##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937234_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2149263 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77850500380828 33.0 31.0 34.0 30.0 34.0 2 31.89439077488423 33.0 31.0 34.0 30.0 34.0 3 31.94032000736997 33.0 31.0 34.0 30.0 34.0 4 35.55454590713189 37.0 35.0 37.0 33.0 37.0 5 35.57204911637152 37.0 35.0 37.0 33.0 37.0 6 35.62220165703313 37.0 35.0 37.0 33.0 37.0 7 35.566235495609426 37.0 35.0 37.0 33.0 37.0 8 35.55781633052818 37.0 35.0 37.0 33.0 37.0 9 37.19100454434846 39.0 37.0 39.0 34.0 39.0 10-11 37.07279053331305 39.0 37.0 39.0 33.0 39.0 12-13 36.955551507656345 39.0 37.0 39.0 33.0 39.0 14-15 38.137736749760265 40.0 38.0 41.0 33.0 41.0 16-17 38.11164594561019 40.0 38.0 41.0 33.0 41.0 18-19 38.088853248764806 40.0 38.0 41.0 33.5 41.0 20-21 38.03141774645541 40.0 38.0 41.0 33.0 41.0 22-23 37.88653180183161 40.0 38.0 41.0 33.0 41.0 24-25 37.72297480578226 40.0 37.0 41.0 32.5 41.0 26-27 37.575351178520265 40.0 37.0 41.0 32.0 41.0 28-29 37.6572776342402 40.0 37.0 41.0 33.0 41.0 30-31 37.528871524797104 40.0 37.0 41.0 32.0 41.0 32-33 37.35390224462991 40.0 37.0 41.0 32.0 41.0 34-35 37.231078048614805 39.5 36.5 41.0 31.5 41.0 36-37 37.235374405086766 40.0 37.0 41.0 31.5 41.0 38-39 37.15684609096235 40.0 37.0 41.0 31.0 41.0 40-41 37.00787107022268 40.0 36.5 41.0 31.0 41.0 42-43 36.790580538538094 39.0 36.0 41.0 31.0 41.0 44-45 36.582843048989346 39.0 36.0 41.0 30.0 41.0 46-47 36.26966104194787 39.0 36.0 41.0 30.0 41.0 48-49 35.9775039164588 39.0 35.5 41.0 29.0 41.0 50-51 35.12527712988127 38.0 34.0 40.0 27.0 40.5 52-53 35.24240635045595 38.5 35.0 40.0 28.0 40.5 54-55 35.590787632783886 39.0 35.0 40.0 28.0 41.0 56-57 35.41211708385619 39.0 35.0 40.0 27.0 41.0 58-59 35.19190159603548 38.5 35.0 40.0 26.5 41.0 60-61 34.91471588167665 38.0 34.0 40.0 26.0 41.0 62-63 34.61313994611176 38.0 34.0 40.0 26.0 41.0 64-65 34.26957357010287 37.5 34.0 40.0 25.5 41.0 66-67 33.89811298105444 37.0 33.5 40.0 24.5 41.0 68-69 33.43264900572894 36.5 33.0 39.0 22.5 41.0 70-71 32.99343775052192 36.0 32.5 39.0 22.0 40.0 72-73 32.602533286991864 35.5 32.0 38.5 21.5 40.0 74-75 32.14531516152282 35.0 32.0 37.5 20.0 39.5 76-77 31.701232468990533 35.0 31.0 37.0 18.5 39.0 78-79 31.214419082262154 35.0 31.0 36.5 17.5 39.0 80-81 30.705263618272866 34.5 30.5 36.0 12.0 38.5 82-83 30.214486779886876 34.0 30.0 35.5 7.5 37.0 84-85 29.768104927130835 34.0 29.5 35.0 6.5 37.0 86-87 29.339980728277553 34.0 29.0 35.0 2.0 36.0 88-89 28.9094571022718 34.0 29.0 35.0 2.0 36.0 90-91 28.47832954831493 34.0 29.0 35.0 2.0 35.5 92-93 28.026467212249038 34.0 27.5 35.0 2.0 35.0 94-95 27.490205247100985 33.0 26.5 35.0 2.0 35.0 96-97 26.85054411675072 33.0 25.5 35.0 2.0 35.0 98-99 26.0751173774452 32.5 24.5 34.5 2.0 35.0 100-101 24.092589878483928 30.5 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0354105315642137 1101 2 0.04711516487918033 1101 3 0.13127897853699722 1101 4 -0.0127285380435751 1101 5 0.0829447550801703 1101 6 0.004933636553040799 1101 7 -0.026422412310502352 1101 8 0.0019244591227050023 1101 9 0.10353050265477037 1101 10-11 0.14518610782942432 1101 12-13 -0.011475823506849281 1101 14-15 0.07645648480615108 1101 16-17 0.2738685068657105 1101 18-19 0.2345835465253714 1101 20-21 0.27448624466266125 1101 22-23 0.23773458906914158 