##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937230_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1624093 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.156965149163256 33.0 31.0 34.0 31.0 34.0 2 32.37485168644899 34.0 31.0 34.0 31.0 34.0 3 32.45073096183531 34.0 31.0 34.0 31.0 34.0 4 36.07682811267581 37.0 35.0 37.0 35.0 37.0 5 35.94434924600993 37.0 35.0 37.0 35.0 37.0 6 36.04826201455212 37.0 35.0 37.0 35.0 37.0 7 36.012716636301 37.0 35.0 37.0 35.0 37.0 8 35.99184898894337 37.0 35.0 37.0 35.0 37.0 9 37.685288342477925 39.0 37.0 39.0 35.0 39.0 10-11 37.60353286418943 39.0 37.0 39.0 35.0 39.0 12-13 37.51911682397498 39.0 37.0 39.0 35.0 39.0 14-15 38.69217064540023 40.0 38.0 41.0 34.5 41.0 16-17 38.68628674589448 40.0 38.0 41.0 34.5 41.0 18-19 38.74099266482892 40.0 38.0 41.0 35.0 41.0 20-21 38.67323515340563 40.0 38.0 41.0 34.0 41.0 22-23 38.544208367377976 40.0 38.0 41.0 34.0 41.0 24-25 38.39466212833871 40.0 38.0 41.0 34.0 41.0 26-27 38.175505959326216 40.0 38.0 41.0 34.0 41.0 28-29 38.17820469640593 40.0 38.0 41.0 34.0 41.0 30-31 38.26424933793816 40.0 38.0 41.0 34.0 41.0 32-33 38.182216166192454 40.0 37.0 41.0 34.0 41.0 34-35 38.04559283243016 40.0 37.0 41.0 33.5 41.0 36-37 37.87770651064933 40.0 37.0 41.0 33.0 41.0 38-39 37.727717255107926 40.0 37.0 41.0 33.0 41.0 40-41 37.57365064685335 40.0 37.0 41.0 33.0 41.0 42-43 37.34483924258032 40.0 37.0 41.0 33.0 41.0 44-45 37.00608678197615 40.0 36.0 41.0 31.5 41.0 46-47 36.59569310378161 40.0 36.0 41.0 30.5 41.0 48-49 36.219300865159816 39.0 36.0 41.0 30.0 41.0 50-51 35.995341091920224 39.0 36.0 41.0 29.0 41.0 52-53 35.78323778256541 39.0 35.0 40.5 28.5 41.0 54-55 35.6001691405603 39.0 35.0 40.0 28.0 41.0 56-57 35.3677815863993 39.0 35.0 40.0 27.5 41.0 58-59 35.12666331299994 38.0 35.0 40.0 27.0 41.0 60-61 34.78972663511264 38.0 34.0 40.0 26.0 41.0 62-63 34.4497100843363 38.0 34.0 40.0 26.0 41.0 64-65 34.12473331268591 37.0 34.0 40.0 26.0 41.0 66-67 33.74205541185141 37.0 33.5 39.0 25.0 41.0 68-69 33.30871354041918 36.0 33.0 39.0 23.5 40.0 70-71 32.9204014178991 36.0 32.5 39.0 22.5 40.0 72-73 32.48930326034285 35.0 32.0 38.0 22.0 40.0 74-75 32.056369308900415 35.0 32.0 37.0 20.5 39.0 76-77 30.737410973386375 34.0 30.0 36.0 20.0 38.5 78-79 31.360616048465204 34.5 31.0 36.0 20.0 39.0 80-81 31.169446269394673 35.0 31.0 36.0 19.5 38.0 82-83 30.826149734036164 34.0 31.0 35.5 18.0 37.0 84-85 30.477213435437502 34.0 31.0 35.0 16.0 37.0 86-87 30.15149347974531 34.0 31.0 35.0 10.0 36.0 88-89 29.7770324728941 34.0 30.0 35.0 7.0 36.0 90-91 29.316278378147064 34.0 29.5 35.0 2.0 35.5 92-93 28.915000249369957 34.0 29.0 35.0 2.0 35.0 94-95 28.51468358031221 34.0 29.0 35.0 2.0 35.0 96-97 28.078851087961098 34.0 29.0 35.0 2.0 35.0 98-99 27.446795226628034 33.0 27.5 35.0 2.0 35.0 100-101 25.813446028029183 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03305127598746793 1101 2 0.08993752123611642 1101 3 0.09570323366547484 1101 4 -0.022587175199575427 1101 5 -0.03744049810463679 1101 6 -0.014053087619203097 1101 7 0.03912190719118769 1101 8 -0.016921721961260516 1101 9 -0.01902803252013996 1101 10-11 0.039589944403147115 1101 12-13 0.11406008851426463 1101 14-15 0.0718192814457268 