##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937233_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2346312 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.661931576022287 33.0 31.0 34.0 30.0 34.0 2 31.761550041085755 33.0 31.0 34.0 30.0 34.0 3 31.820895089826077 33.0 31.0 34.0 30.0 34.0 4 35.448988881274104 37.0 35.0 37.0 33.0 37.0 5 35.43474525127093 37.0 35.0 37.0 33.0 37.0 6 35.505941238846326 37.0 35.0 37.0 33.0 37.0 7 35.44433093297055 37.0 35.0 37.0 33.0 37.0 8 35.43464424168653 37.0 35.0 37.0 33.0 37.0 9 37.04709390737464 39.0 37.0 39.0 33.0 39.0 10-11 36.95613989102898 39.0 37.0 39.0 33.0 39.0 12-13 36.85558335805298 39.0 37.0 39.0 33.0 39.0 14-15 38.00951237516579 40.0 38.0 41.0 33.0 41.0 16-17 37.961100015684195 40.0 38.0 41.0 33.0 41.0 18-19 37.92065228324281 40.0 38.0 41.0 33.0 41.0 20-21 37.851835561511 40.0 38.0 41.0 33.0 41.0 22-23 37.72045810616832 40.0 37.5 41.0 32.0 41.0 24-25 37.57269195230643 40.0 37.0 41.0 32.0 41.0 26-27 37.415568773462354 40.0 37.0 41.0 32.0 41.0 28-29 37.24628097201054 39.0 37.0 41.0 31.5 41.0 30-31 37.268583845626665 39.0 37.0 41.0 32.0 41.0 32-33 37.14098870908899 39.0 36.0 41.0 31.0 41.0 34-35 36.948977160752705 39.0 36.0 41.0 31.0 41.0 36-37 36.9510316616034 39.0 36.0 41.0 31.0 41.0 38-39 36.938015916041856 39.0 36.0 41.0 31.0 41.0 40-41 36.80903924968206 39.5 36.0 41.0 30.5 41.0 42-43 36.59271273385637 39.0 36.0 41.0 30.0 41.0 44-45 36.38063181708145 39.0 36.0 41.0 30.0 41.0 46-47 36.094256219974156 39.0 35.0 41.0 29.5 41.0 48-49 35.823005422978696 39.0 35.0 41.0 28.0 41.0 50-51 34.91639070166286 38.0 34.0 39.5 26.5 40.5 52-53 35.08045349467589 38.5 34.5 40.0 27.0 40.5 54-55 35.456034406336414 39.0 35.0 40.0 27.0 41.0 56-57 35.255302790080776 39.0 35.0 40.0 26.5 41.0 58-59 35.03016393386728 38.0 34.5 40.0 26.0 41.0 60-61 34.75780522794923 38.0 34.0 40.0 26.0 41.0 62-63 34.46417228399292 38.0 34.0 40.0 25.5 41.0 64-65 34.12959529678918 37.5 34.0 40.0 24.5 41.0 66-67 33.745838149402125 37.0 33.0 39.5 23.0 41.0 68-69 33.26019195230643 36.0 33.0 39.0 22.0 41.0 70-71 32.833690915786136 36.0 32.0 39.0 21.5 40.0 72-73 32.41829006543034 35.0 32.0 38.5 20.5 40.0 74-75 31.960563215804207 35.0 31.0 37.5 19.0 39.5 76-77 31.521942094657486 35.0 31.0 37.0 18.0 39.0 78-79 31.036427593602212 35.0 31.0 36.5 14.5 39.0 80-81 30.563432953503202 34.0 30.0 36.0 9.5 38.5 82-83 30.101759484672115 34.0 30.0 35.5 7.0 37.0 84-85 29.620020909410172 34.0 29.0 35.0 4.0 37.0 86-87 29.195733772831574 34.0 29.0 35.0 2.0 36.0 88-89 28.78914334496009 34.0 29.0 35.0 2.0 36.0 90-91 28.34413773615785 34.0 28.5 35.0 2.0 35.5 92-93 27.900993346153452 34.0 27.5 35.0 2.0 35.0 94-95 27.353186831077878 33.0 26.5 35.0 2.0 35.0 96-97 26.72268607073569 33.0 25.0 35.0 2.0 35.0 98-99 25.999065554794075 32.5 24.5 35.0 2.0 35.0 100-101 24.116509654300025 30.5 11.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.020763131140942193 1101 2 0.06548254167865863 1101 3 0.1653036455755057 1101 4 0.10957837581754148 1101 5 0.15934262934392507 1101 6 0.014876614106164254 1101 7 0.15593886876988705 1101 8 -0.002852736533313305 1101 9 0.02126521863704056 1101 10-11 0.07324340779887706 1101 12-13 0.1851900574463059 1101 14-15 0.1556095679713252 1101 16-17 0.02753086558924167 1101 18-19 0.23391907604860052 1101 20-21 0.21983968437686485 1101 22-23 0.13028979021258635 