##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937234_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2149263 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20057433641206 33.0 31.0 34.0 31.0 34.0 2 32.41639529457307 34.0 31.0 34.0 31.0 34.0 3 32.50539510520583 34.0 31.0 34.0 31.0 34.0 4 36.1126209309889 37.0 35.0 37.0 35.0 37.0 5 35.97933012386106 37.0 35.0 37.0 35.0 37.0 6 36.07297152558807 37.0 35.0 37.0 35.0 37.0 7 36.041694292415585 37.0 35.0 37.0 35.0 37.0 8 36.01422254977636 37.0 35.0 37.0 35.0 37.0 9 37.72565572477635 39.0 37.0 39.0 35.0 39.0 10-11 37.643270274508055 39.0 37.0 39.0 35.0 39.0 12-13 37.56982649401213 39.0 37.0 39.0 35.0 39.0 14-15 38.789277999016406 40.0 38.0 41.0 35.0 41.0 16-17 38.780103691358384 40.0 38.0 41.0 35.0 41.0 18-19 38.7937472054374 40.0 38.0 41.0 35.0 41.0 20-21 38.715823284539866 40.0 38.0 41.0 34.0 41.0 22-23 38.59367583213408 40.0 38.0 41.0 34.0 41.0 24-25 38.44241095668608 40.0 38.0 41.0 34.0 41.0 26-27 38.24081417676664 40.0 38.0 41.0 34.0 41.0 28-29 38.25245560920185 40.0 38.0 41.0 34.0 41.0 30-31 38.38221683432879 40.0 38.0 41.0 34.0 41.0 32-33 38.347996033989325 40.0 38.0 41.0 34.0 41.0 34-35 38.23069652248236 40.0 38.0 41.0 34.0 41.0 36-37 38.079489573867875 40.0 38.0 41.0 33.5 41.0 38-39 37.95284802278734 40.0 37.0 41.0 33.0 41.0 40-41 37.817898507534906 40.0 37.0 41.0 33.0 41.0 42-43 37.62764282454032 40.0 37.0 41.0 33.0 41.0 44-45 37.35109104841985 40.0 37.0 41.0 32.5 41.0 46-47 37.033760875239565 40.0 37.0 41.0 31.5 41.0 48-49 36.73993503819682 40.0 36.5 41.0 31.0 41.0 50-51 36.548755317520474 39.5 36.0 41.0 31.0 41.0 52-53 36.352021599962406 39.0 36.0 41.0 30.0 41.0 54-55 36.171510420083536 39.0 36.0 40.5 30.0 41.0 56-57 35.94784444714304 39.0 35.0 40.0 29.5 41.0 58-59 35.70996011190813 39.0 35.0 40.0 29.0 41.0 60-61 35.38729973949209 38.0 35.0 40.0 28.0 41.0 62-63 35.061151892532465 38.0 34.0 40.0 28.0 41.0 64-65 34.74063202130219 37.5 34.0 40.0 27.0 41.0 66-67 34.366664991673886 37.0 34.0 39.5 26.0 41.0 68-69 33.937023528530474 36.5 33.5 39.0 26.0 40.5 70-71 33.55476668048536 36.0 33.0 39.0 26.0 40.0 72-73 33.117133640694504 35.5 33.0 38.5 25.5 40.0 74-75 32.68080267514958 35.0 32.0 37.5 24.5 39.5 76-77 31.34577224844051 34.0 30.5 36.0 23.0 39.0 78-79 31.98061777455807 35.0 32.0 36.0 24.0 39.0 80-81 31.787192167733778 35.0 32.0 36.0 24.0 38.5 82-83 31.44342502522958 35.0 31.5 36.0 22.5 37.0 84-85 31.09628370283209 34.0 31.0 35.0 21.5 37.0 86-87 30.775115702452425 34.0 31.0 35.0 20.0 36.5 88-89 30.40360276987972 34.0 31.0 35.0 18.5 36.0 90-91 29.96121624017163 34.0 30.0 35.0 16.5 36.0 92-93 29.569848361973385 34.0 30.0 35.0 9.0 35.0 94-95 29.175940078063967 34.0 29.5 35.0 2.0 35.0 96-97 28.761831613906722 34.0 29.0 35.0 2.0 35.0 98-99 28.136215297988194 34.0 29.0 35.0 2.0 35.0 100-101 26.489618766991292 32.