013502211656522434 1104 6 0.09231228554794058 1104 7 0.14357716097836004 1104 8 0.09757995468102365 1104 9 0.10953029400489811 1104 10-11 0.05958874398506708 1104 12-13 0.08571959748850588 1104 14-15 0.11020688977740889 1104 16-17 0.15749710444431742 1104 18-19 0.19595969114030964 1104 20-21 0.29550481511037674 1104 22-23 0.16834684248948406 1104 24-25 0.13556426293382629 1104 26-27 0.13475497450832563 1104 28-29 0.18595295540489332 1104 30-31 0.2103635499239047 1104 32-33 0.23208428288125305 1104 34-35 0.12699304219041352 1104 36-37 0.3068254169674134 1104 38-39 0.21689510775802034 1104 40-41 0.3582209330695747 1104 42-43 0.30406485298744457 1104 44-45 0.22322659777477583 1104 46-47 0.38705808466754377 1104 48-49 0.5412986217115616 1104 50-51 0.49454272358499196 1104 52-53 0.4337394478567447 1104 54-55 0.4515695146183205 1104 56-57 0.3685366516731463 1104 58-59 0.33551724955098905 1104 60-61 0.5178822599784283 1104 62-63 0.5147563718790735 1104 64-65 0.3925309176152325 1104 66-67 0.5540229699673347 1104 68-69 0.6957799924058392 1104 70-71 0.6820219013064275 1104 72-73 0.7497695664958641 1104 74-75 0.7278224058658616 1104 76-77 0.6922677135007831 1104 78-79 0.5816605091657543 1104 80-81 0.6172810970745317 1104 82-83 0.4528785511166795 1104 84-85 0.5508535275943842 1104 86-87 0.7331712267291053 1104 88-89 0.824364734015969 1104 90-91 1.0170593435922868 1104 92-93 0.8928883313423945 1104 94-95 1.1058986988780184 1104 96-97 1.3895633731793389 1104 98-99 1.6787931413199928 1104 100-101 2.4220011818810647 1105 1 0.17046000164454256 1105 2 0.15302840950685948 1105 3 0.1117842499964965 1105 4 0.10750044266926295 1105 5 0.14416624857868499 1105 6 0.11748118669010665 1105 7 0.07842775572514427 1105 8 0.1389000509859173 1105 9 0.15798473683206282 1105 10-11 0.15910233270111718 1105 12-13 0.19141899428621656 1105 14-15 0.2941638693635227 1105 16-17 0.32641239270633804 1105 18-19 0.36212870823757726 1105 20-21 0.3363905620423324 1105 22-23 0.34941759031843844 1105 24-25 0.24321817832604609 1105 26-27 0.2885907413627038 1105 28-29 0.25841964189037725 1105 30-31 0.1990602853270147 1105 32-33 0.3821359697064608 1105 34-35 0.3578744359835824 1105 36-37 0.4010763373744197 1105 38-39 0.3111913599648659 1105 40-41 0.11970536411476473 1105 42-43 0.10121555110787739 1105 44-45 0.23842936166425943 1105 46-47 0.29069714580417383 1105 48-49 0.4658495743330775 1105 50-51 0.46931981008557244 1105 52-53 0.40016701696416135 1105 54-55 0.46554317587810345 1105 56-57 0.5380188553516021 1105 58-59 0.37828924945925024 1105 60-61 0.5433711629038598 1105 62-63 0.6385636257815221 1105 64-65 0.5783872245974635 1105 66-67 0.6696797162929329 1105 68-69 0.6434743827724319 1105 70-71 0.5940949277281149 1105 72-73 0.6283295327509961 1105 74-75 0.7043420338921926 1105 76-77 0.6955394263076471 1105 78-79 0.7051788398238337 1105 80-81 0.7570812599260748 1105 82-83 0.7468325535931015 1105 84-85 0.9628053477143759 1105 86-87 1.2554050277908786 1105 88-89 1.3969703094201158 1105 90-91 1.1888779032241779 1105 92-93 1.1050996679789087 1105 94-95 1.339118913474259 1105 96-97 1.714532364299238 1105 98-99 1.8891080440795314 1105 100-101 2.3304798145282355 1106 1 0.06465559117851427 1106 2 0.11553234086357378 1106 3 0.09318586261891681 1106 4 0.12714472851426706 1106 5 0.02361710406581352 1106 6 0.08203300985067585 1106 7 0.08545257668675532 1106 8 0.04274716201742734 1106 9 0.007323410362424454 1106 10-11 -0.12025753918285176 1106 12-13 -0.19697633620748434 1106 14-15 0.08976896574941406 1106 16-17 0.051359896732243726 1106 18-19 0.18722370742141692 1106 