##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937230_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1624093 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.783724823640025 33.0 31.0 34.0 30.0 34.0 2 31.889140584929557 33.0 31.0 34.0 30.0 34.0 3 31.921829599659624 33.0 31.0 34.0 30.0 34.0 4 35.548741974751444 37.0 35.0 37.0 33.0 37.0 5 35.56795516020326 37.0 35.0 37.0 33.0 37.0 6 35.627662947873056 37.0 35.0 37.0 33.0 37.0 7 35.575066821912294 37.0 35.0 37.0 33.0 37.0 8 35.57078135303828 37.0 35.0 37.0 33.0 37.0 9 37.189861048597585 39.0 37.0 39.0 34.0 39.0 10-11 37.072410570084344 39.0 37.0 39.0 33.0 39.0 12-13 36.94851711078122 39.0 37.0 39.0 33.0 39.0 14-15 38.09732755451812 40.0 38.0 41.0 33.0 41.0 16-17 38.075546474247474 40.0 38.0 41.0 33.0 41.0 18-19 38.08812364809158 40.0 38.0 41.0 33.5 41.0 20-21 38.039653825242766 40.0 38.0 41.0 33.0 41.0 22-23 37.89309048188743 40.0 38.0 41.0 33.0 41.0 24-25 37.72726253976835 40.0 37.0 41.0 32.5 41.0 26-27 37.57132073101725 40.0 37.0 41.0 32.0 41.0 28-29 37.64466443731978 40.0 37.0 41.0 33.0 41.0 30-31 37.49337722655045 40.0 37.0 41.0 32.5 41.0 32-33 37.283982813792065 39.5 36.5 41.0 32.0 41.0 34-35 37.150043747494756 39.0 36.0 41.0 31.5 41.0 36-37 37.13255737202242 40.0 36.0 41.0 31.0 41.0 38-39 37.04268382414061 40.0 36.0 41.0 31.0 41.0 40-41 36.88196427175045 39.5 36.0 41.0 31.0 41.0 42-43 36.62625323796112 39.0 36.0 41.0 30.0 41.0 44-45 36.3676926136619 39.0 36.0 41.0 30.0 41.0 46-47 35.98080651785335 39.0 35.0 41.0 29.5 41.0 48-49 35.63885534880084 39.0 35.0 41.0 27.5 41.0 50-51 34.75914679762796 38.0 34.0 39.5 26.0 40.5 52-53 34.87117578857861 38.0 34.5 40.0 26.5 40.5 54-55 35.212739664538915 39.0 35.0 40.0 26.5 41.0 56-57 35.0221301982091 38.5 35.0 40.0 26.0 41.0 58-59 34.798076218541674 38.0 34.5 40.0 26.0 41.0 60-61 34.50462196438258 38.0 34.0 40.0 25.0 41.0 62-63 34.20401972054556 38.0 34.0 40.0 23.5 41.0 64-65 33.85555014398806 37.0 34.0 40.0 23.0 41.0 66-67 33.471618004633974 37.0 33.0 39.5 22.0 41.0 68-69 33.00915064592976 36.0 32.5 39.0 20.5 41.0 70-71 32.56569851603326 36.0 32.0 39.0 19.5 40.0 72-73 32.177470132560146 35.0 32.0 38.5 18.5 40.0 74-75 31.730640117283926 35.0 31.0 37.5 17.0 39.5 76-77 31.287845893061544 35.0 31.0 37.0 12.5 39.0 78-79 30.800102888196676 35.0 31.0 36.5 7.5 39.0 80-81 30.304706688594802 34.0 30.0 36.0 6.5 37.5 82-83 29.821557324611337 34.0 30.0 35.5 2.0 37.0 84-85 29.373688575715796 34.0 29.0 35.0 2.0 37.0 86-87 28.947276110419786 34.0 29.0 35.0 2.0 36.0 88-89 28.525331369570587 34.0 29.0 35.0 2.0 36.0 90-91 28.083753208714032 34.0 27.5 35.0 2.0 35.5 92-93 27.626670086011085 33.5 27.0 35.0 2.0 35.0 94-95 27.09481445951679 33.0 26.0 35.0 2.0 35.0 96-97 26.45133068118636 33.0 25.0 35.0 2.0 35.0 98-99 25.68629936832435 32.0 23.5 34.5 2.0 35.0 100-101 23.715268460611554 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05220297435207755 1101 2 -0.052000763906086434 1101 3 -0.07847685364432522 1101 4 -0.09854268621667472 1101 5 -0.05844588320606192 1101 6 -0.05393529430828892 1101 7 -0.015462547484659694 1101 8 0.03227080804659721 1101 9 0.01530481127949912 1101 10-11 0.1286405217556208 1101 12-13 0.008114616179192069 1101 14-15 0.11393739151601068 1101 16-17 0.2511863961816161 1101 18-19 0.27173318718562456 1101 20-21 0.17838533269529933 1101 22-23 0.31575536699248374 