FastQCFastQC Report
Thu 26 May 2016
SRR937232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451962
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112460.4586531112635514No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT99600.4062053163956048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87100.3552257335146303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65580.26745928362674465No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44320.1807532090627832No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42220.17218863913877946No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31380.1279791448643984No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31210.12728582253721715No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC26050.10624145072395087No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25380.10350894508153062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG97950.024.2142851
TACATGG102250.022.6238522
ACATGGG99350.022.1367573
GAGTACT99700.021.87159312-13
AGTACTT103800.021.39671512-13
GTACTTT105450.020.4270214-15
AGAGTAC162800.020.16445510-11
GTATCAA231950.019.180371
CATGGGG41950.018.2303434
TACTTTT122700.017.86493714-15
CAACGCA258150.017.130835
ATCAACG258050.017.1006553
TCAACGC258550.017.0859574
AACGCAG262650.016.8724676
CATGGGA60100.016.8347244
TATCAAC263550.016.8158762
GTGTAGC12450.016.7980481
ACTTTTT129050.016.76504316-17
ACCGTGC2858.5778265E-716.6693258
CTAGGAC13300.016.4288463
Produced by FastQC (version 0.11.5)