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13302297694757925 1101 2 0.14968681866361777 1101 3 0.09509754700820139 1101 4 0.05952253847741673 1101 5 0.0015549168169854966 1101 6 0.08881349615392509 1101 7 0.027527264058761602 1101 8 0.01418196470169164 1101 9 0.14164362150322063 1101 10-11 0.11404946577251707 1101 12-13 0.19191200927092922 1101 14-15 0.17543169622951638 1101 16-17 0.12670646436701105 1101 18-19 0.09576730817707357 1101 20-21 0.017444898795375252 1101 22-23 -0.0017669264414053032 1101 24-25 0.006979719184066369 1101 26-27 0.047457721831897004 1101 28-29 0.045645277873624934 1101 30-31 0.16111144721566717 1101 32-33 0.1132487246905356 1101 34-35 0.1631575558474836 1101 36-37 0.044238477771280316 1101 38-39 0.007710384109998358 1101 40-41 0.07851071548854094 1101 42-43 0.03045105786657132 1101 44-45 0.1773039058810042 1101 46-47 0.15250328404228242 1101 48-49 0.11878227295917299 1101 50-51 0.15256717277506482 1101 52-53 0.28588157539287096 1101 54-55 0.1353837971905989 1101 56-57 0.18907331069153344 1101 58-59 0.09073560113078827 1101 60-61 0.05130511163028473 1101 62-63 -0.09841432338630085 1101 64-65 0.009526772633591918 1101 66-67 0.011499569909318552 1101 68-69 0.04488645710760153 1101 70-71 -0.05826192473433878 1101 72-73 -0.12581195769873688 1101 74-75 0.041864785046826114 1101 76-77 0.07330692418483054 1101 78-79 0.01815302096980176 1101 80-81 -0.14952305824354184 1101 82-83 -0.03182727478597158 1101 84-85 -0.02876297493619262 1101 86-87 -0.10431956702475986 1101 88-89 0.008833712190497778 1101 90-91 0.17314593654338495 1101 92-93 0.1986380107997192 1101 94-95 0.03365901122112547 1101 96-97 0.09558936891829006 1101 98-99 -0.30826277254954704 1101 100-101 -0.012330174101187197 1102 1 0.19592397203098955 1102 2 0.17563008905981548 1102 3 0.15500963246991262 1102 4 0.02821550996555544 1102 5 -0.004434359428778123 1102 6 0.01466873573181715 1102 7 0.10542701426191314 1102 8 0.13954773377776775 1102 9 0.2344335418035257 1102 10-11 0.15817200645000895 1102 12-13 0.13696131884119467 1102 14-15 0.19986059031072756 1102 16-17 0.21890788804491734 1102 18-19 0.23177047646572646 1102 20-21 0.23779887912900222 1102 22-23 0.2237000404557179 1102 24-25 0.32752330435713617 1102 26-27 0.33169218545265977 1102 28-29 0.17990929030053593 1102 30-31 0.17787697470289743 1102 32-33 0.14548223139510696 1102 34-35 0.207992397173129 1102 36-37 0.13481471711114068 1102 38-39 0.09331924341664433 1102 40-41 0.13495808372959317 1102 42-43 0.23003075687914531 1102 44-45 0.2509373761967595 1102 46-47 0.22840447132972486 1102 48-49 0.3955766960324141 1102 50-51 0.40007065775579065 1102 52-53 0.3214701027952813 1102 54-55 0.4070166696902362 1102 56-57 0.45528394306334263 1102 58-59 0.4635337191728013 1102 60-61 0.3429992808026725 1102 62-63 0.3407317229560789 1102 64-65 0.48192822189309936 1102 66-67 0.4551774591943172 1102 68-69 0.3213890686201495 1102 70-71 0.37517237841999673 1102 72-73 0.22972345025262797 1102 74-75 0.18741394482105278 1102 76-77 0.1250773559530849 1102 78-79 0.19692278515391237 1102 80-81 0.20079257362210612 1102 82-83 0.23607608693793125 1102 84-85 0.2328483549789624 1102 86-87 0.27896076769754785 1102 88-89 0.3441402434342571 1102 90-91 0.3099994025089039 1102 92-93 0.22821297995130863 1102 94-95 0.3010300498343028 1102 96-97 0.38499068411376314 1102 98-99 0.47289856393200935 1102 100-101 0.7009724487657572 1103 1 0.05701477751523498 1103 2 0.018734346142487368 1103 3 0.0036942595434865666 1103 4 0.010177877723158701 1103 5 -0.08120473828380881 1103 6 -0.005545355925548279 1103 7 0.04618558771328196 1103 8 -1.7088779313922942E-4 1103 9 -0.06897641249442188 1103 10-11 0.060828150325129116 1103 12-13 0.10847740089766233 