FastQCFastQC Report
Thu 26 May 2016
SRR937230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624093
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115640.7120281905038689No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95200.5861733287441052No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75150.4627198073016755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69370.4271307123422119No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34430.21199524904054137No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30810.18970588506938948No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23780.14642018652872713No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22370.13773841768913478No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22080.13595280565829668No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT19390.11938971475155673No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19320.11895870495100959No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC18410.11335557754389682No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA17050.10498167284755246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG88650.022.9466861
TAGCGCG1252.2538564E-422.8038884
GAGTACT94800.022.62369512-13
AGTACTT99950.022.47980312-13
TACATGG92300.021.7209282
ACATGGG91550.021.328053
GTACTTT100500.021.3149614-15
GTATCAA192650.020.994981
AGAGTAC156650.020.25630610-11
TACTTTT112000.019.88972914-15
CATGGGA61900.019.2641894
ACTTTTT114000.018.89498116-17
GGTATCA151050.018.8478151
AGCGCGC1559.6178247E-418.3896665
TCAACGC221800.018.1207624
CAACGCA221800.018.0987875
ATCAACG222650.018.0302473
AACGCAG225350.017.8103796
TATCAAC225500.017.7602332
CGCGCGT1350.00939946217.5934267
Produced by FastQC (version 0.11.5)