1101 24-25 0.17306365906265597 1101 26-27 0.19755045265880966 1101 28-29 0.12448468164509308 1101 30-31 0.17985793983720555 1101 32-33 0.13246917075282028 1101 34-35 0.20424545192435772 1101 36-37 0.2568765195437024 1101 38-39 0.2518647874466282 1101 40-41 0.20604770622474433 1101 42-43 0.28431494644588895 1101 44-45 0.35491422808267714 1101 46-47 0.357591176308226 1101 48-49 0.3375889631200977 1101 50-51 0.08799337429156395 1101 52-53 0.2917676476453508 1101 54-55 0.29280690032097567 1101 56-57 0.39040688056152817 1101 58-59 0.5294106781165269 1101 60-61 0.43735560542531005 1101 62-63 0.47616213893926584 1101 64-65 0.441154446328774 1101 66-67 0.5342041599316261 1101 68-69 0.7534324262366567 1101 70-71 0.6897362966202252 1101 72-73 0.6307911683544702 1101 74-75 0.5705810572776784 1101 76-77 0.6807695014646669 1101 78-79 0.6434734776907156 1101 80-81 0.5645137342815509 1101 82-83 0.49759649164067454 1101 84-85 0.678398705293688 1101 86-87 0.6163496653115708 1101 88-89 0.5866150114213404 1101 90-91 0.8471915940650412 1101 92-93 1.0244713693161174 1101 94-95 1.140995615605629 1101 96-97 1.1680114274732887 1101 98-99 1.2655303704474754 1101 100-101 1.7071528006121213 1102 1 0.09133198919587926 1102 2 0.15711247675861983 1102 3 0.16578524671430017 1102 4 0.04251815344773746 1102 5 0.08021815951303779 1102 6 -0.04657926916164712 1102 7 0.1296671041561197 1102 8 0.07992185034616739 1102 9 0.16212007384140037 1102 10-11 0.2850483194207598 1102 12-13 0.22022742918743177 1102 14-15 0.35427545459250354 1102 16-17 0.3189942192518558 1102 18-19 0.39375516763306706 1102 20-21 0.36547861258907943 1102 22-23 0.547324852463916 1102 24-25 0.4194275143063422 1102 26-27 0.3403831058679998 1102 28-29 0.259497674227795 1102 30-31 0.2640828958559922 1102 32-33 0.2908837747030546 1102 34-35 0.3572598747513567 1102 36-37 0.4050797708699676 1102 38-39 0.47690151825427307 1102 40-41 0.4838062274985191 1102 42-43 0.4571691353995533 1102 44-45 0.32704361628558587 1102 46-47 0.32607330475251217 1102 48-49 0.12307446300108182 1102 50-51 -0.014505244028150344 1102 52-53 0.2024297050630608 1102 54-55 0.14443418795945462 1102 56-57 0.10171230851305779 1102 58-59 0.15790848384406786 1102 60-61 0.21059553625921978 1102 62-63 0.10007808421378428 1102 64-65 0.15779259588951078 1102 66-67 0.2817936579302369 1102 68-69 0.22769025188228653 1102 70-71 0.2838452256948756 1102 72-73 0.20656256661491312 1102 74-75 0.31329936276308246 1102 76-77 0.44348566260962485 1102 78-79 0.3547862586437738 1102 80-81 0.4065604029272585 1102 82-83 0.5937313436367262 1102 84-85 0.6506407825200391 1102 86-87 0.5301205888759242 1102 88-89 0.7109224612772884 1102 90-91 0.8277542765220822 1102 92-93 0.8889989851378601 1102 94-95 0.8549677891118392 1102 96-97 1.0188053464384232 1102 98-99 1.1286065379673182 1102 100-101 1.621975459895232 1103 1 -0.044241646361928844 1103 2 -0.057135310087936375 1103 3 -0.0137802096730546 1103 4 -0.17637682393147713 1103 5 -0.13702270871079492 1103 6 -0.05896386285084532 1103 7 -0.06229877471617584 1103 8 0.06133380770626928 1103 9 0.04210398602899801 1103 10-11 0.015277793789621796 1103 12-13 0.028199314368229977 1103 14-15 0.03833700846220722 1103 16-17 -0.05225795361010199 1103 18-19 -0.018146072193331975 1103 20-21 -0.11785582396336025 1103 22-23 -0.1759108124030675 1103 24-25 -0.10387966793778247 