20-21 0.24087549291022725 1106 22-23 0.23544030492052315 1106 24-25 0.3140588898586856 1106 26-27 0.31751954962746964 1106 28-29 0.29497859680915184 1106 30-31 0.30888244998472913 1106 32-33 0.32120858031641575 1106 34-35 0.05829005673366083 1106 36-37 0.418189122918065 1106 38-39 0.5675680912017782 1106 40-41 0.40077058083983275 1106 42-43 0.3668172502799223 1106 44-45 0.46904617006987337 1106 46-47 0.42007122165006194 1106 48-49 0.5228014845214446 1106 50-51 0.5797871845522167 1106 52-53 0.5380364096323049 1106 54-55 0.42624667083520507 1106 56-57 0.36815240302311025 1106 58-59 0.38088149968198337 1106 60-61 0.5232591352670966 1106 62-63 0.3742110493219357 1106 64-65 0.4572688348464027 1106 66-67 0.38205850715867484 1106 68-69 0.6045471590047171 1106 70-71 0.6721603155093163 1106 72-73 0.8411184670930467 1106 74-75 0.7309139842950394 1106 76-77 0.5470093461851313 1106 78-79 0.6722945487536798 1106 80-81 0.6554923940934216 1106 82-83 0.7612785371321529 1106 84-85 0.7144197850255622 1106 86-87 0.9260579688152006 1106 88-89 1.0920003790010533 1106 90-91 1.083621546163311 1106 92-93 1.2133783064590595 1106 94-95 1.0018802332277197 1106 96-97 1.3685032030328657 1106 98-99 1.7107256264760977 1106 100-101 2.284310200313058 1107 1 0.08385809659074539 1107 2 0.12922449195588115 1107 3 0.08673392895773446 1107 4 0.09052344140714297 1107 5 0.054146473142239415 1107 6 0.11532736874809757 1107 7 0.06576161881403664 1107 8 0.02971071474151188 1107 9 0.09059866727969279 1107 10-11 0.07728124917593249 1107 12-13 0.1156556985982391 1107 14-15 0.10787605110125043 1107 16-17 0.14313673414212502 1107 18-19 0.0386246937464918 1107 20-21 0.20766813960732833 1107 22-23 0.14857121541335516 1107 24-25 0.11019791302354065 1107 26-27 0.058589264877134895 1107 28-29 0.16541396495403404 1107 30-31 0.22984920643360596 1107 32-33 0.1660188383414294 1107 34-35 0.11497082165021766 1107 36-37 0.14776167031976684 1107 38-39 0.1629384341128457 1107 40-41 0.22100066811582053 1107 42-43 0.22063902008274283 1107 44-45 0.15036528391244275 1107 46-47 0.19005775867912433 1107 48-49 0.2775787240323666 1107 50-51 0.3460514521613618 1107 52-53 0.3538496001165541 1107 54-55 0.37304307687178806 1107 56-57 0.2874977807800363 1107 58-59 0.4898162110327533 1107 60-61 0.5725464962107836 1107 62-63 0.49656370240380454 1107 64-65 0.7148761548298666 1107 66-67 0.655167541959166 1107 68-69 0.6397041790260332 1107 70-71 0.7281396932208821 1107 72-73 0.7773714959344247 1107 74-75 0.6483040257960546 1107 76-77 0.5960950024297311 1107 78-79 0.7085389206474311 1107 80-81 0.7287952616582558 1107 82-83 0.6615960748433025 1107 84-85 0.7870607340886941 1107 86-87 0.9608721998776346 1107 88-89 0.9863765685136308 1107 90-91 0.9987290536776321 1107 92-93 0.9513173467197547 1107 94-95 0.9817588266241906 1107 96-97 1.2128608793249498 1107 98-99 1.6603518949260803 1107 100-101 2.110348837774918 1108 1 0.06780430239078328 1108 2 0.12703628230478614 1108 3 0.10262685533484728 1108 4 0.08832830833076599 1108 5 0.0317882893277428 1108 6 0.08462197759900647 1108 7 0.059126126566646064 1108 8 0.07097595849494809 1108 9 0.10444918319705465 1108 10-11 0.08931477321569758 1108 12-13 0.15587631965392745 1108 14-15 0.16777976661851568 1108 16-17 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92-93 0.7996393211105008 2316 94-95 0.9475870951476288 2316 96-97 1.1106697343828777 2316 98-99 1.3166069351495224 2316 100-101 1.7280473979920679 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 14.0 5 39.0 6 69.0 7 170.0 8 414.0 9 803.0 10 1485.0 11 2437.0 12 3952.0 13 5858.0 14 7650.0 15 10620.0 16 14923.0 17 20803.0 18 18331.0 19 11122.0 20 9756.0 21 10052.0 22 10705.0 23 11948.0 24 13970.0 25 17082.0 26 20654.0 27 26221.0 28 32844.0 29 40990.0 30 52078.0 31 66430.0 32 86176.0 33 113940.0 34 157077.0 35 225259.0 36 340601.0 37 488733.0 38 345897.0 39 23750.