1101 24-25 0.18387725793904508 1101 26-27 0.18181833032790706 1101 28-29 0.2576523117881351 1101 30-31 0.11856074614535572 1101 32-33 0.11512063684794072 1101 34-35 -0.035150222300003975 1101 36-37 0.08923505038774948 1101 38-39 0.27943367690711796 1101 40-41 0.11743981916344381 1101 42-43 0.1891763942432121 1101 44-45 -0.03791961812310518 1101 46-47 0.05717623419503326 1101 48-49 0.0640178043110069 1101 50-51 0.14402553704130838 1101 52-53 0.10915290094882835 1101 54-55 0.18958047140225176 1101 56-57 0.26584327301532085 1101 58-59 0.29731697284434944 1101 60-61 0.11906610939858098 1101 62-63 0.168077043479947 1101 64-65 0.10243187388037711 1101 66-67 0.1590434394442113 1101 68-69 0.09031093324882278 1101 70-71 0.11666832366299218 1101 72-73 0.08823786398844646 1101 74-75 0.13993762089594952 1101 76-77 0.1324131326799396 1101 78-79 0.17665222725223018 1101 80-81 0.2315610153047416 1101 82-83 0.13874609577020536 1101 84-85 0.2052489035958338 1101 86-87 0.2609236880819559 1101 88-89 0.2279476247909109 1101 90-91 0.37669310092231356 1101 92-93 0.21173085922729484 1101 94-95 0.1995023998901324 1101 96-97 0.31542606774448956 1101 98-99 0.46704797260777653 1101 100-101 1.0284327708172505 1102 1 -0.06790865586440731 1102 2 -0.18101540172348507 1102 3 -0.16967270146331614 1102 4 -0.21978597990296578 1102 5 -0.124414240384219 1102 6 -0.09157124628116975 1102 7 -0.056717581057107225 1102 8 -0.05341041960389248 1102 9 0.07524450605657051 1102 10-11 -0.08828027436349117 1102 12-13 0.07066419532596768 1102 14-15 0.05703440709662999 1102 16-17 0.08705361092986408 1102 18-19 0.21130126713877928 1102 20-21 0.28496399204055933 1102 22-23 0.30261254794881864 1102 24-25 0.28056983618928655 1102 26-27 0.2922135654093765 1102 28-29 0.28448566955512433 1102 30-31 0.1748685302282098 1102 32-33 0.09087948033792514 1102 34-35 0.030919995230959785 1102 36-37 0.17647761808981244 1102 38-39 0.25956703693867667 1102 40-41 0.10482967407526189 1102 42-43 0.14517497351609165 1102 44-45 0.10055881531069844 1102 46-47 0.16385457537693782 1102 48-49 0.18215165182360238 1102 50-51 0.07019629604570099 1102 52-53 0.0635199384109626 1102 54-55 0.18207785223459183 1102 56-57 0.1534176615484384 1102 58-59 0.05564199277863224 1102 60-61 0.11567430217885999 1102 62-63 0.2711380131738892 1102 64-65 0.23809477657368205 1102 66-67 0.2628448677994015 1102 68-69 0.29738891352788954 1102 70-71 0.14141081687223078 1102 72-73 0.12025160165691062 1102 74-75 0.2041361317546233 1102 76-77 0.2210111717904617 1102 78-79 0.36317614779163065 1102 80-81 0.2641014172322613 1102 82-83 0.35702258214040583 1102 84-85 0.21164917796779648 1102 86-87 0.30228091156161696 1102 88-89 0.4854073360924893 1102 90-91 0.5365789838768045 1102 92-93 0.24405657283874405 1102 94-95 0.12009409340401689 1102 96-97 0.35374621882785107 1102 98-99 0.4535169472408853 1102 100-101 0.5369055845124855 1103 1 -0.020520333916756783 1103 2 0.014054552265253761 1103 3 0.047509834872464296 1103 4 -0.012819630910811952 1103 5 -0.12684037403241177 1103 6 -0.06503652230190227 1103 7 -0.002900801470744341 1103 8 0.11408323159136557 1103 9 0.11839951060147058 1103 10-11 0.04587301530130361 1103 12-13 0.15605632187099872 1103 14-15 0.23312635465690335 1103 16-17 0.17877954872563606 1103 18-19 0.17332229550458322 1103 20-21 0.10458952969764823 1103 22-23 