1103 26-27 0.13095698605264516 1103 28-29 0.024659861824936513 1103 30-31 -0.16467205424012832 1103 32-33 -0.05502361820794732 1103 34-35 -0.03392075195383626 1103 36-37 -0.030950669343226878 1103 38-39 0.05125944473959265 1103 40-41 0.08618004321679962 1103 42-43 0.07104221148138379 1103 44-45 -0.03120807883453125 1103 46-47 -0.07555864408882229 1103 48-49 -0.11294619213838786 1103 50-51 -0.12029124446559791 1103 52-53 -0.06532013089305622 1103 54-55 -0.11786370709851468 1103 56-57 -0.12519203951323732 1103 58-59 -0.20499040180871475 1103 60-61 -0.30239702708474425 1103 62-63 -0.2653249888749585 1103 64-65 -0.079386439302219 1103 66-67 -0.05202737019321546 1103 68-69 -0.0027834895845586516 1103 70-71 0.013663870865080696 1103 72-73 0.05876442405214988 1103 74-75 0.2027776744349481 1103 76-77 0.247022607018895 1103 78-79 0.26827541447971015 1103 80-81 0.1499299362367985 1103 82-83 0.04727670608737 1103 84-85 0.0400111705845525 1103 86-87 0.2260584162442001 1103 88-89 0.29655624625892685 1103 90-91 0.30627711891332154 1103 92-93 0.33388167937157576 1103 94-95 0.3376935365187954 1103 96-97 0.4874729647514755 1103 98-99 0.5569323118515292 1103 100-101 0.7082459941602615 1104 1 0.18487641433084434 1104 2 0.0531335232873964 1104 3 0.18437770859574698 1104 4 0.06621339011094562 1104 5 0.030437234547228798 1104 6 0.08365724223560989 1104 7 0.13001626515765707 1104 8 0.11506186896808401 1104 9 0.19260027503910493 1104 10-11 0.2665540816616314 1104 12-13 0.2777125771941087 1104 14-15 0.3914368840851097 1104 16-17 0.41482174528351123 1104 18-19 0.4339535026483432 1104 20-21 0.3788965518018088 1104 22-23 0.5019250667316584 1104 24-25 0.3036311236045819 1104 26-27 0.31158626913654786 1104 28-29 0.2747726547817777 1104 30-31 0.3515579377119451 1104 32-33 0.1971631425409086 1104 34-35 0.34525665206037104 1104 36-37 0.255385937356877 1104 38-39 0.43042262261509734 1104 40-41 0.5294045398147205 1104 42-43 0.535353910244929 1104 44-45 0.5205659206614541 1104 46-47 0.5684617033541386 1104 48-49 0.646653403026356 1104 50-51 0.4887165433291969 1104 52-53 0.5183061931539967 1104 54-55 0.5768260817855904 1104 56-57 0.6401417057006853 1104 58-59 0.6224160511678036 1104 60-61 0.46762321467832635 1104 62-63 0.442701805041132 1104 64-65 0.4948414878274363 1104 66-67 0.595321893705723 1104 68-69 0.5718905286086411 1104 70-71 0.7149718627033437 1104 72-73 0.7444531317497081 1104 74-75 0.6325798091686821 1104 76-77 0.6934022579500976 1104 78-79 0.9021769126953423 1104 80-81 0.860400577551129 1104 82-83 0.7871954545123323 1104 84-85 0.9119525507638819 1104 86-87 1.1024485132258164 1104 88-89 0.7918555235710834 1104 90-91 0.7773960250451921 1104 92-93 0.9990136166464971 1104 94-95 1.1731093055838535 1104 96-97 1.1965575736952445 1104 98-99 1.3562472216578136 1104 100-101 1.627253538078385 1105 1 0.15648858874845217 1105 2 0.1450039756400514 1105 3 0.20211075007000545 1105 4 0.2584018223515372 1105 5 0.20848997159357907 1105 6 0.15093191652068327 1105 7 0.24118835695254148 1105 8 0.2493010449770381 1105 9 0.2883225346915097 1105 10-11 0.31383746930890055 1105 12-13 0.2255825746013045 1105 14-15 0.24868261342918885 1105 16-17 0.3208118926299335 1105 18-19 0.3256343622639335 1105 20-21 0.33782201303650083 1105 22-23 0.3849367092871816 1105 24-25 0.38598232414974376 1105 26-27 0.3398378038545715 