1101 24-25 0.2945172410943826 1101 26-27 0.19486878350594594 1101 28-29 0.15677246247287968 1101 30-31 0.22044357272672244 1101 32-33 0.280437394277925 1101 34-35 0.12404335169767933 1101 36-37 0.2921775457194542 1101 38-39 0.21894048127591503 1101 40-41 0.2549099683016607 1101 42-43 0.397185407739002 1101 44-45 0.4951557978878611 1101 46-47 0.5796959525130276 1101 48-49 0.4279391041481304 1101 50-51 0.37692683230679336 1101 52-53 0.285683605517427 1101 54-55 0.3902345069366291 1101 56-57 0.34522155700108215 1101 58-59 0.5028633670035916 1101 60-61 0.3884325157948538 1101 62-63 0.4191566036164929 1101 64-65 0.3893468325027811 1101 66-67 0.42099640830877405 1101 68-69 0.624796050233158 1101 70-71 0.6398517158135562 1101 72-73 0.5249346752011235 1101 74-75 0.36150039881732 1101 76-77 0.4252878097914916 1101 78-79 0.4676005534001355 1101 80-81 0.45181654708119723 1101 82-83 0.3654337403382151 1101 84-85 0.3292951657620513 1101 86-87 0.3564272329510132 1101 88-89 0.5250162247220302 1101 90-91 0.6368195857631243 1101 92-93 0.8522257368301744 1101 94-95 1.0293697800162498 1101 96-97 1.0104922417014244 1101 98-99 0.9315510199324386 1101 100-101 1.198019487438998 1102 1 0.1036104213425908 1102 2 0.15556426006476798 1102 3 0.18586925177031688 1102 4 -0.007935719678783926 1102 5 0.1693611621401061 1102 6 -0.05382650461504568 1102 7 0.12917092262101448 1102 8 0.03001934680970919 1102 9 0.1682882472634759 1102 10-11 0.2896080076286651 1102 12-13 0.29557267789521546 1102 14-15 0.3876051460796006 1102 16-17 0.3141584963366668 1102 18-19 0.47212851351187624 1102 20-21 0.37984314313853673 1102 22-23 0.4153017910667032 1102 24-25 0.2254717635761594 1102 26-27 0.36209881517202547 1102 28-29 0.2851822769593255 1102 30-31 0.2148182033129089 1102 32-33 0.36852306687520553 1102 34-35 0.28228438511411014 1102 36-37 0.4094838158319831 1102 38-39 0.4680521748830202 1102 40-41 0.5408873765942346 1102 42-43 0.5393031518804889 1102 44-45 0.4641939942061484 1102 46-47 0.38229990948827464 1102 48-49 0.43252626011143036 1102 50-51 0.2515105903767889 1102 52-53 0.3983800194550682 1102 54-55 0.30652083798280927 1102 56-57 0.23555883360386787 1102 58-59 0.38636759438261237 1102 60-61 0.515581683310991 1102 62-63 0.16330594531034848 1102 64-65 0.2389066768278738 1102 66-67 0.1758878042147387 1102 68-69 0.25183228776589317 1102 70-71 0.15418511482045716 1102 72-73 0.13410127762190882 1102 74-75 0.3022472786089878 1102 76-77 0.459171087873365 1102 78-79 0.3829833018104054 1102 80-81 0.6459056404147603 1102 82-83 0.6418393688485402 1102 84-85 0.599462090839932 1102 86-87 0.5233772953409037 1102 88-89 0.4721135957805416 1102 90-91 0.5991707851623538 1102 92-93 0.7008582989273862 1102 94-95 0.6828557615648343 1102 96-97 0.836644598668677 1102 98-99 1.2081383929040541 1102 100-101 1.5314114986777412 1103 1 0.05945458484646693 1103 2 -0.039076832949366036 1103 3 0.02261604253054017 1103 4 -0.06171381987961411 1103 5 -0.01636070669465539 1103 6 0.09053517147364687 1103 7 -0.011056267668543285 1103 8 0.05379912793557651 1103 9 0.07111190643085763 1103 10-11 0.19929278994887767 1103 12-13 0.08353312602393004 1103 14-15 0.1442782710372157 1103 16-17 -0.01851175630341828 1103 18-19 0.005235659212694088 1103 20-21 0.016992323761300554 1103 22-23 -2.08723561769375E-4 1103 24-25 -0.020184814591281963 1103 26-27 0.17558263553127063 