0.09476252030457744 1103 24-25 0.11490773316528902 1103 26-27 0.11021066979215988 1103 28-29 -0.10213147174913217 1103 30-31 -0.030634313848892702 1103 32-33 0.06903162674932872 1103 34-35 0.08671708649443843 1103 36-37 0.1531217476710225 1103 38-39 0.1554058756930914 1103 40-41 0.14311694929008922 1103 42-43 -0.02434560973055966 1103 44-45 -0.1349652070670757 1103 46-47 0.051527820436113814 1103 48-49 -0.09312525914319991 1103 50-51 -0.16696740472177396 1103 52-53 -0.19024647242932247 1103 54-55 -0.13866235748680822 1103 56-57 -0.002868123688607227 1103 58-59 0.016645338200468984 1103 60-61 -0.007112987092362744 1103 62-63 0.22489645872410335 1103 64-65 0.12359115470942328 1103 66-67 0.3160710445538726 1103 68-69 0.21861289748486 1103 70-71 0.11039093175982373 1103 72-73 0.22215905435714234 1103 74-75 0.24943895334149602 1103 76-77 0.2214279438800837 1103 78-79 0.32118243568532634 1103 80-81 0.23377638654246624 1103 82-83 0.2982481123007439 1103 84-85 0.2297310892625788 1103 86-87 0.4635686646938524 1103 88-89 0.44826190863753723 1103 90-91 0.5414685006179702 1103 92-93 0.34116095562247395 1103 94-95 0.48987261148522165 1103 96-97 0.6454203822381217 1103 98-99 0.8074881128908054 1103 100-101 0.9291623854645721 1104 1 0.08981639627397797 1104 2 0.1426784403916379 1104 3 0.13294707398450356 1104 4 0.09308053630040547 1104 5 0.08224328420891425 1104 6 0.08218465400673836 1104 7 0.1498274134989117 1104 8 0.17567930921300245 1104 9 0.21466620641876233 1104 10-11 0.14331828702965765 1104 12-13 0.2377966950799575 1104 14-15 0.31071964677449415 1104 16-17 0.3140105066880068 1104 18-19 0.2689634675216084 1104 20-21 0.3629239764228558 1104 22-23 0.40049304708064426 1104 24-25 0.3132429086560933 1104 26-27 0.3263584958439125 1104 28-29 0.3441220658005193 1104 30-31 0.38761640580712964 1104 32-33 0.2344476506852331 1104 34-35 0.33546381211014875 1104 36-37 0.25456227432562173 1104 38-39 0.3005322557929304 1104 40-41 0.2197187153320428 1104 42-43 0.3313972655735995 1104 44-45 0.33513799423822377 1104 46-47 0.34301421883606764 1104 48-49 0.43265279934428946 1104 50-51 0.4256777309703281 1104 52-53 0.4279289414487977 1104 54-55 0.33959816001773646 1104 56-57 0.46631885731896006 1104 58-59 0.5051429879314284 1104 60-61 0.5337152954905449 1104 62-63 0.509486710269691 1104 64-65 0.6287747024213104 1104 66-67 0.6017119331620506 1104 68-69 0.5882500936807062 1104 70-71 0.5554335038165235 1104 72-73 0.6386647852887819 1104 74-75 0.6077713757178387 1104 76-77 0.7281941275371118 1104 78-79 0.7932188901597463 1104 80-81 0.7585989523641707 1104 82-83 0.7056847603029119 1104 84-85 0.7572472536882913 1104 86-87 0.679800298788745 1104 88-89 0.7775841971234456 1104 90-91 0.9212314898524845 1104 92-93 0.9039971493607588 1104 94-95 0.8905062326809663 1104 96-97 1.0587025400320655 1104 98-99 0.9965627020492072 1104 100-101 1.1028992929882655 1105 1 0.2716303736447827 1105 2 0.33590188094720475 1105 3 0.27130891534706336 1105 4 0.30583121819886827 1105 5 0.2976700306519362 1105 6 0.19661468157401174 1105 7 0.3350434178106241 1105 8 0.3026504418745688 1105 9 0.4162157273600542 1105 10-11 0.4131824717922754 1105 12-13 0.5112429862442269 1105 14-15 0.5626156704597705 1105 16-17 0.5066394483160437 1105 18-19 0.6510912638515549 1105 20-21 0.5160129127340838 1105 22-23 0.5655416084478588 1105 24-25 0.5333014663452502 