1105 28-29 0.2872241842949137 1105 30-31 0.2287780860125963 1105 32-33 0.14471487089992507 1105 34-35 0.17406636244710683 1105 36-37 0.15137451359927923 1105 38-39 0.3803690797755266 1105 40-41 0.21994996351642015 1105 42-43 0.3593783076590711 1105 44-45 0.2390682247867133 1105 46-47 0.4088753181753333 1105 48-49 0.39789269566327334 1105 50-51 0.3127039282313646 1105 52-53 0.4224520764277102 1105 54-55 0.5179660671540844 1105 56-57 0.4102134270812883 1105 58-59 0.4515606091237174 1105 60-61 0.6136715988990389 1105 62-63 0.5861099634084113 1105 64-65 0.5954743632273178 1105 66-67 0.6278087586013825 1105 68-69 0.5789486411440308 1105 70-71 0.6296645769253075 1105 72-73 0.7608550722077538 1105 74-75 0.6620969019129745 1105 76-77 0.6540421503053722 1105 78-79 0.6084495696057424 1105 80-81 0.6429837810033199 1105 82-83 0.7378029320036177 1105 84-85 0.8071564424752999 1105 86-87 0.7960741682942682 1105 88-89 0.7813922599350072 1105 90-91 1.0435680349113419 1105 92-93 0.9866499918492693 1105 94-95 1.079548326024593 1105 96-97 1.1217695522549747 1105 98-99 1.2542217504952795 1105 100-101 1.5046096880831534 1106 1 0.0710495296914111 1106 2 0.06113743008152639 1106 3 0.018200471016665887 1106 4 -0.006196334113447222 1106 5 0.024956263161421077 1106 6 0.07905781011372426 1106 7 0.08735494792662024 1106 8 0.04691042076549934 1106 9 0.13406335486274656 1106 10-11 -0.1507422896522641 1106 12-13 -0.21312682473596567 1106 14-15 -0.0708503843336743 1106 16-17 -0.10714243109885757 1106 18-19 0.059622856990692696 1106 20-21 0.13871465992217225 1106 22-23 0.1429734748588274 1106 24-25 0.05986091744505728 1106 26-27 0.1771410031279288 1106 28-29 0.2598745602347634 1106 30-31 0.04470685508292149 1106 32-33 0.08684652133472781 1106 34-35 -0.0347993420826711 1106 36-37 0.08368116109048884 1106 38-39 -0.023215174484285228 1106 40-41 0.0871928400029347 1106 42-43 0.1042855632140629 1106 44-45 0.24314942190702737 1106 46-47 0.3727850926114229 1106 48-49 0.4233343408837058 1106 50-51 0.2279633435481756 1106 52-53 0.08051575491924723 1106 54-55 0.17190902017421905 1106 56-57 0.11559243668583719 1106 58-59 0.2396959012129969 1106 60-61 0.15602591169445645 1106 62-63 0.15548663495304993 1106 64-65 0.2358363840511899 1106 66-67 0.48067593072632064 1106 68-69 0.21822867848570837 1106 70-71 0.31269040912324897 1106 72-73 0.428942656941409 1106 74-75 0.2652534930552619 1106 76-77 0.4519364245816675 1106 78-79 0.41300016589512367 1106 80-81 0.5604938943739946 1106 82-83 0.4384117316873919 1106 84-85 0.3316974510369697 1106 86-87 0.6438723550362901 1106 88-89 0.6535616725605315 1106 90-91 0.6953647876993507 1106 92-93 0.9459087721893837 1106 94-95 1.0664828393212566 1106 96-97 1.0919764243008636 1106 98-99 1.3488120433414927 1106 100-101 1.2560018009155698 1107 1 0.02672871600606541 1107 2 -0.019779743010992945 1107 3 0.1070702820288787 1107 4 0.034941380345806294 1107 5 0.026568774800040273 1107 6 0.07874598694728974 1107 7 0.1492048122678966 1107 8 0.10933136994270853 1107 9 0.03079982820322158 1107 10-11 0.14005158501443304 1107 12-13 0.14838259962386502 1107 14-15 0.28627067647413895 1107 16-17 0.2878044484168498 1107 18-19 0.14644360393127442 1107 20-21 -0.05018771001731892 1107 22-23 0.22360938136944952 1107 24-25 0.10050524213828993 1107 26-27 0.26322526337908414 1107 28-29 0.26294544483641147 