1103 28-29 0.0888741191646929 1103 30-31 -0.049618878483265405 1103 32-33 0.007100591432923409 1103 34-35 0.0816676035842292 1103 36-37 0.19551695069820596 1103 38-39 0.08530790256137522 1103 40-41 0.12487700794083167 1103 42-43 0.018188963988457374 1103 44-45 0.30071135344341826 1103 46-47 0.18754572265788028 1103 48-49 0.2405074101590614 1103 50-51 0.29972586084352315 1103 52-53 0.2616485634495902 1103 54-55 0.21010179811539587 1103 56-57 0.23545488166063677 1103 58-59 0.2632458806647904 1103 60-61 0.17993221221258437 1103 62-63 0.01019225284886005 1103 64-65 0.12236357289656752 1103 66-67 0.16961294053186293 1103 68-69 0.3179047326861024 1103 70-71 0.237674597242993 1103 72-73 0.33945458152430774 1103 74-75 0.3499989676437565 1103 76-77 0.4742339456228706 1103 78-79 0.36032325268021026 1103 80-81 0.03349318859390493 1103 82-83 0.021596460435365117 1103 84-85 0.15623592385035323 1103 86-87 0.2907797353363044 1103 88-89 0.36826368764162254 1103 90-91 0.6400939373981309 1103 92-93 0.6260179130813661 1103 94-95 0.559580552849976 1103 96-97 0.599823493327758 1103 98-99 0.598638364888636 1103 100-101 0.9363228962342092 1104 1 -0.010709824493908116 1104 2 9.857369891612677E-4 1104 3 0.08493071470621061 1104 4 0.023876691335786404 1104 5 0.00963104760850797 1104 6 -0.016956599154688945 1104 7 -0.057929403259500134 1104 8 0.06698422869158094 1104 9 0.05762078287303751 1104 10-11 0.08471411300032372 1104 12-13 0.14285821366043194 1104 14-15 0.2602769412776098 1104 16-17 0.19821397780996364 1104 18-19 0.1226264215389321 1104 20-21 0.05797875012141418 1104 22-23 0.13417832552951126 1104 24-25 0.1253579677525991 1104 26-27 0.11171751708472044 1104 28-29 0.03645339272414816 1104 30-31 0.061037452864333375 1104 32-33 0.06494661833087179 1104 34-35 0.24202874610892877 1104 36-37 0.0869579589409355 1104 38-39 0.12273974496111606 1104 40-41 0.20413542950431207 1104 42-43 0.049348081004339406 1104 44-45 0.08780170289118416 1104 46-47 0.267527646238257 1104 48-49 0.21120906612531343 1104 50-51 0.13816971560291336 1104 52-53 0.22497183453949532 1104 54-55 0.29987600612185616 1104 56-57 0.36402675546407437 1104 58-59 0.201903743610508 1104 60-61 0.18348753822630215 1104 62-63 0.01069803641267697 1104 64-65 0.05959046016085523 1104 66-67 0.21584019288639666 1104 68-69 0.40626043150743385 1104 70-71 0.3339652209536794 1104 72-73 0.22564081316961904 1104 74-75 0.0608998797757323 1104 76-77 0.22593962945471446 1104 78-79 0.1778345244706081 1104 80-81 0.20909017051451784 1104 82-83 0.314665236908116 1104 84-85 0.6091863279726084 1104 86-87 0.5219522093124915 1104 88-89 0.2831911296649956 1104 90-91 0.3005540698398157 1104 92-93 0.6407776510474861 1104 94-95 0.6827465679937994 1104 96-97 0.6451231789939591 1104 98-99 0.861029765380767 1104 100-101 1.0367454214120784 1105 1 0.03734604162670152 1105 2 0.12492798526031024 1105 3 0.1392725448380041 1105 4 0.09670363490415923 1105 5 0.019292527847170504 1105 6 0.05933422967750346 1105 7 0.12641835936015156 1105 8 0.11028596129773405 1105 9 0.19908513320529408 1105 10-11 0.11614408385663211 1105 12-13 0.03425206480414289 1105 14-15 0.13370066236200984 1105 16-17 0.05663486837080001 1105 18-19 0.23334754605151176 1105 20-21 0.16659485180431943 1105 22-23 0.2750782299522143 1105 24-25 0.3295762471139696 1105 26-27 0.2809632225154317 1105 28-29 