##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937229_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1795495 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.666804418837145 31.0 31.0 34.0 30.0 34.0 2 31.752114040974774 33.0 31.0 34.0 30.0 34.0 3 31.796399321635537 33.0 31.0 34.0 30.0 34.0 4 35.44090125564259 37.0 35.0 37.0 33.0 37.0 5 35.43294133372691 37.0 35.0 37.0 33.0 37.0 6 35.51526180802509 37.0 35.0 37.0 33.0 37.0 7 35.455371359987076 37.0 35.0 37.0 33.0 37.0 8 35.45147327060226 37.0 35.0 37.0 33.0 37.0 9 37.054263030529185 39.0 37.0 39.0 33.0 39.0 10-11 36.95935577654073 39.0 37.0 39.0 33.0 39.0 12-13 36.85012183269795 39.0 37.0 39.0 33.0 39.0 14-15 37.96856159443496 40.0 38.0 41.0 33.0 41.0 16-17 37.92358012692878 40.0 38.0 41.0 33.0 41.0 18-19 37.918552822480706 40.0 38.0 41.0 33.0 41.0 20-21 37.85724939362126 40.0 38.0 41.0 33.0 41.0 22-23 37.73132534482134 40.0 37.0 41.0 32.5 41.0 24-25 37.575717002832086 40.0 37.0 41.0 32.0 41.0 26-27 37.41049766220458 39.5 37.0 41.0 32.0 41.0 28-29 37.23070239683207 39.0 36.5 41.0 31.5 41.0 30-31 37.22997975488653 39.0 37.0 41.0 32.0 41.0 32-33 37.070319048507514 39.0 36.0 41.0 31.0 41.0 34-35 36.858022439494405 39.0 36.0 41.0 31.0 41.0 36-37 36.85017084425186 39.0 36.0 41.0 31.0 41.0 38-39 36.81919136505532 39.0 36.0 41.0 31.0 41.0 40-41 36.66987543824962 39.0 35.5 41.0 30.5 41.0 42-43 36.42556370248873 39.0 35.0 41.0 30.0 41.0 44-45 36.171842026850534 39.0 35.0 41.0 30.0 41.0 46-47 35.814995029225926 39.0 35.0 41.0 28.5 41.0 48-49 35.48517706816226 39.0 35.0 40.0 27.0 41.0 50-51 34.56249000971877 38.0 34.0 39.5 25.0 40.5 52-53 34.712651664304275 38.0 34.5 39.5 26.0 40.5 54-55 35.08644357127143 39.0 35.0 40.0 26.0 41.0 56-57 34.8742745036884 38.5 34.5 40.0 25.5 41.0 58-59 34.647840846117646 38.0 34.0 40.0 25.0 41.0 60-61 34.36220039599108 38.0 34.0 40.0 23.5 41.0 62-63 34.070789392340274 38.0 34.0 40.0 23.0 41.0 64-65 33.72665532346233 37.0 33.0 40.0 22.0 41.0 66-67 33.33343896808401 36.5 33.0 39.5 21.5 41.0 68-69 32.84294581717019 36.0 32.0 39.0 20.0 40.5 70-71 32.414046265793 35.5 32.0 39.0 18.5 40.0 72-73 31.99933555927474 35.0 31.5 38.0 17.5 40.0 74-75 31.551196188237782 35.0 31.0 37.5 13.5 39.5 76-77 31.115351198416036 35.0 31.0 37.0 9.5 39.0 78-79 30.62498391808387 35.0 30.5 36.0 7.0 39.0 80-81 30.157442933564283 34.0 30.0 36.0 4.5 37.5 82-83 29.703673360271125 34.0 29.5 35.5 2.0 37.0 84-85 29.227780639879253 34.0 29.0 35.0 2.0 37.0 86-87 28.80291256728646 34.0 29.0 35.0 2.0 36.0 88-89 28.40399249232106 34.0 28.5 35.0 2.0 36.0 90-91 27.949142437043825 34.0 27.0 35.0 2.0 35.5 92-93 27.50372042250187 33.0 26.5 35.0 2.0 35.0 94-95 26.952162495579216 33.0 25.5 35.0 2.0 35.0 96-97 26.314878348310632 33.0 25.0 35.0 2.0 35.0 98-99 25.591861854251892 32.0 22.5 34.5 2.0 35.0 100-101 23.728269084569995 30.5 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07112630774790318 1101 2 0.12363264007624153 1101 3 