1107 30-31 0.2649133508496391 1107 32-33 0.23316636138091695 1107 34-35 0.4042864716527319 1107 36-37 0.2033588923694225 1107 38-39 0.1853223560931525 1107 40-41 0.1723358948771292 1107 42-43 0.1929898680840907 1107 44-45 0.27731908791626125 1107 46-47 0.3929853936302763 1107 48-49 0.37940429689891886 1107 50-51 0.27889605982622356 1107 52-53 0.23023081737564866 1107 54-55 0.3783282389935678 1107 56-57 0.44529297798582235 1107 58-59 0.36743504350948086 1107 60-61 0.4981170958799481 1107 62-63 0.5228800268882807 1107 64-65 0.644410457383664 1107 66-67 0.678344807478787 1107 68-69 0.5555489682246986 1107 70-71 0.6099852232224094 1107 72-73 0.6093916989872596 1107 74-75 0.6495391489393043 1107 76-77 0.5169143237236682 1107 78-79 0.616822596472236 1107 80-81 0.6508453347258687 1107 82-83 0.5673816890374894 1107 84-85 0.5346163244521982 1107 86-87 0.5703271404685779 1107 88-89 0.46935938505081154 1107 90-91 0.7244424544027268 1107 92-93 0.7827953252898432 1107 94-95 0.7770874759127331 1107 96-97 0.800350534509807 1107 98-99 0.9699001747886165 1107 100-101 1.2421524722009742 1108 1 0.11129610583714822 1108 2 0.023233962573936395 1108 3 0.07012512667661142 1108 4 0.195304493345148 1108 5 0.03030786790986184 1108 6 0.17809955442250214 1108 7 0.0757868161587254 1108 8 0.22321611133507702 1108 9 0.22278955728387473 1108 10-11 0.240171221378354 1108 12-13 0.18056999313193245 1108 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-1.9575022036363556 2316 84-85 -2.204817531808491 2316 86-87 -2.4491750389340723 2316 88-89 -2.3747090953787335 2316 90-91 -2.7011194447263733 2316 92-93 -2.783946052980337 2316 94-95 -2.8164602587150966 2316 96-97 -2.774293467662055 2316 98-99 -2.9743411065234646 2316 100-101 -3.568271412457669 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7471.0 3 1460.0 4 1220.0 5 1342.0 6 1536.0 7 1842.0 8 2131.0 9 2791.0 10 3524.0 11 4690.0 12 6038.0 13 8147.0 14 9389.0 15 11638.0 16 14975.0 17 19276.0 18 16503.0 19 11837.0 20 11219.0 21 11914.0 22 13024.0 23 14575.0 24 16739.0 25 19492.0 26 22255.0 27 26648.0 28 32347.0 29 38802.0 30 48437.0 31 60575.0 32 78160.0 33 103072.0 34 141259.0 35 200690.0 36 300902.0 37 441140.0 38 389130.0 39 53069.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.669461777742924 19.790628069114117 15.1898509647521 22.350059188390862 2 14.389279406858549 20.864935737565705 41.26443834235815 23.481346513217602 3 19.085380960623873 25.192079516510653 30.205393390327696 25.517146132537782 4 11.792888842020979 17.025167289662498 39.12853995937734 32.053403908939174 5 12.759854924977848 38.353894026923705 34.95154600644747 13.934705041650977 6 30.444610852432692 37.58559623847487 17.983766206516457 13.986026702575977 7 27.459208611157994 33.40380934087605 21.35180597294654 17.785176075019418 8 25.369997981172215 35.231668647418026 20.2509873839582 19.147345987451565 9 25.50072052659282 17.553995204570345 21.30987119101111 35.63541307782573 10-11 24.142739665790238 27.38954665942952 28.070034318158875 20.39767935662137 12-13 25.478485101609238 24.710829405260334 28.43291510303367 21.377770390096757 14-15 22.042499147956285 26.17615834818679 27.31440016821744 24.466942335639487 16-17 21.008148495929483 29.7917580693515 27.671223551890073 21.528869882828946 18-19 20.83544491465171 28.53432090845473 30.094078232397802 20.536155944495754 