FastQCFastQC Report
Thu 26 May 2016
SRR937231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937231_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672498
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113460.6783864614486833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95610.5716598764243664No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78200.46756408677319794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68960.41231738393708095No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34810.2081317884984018No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32030.19150994500441854No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23220.13883424673751477No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22910.13698073181552384No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22760.13608386975649597No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19840.11862495500742003No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT19730.11796725616413292No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC18910.11306441024144721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG90850.024.0618021
GAGTACT97750.023.37516812-13
TACATGG94200.022.9939942
AGTACTT102850.022.40081812-13
GTACTTT102850.022.12104814-15
ACATGGG93450.021.856953
GTATCAA202200.020.6586991
TGCGCCG1150.003723637420.6524985
AGAGTAC161200.020.58390410-11
TACTTTT113350.019.9671414-15
GGTATCA158550.019.8120981
CATGGGG30650.019.3722464
GTGTAAG9000.019.0088231
AGGCGAG1759.934451E-519.0014367
ACTTTTT120500.018.92032816-17
CTACACG3305.984475E-1018.7124164
TATCACG1559.626E-418.3873862
CATGGGA61850.018.3551794
TACACGG3651.1641532E-1018.2194655
GGACCGA2403.0931078E-617.8117166
Produced by FastQC (version 0.11.5)