1105 26-27 0.5002445159239599 1105 28-29 0.4879116914755883 1105 30-31 0.5807512632912548 1105 32-33 0.5697485053798559 1105 34-35 0.6517979660280133 1105 36-37 0.6082451633172852 1105 38-39 0.6071004219685889 1105 40-41 0.6473311420894134 1105 42-43 0.580513164641701 1105 44-45 0.578407956838511 1105 46-47 0.5711515890568606 1105 48-49 0.620366518522296 1105 50-51 0.4983946001732775 1105 52-53 0.453607471388608 1105 54-55 0.6034793913315326 1105 56-57 0.7025371340186055 1105 58-59 0.7278116053071884 1105 60-61 0.6979168096875057 1105 62-63 0.7559180779631802 1105 64-65 0.7576783662826969 1105 66-67 0.7777466162112745 1105 68-69 0.9150797483432882 1105 70-71 0.7519673838825085 1105 72-73 0.7887905131630433 1105 74-75 0.792293191006209 1105 76-77 0.8626306372366379 1105 78-79 1.0155310813025302 1105 80-81 0.8942563243329538 1105 82-83 1.0120023811418726 1105 84-85 0.8946174744813717 1105 86-87 0.9134064218545745 1105 88-89 1.073412325935795 1105 90-91 1.064920788143457 1105 92-93 1.1675115757466763 1105 94-95 1.2661084701090886 1105 96-97 1.2256057923872987 1105 98-99 1.2101426780871662 1105 100-101 1.5372125329254835 1106 1 0.08882111180915686 1106 2 0.2224489715112803 1106 3 0.05520065442765798 1106 4 0.035393543108625636 1106 5 0.07325516295974666 1106 6 0.08690727040666246 1106 7 0.15350499052816957 1106 8 0.09490156735896704 1106 9 0.0852523918852981 1106 10-11 0.05127553135326224 1106 12-13 -0.15808324465510992 1106 14-15 -0.06375319813461999 1106 16-17 -0.07068147410696923 1106 18-19 -1.2969807371376874E-4 1106 20-21 0.029768294813251828 1106 22-23 0.045213203687467285 1106 24-25 -0.022093230456391666 1106 26-27 0.1886654277085782 1106 28-29 0.3594775105618595 1106 30-31 0.36757505519437217 1106 32-33 0.37851903686249955 1106 34-35 0.37248537536456894 1106 36-37 0.21091566832763675 1106 38-39 0.24825820502616125 1106 40-41 0.4978489185165458 1106 42-43 0.2532523409001044 1106 44-45 0.40449092889252114 1106 46-47 0.5168996949690907 1106 48-49 0.5575646334647857 1106 50-51 0.3030537839527838 1106 52-53 0.4814942904179702 1106 54-55 0.6459861681536836 1106 56-57 0.5516456324463448 1106 58-59 0.43479094867910106 1106 60-61 0.5158369788462238 1106 62-63 0.7666616192620026 1106 64-65 0.6578470343144787 1106 66-67 0.576824044754666 1106 68-69 0.5890201766334968 1106 70-71 0.5505128927070189 1106 72-73 0.8247912726949238 1106 74-75 0.7495644717121905 1106 76-77 0.7355137823088747 1106 78-79 0.8162090109225453 1106 80-81 0.6798207241419583 1106 82-83 0.5823624742979199 1106 84-85 0.7506701023709503 1106 86-87 0.8496458442804737 1106 88-89 1.0074678880429921 1106 90-91 0.8879312022244044 1106 92-93 0.9340906680854637 1106 94-95 1.1056775656558884 1106 96-97 1.252297853753518 1106 98-99 1.2798650860918563 1106 100-101 1.633041169009104 1107 1 0.04740748602575806 1107 2 -0.10018375283291903 1107 3 -0.030674779505336147 1107 4 0.005452711376143782 1107 5 0.1733878149735233 1107 6 0.14751025644432048 1107 7 -0.00964331299643817 1107 8 0.14935091882259854 1107 9 0.10507568370823606 1107 10-11 0.10811832623250694 1107 12-13 0.13842402821844502 1107 14-15 0.22385128960150524 1107 16-17 0.1959590809467855 1107 18-19 0.21037769290650488 1107 20-21 0.2986636764860364 1107 22-23 0.24806833318270094 1107 24-25 0.19252411386376878 1107 26-27 0.2616064976360448 1107 28-29 0.18430402653816458 