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.25876693749011 20.288262954063423 15.662670389975716 21.790299718470756 2 15.098834489054925 21.488714727538216 40.38410147852711 23.02834930487975 3 19.492780654287692 25.22379470079686 30.65419648695903 24.62922815795642 4 12.439245952086273 17.789432785889463 38.694616796892454 31.076704465131805 5 12.902041599953304 38.48353663903278 34.82531093253996 13.789110828473955 6 30.511369181223774 37.986545412425244 17.600501263647715 13.901584142703266 7 27.615376827865735 33.579009675957124 21.176473807241507 17.629139688935627 8 25.742341729377827 35.33730866294124 19.963080144724373 18.95726946295656 9 25.662674008075303 17.4561690725072 21.102415204267672 35.77874171514982 10-11 24.303010044426042 27.50367328846647 27.872598225694507 20.32071844141299 12-13 25.733498576544513 24.77519436409677 28.382049297051275 21.109257762307447 14-15 22.156199808651543 26.238999515700517 27.435930643935908 24.16887003171204 16-17 21.033646501506254 29.749235928637425 27.852168266253447 21.364949303602877 18-19 20.87437946278327 28.23465997342281 30.527033670214855 20.363926893579066 20-21 21.83186135712452 26.87767601808964 30.53582609353916 20.75463653124668 22-23 21.35133951922599 27.004239709344823 30.49779304362974 21.14662772779944 24-25 21.615859671684092 27.138995579968146 30.54781261436277 20.697332133984993 26-27 21.465752865547238 27.163830476621754 30.727144084200308 20.643272573630693 28-29 20.81788963996575 27.876882173407925 30.640705680352887 20.664522506273435 30-31 21.144890498965733 27.143825221537575 30.570908440864336 21.14037583863236 32-33 21.106812303224654 27.319258537724316 30.445341600178033 21.128587558872994 34-35 21.740007788922036 27.194145722158027 30.272525626374346 20.793320862545592 36-37 21.595424783547315 26.89626961709331 30.547372424479835 20.96093317487954 38-39 21.22123758611766 27.381647006246418 30.63589403324201 20.761221374393912 40-41 21.37721639978512 27.470064181082403 30.311903461145228 20.840815957987246 42-43 21.21198646583417 27.49625685179687 29.858305365186695 21.43345131718227 44-45 21.471454147965805 27.335262930841854 29.71966265029473 21.473620270897616 46-47 21.75416739250786 27.867513537128257 29.362982007954912 21.015337062408964 48-49 21.811147826261436 27.80467317081179 29.242727983298504 21.141451019628267 50-51 21.41846394066556 27.971419033973017 29.31192991064629 21.298187114715134 52-53 21.84519513921903 28.363106129787276 28.599806416969635 21.191892314024052 54-55 21.397714763108237 27.90146466392261 28.93495611662953 21.765864456339624 56-57 21.75797520860904 28.0718815354209 28.74791710179136 21.422226154178702 58-59 22.28306620857347 27.690256277424258 28.734099517615824 21.29257799638645 60-61 21.81630091870974 27.74666667882736 29.521929828561632 20.915102573901272 62-63 21.527340119606468 27.942005364688455 29.343920126155 21.186734389550075 64-65 21.376691970022684 28.155311470236562 29.002197132691677 21.465799427049085 66-67 21.517398755414167 28.091969475451673 28.660291728876196 21.730340040257964 68-69 21.469607243150264 27.90796303271803 28.96345773415333 21.658971989978376 70-71 21.723203235230006 27.96323336942018 28.851891002518176 21.461672392831638 72-73 21.709472802877897 27.647882220934704 