FastQCFastQC Report
Thu 26 May 2016
SRR937230_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937230_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624093
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90540.5574803905933958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68870.42405207090973235No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64580.39763732741905794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49920.30737156061875764No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40270.2479537809719025No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30200.18594994252176447No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25080.15442465425317395No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22340.13755369920318603No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT19500.12006701586670221No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT19480.11994387020940304No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19250.11852769515046245No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC17020.10479695436160366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG90600.026.3475151
TACATGG93300.024.8082962
ACATGGG92100.024.2535023
GAGTACT73550.022.80030312-13
GTGTAGC8050.022.4466691
GTATCAA169650.021.4983771
AGTACTT77900.021.4356412-13
GTACTTT78350.021.30956814-15
GTATAGG5650.021.0404831
ACAGCGT1604.9860413E-520.7838828
CATGGGA60500.020.5069854
CATGGGG31900.019.8188114
AGAGTAC134850.019.62243310-11
GTACCCG1250.005994555519.0205961
TCAACGC191700.018.8951154
ATCAACG191950.018.8670123
CAACGCA193000.018.743795
AACGCAG196350.018.4604476
TATCAAC196600.018.4213352
TACTTTT90350.018.4004414-15
Produced by FastQC (version 0.11.5)