1103 14-15 -0.006589356159160786 1103 16-17 -0.003971988663160175 1103 18-19 -0.03834648394501272 1103 20-21 -0.031013284868834035 1103 22-23 0.012670535580454612 1103 24-25 0.06981863802814559 1103 26-27 0.03942462412327785 1103 28-29 0.023118952773053536 1103 30-31 -0.044311831172755944 1103 32-33 0.024199120432371046 1103 34-35 0.14879989599316445 1103 36-37 0.13478701671857607 1103 38-39 0.09771569143487824 1103 40-41 0.15230464513957287 1103 42-43 0.1113050756653351 1103 44-45 0.13295313016547539 1103 46-47 0.03268177168544639 1103 48-49 0.011358940728321443 1103 50-51 0.04085952176629348 1103 52-53 0.20960512530096054 1103 54-55 0.10845104638103464 1103 56-57 0.10814622102851246 1103 58-59 -0.06047604575519472 1103 60-61 -0.08557579661452763 1103 62-63 0.17124489251414587 1103 64-65 0.20502862250552312 1103 66-67 0.3315495279891394 1103 68-69 0.21316615312864684 1103 70-71 0.19791360926715384 1103 72-73 0.14328383566940772 1103 74-75 0.1262993241941217 1103 76-77 -0.04613769118795119 1103 78-79 0.3668096524337763 1103 80-81 0.26521361383300857 1103 82-83 0.1793802185414144 1103 84-85 0.11813743195871496 1103 86-87 0.19079365590595998 1103 88-89 0.11035360696701346 1103 90-91 0.3405881905411867 1103 92-93 0.48565179446084983 1103 94-95 0.42619337422676296 1103 96-97 0.347878633507662 1103 98-99 0.4030158696982937 1103 100-101 0.6959169472519484 1104 1 0.04085998086772946 1104 2 0.10466022389524454 1104 3 0.1972937165114459 1104 4 0.08956989320120101 1104 5 -0.014541052331921378 1104 6 -0.03114021175379378 1104 7 0.046098198575542426 1104 8 0.05841641598776448 1104 9 0.07153312714773108 1104 10-11 0.21894761737332402 1104 12-13 0.11244649359517922 1104 14-15 0.18678349874235067 1104 16-17 0.15745708793850355 1104 18-19 0.12302670425461315 1104 20-21 0.031171199697837437 1104 22-23 -6.695179098272774E-4 1104 24-25 0.03230126181587423 1104 26-27 0.06325035798879952 1104 28-29 -0.009929383581642526 1104 30-31 0.0020277833395354605 1104 32-33 0.023672753300438387 1104 34-35 0.057627621518108185 1104 36-37 0.14818702322195776 1104 38-39 0.1570468190392802 1104 40-41 0.08562165228515539 1104 42-43 0.20252104470925758 1104 44-45 0.24498194012865326 1104 46-47 0.2599575474164624 1104 48-49 0.2342053065595806 1104 50-51 0.3504040414512062 1104 52-53 0.468834840859472 1104 54-55 0.5285651806595268 1104 56-57 0.44144636003820636 1104 58-59 0.3680061065376492 1104 60-61 0.34019017933923834 1104 62-63 0.4917953627896381 1104 64-65 0.5507481806010119 1104 66-67 0.5015813538937266 1104 68-69 0.4775619893410834 1104 70-71 0.536047586976828 1104 72-73 0.5251957067724646 1104 74-75 0.4218270326476983 1104 76-77 0.4137151292456629 1104 78-79 0.4003670813234059 1104 80-81 0.48736899578450377 1104 82-83 0.4947259543579534 1104 84-85 0.520250420016886 1104 86-87 0.6172130180871918 1104 88-89 0.5381092100333547 1104 90-91 0.7011395143850052 1104 92-93 0.6961427588304687 1104 94-95 0.5426160287429518 1104 96-97 0.44497101398777517 1104 98-99 0.7114944451334111 1104 100-101 0.9981898777552267 1105 1 0.06600227180727813 1105 2 0.06933814275109285 1105 3 -0.059783432407044756 1105 4 0.010262489831326604 1105 5 0.017573470548846615 1105 6 0.007985290541661527 1105 7 0.05201538115228743 1105 8 0.05478822594778876 1105 