1101 16-17 0.1428774819482328 1101 18-19 0.1623485872317616 1101 20-21 -0.02145600488483268 1101 22-23 0.0025191862333002746 1101 24-25 0.0315447437975962 1101 26-27 0.0023593053444841416 1101 28-29 -0.021077460520182 1101 30-31 0.05022550890994637 1101 32-33 0.08346149891885801 1101 34-35 0.16887093896251315 1101 36-37 0.0600810137531127 1101 38-39 0.07364942243635397 1101 40-41 0.14628418605974502 1101 42-43 0.03766431995165931 1101 44-45 0.06796334542923432 1101 46-47 -0.038247929819853255 1101 48-49 -0.0071654297435586045 1101 50-51 -0.012274424599098666 1101 52-53 -0.008301689724326877 1101 54-55 0.07585874200197651 1101 56-57 0.01234136071926173 1101 58-59 0.03156482668477878 1101 60-61 -0.05230495276165925 1101 62-63 -0.16675850552854854 1101 64-65 -0.024306541769377077 1101 66-67 0.001962140891038189 1101 68-69 -0.11171338318425228 1101 70-71 -0.18785105485371645 1101 72-73 -0.23735743101234874 1101 74-75 -0.19148261507945108 1101 76-77 -0.15479488934842678 1101 78-79 -0.05456155955316433 1101 80-81 -0.25771669890942306 1101 82-83 -0.09589612377385137 1101 84-85 -0.08469040528794736 1101 86-87 -0.28107604007447407 1101 88-89 -0.22829821113102966 1101 90-91 -0.02340497323967128 1101 92-93 0.008137503297643178 1101 94-95 -0.10064367017500331 1101 96-97 -0.12196172558961038 1101 98-99 0.052955313904682555 1101 100-101 0.2848589065133673 1102 1 0.17842742848720405 1102 2 0.13435598179056996 1102 3 0.10516765144074469 1102 4 0.10286789979678446 1102 5 0.042539057428072624 1102 6 0.10449284247556534 1102 7 0.1117425966494352 1102 8 0.12476814460718089 1102 9 0.14250153343468241 1102 10-11 0.19345897417611724 1102 12-13 0.14129877195910012 1102 14-15 0.20848426344259963 1102 16-17 0.24853069124418425 1102 18-19 0.0756142196825067 1102 20-21 0.22488640567776486 1102 22-23 0.11977363133448193 1102 24-25 0.09253521541948118 1102 26-27 0.13394229531927238 1102 28-29 0.11044256849040579 1102 30-31 0.1609531434907936 1102 32-33 0.17920742216072938 1102 34-35 0.2581373080993643 1102 36-37 0.2110684015726676 1102 38-39 0.12287718693664829 1102 40-41 0.18465254053710822 1102 42-43 0.30068724573767014 1102 44-45 0.3764763530787789 1102 46-47 0.49916330378673734 1102 48-49 0.37095484996872585 1102 50-51 0.38493202168991303 1102 52-53 0.3830281143685639 1102 54-55 0.4096802012686567 1102 56-57 0.46777127988367084 1102 58-59 0.4281947732607492 1102 60-61 0.4059710589682055 1102 62-63 0.46636210255158517 1102 64-65 0.4845803769124686 1102 66-67 0.43377213298165174 1102 68-69 0.37407014531379446 1102 70-71 0.2857253277890379 1102 72-73 0.4347251634933329 1102 74-75 0.33891853620675505 1102 76-77 0.20656792285086567 1102 78-79 0.18351740291867458 1102 80-81 0.238493222367957 1102 82-83 0.27710133208922727 1102 84-85 0.1539146595718357 1102 86-87 0.30914302410573313 1102 88-89 0.30784837798371356 1102 90-91 0.19656393175351994 1102 92-93 0.06347819207060468 1102 94-95 -0.04081090934386111 1102 96-97 0.21030041474053007 1102 98-99 0.11546270218109811 1102 100-101 0.09956750147020799 1103 1 0.0955371810963257 1103 2 0.05903284606914383 1103 3 0.1244915067160477 1103 4 0.0901893015113302 1103 5 0.024221397983488657 1103 6 0.057028532642746654 1103 7 