20-21 21.893941866156965 27.199086268385354 29.86874431831426 21.038227547143425 22-23 21.32938212253493 27.368902466740625 29.891027794217845 21.4106876165066 24-25 21.53271784334396 27.37713022179222 29.963847674035655 21.126304260828157 26-27 21.294064779222765 27.46942700753405 30.13934870226189 21.097159510981292 28-29 20.800602729533047 28.152289910667537 30.057359076772023 20.989748283027392 30-31 21.013605304196528 27.507254709003814 29.994197165353743 21.484942821445916 32-33 20.994395542170274 27.625284378270248 29.8792597294726 21.501060350086874 34-35 21.70403436894485 27.59461449992901 29.61029520278857 21.09105592833757 36-37 21.489848121577328 27.197561292842988 30.037467291292458 21.27512329428723 38-39 21.152711205920003 27.771714960464006 29.960086409520336 21.11548742409565 40-41 21.39733889489973 27.752157259070138 29.71859487161797 21.131908974412163 42-43 21.148551740631472 27.725140853983877 29.32974925182065 21.796558153564 44-45 21.363280028923825 27.7225820994436 29.16695513566901 21.747182735963563 46-47 21.61687744041028 28.02767061017542 28.94919284340579 21.40625910600851 48-49 21.63629166344173 28.03688641918437 28.866285331689628 21.460536585684274 50-51 21.15919708691169 28.254452734004442 29.024666084291468 21.561684094792405 52-53 21.63061893838317 28.495014533656853 28.385120221532755 21.48924630642722 54-55 21.22123295926496 28.095738874988026 28.677741303708753 22.005286862038265 56-57 21.587533547524735 28.301629975609217 28.470396021027582 21.640440455838466 58-59 22.041342783938262 27.929138642667706 28.452478592916147 21.577039980477885 60-61 21.66728389781822 28.00471348627148 29.18609157970078 21.14191103620952 62-63 21.3917476513396 28.191849648440975 29.001976077297765 21.414426622921656 64-65 21.18032030373268 28.4162992950894 28.73807134874604 21.665309052431876 66-67 21.329506509911404 28.32947038581698 28.365221586360473 21.975801517911144 68-69 21.265811810642113 28.199248681907285 28.70439229068119 21.830547216769418 70-71 21.51945231993004 28.209989399560133 28.5530119886593 21.717546291850528 72-73 21.506954741852493 27.88485787284982 28.906940850548686 21.701246534749004 74-75 21.19393619853034 28.64667900162368 28.406063428530672 21.753321371315305 76-77 21.35798656231972 28.117757434137303 28.468512356722048 22.055743646820932 78-79 21.41164607898621 28.207352081501668 28.49933850286276 21.881663336649364 80-81 21.514525360380677 28.43264561197411 28.319622027128922 21.733207000516288 82-83 21.45823306417498 28.20058570784296 28.687587911530848 21.65359331645121 84-85 21.319687405903416 28.370883405534254 28.37158257520036 21.937846613361973 86-87 21.380880011764628 28.294112862572714 28.4619359102868 21.86307121537586 88-89 21.397327906424984 28.22951776369363 28.159669314086827 22.21348501579456 90-91 21.30829568036158 27.94067262122019 28.58155393616312 22.169477762255106 92-93 21.635594697721857 28.003064313288977 28.66885559505198 21.69248539393719 94-95 21.331614297363856 27.859149986121395 28.618732691072037 22.190503025442712 96-97 21.04503394232508 27.910314327744327 28.882671981594143 22.161979748336442 98-99 21.12670738224968 28.199406444088247 28.164794391588877 22.509091782073195 100-101 21.013128748428382 27.621036293700836 29.00727458741798 22.3585603704528 