FastQCFastQC Report
Thu 26 May 2016
SRR937231_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937231_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672498
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94470.5648437247757546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69470.4153667149377757No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT67370.40281064611138545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51330.3069061965993382No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT41660.249088489194008No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31240.18678647149353841No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26440.15808688560464645No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26060.15581483505510918No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20900.12496278022455035No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT19170.11461897114376221No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC18870.11282524702570645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG2050.031.28091294-95
GTACATG90950.027.4398481
TACATGG94100.026.0517982
ACATGGG93650.025.2115883
GTGTAGC7750.023.9214781
GAGTACT72050.022.51418512-13
AGTACTT76100.022.09622612-13
GTATCAA167700.021.9681641
CATGGGG31950.021.2638954
CATGGGA61150.021.1324624
CATTCCG2056.8242844E-720.8564439
GTACTTT77400.020.8339814-15
TAGCCCG1150.003719154320.656795
CGCACCA1404.845692E-420.3616945
AGAGTAC130850.019.98642710-11
CAACGCA187400.019.3946745
TCAACGC187600.019.3734194
ATCAACG189350.019.2183043
GTATAGG5950.019.1742951
AACGCAG191000.019.1257516
Produced by FastQC (version 0.11.5)