0.017012444098114088 1103 8 0.03698581555283198 1103 9 -0.04830203720398174 1103 10-11 0.07773288828379776 1103 12-13 0.010920180456324147 1103 14-15 0.013887110398798086 1103 16-17 0.029814770524865253 1103 18-19 0.002599259449652891 1103 20-21 0.011638799521449528 1103 22-23 0.09490360812591803 1103 24-25 -0.008428367486992272 1103 26-27 0.03801721149419279 1103 28-29 -0.11082939043604512 1103 30-31 0.02816375160228546 1103 32-33 0.05584418605564423 1103 34-35 0.16269954508620543 1103 36-37 0.24383936226555392 1103 38-39 0.18598353640981458 1103 40-41 0.23945792185643455 1103 42-43 0.2406090679783759 1103 44-45 0.39632660330068603 1103 46-47 0.4306175398652883 1103 48-49 0.18927398390896855 1103 50-51 0.3191376666490626 1103 52-53 0.3366111387950568 1103 54-55 0.27608426026027644 1103 56-57 0.21869490395805258 1103 58-59 0.16030973083951494 1103 60-61 0.22575038594941788 1103 62-63 0.3592324737411161 1103 64-65 0.4984751392090274 1103 66-67 0.4821295448289291 1103 68-69 0.42243094850106644 1103 70-71 0.477570924502686 1103 72-73 0.4749766829611062 1103 74-75 0.4923971663413127 1103 76-77 0.23214673611778736 1103 78-79 0.48121777950486333 1103 80-81 0.4600298532633573 1103 82-83 0.4013326037827447 1103 84-85 0.4320369778328761 1103 86-87 0.3823422962946843 1103 88-89 0.4303235253595581 1103 90-91 0.5689837855425317 1103 92-93 0.558835742520472 1103 94-95 0.550444889110274 1103 96-97 0.5480486829453888 1103 98-99 0.5850786550444198 1103 100-101 1.0030955272090054 1104 1 0.031123982044967136 1104 2 0.07016364632915639 1104 3 -0.005397214963487329 1104 4 0.09308063818686918 1104 5 -0.053208900945286075 1104 6 0.010868955553789306 1104 7 -0.05752444040573579 1104 8 -0.022852476180986514 1104 9 -0.05911494180857346 1104 10-11 0.058753241323096006 1104 12-13 -0.0282951129664184 1104 14-15 -0.030713561011069146 1104 16-17 0.03727967882191052 1104 18-19 -0.010430900566731793 1104 20-21 -0.029285887999883187 1104 22-23 0.0072368316353603745 1104 24-25 -0.04347688293505314 1104 26-27 -0.039805782067752205 1104 28-29 -0.1250658323792777 1104 30-31 -0.08513369603101495 1104 32-33 -0.027994634020167553 1104 34-35 -0.027407251232020258 1104 36-37 -0.05820514312620162 1104 38-39 -0.15121025527503917 1104 40-41 -0.17696505448987665 1104 42-43 -0.05111392053760966 1104 44-45 0.1198675037972734 1104 46-47 -0.01873256981019722 1104 48-49 -0.07117754071384752 1104 50-51 0.03781811769876953 1104 52-53 0.16104769768589478 1104 54-55 0.12395961310664916 1104 56-57 0.14049836073937882 1104 58-59 0.1755495773235154 1104 60-61 -0.12951641191227736 1104 62-63 -0.026625325091188756 1104 64-65 -0.027236712570271493 1104 66-67 0.04095463691775336 1104 68-69 -0.04726270219364892 1104 70-71 -0.06320060320707199 1104 72-73 -0.2020052673331989 1104 74-75 -0.17310492179681702 1104 76-77 -0.19003338694433936 1104 78-79 -0.1700120997185337 1104 80-81 -0.19597187423684304 1104 82-83 -0.2680036740182814 1104 84-85 -0.3665712269026109 1104 86-87 -0.13697661745793965 1104 88-89 0.021639423407361136 1104 90-91 0.17443134760044998 1104 92-93 -0.03824932350743637 1104 94-95 -0.22999265522741297 1104 96-97 -0.2715513206164637 1104 98-99 -0.028911425186386452 1104 100-101 0.06434268143440391 