1107 30-31 0.172641791403926 1107 32-33 0.0424606961342846 1107 34-35 0.1103424845335681 1107 36-37 -0.05254026738428763 1107 38-39 0.048834807303556715 1107 40-41 0.14534372955183272 1107 42-43 0.10468218455145006 1107 44-45 0.23165926616075438 1107 46-47 0.07544512818471816 1107 48-49 0.12319834903302507 1107 50-51 0.13802330411078145 1107 52-53 -0.025578138691280117 1107 54-55 0.12544006751650727 1107 56-57 0.06520332616405966 1107 58-59 0.1447831092502625 1107 60-61 0.011324254042285986 1107 62-63 0.1737564608940474 1107 64-65 0.12806572584326403 1107 66-67 0.08289692687034034 1107 68-69 0.2054023040040036 1107 70-71 0.2674724546501892 1107 72-73 0.35067932184632156 1107 74-75 0.4274277557194779 1107 76-77 0.37719699249553784 1107 78-79 0.46078623068376956 1107 80-81 0.4180417643287413 1107 82-83 0.5303443167856479 1107 84-85 0.48086583444004205 1107 86-87 0.4683524534579959 1107 88-89 0.5044337754302681 1107 90-91 0.6754163943042819 1107 92-93 0.5133908702539536 1107 94-95 0.5496501228989779 1107 96-97 0.6274035255287629 1107 98-99 0.9686548217509134 1107 100-101 1.213630636193482 1108 1 0.03866553676612128 1108 2 0.041647850517232854 1108 3 0.006682398835447856 1108 4 -0.02634167772627194 1108 5 0.09024787371451737 1108 6 0.07291303313268571 1108 7 0.005525981327295426 1108 8 0.07413403906732441 1108 9 0.23253003742660638 1108 10-11 0.09598491271859899 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-1.6257877495330035 2316 88-89 -1.497887049052462 2316 90-91 -1.5505886407047633 2316 92-93 -1.62846004154612 2316 94-95 -1.7823643794059372 2316 96-97 -1.9604244303788008 2316 98-99 -2.013372706576387 2316 100-101 -2.3001822138271315 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8665.0 3 1492.0 4 1291.0 5 1573.0 6 1801.0 7 2082.0 8 2541.0 9 3242.0 10 4315.0 11 5561.0 12 7179.0 13 9414.0 14 10770.0 15 13242.0 16 16562.0 17 21210.0 18 18359.0 19 13214.0 20 13155.0 21 13841.0 22 15294.0 23 16839.0 24 19503.0 25 21974.0 26 26090.0 27 31167.0 28 37274.0 29 45121.0 30 55796.0 31 69735.0 32 88056.0 33 115394.0 34 157435.0 35 220413.0 36 322957.0 37 465796.0 38 410001.0 39 57950.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.751843172064596 19.831689090875663 15.143107457904101 22.27336027915564 2 14.471502041430437 20.77463223476792 41.171155306009396 23.582710417792246 3 19.076467357271547 25.17667274347669 30.15838888307092 25.58847101618084 4 11.86924556529173 17.004872836076085 39.04821543064757 32.07766616798462 5 12.897316809336226 38.37431494891206 34.80651460452203 13.921853637229681 6 30.567900264339308 37.469039755111346 17.9727665165085 13.990293464040843 7 27.593427105713907 33.334458146279786 21.325812748544507 17.746301999461796 8 25.446272208962007 35.186991814356226 20.23290044473452 19.133835531947245 9 25.59292374074047 17.520780681323593 21.19371084601858 35.69258473191736 10-11 24.19330345920379 27.313449189026525 28.116723112268904 20.37652423950079 12-13 25.46764387707482 24.686017938780893 28.394255916497848 21.452082267646443 14-15 22.038126871327414 26.169893946813033 27.28336378850522 24.50861539335433 16-17 21.10376477211423 29.729308361570865 27.60319603477093 21.56373083154397 18-19 20.92620708652842 28.48628114209572 30.034935094608763 20.552576676767096 20-21 21.986458990010817 27.127336063403224 29.78208558205329 