0.22691309568957507 1105 30-31 -0.03206958134112625 1105 32-33 -0.025146217413400507 1105 34-35 0.083609906433594 1105 36-37 0.059398431235727855 1105 38-39 0.1349293939611087 1105 40-41 0.021549166395608665 1105 42-43 0.1311790150529788 1105 44-45 -0.04944596656398659 1105 46-47 0.08082950853276571 1105 48-49 0.18529612802375794 1105 50-51 0.06668972123681272 1105 52-53 0.1898517778144111 1105 54-55 0.10246184095741029 1105 56-57 0.3193313255306194 1105 58-59 0.2875755639312203 1105 60-61 0.2528555729441777 1105 62-63 0.23958484894756538 1105 64-65 0.30145063139169537 1105 66-67 0.15204559027410625 1105 68-69 0.24321277345277537 1105 70-71 0.15505079686494128 1105 72-73 0.2196677599952892 1105 74-75 0.18598459190229022 1105 76-77 0.19248063917198976 1105 78-79 0.3184218196572708 1105 80-81 0.2884552163683942 1105 82-83 0.319933298197018 1105 84-85 0.4027621403117614 1105 86-87 0.42822523464906936 1105 88-89 0.3557927774308425 1105 90-91 0.5441469377045784 1105 92-93 0.7683374501934139 1105 94-95 0.7816687673513307 1105 96-97 0.8768663019677696 1105 98-99 0.8187527215150396 1105 100-101 0.9366783453926928 1106 1 0.07755414938095129 1106 2 0.029837701064909794 1106 3 0.01991672065286565 1106 4 0.018567022459301086 1106 5 -0.011980657222764535 1106 6 0.016766560730914648 1106 7 0.010042647900050383 1106 8 -0.1227938952108758 1106 9 0.13848961398237236 1106 10-11 -0.20045044528715295 1106 12-13 -0.3446169449861074 1106 14-15 -0.2668811206278079 1106 16-17 -0.19664301670803042 1106 18-19 0.0017867347090501084 1106 20-21 0.013436649071920215 1106 22-23 0.04045416217320508 1106 24-25 0.024140496018844715 1106 26-27 -0.09012516052413844 1106 28-29 0.06037417089385855 1106 30-31 0.055842310535105355 1106 32-33 0.2682999556344683 1106 34-35 0.2405857116135337 1106 36-37 0.1536647644355824 1106 38-39 0.07389362719291626 1106 40-41 0.03148611415491587 1106 42-43 0.014164434958992445 1106 44-45 0.2233160579758291 1106 46-47 0.42759867451213296 1106 48-49 0.37142734281709266 1106 50-51 0.31975210163808754 1106 52-53 0.09789754062292388 1106 54-55 0.42348178175016216 1106 56-57 0.2906381439839336 1106 58-59 0.3825535563323328 1106 60-61 0.2390285507282428 1106 62-63 0.16848725957365218 1106 64-65 0.3846168012667235 1106 66-67 0.6220418599566315 1106 68-69 0.21890263530360698 1106 70-71 0.4368046067423492 1106 72-73 0.5232135048052555 1106 74-75 0.3863330628066066 1106 76-77 0.6280990547714076 1106 78-79 0.5178714783698553 1106 80-81 0.6238889637319289 1106 82-83 0.5315872432387074 1106 84-85 0.5948366969662366 1106 86-87 0.7010597311979687 1106 88-89 0.7231803096331006 1106 90-91 0.6585095201036602 1106 92-93 0.8101558700615143 1106 94-95 0.8808327748527383 1106 96-97 1.00307208488368 1106 98-99 1.189159230552697 1106 100-101 0.9973788295694206 1107 1 0.08653142189066187 1107 2 0.05152049133930703 1107 3 0.1276781333782928 1107 4 0.15563332357668713 1107 5 0.15042935147562275 1107 6 0.18784304847116573 1107 7 0.13372025998513237 1107 8 0.1759165990783771 1107 9 0.17970676288945953 1107 10-11 0.24186832925484225 1107 12-13 0.3156364952806072 1107 14-15 0.37904853793972393 1107 16-17 0.32380058831750347 1107 18-19 0.2433171340754754 1107 20-21 0.16776124125919267 1107 22-23 0.37682586860803724 1107 24-25 0.16890324317783012 1107 26-27 0.3874807980311914 1107 28-29 0.30121661264561084 1107 