1105 1 0.0010420159937538642 1105 2 0.04508795206672289 1105 3 0.005750855650909159 1105 4 0.06043989089245372 1105 5 -0.13523919312709864 1105 6 -0.014829845975086187 1105 7 0.03566116080983761 1105 8 -0.033848238388053176 1105 9 -0.03819198905222976 1105 10-11 0.06586091673187155 1105 12-13 0.06617560928609123 1105 14-15 0.06500443137712608 1105 16-17 0.11085338598743277 1105 18-19 0.145331439204071 1105 20-21 0.09262553124001727 1105 22-23 0.14608434256913938 1105 24-25 0.1340533442517824 1105 26-27 0.12934989552219633 1105 28-29 -0.015121480744106464 1105 30-31 0.023789284472606198 1105 32-33 -0.00596133789431974 1105 34-35 0.13145991804233859 1105 36-37 0.13379212075128066 1105 38-39 0.13310580909983827 1105 40-41 0.10329146302814962 1105 42-43 -0.10920675990109174 1105 44-45 0.1586492959152963 1105 46-47 0.12459326070040078 1105 48-49 0.20261235390984922 1105 50-51 0.130170395885699 1105 52-53 0.22799252072312726 1105 54-55 0.1316585768967542 1105 56-57 0.03058401838446656 1105 58-59 -0.028784215146778536 1105 60-61 0.034081064412802675 1105 62-63 0.10329193675953974 1105 64-65 0.14628343029199442 1105 66-67 0.019779696687493242 1105 68-69 0.06752581079675224 1105 70-71 -0.012320999534658483 1105 72-73 0.06837880018195364 1105 74-75 0.0870663392052009 1105 76-77 0.15606650746521566 1105 78-79 0.14890300598802497 1105 80-81 0.20220724105338306 1105 82-83 0.17502875922765782 1105 84-85 0.22086103732623386 1105 86-87 0.31025696422355153 1105 88-89 0.2831696976439275 1105 90-91 0.2134621111843238 1105 92-93 0.2195683956002732 1105 94-95 0.19272847169796847 1105 96-97 0.14428938710792139 1105 98-99 0.169542659559216 1105 100-101 0.39439711133197264 1106 1 -0.24729550797377087 1106 2 -0.08000574388787385 1106 3 -0.14777225559314644 1106 4 -0.08808727082015366 1106 5 -0.1407617194981512 1106 6 -0.0974469806516538 1106 7 -0.19712466640478254 1106 8 -0.003585838745863157 1106 9 -0.08523572041457328 1106 10-11 -0.1334801136066659 1106 12-13 -0.4520487914500393 1106 14-15 -0.21664250219678394 1106 16-17 -0.14640345130860766 1106 18-19 -0.221909078351203 1106 20-21 -0.4186128863790657 1106 22-23 -0.307833334962595 1106 24-25 -0.13675384739256202 1106 26-27 -0.0033078075515433625 1106 28-29 -0.1543693582699106 1106 30-31 -0.08063443881747645 1106 32-33 -0.005507073784656313 1106 34-35 -0.08904325750589237 1106 36-37 -0.07175745121788424 1106 38-39 -0.09984455346726406 1106 40-41 -0.1625543426583036 1106 42-43 -0.3724459624477774 1106 44-45 -0.10520552051071519 1106 46-47 -0.07078761235953834 1106 48-49 -0.039603117000524435 1106 50-51 0.18575260415082084 1106 52-53 0.20501825672815244 1106 54-55 0.2701565682275131 1106 56-57 0.21217561838484045 1106 58-59 0.125212432982984 1106 60-61 0.23315753209841716 1106 62-63 0.12380693082002381 1106 64-65 0.03755040984660951 1106 66-67 0.1972321096549834 1106 68-69 0.21872902135454098 1106 70-71 0.1635226941103909 1106 72-73 0.28028456554530834 1106 74-75 0.2451209420060323 1106 76-77 0.15551938361644702 1106 78-79 0.2548857535086064 1106 80-81 0.2810191970553859 1106 82-83 0.22593405408042244 1106 84-85 0.21288401939490953 1106 86-87 0.46066871673052745 1106 88-89 0.22374169452332993 1106 90-91 0.27932021322045486 