0.22864369442032384 1101 4 0.18902801357293697 1101 5 0.15792987112278922 1101 6 -0.00631694236072633 1101 7 0.13615977688650105 1101 8 -0.016261236527789436 1101 9 0.013042767198541583 1101 10-11 0.005095846811961735 1101 12-13 0.12222539717733127 1101 14-15 0.21887085820301166 1101 16-17 0.23078422003248278 1101 18-19 0.3249875797003412 1101 20-21 0.4173133708693584 1101 22-23 0.21348522184581498 1101 24-25 0.37976914699951436 1101 26-27 0.3922689259784491 1101 28-29 0.2650785974115948 1101 30-31 0.1983288383173587 1101 32-33 0.08001782922929834 1101 34-35 0.07956880386333154 1101 36-37 0.03221024884241075 1101 38-39 0.022457449395282936 1101 40-41 -0.005595717350942664 1101 42-43 0.020983135333331404 1101 44-45 -0.1316933603172643 1101 46-47 0.0063851780179788875 1101 48-49 0.16036183574717455 1101 50-51 0.14251364613320305 1101 52-53 0.1474211110110275 1101 54-55 0.218969276919303 1101 56-57 0.3601424403054949 1101 58-59 0.18156139448070974 1101 60-61 0.096402833003566 1101 62-63 0.26927264805934925 1101 64-65 0.10641003184412767 1101 66-67 0.13153062724659748 1101 68-69 0.09269616401197567 1101 70-71 0.07667332994861198 1101 72-73 0.16556212987222807 1101 74-75 0.18382522493926956 1101 76-77 0.2955926377239422 1101 78-79 0.34323489618546077 1101 80-81 0.3519606069584853 1101 82-83 0.22696442347614365 1101 84-85 0.2946143305270148 1101 86-87 0.3108228539298459 1101 88-89 0.39246318184289564 1101 90-91 0.48067657137435305 1101 92-93 0.4821531249575379 1101 94-95 0.3833928657861527 1101 96-97 0.5130838346350153 1101 98-99 0.6475638282443619 1101 100-101 0.9309784804102037 1102 1 0.051697160037949885 1102 2 -0.004895756081541691 1102 3 0.020182303182497918 1102 4 -0.11790564373491463 1102 5 -0.008456097624311099 1102 6 -0.02660385249690478 1102 7 -0.10618918687070789 1102 8 0.008001630361825107 1102 9 0.17906874507231407 1102 10-11 -0.09036311435797018 1102 12-13 0.16602152424530914 1102 14-15 0.17681252880411336 1102 16-17 0.24725962967749382 1102 18-19 0.16738953204220763 1102 20-21 0.20248959417143908 1102 22-23 0.18724395907035074 1102 24-25 0.2618136050954192 1102 26-27 0.23930686150588798 1102 28-29 0.17078144851340937 1102 30-31 0.017865801998944164 1102 32-33 0.012904912005701874 1102 34-35 0.22808070777735878 1102 36-37 0.2064951684103491 1102 38-39 0.3252670098205286 1102 40-41 0.4137923551413749 1102 42-43 0.31276337825724454 1102 44-45 0.21604785234436719 1102 46-47 0.4258672625153537 1102 48-49 0.5341981134519003 1102 50-51 0.48676993995206175 1102 52-53 0.3485569469629297 1102 54-55 0.38444957078580444 1102 56-57 0.46569202335059856 1102 58-59 0.4072948370371776 1102 60-61 0.562787066542846 1102 62-63 0.5799314339474009 1102 64-65 0.47107832615298406 1102 66-67 0.4907167008313209 1102 68-69 0.5812816044789386 1102 70-71 0.506549827180443 1102 72-73 0.4515739722770249 1102 74-75 0.18048313700774443 1102 76-77 0.352331343139781 1102 78-79 0.49754692643297815 1102 80-81 