30-31 0.31518111179129704 1107 32-33 0.35122916173265395 1107 34-35 0.3136579146159164 1107 36-37 0.31134905315570194 1107 38-39 0.4527523004047538 1107 40-41 0.5471759192995407 1107 42-43 0.4702495413148142 1107 44-45 0.43962496115569394 1107 46-47 0.6417425533805456 1107 48-49 0.7480169684417888 1107 50-51 0.7250770403568936 1107 52-53 0.7007323663392597 1107 54-55 0.6955261007710405 1107 56-57 0.5314440499532722 1107 58-59 0.6527335931808196 1107 60-61 0.6811734213277489 1107 62-63 0.7140654088145055 1107 64-65 0.8964352493798486 1107 66-67 0.8532088607103177 1107 68-69 0.8842304011580566 1107 70-71 0.9267395570951678 1107 72-73 0.8295191341237427 1107 74-75 0.7697282357437061 1107 76-77 0.8082927358998617 1107 78-79 0.9903554074119434 1107 80-81 1.0481928852817894 1107 82-83 0.9760525348835962 1107 84-85 1.24154716828102 1107 86-87 1.2810804337443678 1107 88-89 1.1321829178731484 1107 90-91 1.2260972075768954 1107 92-93 1.3150144909426231 1107 94-95 1.487937500764172 1107 96-97 1.3085809469647707 1107 98-99 1.4318367577598892 1107 100-101 1.8213672989403094 1108 1 0.0779022512587666 1108 2 0.02738809525805408 1108 3 0.03361517417804194 1108 4 0.03178844748524767 1108 5 0.03403970029075509 1108 6 0.13281341266513635 1108 7 0.14875479987875195 1108 8 0.15767952334295643 1108 9 0.15240724276394957 1108 10-11 0.097943773643145 1108 12-13 0.16673182086380933 1108 14-15 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94-95 -3.6281722309205655 2316 96-97 -3.8083691055756823 2316 98-99 -3.655637030289977 2316 100-101 -4.239681253656549 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5538.0 3 1001.0 4 934.0 5 1020.0 6 1165.0 7 1351.0 8 1702.0 9 2137.0 10 2686.0 11 3597.0 12 5129.0 13 6944.0 14 7994.0 15 10519.0 16 13663.0 17 18335.0 18 15212.0 19 9640.0 20 9104.0 21 9581.0 22 10385.0 23 11548.0 24 13033.0 25 14730.0 26 17294.0 27 20426.0 28 24726.0 29 30299.0 30 36969.0 31 47048.0 32 60016.0 33 79012.0 34 108518.0 35 153616.0 36 227002.0 37 325324.0 38 279458.0 39 37429.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11579633956747 19.897108627668256 15.328578514122086 21.65851651864219 2 14.490130076040275 21.062711617442588 41.027852621648385 23.419305684868753 3 19.02799489295707 25.941456692621706 30.29868051593975 24.731867898481468 4 11.677464326971224 17.076438544097115 39.076992987952266 32.169104140979385 5 12.586732558096458 38.886501518244245 34.397771348341664 14.128994575317632 6 29.867346051016764 37.72644002559415 18.00136115452559 14.404852768863496 7 27.33804028217015 33.97467773400145 21.067873593498785 17.619408390329614 8 25.953025260583445 34.778804851473296 20.087599473341946 19.180570414601313 9 26.302577052137632 17.07704997831429 21.09775967790396 35.52261329164412 10-11 24.37261465837743 27.537293861746605 27.486015766008983 20.604075713866994 12-13 25.78666372150448 25.135447638944502 28.04070000252971 21.037188637021305 14-15 22.419252144556427 26.15053971748122 27.474568867654465 23.955639270307888 16-17 20.791949704160263 29.890363460245183 27.728666530932067 21.589020304662483 18-19 20.688871223625878 28.294692369932818 30.60022512413755 20.416211282303756 20-21 21.972319993436106 26.869822414025506 30.44800722028119 20.709850372257193 22-23 21.291918422945614 27.07274023934172 30.471280351103612 21.164060986609055 24-25 21.39069843043069 