9 0.02176575656862667 1105 10-11 0.10024415410101284 1105 12-13 0.03782820922150876 1105 14-15 0.032718532145182166 1105 16-17 0.045739543525897375 1105 18-19 0.05470112523530446 1105 20-21 0.03235984109768708 1105 22-23 0.09797297110448966 1105 24-25 0.008437733887340926 1105 26-27 0.07485493713051738 1105 28-29 -0.08061923318939535 1105 30-31 -0.11172034073334913 1105 32-33 -0.01324260081965889 1105 34-35 0.020520361378949303 1105 36-37 0.03748529876661877 1105 38-39 -0.027863642489393214 1105 40-41 -0.11002234132633504 1105 42-43 -0.23678971291951 1105 44-45 -0.07364316071598864 1105 46-47 -0.13780078594096778 1105 48-49 -0.15233997063425875 1105 50-51 -0.09113459951490199 1105 52-53 -0.1366954453031468 1105 54-55 -0.1256455227035005 1105 56-57 -0.09865900995679056 1105 58-59 -0.02137959178917015 1105 60-61 -0.07356951114363852 1105 62-63 -0.02758483185376548 1105 64-65 0.010591532855066532 1105 66-67 -0.14697578465354866 1105 68-69 -0.03302927589886906 1105 70-71 -0.06143276699386746 1105 72-73 -0.10265910008294554 1105 74-75 -0.1668785607717922 1105 76-77 -0.06946962391269196 1105 78-79 -0.08537587256420665 1105 80-81 -0.12289310870451331 1105 82-83 -0.20268230232381867 1105 84-85 -0.30002356895839455 1105 86-87 -0.25098889673198244 1105 88-89 -0.10265785499975166 1105 90-91 -0.18467341628304368 1105 92-93 0.05900157055533839 1105 94-95 0.10284772321237767 1105 96-97 0.019917977178643298 1105 98-99 -0.021452666493047445 1105 100-101 0.15806137494025663 1106 1 -0.13403263088569872 1106 2 0.07382355931262907 1106 3 0.004217383390837881 1106 4 0.018416473784498066 1106 5 -0.02651451787859571 1106 6 0.012322265529462584 1106 7 -0.012656575585189955 1106 8 0.054175957020440535 1106 9 0.11433779068075722 1106 10-11 0.002324123319361604 1106 12-13 -0.08501192382924927 1106 14-15 0.010219120865308184 1106 16-17 0.13096414025260827 1106 18-19 0.16665277449267535 1106 20-21 -0.059706103966320256 1106 22-23 -0.0017818690159430162 1106 24-25 0.04117982401197651 1106 26-27 0.19869084821696248 1106 28-29 0.09869745197990198 1106 30-31 0.1222366154154173 1106 32-33 0.09354607809238757 1106 34-35 0.07414109526062873 1106 36-37 0.14816657410260348 1106 38-39 0.19515415788401924 1106 40-41 0.19633531035319862 1106 42-43 0.01051910022072633 1106 44-45 0.08012286088163023 1106 46-47 0.19734527972656224 1106 48-49 0.025122982049460063 1106 50-51 0.16299201315771938 1106 52-53 0.25410370021164397 1106 54-55 0.15282992346224944 1106 56-57 0.20032765553244047 1106 58-59 0.08598855592912003 1106 60-61 0.20818605226152442 1106 62-63 0.24668999110968315 1106 64-65 0.06446468048925169 1106 66-67 0.18586638789190602 1106 68-69 0.16056439788289367 1106 70-71 0.21615644513040166 1106 72-73 0.1916075018245067 1106 74-75 0.21119087876665787 1106 76-77 0.14242830463350842 1106 78-79 0.2349062715544754 1106 80-81 0.25735385938685695 1106 82-83 0.11782691955638924 1106 84-85 0.10778054703629181 1106 86-87 0.179760901421119 1106 88-89 0.25305840713209093 1106 90-91 0.2391606024887203 1106 92-93 0.18204207870490663 1106 94-95 -0.016336352749341643 1106 96-97 0.05078741167091039 1106 98-99 -0.12371986973605686 1106 100-101 0.12999487900242102 1107 1 -0.011787520773530957 1107 2 0.03641917117851534 1107 3 0.0775433831438832 1107 4 -0.019066339440307445 1107 5 -0.07638745254548951 1107 6 -0.03028195641020659 1107 7 -0.04714546925745111 1107 8 -0.021092106614013062 1107 9 0.04471800219489097 1107 10-11 0.001498261227375508 1107 12-13 0.016509877511659 1107 14-15 0.07052613840915711 1107 16-17 0.08023791556973237 1107 18-19 0.0674206312183756 1107 20-21 0.045171394373404894 1107 22-23 0.10275168524253786 1107 24-25 0.16277959574490097 1107 26-27 0.12602280577396385 1107 28-29 0.01405185413042176 1107 30-31 0.07620567588282512 1107 32-33 -0.026340126266717334 1107 34-35 0.05578221954270646 1107 36-37 0.12217804252065889 1107 38-39 0.1802680953575333 1107 40-41 0.0954742821168324 1107 42-43 0.19958207473423784 1107 44-45 0.10767332409025698 1107 46-47 0.08778240366156354 1107 48-49 0.07526915306956283 1107 50-51 0.08446899539360686 1107 52-53 0.08831639573207184 1107 54-55 0.15441834441476487 1107 56-57 0.16413541477741944 1107 58-59 0.12493568657197329 1107 60-61 0.1489609255299129 1107 62-63 0.10593051085111682 1107 64-65 0.11390940581789266 1107 66-67 0.18343854731843123 1107 68-69 0.0104810920817755 1107 70-71 -0.11718512700900163 1107 72-73 -0.05362619243110345 1107 74-75 0.11363601040400795 1107 76-77 0.08113315180469627 1107 78-79 0.07267886131783641 1107 80-81 -0.026894336001749508 1107 82-83 0.08243791024883151 1107 84-85 0.10569081037773032 1107 86-87 0.156569705221564 1107 88-89 0.19233882355263532 1107 90-91 0.37893424181339697 1107 92-93 0.2976161545403002 1107 94-95 0.2338494896863068 1107 96-97 0.4240502750544053 1107 98-99 0.4999300089553458 1107 100-101 0.7759501258078494 1108 1 -0.002575847679850085 1108 2 0.005594874515715276 1108 3 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78-79 -0.38798591035660834 2316 80-81 -0.4313118278895871 2316 82-83 -0.5620949725525257 2316 84-85 -0.473372730575214 2316 86-87 -0.44545809266604053 2316 88-89 -0.42401884303215454 2316 90-91 -0.650569031061174 2316 92-93 -0.6687294192146496 2316 94-95 -0.6857100296600649 2316 96-97 -0.6339523503555142 2316 98-99 -1.154520475619698 2316 100-101 -1.4118101797609874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 11.0 5 27.0 6 53.0 7 151.0 8 340.0 9 793.0 10 1463.0 11 2399.0 12 3800.0 13 5417.0 14 7196.0 15 10154.0 16 13943.0 17 19878.0 18 17106.0 19 9776.0 20 8037.0 21 8083.0 22 8496.0 23 9111.0 24 10989.0 25 12852.0 26 16415.0 27 20476.0 28 25875.0 29 33246.0 30 42430.0 31 54799.0 32 70848.0 33 94031.0 34 132557.0 35 192308.0 36 306227.0 37 500083.0 38 462449.0 39 47431.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.3838362209416 20.321702214815712 15.593224283839014 21.701237280403678 2 15.152356877683188 21.474244892318904 40.290555413646445 23.082842816351466 3 19.468068821730984 25.31774845609867 30.64985532249892 24.564327399671424 4 12.476189501326502 17.796341255742668 38.677648420713716 31.049820822217118 5 12.846248915790923 38.49602984041187 34.82223624403918 13.83548499975803 6 30.560661957145307 37.91127470207229 17.608408091517884 13.919655249264515 7 27.62342254065696 33.6510236299606 21.109515215215634 17.61603861416681 8 25.68517673267534 35.28288534255696 19.958562539810156 19.07337538495754 9 25.699414171276384 17.483574602084527 21.10374579565181 35.71326543098727 10-11 24.309193430492222 27.515711199606564 