1106 92-93 0.2816920389003741 1106 94-95 0.2054983582561789 1106 96-97 0.3085125524750332 1106 98-99 0.1845387906688849 1106 100-101 0.1939747349063019 1107 1 0.004057273244860937 1107 2 0.07689422209715246 1107 3 -7.484380920885769E-4 1107 4 0.05508289913345976 1107 5 -0.007097553857356331 1107 6 0.02691188816223189 1107 7 0.046849482215755245 1107 8 0.0361360553456791 1107 9 -0.009318831820948503 1107 10-11 0.0728096888575962 1107 12-13 0.10411723607975887 1107 14-15 0.15411942681594581 1107 16-17 0.08463463716478259 1107 18-19 0.15707052989586145 1107 20-21 0.12057070321529295 1107 22-23 0.07101975417769779 1107 24-25 0.04702608402865849 1107 26-27 0.06588697571309154 1107 28-29 -0.08632250057056723 1107 30-31 0.07736383426851745 1107 32-33 0.061588577212781104 1107 34-35 0.031597896016506866 1107 36-37 0.0030691075198490125 1107 38-39 0.09751456359848731 1107 40-41 0.0012038204565740784 1107 42-43 0.10080649438415179 1107 44-45 0.18610607897797848 1107 46-47 0.2305525009939302 1107 48-49 0.030548137947761234 1107 50-51 0.019061686659149757 1107 52-53 0.17786371451672522 1107 54-55 0.1419689408707967 1107 56-57 0.282286508473085 1107 58-59 0.21181595815948384 1107 60-61 0.28063346794094457 1107 62-63 0.3001847765426504 1107 64-65 0.1833642388157486 1107 66-67 0.18758490810446204 1107 68-69 0.23495457827792876 1107 70-71 0.19576448504657407 1107 72-73 0.22360448434333335 1107 74-75 0.2440939514759819 1107 76-77 0.2559345513587665 1107 78-79 0.44391646717847877 1107 80-81 0.5225929926349728 1107 82-83 0.4233236442223358 1107 84-85 0.27482222191099837 1107 86-87 0.5467985530948916 1107 88-89 0.6416642986534988 1107 90-91 0.642822051496811 1107 92-93 0.6507548147136184 1107 94-95 0.4380036216424763 1107 96-97 0.5631045738259708 1107 98-99 0.5929097606444458 1107 100-101 0.974164778411005 1108 1 -0.04633758317667258 1108 2 -0.02442547739654799 1108 3 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-0.6207117089582006 2316 98-99 -0.7387722237155607 2316 100-101 -0.8691576894143758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 13.0 5 34.0 6 70.0 7 141.0 8 380.0 9 729.0 10 1325.0 11 2207.0 12 3497.0 13 5147.0 14 6808.0 15 9636.0 16 13682.0 17 20041.0 18 17051.0 19 8926.0 20 7184.0 21 6940.0 22 7360.0 23 8007.0 24 9271.0 25 10960.0 26 13551.0 27 16550.0 28 20622.0 29 26531.0 30 33501.0 31 42590.0 32 55769.0 33 73607.0 34 102093.0 35 147874.0 36 231003.0 37 366928.0 38 322593.0 39 31459.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93819138752444 20.34550434098041 15.888345573333432 20.827958698161723 2 15.32843254665835 21.772152210495335 40.11580617612415 22.783609066722164 3 19.457752727214515 26.066241280517804 30.820771963181908 23.655234029085772 4 12.365432500129304 17.868630594818523 38.69546799072959 31.070468914322586 5 12.573280915925237 39.02547466327727 34.26449361039678 14.136750810400716 6 29.901243340128918 38.22693651164065 17.510265729856602 14.361554418373826 7 27.305271311433522 34.318847504422465 20.86265995851223 17.513221225631785 8 26.2467728141184 34.88870403357443 19.693453515285146 19.17106963702202 9 26.31936717909627 17.028827782645454 20.96203850395267 35.68976653430561 10-11 24.50062896644466 