FastQCFastQC Report
Thu 26 May 2016
SRR937233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346312
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109290.4657948303550423No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT96870.41286069371848244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83960.3578381732693691No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63890.2722996771102905No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42400.18070912990258756No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40760.17371943714220445No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31040.1322927215135924No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30240.12888311528901525No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24790.10565517288408362No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24670.10514373195039704No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23530.10028504308037464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1751.4868237E-724.4297732
GTACATG94650.023.8522111
TACATGG97500.022.5575182
GAGTACT98100.021.86129812-13
AGTACTT103200.021.77051212-13
ACATGGG98450.021.2300933
GTACTTT104650.020.37866814-15
AGAGTAC160000.020.0684510-11
TACTTTT117550.018.88981214-15
CATGGGA58600.018.4010734
GTATCAA227200.018.3462331
TACCGCG800.001192216917.81147816-17
ATAACGC1901.8660205E-417.500863
ACTTTTT124250.017.33602716-17
GTATAGC9700.017.149521
ACCGTGC2504.5649485E-617.0997458
CATGGGG39200.016.6015824
GTCTAGG8350.016.5069641
CAACGCA255850.016.1157045
ATGGGGA23650.016.0684415
Produced by FastQC (version 0.11.5)