FastQCFastQC Report
Thu 26 May 2016
SRR937234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2149263
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104200.48481735366960677No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT90900.4229356760898969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80100.37268589279208736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58870.2739078465501895No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40040.18629641881891607No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38070.17713048612477858No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29700.1381869040689762No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29280.13623274582961695No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23840.11092174387220177No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23050.10724606527912127No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22170.10315163849189235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG93000.023.0527571
TACATGG94900.022.3762632
ACATGGG93300.021.741653
GAGTACT92300.021.51348312-13
AGTACTT96200.021.45610212-13
GTACTTT98900.020.19506314-15
AGAGTAC153350.019.81038510-11
CAGTCCG3851.2732926E-1118.5083079
TACTTTT112500.018.38701214-15
GTGTAGC11400.018.347511
TATCGCG650.00756822918.2684790-91
CTTTGCG2352.526569E-618.1936952
GTATCAA225300.017.850011
CATGGGG37750.017.618034
CATGGGA56900.017.36594
TAGACTG11100.017.1191925
ACTTTTT118750.016.89929616-17
CTAGGAC13350.016.0131763
GTATAGG6850.015.9612391
TCAACGC251850.015.9012734
Produced by FastQC (version 0.11.5)