21.104119364532668 22-23 21.3702953196162 27.355145697289014 29.780789393940367 21.493769589154425 24-25 21.62502890695516 27.373664060930203 29.846796874482852 21.15451015763179 26-27 21.354140972653347 27.48595785466388 29.996427130657032 21.163474042025744 28-29 20.895852606994982 28.08545162086689 29.978135872801094 21.040559899337026 30-31 21.15609812877025 27.505165824294696 29.839296145093723 21.49943990184133 32-33 21.054094027818564 27.569468991109808 29.839314413861228 21.5371225672104 34-35 21.837880871977642 27.513457677640595 29.501886007538904 21.146775442842856 36-37 21.53348720125805 27.193002312289817 29.924891154957585 21.348619331494547 38-39 21.238572788134 27.741265695100537 29.89296487530292 21.127196641462547 40-41 21.45397234018565 27.700726685092874 29.655764112620815 21.189536862100656 42-43 21.21802360279455 27.7178135994614 29.220848131610914 21.843314666133136 44-45 21.42804514022442 27.65882017013972 29.110259361304397 21.80287532833147 46-47 21.695508609233908 27.985492864841017 28.884736224296848 21.43426230162823 48-49 21.69599998633226 28.00485802741621 28.772536555332245 21.52660543091929 50-51 21.26252431820915 28.195026644577293 28.9518945297191 21.59055450749446 52-53 21.7195284357836 28.427197281425244 28.32685265380356 21.526421628987595 54-55 21.317792700430594 27.992639771717588 28.643013977171762 22.046553550680063 56-57 21.692460801690387 28.250369547611378 28.394366606176803 21.66280304452144 58-59 22.148135733980357 27.84613200427411 28.407957702953556 21.597774558791976 60-61 21.72284236365589 27.947977724015814 29.107105225905922 21.22207468642237 62-63 21.470883257600892 28.120733778376177 28.93297326796473 21.475409696058197 64-65 21.27147434693048 28.337918000362105 28.64922984426081 21.741377808446604 66-67 21.402078260178836 28.28159605461967 28.285930745729154 22.030394939472338 68-69 21.35332219962363 28.137056507348717 28.64870612258667 21.860915170440983 70-71 21.58508221219965 28.129795498127546 28.48101252308939 21.804109766583416 72-73 21.583378907713964 27.855965453302613 28.83441412772344 21.726241511259985 74-75 21.278334824794428 28.578956797165777 28.370237653702162 21.772470724337634 76-77 21.453293773513913 28.072167770762608 28.387786453851128 22.08675200187236 78-79 21.48075102252786 28.18386581940388 28.43351337454052 21.901869783527747 80-81 21.601138439300495 28.38036085451271 28.286159913143827 21.732340793042965 82-83 21.528030614765974 28.196748686570423 28.594466459205687 21.680754239457915 84-85 21.420918820499157 28.282011143222174 28.32419961466724 21.97287042161143 86-87 21.485505606366377 28.223543237216468 28.377249656461135 21.91370149995602 88-89 21.516550700091347 28.11979127297605 28.070615978746368 22.293042048186237 90-91 21.32990534015772 27.932500334692673 28.519958039040432 22.217636286109176 92-93 21.741385521188487 27.893079269796523 28.587998675245824 21.77753653376917 94-95 21.457291303664526 27.815850296209742 28.523341464581243 22.20351693554449 96-97 21.117560722931593 27.901674094894275 28.813452202056332 22.1673129801178 98-99 21.22174246246795 28.120738027614433 28.157679125354385 22.49984038456323 100-101 21.12260065065798 27.58423902948713 28.8938173827904 22.39934293706449 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3228.0 1 1835.0 2 436.0 3 434.0 4 394.0 5 407.5 6 