27.84554519386413 20.32955017603709 12-13 25.76392220326373 24.749353113037362 28.399372958193958 21.08735172550496 14-15 22.18707063770232 26.221709488322276 27.44878127990851 24.142438594066896 16-17 21.03011590484738 29.745964081641006 27.88081309732685 21.343106916184755 18-19 20.870223886048382 28.230281729132265 30.56280222569318 20.336692159126173 20-21 21.8187118095831 26.847319290380007 30.58115735487002 20.752811545166878 22-23 21.30545266994272 26.9970917511678 30.581884734147994 21.11557084474148 24-25 21.64516344411066 27.021360692315444 30.617868592856627 20.71560727071727 26-27 21.440344832961067 27.14321906910641 30.78029205553936 20.636144042393163 28-29 20.805431426449704 27.832245174474547 30.74873115341737 20.613592245658378 30-31 21.12220572802346 27.12213919289163 30.641870930684583 21.11378414840033 32-33 21.059730707689102 27.248782489625512 30.54579639625304 21.145690406432344 34-35 21.770509239678905 27.108385525642976 30.303713412458134 20.81739182221999 36-37 21.55251290271175 26.83126614656457 30.642442797105513 20.97377815361817 38-39 21.188146037512514 27.392106849998328 30.717792575663395 20.701954536825767 40-41 21.35132896780383 27.401056734823392 30.428002728378395 20.819611568994382 42-43 21.21700289921027 27.372490936041714 29.959607423523178 21.450898741224833 44-45 21.45060888313808 27.28060735238079 29.782790658937508 21.48599310554362 46-47 21.784095292200163 27.759562231332325 29.429018226247788 21.027324250219724 48-49 21.830237542002788 27.80842912590461 29.24150243199759 21.11983090009501 50-51 21.343269762704704 28.003808747463665 29.378442749910082 21.274478739921545 52-53 21.818493553818833 28.372874905141636 28.599348519046348 21.20928302199318 54-55 21.34145518719673 27.884209610457166 29.025786979071434 21.74854822327468 56-57 21.782583145943516 28.012835097426418 28.78533246047599 21.419249296154078 58-59 22.296154542277982 27.665367151437493 28.754414885474695 21.284063420809833 60-61 21.797029958641637 27.734483867260547 29.606497669201026 20.861988504896793 62-63 21.539755720914563 27.91682544202361 29.378559068852905 21.164859768208917 64-65 21.32451914912228 28.219557122604357 28.999056886011626 21.456866842261743 66-67 21.505162467320194 28.098678477226848 28.669641639948207 21.726517415504755 68-69 21.45035298146388 27.903425499810865 29.01648146364591 21.629740055079345 70-71 21.73465974150209 27.94092672697571 28.874200132789706 21.450213398732494 72-73 21.730984062909005 27.612814253071864 29.23583572601399 21.42036595800514 74-75 21.455005739176638 28.365467604476514 28.693696397323176 21.485830259023675 76-77 21.538825169372014 27.85124482206226 28.827905193547743 21.782024815017987 78-79 21.576349660325423 27.914406008012982 28.797406366740596 21.711837964921 80-81 21.639161889447685 28.1685628980725 28.645261189533343 21.54701402294647 82-83 21.642592433191968 27.986697716185375 28.906318701131323 21.464391149491334 84-85 21.414872367758033 28.1280868168175 28.701120989958874 21.75591982546559 86-87 21.413130919761798 28.08586012972819 28.712237636808524 21.78877131370149 88-89 21.38016613136689 27.94739406019645 28.4835313314378 22.188908476998858 90-91 21.31947489952248 27.690616475405545 28.838927696898136 22.150980928173837 92-93 