0.4055337235652239 1102 82-83 0.5154840296409482 1102 84-85 0.40859300922583586 1102 86-87 0.30482563906787163 1102 88-89 0.6589425410280647 1102 90-91 0.5070466798041231 1102 92-93 0.2744837136410325 1102 94-95 0.11977504556455543 1102 96-97 0.23761402712136004 1102 98-99 0.3714909712713279 1102 100-101 0.573481644574585 1103 1 0.15109186751534764 1103 2 0.09850976656427335 1103 3 0.15136887469422078 1103 4 0.03079488120123841 1103 5 0.1242416301424214 1103 6 0.11656276946969513 1103 7 0.12435033170697096 1103 8 0.12226485998235859 1103 9 0.2567947272590345 1103 10-11 0.2850688205808609 1103 12-13 0.3521117537096927 1103 14-15 0.3536986079579876 1103 16-17 0.3510744562317214 1103 18-19 0.3294287474029858 1103 20-21 0.3630592839979272 1103 22-23 0.4003254209233731 1103 24-25 0.4501574701797182 1103 26-27 0.3083182758454299 1103 28-29 0.2210965191455685 1103 30-31 0.33431789969444736 1103 32-33 0.33002893731510596 1103 34-35 0.38724908938763036 1103 36-37 0.4361915676253645 1103 38-39 0.3882018952232116 1103 40-41 0.3403007749222198 1103 42-43 0.41512372340056203 1103 44-45 0.35567067542081077 1103 46-47 0.41532548213153575 1103 48-49 0.510265117902371 1103 50-51 0.4693676502364781 1103 52-53 0.48953594588546423 1103 54-55 0.5370974921289076 1103 56-57 0.5705918424579863 1103 58-59 0.4992107995264661 1103 60-61 0.5284182479267301 1103 62-63 0.5737045934675038 1103 64-65 0.5410389468631394 1103 66-67 0.7067844211745538 1103 68-69 0.6910461224470694 1103 70-71 0.6633953183269696 1103 72-73 0.6912556490016399 1103 74-75 0.7200560550956006 1103 76-77 0.6067443292270838 1103 78-79 0.7251536190965844 1103 80-81 0.7087873265030851 1103 82-83 0.6833390955938619 1103 84-85 0.6310866453022648 1103 86-87 0.8264776342558271 1103 88-89 0.904301818038622 1103 90-91 0.9194885848466541 1103 92-93 0.8892793236620768 1103 94-95 1.009005554216742 1103 96-97 1.1073686211556897 1103 98-99 1.0982799639531393 1103 100-101 1.2406711377588202 1104 1 0.1617808071609801 1104 2 0.0858542510924245 1104 3 0.15576235581966102 1104 4 0.09528118449559742 1104 5 0.18522386242262456 1104 6 0.09325428990144502 1104 7 0.04169138674115658 1104 8 0.2043468831842219 1104 9 0.09580361925938519 1104 10-11 0.15621608872370274 1104 12-13 0.22394807178816478 1104 14-15 0.16832592490153075 1104 16-17 0.190119848971797 1104 18-19 0.21285588851274184 1104 20-21 0.25052054463369444 1104 22-23 0.3075293802295178 1104 24-25 0.24877107229854545 1104 26-27 0.26845145890425925 1104 28-29 0.185746209204396 1104 30-31 -0.10293706611302866 1104 32-33 0.01655178756596598 1104 34-35 0.304621655851804 1104 36-37 0.106598947392186 1104 38-39 0.23115037245961645 1104 40-41 0.1646056459065477 1104 42-43 0.27251861571287606 1104 44-45 0.2832173635432582 1104 46-47 0.2848660612380982 1104 48-49 0.23489552821795456 1104 50-51 0.07686902492260828 1104 52-53 0.10080753187148161 1104 54-55 0.07597878909734845 1104 56-57 0.24859413333894054 1104 