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2861.0 1 1599.5 2 359.0 3 378.0 4 347.5 5 350.5 6 571.5 7 1134.0 8 1853.0 9 2237.5 10 2119.0 11 2045.5 12 2183.5 13 2408.0 14 2598.5 15 2700.0 16 2830.0 17 2992.5 18 3173.5 19 3447.0 20 3946.5 21 4627.5 22 5540.5 23 6797.0 24 8464.0 25 10702.0 26 13682.0 27 17175.0 28 20443.5 29 24216.0 30 29134.0 31 34332.5 32 40000.5 33 45779.5 34 52064.0 35 56975.0 36 61659.5 37 67722.5 38 71345.5 39 72355.0 40 73108.0 41 75662.0 42 78627.0 43 80693.5 44 82190.5 45 83347.5 46 82044.0 47 85732.5 48 99740.0 49 97887.0 50 81866.5 51 72590.0 52 66672.5 53 62593.0 54 57630.0 55 50673.5 56 43826.5 57 37461.0 58 32285.5 59 27845.5 60 23262.5 61 18552.0 62 13915.5 63 10011.0 64 7216.0 65 5307.0 66 3785.0 67 2826.5 68 2072.5 69 1402.0 70 1079.0 71 864.5 72 719.0 73 594.5 74 434.5 75 350.5 76 230.0 77 131.0 78 78.5 79 49.0 80 33.0 81 20.0 82 10.5 83 11.0 84 13.5 85 10.0 86 4.0 87 6.0 88 8.5 89 6.5 90 5.0 91 4.0 92 5.0 93 6.0 94 4.0 95 3.5 96 8.0 97 10.0 98 9.0 99 5.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32341318861395746 2 0.2837251653241134 3 0.27963073853688447 4 0.28493488232943104 5 0.28363211016985823 6 0.21407338236409412 7 0.20123177107687612 8 0.2072338285263367 9 0.2002546919571965 10-11 0.22200633426435018 12-13 0.21177026729627785 14-15 0.20565189090399824 16-17 0.2070477182178263 18-19 0.20220885019655577 20-21 0.19602068243858478 22-23 0.19850990781491146 24-25 0.18771550992130792 26-27 0.18932071133221015 28-29 0.20258107081357657 30-31 0.21502719769520995 32-33 0.2071640371606453 34-35 0.21646955258616557 36-37 0.19504360331890513 38-39 0.19211236595986625 40-41 0.18908807344657214 42-43 0.19646269442129696 44-45 0.20092934182554673 46-47 0.20416300843591498 48-49 0.2101650658853756 50-51 0.2139105358441475 52-53 0.22938095523907498 54-55 0.20995569178830137 56-57 0.18543565864205544 58-59 0.18627315503035227 60-61 0.18283011432290977 62-63 0.1909491765316762 64-65 0.1840398313282274 66-67 0.1805502630436573 68-69 0.17836346691866004 70-71 0.18932071133221015 72-73 0.1877853012869993 74-75 0.19262416930826987 76-77 0.1942758982962997 78-79 0.19211236595986625 80-81 0.19241479521119564 82-83 0.18590093441333144 84-85 0.17964297528966908 86-87 0.17906138057557405 88-89 0.18164366110615593 90-91 0.18676169459019207 92-93 0.18243462991732515 94-95 0.1802710975808917 96-97 0.17954992013541385 98-99 0.18873911661811515 100-101 0.17589750533089715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2149263.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.533876449191744 #Duplication Level Percentage of deduplicated Percentage of total 1 75.96686060374326 41.42767390397507 2 13.559821256555965 14.789392341562937 3 4.636298723719442 7.585060253425843 4 1.9914556510132009 4.344071857055945 5 1.0699973241292244 2.917555093751445 6 0.6480504614695577 2.120442227917353 7 0.40703799607990615 1.5538151851843742 8 0.278526082910417 1.2151285594651215 9 0.22161143851765908 1.0876797727064706 >10 1.0745434187904601 10.753630009056543 >50 0.08935960032866212 3.3992360719888897 >100 0.051758450700804357 4.8922910739230225 >500 0.0029775403899704746 1.1012395915385809 >1k 0.0014462339036999134 1.8015940118050726 >5k 2.552177477117494E-4 1.0111900466434054 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8348 0.3884122138612166 