27.683882634799854 27.22568843040392 20.589799968351567 12-13 26.18087763330632 25.162776692668242 28.019820148226238 20.6365255257992 14-15 22.583004790981796 26.147117190949043 27.715253990996818 23.55462402707234 16-17 20.713684499594542 29.789519442544236 28.067235065972206 21.42956099188901 18-19 20.670491160296855 27.907761439769768 31.33148779041594 20.090259609517435 20-21 21.77532321117079 26.481488436930643 31.399956775874287 20.34323157602428 22-23 21.191854191417946 26.57427353240261 31.487471787778208 20.74640048840124 24-25 21.393751801015217 26.6287334891545 31.648239869366364 20.329274840463917 26-27 21.19686206850902 26.639136954928176 31.8929647763157 20.271036200247096 28-29 20.477570768186375 27.398498509130707 31.8024201803821 20.321510542300818 30-31 20.855293114005935 26.734721334116134 31.688398370406993 20.721587181470937 32-33 20.800101964604245 26.909142518316376 31.528089832294086 20.762665684785294 34-35 21.35545809260923 26.646626763368847 31.4621761192247 20.535739024797227 36-37 21.297478155445916 26.42773887391639 31.683331393784453 20.59145157685324 38-39 20.901520235947515 27.010325782438166 31.711666225393913 20.376487756220406 40-41 20.960136199138905 27.118036688181245 31.44993280863005 20.4718943040498 42-43 20.87926840119946 27.09900359967744 30.854571614983843 21.16715638413925 44-45 21.18081980577455 26.884129716789445 30.64780197180948 21.28724850562653 46-47 21.519765185860663 27.53426066118135 30.17370310690336 20.772271046054627 48-49 21.540268937800978 27.59974336445019 29.871411304648195 20.98857639310064 50-51 21.15913312846001 27.830425966985885 29.944005669626062 21.06643523492805 52-53 21.589978150861576 28.281380698070276 29.110428815125257 21.018212335942895 54-55 21.196784913179233 27.750750726713306 29.426732336140848 21.625732023966606 56-57 21.578320945906423 27.978847270445716 29.093007604860066 21.34982417878779 58-59 22.135185608213323 27.607501540860035 29.14485192658302 21.11246092434362 60-61 21.63915490061222 27.738436160983394 29.918514518565132 20.70389441983926 62-63 21.378978913153375 27.937531902421842 29.769723778133393 20.913765406291386 64-65 21.255340673224993 28.19767710346637 29.448744622383078 21.09823760092556 66-67 21.362138744517708 28.011203791901078 29.0161955011197 21.610461962461507 68-69 21.315558899644294 27.8426173870585 29.355061563592727 21.48676214970448 70-71 21.652485418014855 27.94276559285706 29.230253439920006 21.174495549208082 72-73 21.56939288575223 27.582256681113705 29.597073566599942 21.251276866534123 74-75 21.180252608686818 28.515331326469607 28.955176827927957 21.349239236915622 76-77 21.280708678628628 27.892091154878447 29.18610572177825 21.641094444714682 78-79 21.440951965189186 27.90028034108884 29.124625252371633 21.53414244135034 80-81 21.42946863264604 28.211869640470095 28.99698477858103 21.361676948302836 82-83 21.45390218304528 28.02809829168372 29.222648205804564 21.295351319466445 84-85 21.217763525711597 28.13159599333043 29.07292197732641 21.57771850363157 86-87 21.183392822947948 28.18924162594137 28.890679289917507 21.73668626119317 88-89 21.189396173741283 28.018038375881183 28.718767952327852 22.07379749804968 90-91 