627.0 7 1220.0 8 1995.5 9 2390.5 10 2301.0 11 2177.0 12 2316.0 13 2508.5 14 2702.0 15 2845.0 16 2969.5 17 3212.5 18 3399.5 19 3686.5 20 4224.0 21 4889.5 22 5911.5 23 7340.5 24 9127.0 25 11569.5 26 14800.0 27 18428.0 28 21795.0 29 25684.5 30 31138.5 31 37039.5 32 43161.0 33 49336.5 34 55998.0 35 61693.0 36 66939.0 37 73169.0 38 77120.0 39 78600.5 40 79373.0 41 81966.0 42 85516.0 43 88207.0 44 89945.5 45 91190.5 46 89733.5 47 93201.5 48 108180.5 49 106781.5 50 89479.0 51 79594.0 52 73599.0 53 69078.5 54 63767.5 55 56094.5 56 48573.0 57 41816.5 58 36005.5 59 31045.5 60 26175.5 61 20884.5 62 15757.0 63 11428.0 64 8245.0 65 6157.0 66 4454.5 67 3296.5 68 2354.5 69 1615.5 70 1261.5 71 1004.5 72 811.0 73 643.5 74 505.0 75 414.5 76 275.0 77 156.0 78 98.5 79 60.0 80 35.5 81 19.0 82 10.0 83 6.5 84 8.5 85 5.5 86 5.0 87 5.5 88 4.5 89 5.5 90 5.5 91 5.5 92 4.5 93 4.0 94 2.5 95 1.0 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32156848705542995 2 0.2887936472216824 3 0.2905410704117782 4 0.2903279700227421 5 0.2780107675364572 6 0.21322824926949188 7 0.22085724319698316 8 0.2190671999290802 9 0.2162968948716113 10-11 0.22450125984949998 12-13 0.22565200195029475 14-15 0.20851873067179472 16-17 0.2199622215630317 18-19 0.2057271155754222 20-21 0.21224798747992596 22-23 0.21026615386189046 24-25 0.20233881938974868 26-27 0.21550842343217783 28-29 0.2145494716815155 30-31 0.2218161949476455 32-33 0.2237340984489701 34-35 0.21269549829690168 36-37 0.20926458203342096 38-39 0.200207815499388 40-41 0.20116676725005028 42-43 0.19956851433227976 44-45 0.2181508682562251 46-47 0.20643034685924122 48-49 0.2144855415648047 50-51 0.2223276358813321 52-53 0.22927470856390794 54-55 0.22810265642420954 56-57 0.19594580771866657 58-59 0.18345812492115288 60-61 0.1921100007160173 62-63 0.1924296512995714 64-65 0.18957410608648806 66-67 0.20193392865058016 68-69 0.19072484818728286 70-71 0.19594580771866657 72-73 0.20890231137205964 74-75 0.1981194316868345 76-77 0.20794335962139732 78-79 0.20504519433050677 80-81 0.19219524087163173 82-83 0.20574842561432582 84-85 0.18923314546403036 86-87 0.19221655091053533 88-89 0.19984554483802666 90-91 0.19654248880796757 92-93 0.20225357923413426 94-95 0.201507727872508 96-97 0.19473113550116097 98-99 0.20229619931194145 100-101 0.18646284040656144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2346312.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.4543163558589 #Duplication Level Percentage of deduplicated Percentage of total 1 75.16442251284663 40.17862819707146 2 13.786093085701397 14.73852362228803 3 4.77976998474761 7.664980106188128 4 2.220153216248131 4.747070895192208 5 1.1646713842647691 3.1128356312552534 6 0.6786827534466873 2.1767113564802765 7 0.4245341983135237 1.5885229738372426 8 0.3042574703171304 1.301110005757219 9 0.21123627057885933 1.016234139001874 >10 1.1154041941945934 10.950773582588624 >50 0.08965523020083321 3.3269280830610133 >100 0.05649663002477797 5.311672247748211 >500 0.002949199263113594 1.0950957449510779 >1k 0.0013550374992681673 1.5861887943752755 >5k 3.1883235276898054E-4 1.204724620204172 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8718 0.37156183832329204 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8345 0.3556645493012012 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6093 