27.13622457650251 30.64625024712952 20.826826745937282 26-27 21.091981321209804 27.212832106792344 30.832156141855172 20.863030430142683 28-29 20.517813928164347 27.890168908028336 30.7925304739327 20.79948668987462 30-31 20.90386834896619 27.2438056036407 30.698284699895755 21.15404134749736 32-33 20.76233618237134 27.476506696462998 30.550827039556854 21.210330081608806 34-35 21.417466939850165 27.233147059703715 30.39593198878594 20.953454011660174 36-37 21.29973720250213 27.000684754043753 30.695148733513467 21.004429309940654 38-39 20.869139832804816 27.544177671179508 30.69339149354473 20.893291002470946 40-41 21.021445513344457 27.554600776640335 30.512681321460626 20.91127238855458 42-43 20.869336690990554 27.644502077920556 29.93815745680908 21.548003774279806 44-45 21.106302067765252 27.477066045531984 29.792902666381643 21.623729220321124 46-47 21.42027101521657 27.941861939381113 29.40814637425297 21.229720671149348 48-49 21.39386271709692 27.986248201817265 29.227538025221296 21.39235105586452 50-51 21.05664871135134 28.175406771190048 29.411793742171543 21.35615077528707 52-53 21.481399655088037 28.550150933696862 28.63624007082143 21.33220934039367 54-55 21.067239816603536 28.077724462301735 28.973173547324667 21.88186217377006 56-57 21.483895621557053 28.421773688383954 28.551372367353345 21.542958322705648 58-59 21.90868215916192 27.99175759609507 28.72112583267587 21.378434412067147 60-61 21.572846027729835 28.077231013302377 29.374376239040622 20.975546719927166 62-63 21.283450961619724 28.24183084351233 29.282009935431418 21.192708259436532 64-65 21.135587825642833 28.524073235634827 28.982875415284443 21.357463523437893 66-67 21.21374082292584 28.43805079792935 28.502847379531865 21.845360999612947 68-69 21.012908303429015 28.352223734057368 28.927077460922902 21.707790501590715 70-71 21.419318423658083 28.3013025146583 28.768116612536588 21.51126244914703 72-73 21.338878452038415 27.995488319689006 29.1116264595184 21.554006768754178 74-75 20.93775504601701 28.884302644428146 28.61734291324472 21.560599396310128 76-77 21.182211460606577 28.24916910216766 28.68970303595802 21.878916401267745 78-79 21.269083475012277 28.320777576808904 28.66580384396152 21.744335104217303 80-81 21.31815810975747 28.581765981223544 28.48395716399463 21.61611874502435 82-83 21.23413595894541 28.3529957126339 28.89026581916574 21.522602509254956 84-85 21.200656154906344 28.431374665500087 28.593100381276702 21.774868798316867 86-87 21.1216554084165 28.504637301153334 28.56866175627469 21.80504553415547 88-89 21.21273616619685 28.230704227089642 28.411865211786257 22.144694394927246 90-91 21.172678019673967 27.99928691583078 28.79283979601845 22.035195268476805 92-93 21.53340219100633 28.207086213240228 28.72694211175113 21.532569484002313 94-95 21.15432714622536 28.042356271944907 28.707800628836743 22.09551595299299 96-97 20.78489134128883 28.105144797069453 29.002744146714704 22.107219714927012 98-99 20.853114189412594 28.296720243588286 28.406152380858384 22.444013186140737 100-101 20.80182492545456 27.817592570034478 29.049207547030164 22.331374957480804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2348.0 1 1340.5 2 396.5 3 471.0 4 422.5 5 426.0 6 752.0 7 1597.0 8 2614.0 9 3034.5 10 2719.0 11 2359.5 12 2450.0 13 2678.0 14 2816.5 15 2940.0 16 3045.5 17 3127.0 