58-59 0.1911912981528303 1104 60-61 0.1515488979917805 1104 62-63 0.19586403500093041 1104 64-65 0.2690110130308412 1104 66-67 0.31632957469036427 1104 68-69 0.27182068389986824 1104 70-71 0.20003483964734414 1104 72-73 0.47479129446864476 1104 74-75 0.49063196849379054 1104 76-77 0.48250972873161757 1104 78-79 0.5138201131506399 1104 80-81 0.6138230368311142 1104 82-83 0.5096943102891167 1104 84-85 0.648537281334221 1104 86-87 0.6246885465589926 1104 88-89 0.7877982842943432 1104 90-91 0.8631479862030105 1104 92-93 0.8151635212576487 1104 94-95 0.9567959727463027 1104 96-97 1.2568705304857524 1104 98-99 1.0985109164753908 1104 100-101 1.202426502973669 1105 1 0.18743575233850507 1105 2 0.15387873994272994 1105 3 0.10966742934770579 1105 4 0.15945101046802534 1105 5 0.21870597271969672 1105 6 0.0720520709747845 1105 7 0.04721267576650945 1105 8 0.19447526230663215 1105 9 0.2306756334115505 1105 10-11 0.20537398060036338 1105 12-13 0.2757579213856616 1105 14-15 0.29767092967087905 1105 16-17 0.24619679922042081 1105 18-19 0.33411759915704664 1105 20-21 0.19112321289443202 1105 22-23 0.2606254522030511 1105 24-25 0.22526955568876872 1105 26-27 0.248556198649446 1105 28-29 0.3158860576435245 1105 30-31 0.5099885106062629 1105 32-33 0.2969303894247943 1105 34-35 0.4446673043043745 1105 36-37 0.4890473175908383 1105 38-39 0.47237198339808373 1105 40-41 0.48061492432635333 1105 42-43 0.3910449944262808 1105 44-45 0.4800078139785029 1105 46-47 0.38637938180549725 1105 48-49 0.5198661470319266 1105 50-51 0.5001453024571916 1105 52-53 0.47374309061454767 1105 54-55 0.5701551791799631 1105 56-57 0.5998355224133434 1105 58-59 0.740341038520107 1105 60-61 0.704646257045745 1105 62-63 0.7647787544817604 1105 64-65 0.7086802619622077 1105 66-67 0.7593476638907575 1105 68-69 0.6573105681627354 1105 70-71 0.7696096883796386 1105 72-73 0.8093677091487947 1105 74-75 0.6829368006227519 1105 76-77 0.8423651180948966 1105 78-79 1.011612055169099 1105 80-81 0.8010830857115678 1105 82-83 0.8360405606663015 1105 84-85 0.8484923765281174 1105 86-87 0.691520340877819 1105 88-89 0.9466756355400214 1105 90-91 0.7177078976518771 1105 92-93 0.6799846099274838 1105 94-95 0.7643800226735848 1105 96-97 0.7841231715032926 1105 98-99 0.6945073812944784 1105 100-101 1.1785953507649651 1106 1 0.11352322948731342 1106 2 0.18499148312329794 1106 3 0.08919755300357934 1106 4 0.18457015597322624 1106 5 0.14235386083069557 1106 6 -0.0245543776509507 1106 7 0.130998509226238 1106 8 0.1426435747911654 1106 9 0.0821353155305431 1106 10-11 0.09950252203585563 1106 12-13 0.10010032600938956 1106 14-15 -0.040457228583086646 1106 16-17 -0.02093500724097197 1106 18-19 0.03135310411844472 1106 20-21 0.03705071175685504 1106 22-23 0.04609089801081012 1106 24-25 -0.044202851250013 1106 26-27 0.24203013831431974 1106 28-29 0.3627363853435597 1106 30-31 0.4396260049184164 1106 32-33 0.4325846319627331 1106 34-35 0.40559556154691023 