FastQCFastQC Report
Thu 26 May 2016
SRR937229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795495
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97730.5443067232156035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73690.41041606910629097No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69400.3865229365718089No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54570.3039273292323287No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44370.2471184826468467No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32590.18150983433537826No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26010.1448625587929791No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25670.14296893057346305No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21680.12074664646796567No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21410.11924288288187937No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20220.11261518411357313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC459.6219394E-442.2263039
GTACATG95350.028.4749721
TACATGG97650.027.2579922
CCGGTCC1056.9034126E-527.145489
ACATGGG99550.025.8305743
GAGTACT78200.023.41850912-13
CATGGGA65450.022.4395834
AGTACTT84350.021.99265312-13
CATGGGG32800.021.4463714
GTACTTT85700.021.31242614-15
GTATCAA186550.021.0793931
AGAGTAC138800.020.86055610-11
GTGTACG1856.4964097E-620.5620371
GTCTACG1250.005996095519.0198821
TACTTTT97300.018.94245514-15
ATGGGAG27700.018.8757025
ACTTTTT99950.018.55930516-17
CATTCCG2854.271351E-818.3351049
CAACGCA213400.018.1977565
TCAACGC213700.018.1934364
Produced by FastQC (version 0.11.5)