18 3305.5 19 3531.0 20 3856.0 21 4351.5 22 5087.0 23 6133.0 24 7149.0 25 8670.0 26 11145.0 27 13965.5 28 16060.0 29 18482.0 30 22183.5 31 25695.5 32 29323.5 33 33832.0 34 39073.5 35 43063.5 36 47239.0 37 51536.5 38 53472.5 39 54276.0 40 54602.0 41 55542.5 42 56538.5 43 58296.5 44 60543.0 45 61145.0 46 59879.5 47 63903.0 48 75659.0 49 73236.0 50 59466.5 51 51504.0 52 46656.5 53 43799.0 54 40966.0 55 36877.0 56 32466.0 57 28529.0 58 25421.0 59 22641.5 60 19367.5 61 15800.0 62 12245.0 63 8914.0 64 6425.5 65 4887.0 66 3527.5 67 2585.5 68 1805.5 69 1162.5 70 878.0 71 689.0 72 568.5 73 490.5 74 359.5 75 280.5 76 204.0 77 116.5 78 81.0 79 53.5 80 34.5 81 24.5 82 16.5 83 12.0 84 9.5 85 11.5 86 9.5 87 6.5 88 6.5 89 5.5 90 3.0 91 2.0 92 2.5 93 3.0 94 4.5 95 6.0 96 5.5 97 6.0 98 8.0 99 6.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31426771742751186 2 0.2724597667744396 3 0.26901168837006256 4 0.2741222331479786 5 0.27455324294852573 6 0.2099633456951049 7 0.19869551805222976 8 0.20362134434419704 9 0.1976487799651867 10-11 0.2166132111892607 12-13 0.20663841294802698 14-15 0.1998038289679224 16-17 0.20214359645660687 18-19 0.1976179935508619 20-21 0.19109127371400528 22-23 0.19376969176026249 24-25 0.18345624296145602 26-27 0.1849032044347214 28-29 0.19952675123899927 30-31 0.21024042342402804 32-33 0.20392920848744497 34-35 0.21233389959811416 36-37 0.18921330244019277 38-39 0.18801263228152576 40-41 0.18521106857796935 42-43 0.1921995846296979 44-45 0.19632496414922052 46-47 0.20081978064064065 48-49 0.20685391784830057 50-51 0.20953233589455778 52-53 0.22655722301616965 54-55 0.20577639334693273 56-57 0.18068546567222443 58-59 0.18250186411738736 60-61 0.17726817368217213 62-63 0.186319379493662 64-65 0.17948479551355742 66-67 0.17643694049540268 68-69 0.17308122133399997 70-71 0.18459534029147345 72-73 0.18271736901766092 74-75 0.18853600132504728 76-77 0.19121441937130446 78-79 0.18822813718179932 80-81 0.18924408885451757 82-83 0.18068546567222443 84-85 0.17422031866401738 86-87 0.17471290129321412 88-89 0.1775144649967705 90-91 0.18262500977468654 92-93 0.176991095953249 94-95 0.17520548392241084 96-97 0.17388166810644465 98-99 0.18453376746282388 100-101 0.17034123045909316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1624093.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.619067331995225 #Duplication Level Percentage of deduplicated Percentage of total 1 74.87923482369155 38.651962641324154 2 13.656692059863056 14.098914139407913 3 4.76388564167766 7.3772200109905315 4 2.2020841627128833 4.5467812266318655 5 1.1709471137260645 3.02215989528156 6 0.732575753474019 2.2688926286617517 7 0.4815577279994405 1.7400292550092031 8 0.3355315291870951 1.3855859677692788 9 0.24513927743950223 1.1388474781079692 >10 1.3612008036749332 12.91835625970483 >50 0.11503680939008412 4.092422351790479 >100 0.05170636126088925 4.637211804150679 >500 0.0022635346527991665 0.7694323390584848 >1k 0.0017870010416835525 1.9747451464071344 >5k 3.574002083367105E-4 1.377438855704204 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9054 0.5574803905933958 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6887 0.42405207090973235 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6458 0.39763732741905794 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4992 0.30737156061875764 