FastQCFastQC Report
Thu 26 May 2016
SRR937234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2149263
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83480.3884122138612166No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76720.35695957172295806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57760.26874328548902576No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47820.22249487382418995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43780.20369773266463898No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38210.177781872204565No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30220.14060633807961148No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30160.14032717261684588No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25300.11771477013283159No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23040.10719953770199366No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22670.1054780173482724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG92350.026.0526071
TACATGG95750.024.8237512
ACATGGG96100.023.5450423
GAGTACT70450.022.58710312-13
GTAGGAC5450.020.932963
GTACTTT76950.020.6167114-15
AGTACTT74300.020.23399512-13
GTGTAGC10400.019.6595081
GTATCAA181750.019.4118561
CATGGGA59550.019.319244
AGAGTAC128700.019.21051210-11
GCGTATC1250.005997295519.0194321
CATGGGG37500.018.00174
GAGTGCG1350.00940195317.5929269
GTGTAAG10550.017.57721
ATGGGAT28550.017.4835455
ATCAACG201750.017.2705553
CAACGCA202050.017.2461225
TCAACGC202300.017.225214
TATCAAC205800.016.977672
Produced by FastQC (version 0.11.5)