FastQCFastQC Report
Thu 26 May 2016
SRR937233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346312
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87180.37156183832329204No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT83450.3556645493012012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60930.25968413407935514No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51190.2181721782951287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45340.19323943277790848No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39760.16945742936148306No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34580.14738022905734618No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32050.136597349372121No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26190.11162198377709359No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24650.10505849179478263No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23820.10152102533678385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG97850.024.63721
TACATGG100500.023.6482832
ACATGGG101100.022.5676213
GAGTACT80600.021.92310512-13
AGTACTT86050.020.83820212-13
GTACTTT87300.020.21250714-15
AGAGTAC138000.020.18774210-11
GTATCAA193250.020.0285321
CTACCGT1200.004745227319.805864
CATGGGA63450.018.654034
TACTTTT100500.017.72314514-15
CATGGGG40800.017.708774
CAACGCA216500.017.3453645
GTATAGG7950.017.3450321
TATCAAC219000.017.3202632
ATCAACG217350.017.2986623
TCAACGC217150.017.2930744
AACGCAG218850.017.1481176
GAGTACA84300.016.7522491
ACTTTTT108200.016.59358216-17
Produced by FastQC (version 0.11.5)