21.26484221415893 27.678095024038495 29.097867401086543 21.959195360716027 92-93 21.49605908918641 27.77590147122616 29.1042136235044 21.623825816083027 94-95 21.152760340694776 27.710943892991352 29.115758765046092 22.020537001267783 96-97 20.825131319450303 27.711251757134598 29.43405950275015 22.02955742066495 98-99 20.92656891618388 27.930528728186705 28.771257589742294 22.371644765887122 100-101 20.476866981835602 27.901441660801122 29.121320334923954 22.500371022439314 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 482.0 1 343.0 2 315.0 3 473.5 4 407.5 5 376.5 6 731.0 7 1804.5 8 3125.0 9 3737.0 10 3294.0 11 2683.5 12 2746.0 13 2990.0 14 3107.5 15 3235.5 16 3315.5 17 3429.5 18 3727.5 19 3985.5 20 4300.5 21 4791.5 22 5551.0 23 6479.5 24 7462.0 25 9023.0 26 11332.5 27 13878.0 28 15997.0 29 18727.0 30 22341.5 31 25493.0 32 28997.5 33 33531.0 34 38701.5 35 42605.0 36 47134.5 37 51497.5 38 53519.0 39 54194.5 40 54246.0 41 55398.0 42 56510.5 43 58613.5 44 60843.5 45 61248.5 46 59771.5 47 64055.5 48 76845.0 49 73863.5 50 59239.5 51 51389.5 52 46289.0 53 43550.5 54 40692.5 55 36536.5 56 31997.5 57 27856.0 58 25107.0 59 22458.0 60 19134.0 61 15374.5 62 11690.5 63 8663.0 64 6320.5 65 4764.5 66 3409.5 67 2470.0 68 1740.5 69 1072.0 70 714.0 71 542.5 72 474.5 73 446.0 74 352.5 75 287.5 76 215.5 77 108.5 78 68.5 79 44.5 80 17.0 81 13.0 82 12.5 83 7.5 84 4.5 85 2.5 86 1.0 87 0.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04476344642825257 2 0.0 3 0.0 4 6.157282864959088E-5 5 0.012622429873166129 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005479981749813589 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011390973300174313 24-25 5.541554578463179E-4 26-27 0.0018163984451629307 28-29 0.0024936995603084306 30-31 4.0022338622234074E-4 32-33 0.0 34-35 0.0 36-37 2.462913145983635E-4 38-39 1.8471848594877262E-4 40-41 3.078641432479544E-5 42-43 0.0121606336582942 44-45 6.157282864959088E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 9.235924297438631E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.233690435215225E-4 84-85 6.157282864959088E-5 86-87 0.0 88-89 0.0 90-91 0.008343118282019564 92-93 0.0024321267316588396 94-95 0.0 96-97 0.0 98-99 0.001600893544889363 100-101 0.012437711387217358 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1624093.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.741398124234316 #Duplication Level Percentage of deduplicated Percentage of total 1 73.49030401690003 36.555104703756406 2 14.355164989602113 14.280919537737372 3 5.0370905884726564 7.516557850471564 4 2.3030327511853486 4.582242758794444 5 1.2898250322569342 3.207885022004777 6 0.7687718337405316 2.2943871509271276 7 0.5120355279412911 1.7828554134356185 8 0.356031568949465 1.4167606412728897 9 0.25588890853720464 1.1455444867612388 >10 1.449321430726908 13.298111652864279 >50 0.11792504874958046 4.02379258670869 >100 0.060020157202522774 5.251113478568121 >500 0.0024800787758009046 0.829058257644277 >1k 0.0016120512042705878 1.6237600351525625 >5k 3.7201181637013566E-4 1.4786239529981853 >10k+ 1.240039387900452E-4 0.7132824709023451 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11564 0.7120281905038689 