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4027 0.2479537809719025 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3020 0.18594994252176447 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2508 0.15442465425317395 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2234 0.13755369920318603 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1950 0.12006701586670221 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1948 0.11994387020940304 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1925 0.11852769515046245 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1702 0.10479695436160366 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.157282864959088E-5 0.0 3 0.0 0.0 0.0 6.157282864959088E-5 0.0 4 0.0 0.0 0.0 6.157282864959088E-5 0.0 5 0.0 0.0 0.0 6.157282864959088E-5 0.0 6 0.0 0.0 0.0 6.157282864959088E-5 0.0 7 0.0 0.0 0.0 6.157282864959088E-5 0.0 8 0.0 0.0 0.0 6.157282864959088E-5 0.0 9 0.0 0.0 0.0 6.157282864959088E-5 0.0 10-11 0.0 0.0 0.0 1.2314565729918176E-4 0.0 12-13 0.0 0.0 0.0 1.2314565729918176E-4 0.0 14-15 0.0 0.0 0.0 1.2314565729918176E-4 0.0 16-17 0.0 0.0 0.0 1.8471848594877262E-4 0.0 18-19 0.0 0.0 0.0 2.462913145983635E-4 0.0 20-21 0.0 0.0 0.0 2.7707772892315895E-4 0.0 22-23 0.0 0.0 0.0 4.002233862223407E-4 0.0 24-25 0.0 0.0 0.0 6.773011151454996E-4 0.0 26-27 0.0 0.0 0.0 8.620196010942723E-4 0.0 28-29 0.0 0.0 0.0 0.0014777478875901812 0.0 30-31 0.0 0.0 0.0 0.0031094278468043394 0.0 32-33 0.0 0.0 0.0 0.0054491953354887926 0.0 34-35 0.0 0.0 0.0 0.007265593780651724 0.0 36-37 0.0 0.0 0.0 0.008620196010942723 0.0 38-39 0.0 0.0 0.0 0.010436594456105654 0.0 40-41 0.0 0.0 0.0 0.013145798916687653 0.0 42-43 0.0 0.0 0.0 0.01579343054862006 0.0 44-45 0.0 0.0 0.0 0.019549373096245104 0.0 46-47 0.0 0.0 0.0 0.02465991787416115 0.0 48-49 0.0 0.0 0.0 0.03112506488236819 0.0 50-51 0.0 0.0 0.0 0.04060728049440518 0.0 52-53 0.0 0.0 0.0 0.06437439235314726 0.0 54-55 0.0 0.0 0.0 0.09371384520467732 0.0 56-57 0.0 0.0 0.0 0.13244315442527 0.0 58-59 0.0 0.0 0.0 0.1803160287003269 0.0 60-61 0.0 0.0 0.0 0.2504474805322109 0.0 62-63 0.0 0.0 0.0 0.3014605690683969 0.0 64-65 0.0 0.0 0.0 0.35484421150759227 0.0 66-67 0.0 0.0 0.0 0.4580094859099818 0.0 68-69 0.0 0.0 0.0 0.5880820864322425 0.0 70-71 0.0 0.0 0.0 0.7026691205491311 0.0 72-73 0.0 0.0 0.0 0.8257532050196632 0.0 74-75 0.0 0.0 0.0 1.024633441557842 0.0 76-77 0.0 0.0 0.0 1.1738859782044502 0.0 78-79 0.0 0.0 0.0 1.3239081752091784 0.0 80-81 0.0 0.0 0.0 1.512074739562328 0.0 82-83 0.0 0.0 0.0 1.78564281725246 0.0 84-85 0.0 0.0 0.0 2.0649679544213293 0.0 86-87 0.0 0.0 0.0 2.3987542585307615 0.0 88-89 0.0 0.0 0.0 2.8162180367749876 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9060 0.0 26.347515 1 TACATGG 9330 0.0 24.808296 2 ACATGGG 9210 0.0 24.253502 3 GAGTACT 7355 0.0 22.800303 12-13 GTGTAGC 805 0.0 22.446669 1 GTATCAA 16965 0.0 21.498377 1 AGTACTT 7790 0.0 21.43564 12-13 GTACTTT 7835 0.0 21.309568 14-15 GTATAGG 565 0.0 21.040483 1 ACAGCGT 160 4.9860413E-5 20.783882 8 CATGGGA 6050 0.0 20.506985 4 CATGGGG 3190 0.0 19.818811 4 AGAGTAC 13485 0.0 19.622433 10-11 GTACCCG 125 0.0059945555 19.020596 1 TCAACGC 19170 0.0 18.895115 4 ATCAACG 19195 0.0 18.867012 3 CAACGCA 19300 0.0 18.74379 5 AACGCAG 19635 0.0 18.460447 6 TATCAAC 19660 0.0 18.421335 2 TACTTTT 9035 0.0 18.40044 14-15 >>END_MODULE