FastQCFastQC Report
Thu 26 May 2016
SRR937232_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937232_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451962
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90330.3683988577310741No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86070.35102501588523805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64150.26162721934516114No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53280.2172953740718657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45920.18727859567154792No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42570.17361606745944674No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35720.14567925604067272No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32580.13287318482097193No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT28490.11619266530231709No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25830.1053442100652457No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24610.1003686027760626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA804.494154E-429.6885769
GTACATG101100.025.3346961
TACATGG102750.024.3221572
ACATGGG103400.022.6524753
GAGTACT80300.022.1818812-13
CGTATAG1553.895955E-521.460731
TAGCCCT16200.020.824194
AGTACTT84550.020.56128112-13
GTACTTT88200.020.2750214-15
AGAGTAC138250.019.77255810-11
GTGTAGC12550.019.6896381
GTATCAA206150.019.3861141
CATGGGG42500.019.1175384
GTACCGT2251.6666654E-619.003026
CATGGGA60150.018.3263994
TAAGGTG8900.018.1522925
AGTACAT78800.017.9675222
GAGTACA87550.017.8573861
TGTACCG2403.082885E-617.8186115
TACGACC1600.001189124417.8178834
Produced by FastQC (version 0.11.5)