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9520 0.5861733287441052 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7515 0.4627198073016755 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6937 0.4271307123422119 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3443 0.21199524904054137 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3081 0.18970588506938948 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2378 0.14642018652872713 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2237 0.13773841768913478 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2208 0.13595280565829668 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1939 0.11938971475155673 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1932 0.11895870495100959 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1841 0.11335557754389682 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1705 0.10498167284755246 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.078641432479544E-5 0.0 18-19 0.0 0.0 0.0 1.2314565729918176E-4 0.0 20-21 0.0 0.0 0.0 1.539320716239772E-4 0.0 22-23 0.0 0.0 0.0 2.1550490027356808E-4 0.0 24-25 0.0 0.0 0.0 4.617962148719316E-4 0.0 26-27 0.0 0.0 0.0 6.465147008207041E-4 0.0 28-29 0.0 0.0 0.0 0.0012314565729918178 0.0 30-31 0.0 0.0 0.0 0.002801563703556385 0.0 32-33 0.0 0.0 0.0 0.005110544777916043 0.0 34-35 0.0 0.0 0.0 0.006896156808754179 0.0 36-37 0.0 0.0 0.0 0.008219972624720382 0.0 38-39 0.0 0.0 0.0 0.009944011826908926 0.0 40-41 0.0 0.0 0.0 0.012714789116140516 0.0 42-43 0.0 0.0 0.0 0.015208488676448948 0.0 44-45 0.0 0.0 0.0 0.0189028583954244 0.0 46-47 0.0 0.0 0.0 0.02370553903009249 0.0 48-49 0.0 0.0 0.0 0.03023225886694912 0.0 50-51 0.0 0.0 0.0 0.039745260893310916 0.0 52-53 0.0 0.0 0.0 0.06369709123800177 0.0 54-55 0.0 0.0 0.0 0.09282103918925826 0.0 56-57 0.0 0.0 0.0 0.1317350668957997 0.0 58-59 0.0 0.0 0.0 0.17966951399950618 0.0 60-61 0.0 0.0 0.0 0.2505398397751853 0.0 62-63 0.0 0.0 0.0 0.3015221418970465 0.0 64-65 0.0 0.0 0.0 0.35561387186571214 0.0 66-67 0.0 0.0 0.0 0.45896386475405043 0.0 68-69 0.0 0.0 0.0 0.5890364652763111 0.0 70-71 0.0 0.0 0.0 0.7046702374802428 0.0 72-73 0.0 0.0 0.0 0.8285239823088949 0.0 74-75 0.0 0.0 0.0 1.0286048890057404 0.0 76-77 0.0 0.0 0.0 1.1787810180820926 0.0 78-79 0.0 0.0 0.0 1.3296036618592655 0.0 80-81 0.0 0.0 0.0 1.519432692585954 0.0 82-83 0.0 0.0 0.0 1.7957407611509932 0.0 84-85 0.0 0.0 0.0 2.0773440929798968 0.0 86-87 0.0 0.0 0.0 2.4141474656931594 0.0 88-89 0.0 0.0 0.0 2.835890555528532 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8865 0.0 22.946686 1 TAGCGCG 125 2.2538564E-4 22.803888 4 GAGTACT 9480 0.0 22.623695 12-13 AGTACTT 9995 0.0 22.479803 12-13 TACATGG 9230 0.0 21.720928 2 ACATGGG 9155 0.0 21.32805 3 GTACTTT 10050 0.0 21.31496 14-15 GTATCAA 19265 0.0 20.99498 1 AGAGTAC 15665 0.0 20.256306 10-11 TACTTTT 11200 0.0 19.889729 14-15 CATGGGA 6190 0.0 19.264189 4 ACTTTTT 11400 0.0 18.894981 16-17 GGTATCA 15105 0.0 18.847815 1 AGCGCGC 155 9.6178247E-4 18.389666 5 TCAACGC 22180 0.0 18.120762 4 CAACGCA 22180 0.0 18.098787 5 ATCAACG 22265 0.0 18.030247 3 AACGCAG 22535 0.0 17.810379 6 TATCAAC 22550 0.0 17.760233 2 CGCGCGT 135 0.009399462 17.593426 7 >>END_MODULE