21.65132889248607 27.67178427281223 28.905746727467175 21.771140107234526 94-95 21.36730125629111 27.596506337288645 28.914353431850827 22.12183897456942 96-97 21.060963688482982 27.593831001603807 29.231857618169578 22.113347691743634 98-99 21.11526943147538 27.891227520817257 28.538480024436563 22.4550230232708 100-101 20.81277455809539 27.641417268277678 29.06797349398319 22.477834679643735 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 343.0 1 238.5 2 211.5 3 325.5 4 287.0 5 251.5 6 512.0 7 1275.0 8 2236.5 9 2718.5 10 2528.0 11 2253.5 12 2393.0 13 2642.5 14 2844.5 15 3042.0 16 3255.5 17 3428.5 18 3558.0 19 3849.5 20 4271.5 21 4942.0 22 5851.0 23 7127.5 24 8785.0 25 10867.0 26 13782.0 27 17167.0 28 20305.5 29 23971.5 30 29019.0 31 34338.5 32 39957.5 33 45485.5 34 51508.0 35 56626.0 36 61646.0 37 67642.5 38 71185.0 39 72346.0 40 73024.5 41 75272.5 42 78783.0 43 81307.5 44 82492.0 45 83393.5 46 82128.5 47 86177.5 48 101048.0 49 98702.5 50 81315.0 51 72466.5 52 66838.5 53 62904.5 54 57725.0 55 50168.5 56 42983.0 57 36643.0 58 31977.5 59 27846.5 60 23344.0 61 18353.0 62 13716.5 63 9988.5 64 7023.5 65 5084.0 66 3723.5 67 2801.0 68 1934.5 69 1213.5 70 920.5 71 728.0 72 607.0 73 532.5 74 407.5 75 328.5 76 219.5 77 117.5 78 73.5 79 45.0 80 27.5 81 14.5 82 8.5 83 3.0 84 1.5 85 0.5 86 0.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04666715985898422 2 0.0 3 0.0 4 4.6527577127601416E-5 5 0.010747870316475927 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005769419563822575 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014656186795194445 24-25 5.350671369674163E-4 26-27 0.0022565874906886685 28-29 0.003117347667549295 30-31 0.0010468704853710317 32-33 0.0 34-35 0.0 36-37 2.559016742018078E-4 38-39 3.2569303989320994E-4 40-41 4.6527577127601416E-5 42-43 0.010678078950784525 44-45 0.0 46-47 0.0 48-49 4.6527577127601416E-5 50-51 0.0 52-53 2.791654627656085E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.722206170208113E-4 84-85 9.305515425520283E-5 86-87 0.0 88-89 0.0 90-91 0.007002400357704013 92-93 0.0022565874906886685 94-95 0.0 96-97 0.0 98-99 0.0011399256396262348 100-101 0.011236409876315741 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2149263.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.660537157923414 #Duplication Level Percentage of deduplicated Percentage of total 1 74.4411049754285 39.2010857463543 2 14.299564069629872 15.06045450061701 3 4.855675575925435 7.671074522785277 4 2.2799715089805437 4.802580974707067 5 1.137037933726342 2.9938514179482256 6 0.702488761960266 2.219606133133932 7 0.45086142110380456 1.661982323337774 8 0.29845926734455674 1.2573620270499704 9 0.22167923718296773 1.050637293613241 >10 1.1584233137804718 11.099432381760458 >50 0.09188397306165651 3.368151152539192 >100 0.05818061615327913 5.4557309380499985 >500 0.0029954293313285566 1.0794118203028185 >1k 0.0013215129402919872 1.5287414635432695 >5k 2.6430258805839745E-4 1.0664677861815766 >10k+ 8.810086268613248E-5 0.4834295180759572 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10420 0.48481735366960677 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9090 0.4229356760898969 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8010 0.37268589279208736 