0.25968413407935514 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5119 0.2181721782951287 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4534 0.19323943277790848 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3976 0.16945742936148306 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3458 0.14738022905734618 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3205 0.136597349372121 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2619 0.11162198377709359 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2465 0.10505849179478263 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2382 0.10152102533678385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.131003890360702E-5 0.0 22-23 0.0 0.0 0.0 4.262007780721404E-5 0.0 24-25 0.0 0.0 0.0 1.065501945180351E-4 0.0 26-27 0.0 0.0 0.0 2.3441042793967724E-4 0.0 28-29 2.131003890360702E-5 0.0 0.0 5.114409336865685E-4 0.0 30-31 4.262007780721404E-5 0.0 0.0 0.001832663345710204 0.0 32-33 4.262007780721404E-5 0.0 0.0 0.003516156419095159 0.0 34-35 4.262007780721404E-5 0.0 0.0 0.0053914398426125765 0.0 36-37 4.262007780721404E-5 0.0 0.0 0.007756854160912957 0.0 38-39 4.262007780721404E-5 0.0 0.0 0.013340084353657997 0.0 40-41 4.262007780721404E-5 0.0 0.0 0.019242965129957143 0.0 42-43 4.262007780721404E-5 0.0 0.0 0.02557204668432843 0.0 44-45 4.262007780721404E-5 0.0 0.0 0.030750386137904935 0.0 46-47 4.262007780721404E-5 0.0 0.0 0.038443310182107074 0.0 48-49 4.262007780721404E-5 0.0 0.0 0.04481501181428557 0.0 50-51 4.262007780721404E-5 0.0 0.0 0.05171946441905424 0.0 52-53 4.262007780721404E-5 0.0 0.0 0.06940679670904806 0.0 54-55 4.262007780721404E-5 0.0 0.0 0.0980261789565923 0.0 56-57 4.262007780721404E-5 0.0 0.0 0.13938896446849353 0.0 58-59 4.262007780721404E-5 0.0 0.0 0.18695297130134442 0.0 60-61 4.262007780721404E-5 0.0 0.0 0.25084046793435827 0.0 62-63 4.262007780721404E-5 0.0 0.0 0.2942063971031985 0.0 64-65 4.262007780721404E-5 0.0 0.0 0.3444980889157111 0.0 66-67 4.262007780721404E-5 0.0 0.0 0.44361107985638737 0.0 68-69 4.262007780721404E-5 0.0 0.0 0.571663103628162 0.0 70-71 4.262007780721404E-5 0.0 0.0 0.7019100614070081 0.0 72-73 4.262007780721404E-5 0.0 0.0 0.8501000719426913 0.0 74-75 4.262007780721404E-5 0.0 0.0 1.0094139227860575 0.0 76-77 4.262007780721404E-5 0.0 0.0 1.158626815189114 0.0 78-79 4.262007780721404E-5 0.0 0.0 1.3074561268919052 0.0 80-81 4.262007780721404E-5 0.0 0.0 1.4674092789023794 0.0 82-83 4.262007780721404E-5 0.0 0.0 1.6715381415600312 0.0 84-85 4.262007780721404E-5 0.0 0.0 1.9632086440337004 0.0 86-87 4.262007780721404E-5 0.0 0.0 2.314696425709795 0.0 88-89 4.262007780721404E-5 0.0 0.0 2.7149841964751493 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9785 0.0 24.6372 1 TACATGG 10050 0.0 23.648283 2 ACATGGG 10110 0.0 22.567621 3 GAGTACT 8060 0.0 21.923105 12-13 AGTACTT 8605 0.0 20.838202 12-13 GTACTTT 8730 0.0 20.212507 14-15 AGAGTAC 13800 0.0 20.187742 10-11 GTATCAA 19325 0.0 20.028532 1 CTACCGT 120 0.0047452273 19.80586 4 CATGGGA 6345 0.0 18.65403 4 TACTTTT 10050 0.0 17.723145 14-15 CATGGGG 4080 0.0 17.70877 4 CAACGCA 21650 0.0 17.345364 5 GTATAGG 795 0.0 17.345032 1 TATCAAC 21900 0.0 17.320263 2 ATCAACG 21735 0.0 17.298662 3 TCAACGC 21715 0.0 17.293074 4 AACGCAG 21885 0.0 17.148117 6 GAGTACA 8430 0.0 16.752249 1 ACTTTTT 10820 0.0 16.593582 16-17 >>END_MODULE