29.184762397271314 21.45788257891608 74-75 21.435838526708068 28.387907470524766 28.60561422581854 21.57063977694862 76-77 21.5593531364092 27.80417154234337 28.852347028398555 21.784128292848877 78-79 21.573398733524925 27.9722398805577 28.774640218381677 21.6797211675357 80-81 21.64939544649038 28.184953152461308 28.63299857993541 21.532652821112904 82-83 21.59051680304189 28.02965733587432 28.879944656421536 21.49988120466225 84-85 21.434830402332306 28.12288018591318 28.675950013030977 21.766339398723535 86-87 21.38056819000592 28.060480888411377 28.759636966917146 21.799313954665557 88-89 21.389187079145117 27.946702431256377 28.47799538319399 22.186115106404518 90-91 21.347310994524186 27.670939366969016 28.828766581312998 22.1529830571938 92-93 21.635763579641402 27.645094930142562 28.927095346956865 21.79204614325917 94-95 21.360320640917013 27.5555690336538 28.95321995313878 22.130890372290406 96-97 21.104604128493502 27.572350786051807 29.247470652454467 22.075574433000224 98-99 21.15767985201004 27.83599345418511 28.54835359348319 22.45797310032166 100-101 20.976652852654667 27.4093243098714 29.2617534453125 22.35226939216143 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 313.0 1 223.0 2 196.5 3 322.5 4 297.5 5 253.0 6 528.5 7 1274.5 8 2202.0 9 2693.0 10 2506.5 11 2225.5 12 2345.5 13 2597.5 14 2832.0 15 2993.5 16 3142.0 17 3318.0 18 3521.5 19 3812.5 20 4291.5 21 4998.0 22 5904.0 23 7180.0 24 8875.0 25 11122.0 26 14160.0 27 17754.0 28 21020.5 29 24779.0 30 29946.0 31 35196.5 32 40455.0 33 45849.5 34 52444.0 35 57887.0 36 62822.0 37 68954.0 38 72732.0 39 74206.0 40 75100.0 41 77243.5 42 80423.0 43 83039.5 44 84410.0 45 85224.5 46 83820.5 47 87733.0 48 102184.0 49 99916.0 50 83165.0 51 74333.0 52 68501.5 53 64082.0 54 58835.5 55 51411.5 56 44190.5 57 37573.5 58 32431.5 59 28161.0 60 23669.0 61 18654.0 62 13984.0 63 10197.5 64 7149.5 65 5289.0 66 3876.5 67 2849.5 68 1986.0 69 1294.0 70 949.0 71 721.5 72 608.5 73 520.0 74 409.0 75 355.5 76 248.5 77 127.5 78 78.0 79 46.5 80 21.5 81 12.5 82 6.5 83 3.5 84 2.0 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04108793182230678 2 0.0 3 0.0 4 0.0 5 8.664491727234505E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005951137738968962 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.5602588038076337E-5 22-23 8.664491727234505E-4 24-25 2.2801294019038168E-5 26-27 0.0018013022275040153 28-29 0.0036710083370651452 30-31 9.120517607615267E-5 32-33 9.120517607615267E-5 34-35 0.0 36-37 0.0 38-39 2.2801294019038168E-5 40-41 0.0 42-43 0.008436478787044122 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.2801294019038168E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.964168222474962E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.016284684188397E-4 84-85 2.964168222474962E-4 86-87 0.0 88-89 0.0 90-91 0.009872960310243527 92-93 0.0015960905813326715 94-95 0.0 96-97 0.0 98-99 2.964168222474962E-4 100-101 0.012654718180566182 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2192858.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.40473214134818 #Duplication Level Percentage of deduplicated Percentage of total 1 74.1798628965835 38.8737584537739 2 14.318267701151663 15.0068996721394 3 5.0468604118125615 7.934381041074342 4 2.258079847755413 4.733362783015948 5 1.1762104019995887 3.0819495529327954 6 0.706417171363306 2.2211761587205743 7 0.45749042081789587 1.6782264072136142 8 0.3209383448880017 1.3454950398194678 9 0.21724187639299125 1.024605210803272 >10 1.1608185571576108 11.067372843213182 >50 0.0943380670420184 3.4483314950885773 >100 0.058849627474625375 5.519646936783006 >500 0.0028795149717948305 1.0312428950982524 >1k 0.0013961284711732145 1.5354325084962945 >5k 2.617740883449777E-4 1.033163443235609 >10k+ 8.725802944832591E-5 0.46495555859164694 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10168 0.46368711517116024 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8995 0.4101952794024966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7690 0.350683902012807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5909 0.26946569271699305 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3983 0.18163510815565803 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3884 0.17712045193988848 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2831 0.12910092673579412 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2773 0.1264559766295857 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2339 0.10666445342106055 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2314 0.10552438872010865 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2204 0.10050810403592024 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.5602588038076337E-5 0.0 9 0.0 0.0 0.0 4.5602588038076337E-5 0.0 10-11 0.0 0.0 0.0 4.5602588038076337E-5 0.0 12-13 0.0 0.0 0.0 4.5602588038076337E-5 0.0 14-15 0.0 0.0 0.0 4.5602588038076337E-5 0.0 16-17 0.0 0.0 0.0 4.5602588038076337E-5 0.0 18-19 0.0 0.0 0.0 4.5602588038076337E-5 0.0 20-21 0.0 0.0 0.0 4.5602588038076337E-5 0.0 22-23 0.0 0.0 0.0 1.1400647009519085E-4 0.0 24-25 0.0 0.0 0.0 3.876219983236489E-4 0.0 26-27 0.0 0.0 0.0 5.928336444949924E-4 0.0 28-29 0.0 0.0 0.0 0.0010716608188947939 0.0 30-31 0.0 0.0 0.0 0.002280129401903817 0.0 32-33 0.0 0.0 0.0 0.003921822571274565 0.0 34-35 0.0 0.0 0.0 0.006065144209064153 0.0 36-37 0.0 0.0 0.0 0.009371331841824686 0.0 38-39 0.0 0.0 0.0 0.015254065698736535 0.0 40-41 0.0 0.0 0.0 0.020840382733400886 0.0 42-43 0.0 0.0 0.0 0.027293148940788686 0.0 44-45 0.0 0.0 0.0 0.03246904268311035 0.0 46-47 0.0 0.0 0.0 0.04017588006154525 0.0 48-49 0.0 0.0 0.0 0.04662864626893305 0.0 50-51 0.0 0.0 0.0 0.05392506035502527 0.0 52-53 0.0 0.0 0.0 0.0721660955702558 0.0 54-55 0.0 0.0 0.0 0.10349507355241425 0.0 56-57 0.0 0.0 0.0 0.14959929005890943 0.0 58-59 0.0 0.0 0.0 0.2016774455983926 0.0 60-61 0.0 0.0 0.0 0.268257224133984 0.0 62-63 0.0 0.0 0.0 0.311237663359871 0.0 64-65 0.0 0.0 0.0 0.3676252634689524 0.0 66-67 0.0 0.0 0.0 0.4685665920912344 0.0 68-69 0.0 0.0 0.0 0.6055795678516347 0.0 70-71 0.0 0.0 0.0 0.7479508477065091 0.0 72-73 0.0 0.0 0.0 0.90984003524168 0.0 74-75 0.0 0.0 0.0 1.0798692847416476 0.0 76-77 0.0 0.0 0.0 1.2416900683947616 0.0 78-79 0.0 0.0 0.0 1.4091655729645969 0.0 80-81 0.0 0.0 0.0 1.5874260896054373 0.0 82-83 0.0 0.0 0.0 1.802966721967405 0.0 84-85 0.0 0.0 0.0 2.1086408695866306 0.0 86-87 0.0 0.0 0.0 2.4806439815072387 0.0 88-89 0.0 0.0 0.0 2.899138931932665 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9325 0.0 24.305483 1 TACATGG 9490 0.0 22.973263 2 ACATGGG 9320 0.0 22.627848 3 GAGTACT 9300 0.0 21.990028 12-13 AGTACTT 9635 0.0 21.866411 12-13 TTATGCG 115 0.0037222537 20.654173 9 GTACTTT 9930 0.0 20.615992 14-15 AGAGTAC 15155 0.0 20.45315 10-11 CATGGGG 3895 0.0 19.877253 4 CTGGTCG 435 0.0 19.657074 9 GTATCAA 21385 0.0 19.1306 1 TAACGCA 150 7.726194E-4 18.999239 4 CGTAGAC 175 9.945068E-5 18.999237 3 TACTTTT 11480 0.0 18.32897 14-15 CATGGGA 5780 0.0 17.5858 4 TAGACCG 165 0.0014647793 17.272036 5 ACTTTTT 11955 0.0 17.24314 16-17 GTGTAGC 1165 0.0 17.130026 1 CAACGCA 24085 0.0 16.70371 5 ATCAACG 24105 0.0 16.689852 3 >>END_MODULE