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5887 0.2739078465501895 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4004 0.18629641881891607 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3807 0.17713048612477858 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2970 0.1381869040689762 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2928 0.13623274582961695 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2384 0.11092174387220177 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2305 0.10724606527912127 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2217 0.10315163849189235 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.6527577127601416E-5 0.0 9 0.0 0.0 0.0 4.6527577127601416E-5 0.0 10-11 0.0 0.0 0.0 4.6527577127601416E-5 0.0 12-13 0.0 0.0 0.0 4.6527577127601416E-5 0.0 14-15 0.0 0.0 0.0 4.6527577127601416E-5 0.0 16-17 0.0 0.0 0.0 4.6527577127601416E-5 0.0 18-19 0.0 0.0 0.0 4.6527577127601416E-5 0.0 20-21 0.0 0.0 0.0 9.305515425520283E-5 0.0 22-23 0.0 0.0 0.0 1.8611030851040566E-4 0.0 24-25 0.0 0.0 0.0 1.8611030851040566E-4 0.0 26-27 0.0 0.0 0.0 3.722206170208113E-4 0.0 28-29 0.0 0.0 0.0 9.305515425520283E-4 0.0 30-31 0.0 0.0 0.0 0.0022100599135610674 0.0 32-33 0.0 0.0 0.0 0.0035128320731339065 0.0 34-35 0.0 0.0 0.0 0.005629836832439771 0.0 36-37 0.0 0.0 0.0 0.009258987848392682 0.0 38-39 0.0 0.0 0.0 0.014586395429503043 0.0 40-41 0.0 0.0 0.0 0.020146440896251415 0.0 42-43 0.0 0.0 0.0 0.0263113448656586 0.0 44-45 0.0 0.0 0.0 0.03089431121272734 0.0 46-47 0.0 0.0 0.0 0.03840851491883497 0.0 48-49 0.0 0.0 0.0 0.04468973783106116 0.0 50-51 0.0 0.0 0.0 0.05155255545738237 0.0 52-53 0.0 0.0 0.0 0.06869796762890348 0.0 54-55 0.0 0.0 0.0 0.09640513980839013 0.0 56-57 0.0 0.0 0.0 0.13869870741737983 0.0 58-59 0.0 0.0 0.0 0.18941376648646535 0.0 60-61 0.0 0.0 0.0 0.2533426574597897 0.0 62-63 0.0 0.0 0.0 0.2925654049783577 0.0 64-65 0.0 0.0 0.0 0.3448391378812179 0.0 66-67 0.0 0.0 0.0 0.44447794429997634 0.0 68-69 0.0 0.0 0.0 0.5748482154115155 0.0 70-71 0.0 2.3263788563800708E-5 0.0 0.7073354912823605 0.0 72-73 0.0 4.6527577127601416E-5 0.0 0.8667389705215229 0.0 74-75 0.0 4.6527577127601416E-5 0.0 1.0332611690612086 0.0 76-77 0.0 4.6527577127601416E-5 0.0 1.1857785668854859 0.0 78-79 0.0 4.6527577127601416E-5 0.0 1.345461211587414 0.0 80-81 0.0 4.6527577127601416E-5 0.0 1.51682227814837 0.0 82-83 0.0 4.6527577127601416E-5 0.0 1.7313144087066124 0.0 84-85 0.0 4.6527577127601416E-5 0.0 2.0387686383658026 0.0 86-87 0.0 4.6527577127601416E-5 0.0 2.408918778204436 0.0 88-89 0.0 4.6527577127601416E-5 0.0 2.8272016965815725 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9300 0.0 23.052757 1 TACATGG 9490 0.0 22.376263 2 ACATGGG 9330 0.0 21.74165 3 GAGTACT 9230 0.0 21.513483 12-13 AGTACTT 9620 0.0 21.456102 12-13 GTACTTT 9890 0.0 20.195063 14-15 AGAGTAC 15335 0.0 19.810385 10-11 CAGTCCG 385 1.2732926E-11 18.508307 9 TACTTTT 11250 0.0 18.387012 14-15 GTGTAGC 1140 0.0 18.34751 1 TATCGCG 65 0.007568229 18.26847 90-91 CTTTGCG 235 2.526569E-6 18.193695 2 GTATCAA 22530 0.0 17.85001 1 CATGGGG 3775 0.0 17.61803 4 CATGGGA 5690 0.0 17.3659 4 TAGACTG 1110 0.0 17.119192 5 ACTTTTT 11875 0.0 16.899296 16-17 CTAGGAC 1335 0.0 16.013176 3 GTATAGG 685 0.0 15.961239 1 TCAACGC 25185 0.0 15.901273 4 >>END_MODULE