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7672 0.35695957172295806 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5776 0.26874328548902576 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4782 0.22249487382418995 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4378 0.20369773266463898 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3821 0.177781872204565 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3022 0.14060633807961148 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3016 0.14032717261684588 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2530 0.11771477013283159 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2304 0.10719953770199366 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2267 0.1054780173482724 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.3263788563800708E-5 0.0 22-23 0.0 0.0 0.0 9.305515425520283E-5 0.0 24-25 0.0 0.0 0.0 9.305515425520283E-5 0.0 26-27 0.0 0.0 0.0 2.791654627656085E-4 0.0 28-29 0.0 0.0 0.0 8.840239654244269E-4 0.0 30-31 0.0 0.0 0.0 0.002163532336433466 0.0 32-33 0.0 0.0 0.0 0.0034663044960063054 0.0 34-35 0.0 0.0 0.0 0.005513517889620768 0.0 36-37 0.0 0.0 0.0 0.009049613751318474 0.0 38-39 0.0 0.0 0.0 0.014307229966737434 0.0 40-41 0.0 0.0 0.0 0.019541582393592594 0.0 42-43 0.0 0.0 0.0 0.025566903631616976 0.0 44-45 0.0 2.3263788563800708E-5 0.0 0.030080078612994318 0.0 46-47 0.0 4.6527577127601416E-5 0.0 0.037501227164846745 0.0 48-49 0.0 4.6527577127601416E-5 0.0 0.043712658711381525 0.0 50-51 0.0 4.6527577127601416E-5 0.0 0.050528948760575135 0.0 52-53 0.0 4.6527577127601416E-5 0.0 0.06737193168076686 0.0 54-55 0.0 4.6527577127601416E-5 0.0 0.09468361945466888 0.0 56-57 0.0 4.6527577127601416E-5 0.0 0.13711676979504137 0.0 58-59 0.0 4.6527577127601416E-5 0.0 0.1876922461327441 0.0 60-61 0.0 4.6527577127601416E-5 0.0 0.25080690450633547 0.0 62-63 0.0 4.6527577127601416E-5 0.0 0.289494584887936 0.0 64-65 0.0 4.6527577127601416E-5 0.0 0.3412565144423926 0.0 66-67 0.0 4.6527577127601416E-5 0.0 0.4404998364555664 0.0 68-69 0.0 4.6527577127601416E-5 0.0 0.5703815680072657 0.0 70-71 0.0 4.6527577127601416E-5 0.0 0.702263985375452 0.0 72-73 0.0 4.6527577127601416E-5 0.0 0.86078344064919 0.0 74-75 0.0 4.6527577127601416E-5 0.0 1.0257702291436646 0.0 76-77 0.0 4.6527577127601416E-5 0.0 1.1771709651168796 0.0 78-79 0.0 4.6527577127601416E-5 0.0 1.3355741014477986 0.0 80-81 0.0 4.6527577127601416E-5 0.0 1.5059115613119474 0.0 82-83 0.0 9.305515425520283E-5 0.0 1.7185658525736498 0.0 84-85 0.0 9.305515425520283E-5 0.0 2.021576698617154 0.0 86-87 0.0 9.305515425520283E-5 0.0 2.387260191051537 0.0 88-89 0.0 9.305515425520283E-5 0.0 2.7999830639619256 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9235 0.0 26.052607 1 TACATGG 9575 0.0 24.823751 2 ACATGGG 9610 0.0 23.545042 3 GAGTACT 7045 0.0 22.587103 12-13 GTAGGAC 545 0.0 20.93296 3 GTACTTT 7695 0.0 20.61671 14-15 AGTACTT 7430 0.0 20.233995 12-13 GTGTAGC 1040 0.0 19.659508 1 GTATCAA 18175 0.0 19.411856 1 CATGGGA 5955 0.0 19.31924 4 AGAGTAC 12870 0.0 19.210512 10-11 GCGTATC 125 0.0059972955 19.019432 1 CATGGGG 3750 0.0 18.0017 4 GAGTGCG 135 0.009401953 17.592926 9 GTGTAAG 1055 0.0 17.5772 1 ATGGGAT 2855 0.0 17.483545 5 ATCAACG 20175 0.0 17.270555 3 CAACGCA 20205 0.0 17.246122 5 TCAACGC 20230 0.0 17.22521 4 TATCAAC 20580 0.0 16.97767 2 >>END_MODULE