1106 36-37 0.19775910920395035 1106 38-39 0.08653772537322624 1106 40-41 0.39788284005835095 1106 42-43 0.4562401376004601 1106 44-45 0.397874450842167 1106 46-47 0.5393406565227039 1106 48-49 0.6609200558826203 1106 50-51 0.4905873183113343 1106 52-53 0.6760238189787628 1106 54-55 0.7756410613356834 1106 56-57 0.4811769770662053 1106 58-59 0.5036945154932937 1106 60-61 0.5800915059939982 1106 62-63 0.8436415036495291 1106 64-65 0.6150410752649904 1106 66-67 0.49350111212532966 1106 68-69 0.4519292626313529 1106 70-71 0.6035571765449177 1106 72-73 0.6895101249486473 1106 74-75 0.6419954513448083 1106 76-77 0.5307451383715858 1106 78-79 0.6051785970356711 1106 80-81 0.5938417531978182 1106 82-83 0.5993812649220125 1106 84-85 0.8458284666396416 1106 86-87 0.5474967752140749 1106 88-89 0.6451612702243388 1106 90-91 0.7437570566482599 1106 92-93 0.9867573433688541 1106 94-95 1.0179487997657155 1106 96-97 1.0963019576161521 1106 98-99 1.244346524883774 1106 100-101 1.2403442551052954 1107 1 -0.009039357454970087 1107 2 -0.05079369293914482 1107 3 -0.014926751266429505 1107 4 0.11367402353415201 1107 5 0.1340169104938127 1107 6 0.10142409210348546 1107 7 0.07414749551945476 1107 8 0.1414126177396966 1107 9 0.22990844275346944 1107 10-11 0.05927679034032707 1107 12-13 0.19311273527117123 1107 14-15 0.32086164082595303 1107 16-17 0.20881409285365748 1107 18-19 0.21851609013253182 1107 20-21 0.24413293251012647 1107 22-23 0.3578891290276758 1107 24-25 0.16955270270367606 1107 26-27 0.28800636884960085 1107 28-29 0.15887158510184207 1107 30-31 0.14491470314988675 1107 32-33 0.16525207877744208 1107 34-35 0.17748431223132144 1107 36-37 0.22929283629222397 1107 38-39 0.2380547963518751 1107 40-41 0.2660781061036559 1107 42-43 0.3080570492264556 1107 44-45 0.3889191079272223 1107 46-47 0.21782697504702497 1107 48-49 0.23715565241213454 1107 50-51 0.04371064560695004 1107 52-53 0.09416563516954568 1107 54-55 0.2853354999434998 1107 56-57 0.2059677404405562 1107 58-59 0.22677795094626418 1107 60-61 0.12661527162443775 1107 62-63 0.163842174495052 1107 64-65 0.1800733675659103 1107 66-67 0.11927345102041187 1107 68-69 0.12073264538599204 1107 70-71 0.2612730503081693 1107 72-73 0.2538557744985823 1107 74-75 0.37429588093747057 1107 76-77 0.34387723849842544 1107 78-79 0.569986507307398 1107 80-81 0.4172293236910427 1107 82-83 0.42120506291376714 1107 84-85 0.5583914063356268 1107 86-87 0.6393587472913218 1107 88-89 0.5660889204398991 1107 90-91 0.680853556257933 1107 92-93 0.6885412035066096 1107 94-95 0.7373770933733574 1107 96-97 0.870942647295653 1107 98-99 0.7834008075437779 1107 100-101 1.0827469372604064 1108 1 -0.046897122796064394 1108 2 -0.12794581031916508 1108 3 -0.08584500817222462 1108 4 -0.14921117448550802 1108 5 0.03485657387926011 1108 6 0.16032789270342818 1108 7 -0.016119863727020345 1108 8 0.007394517657047572 1108 9 0.041477982620286014 1108 10-11 0.09055967644273011 1108 12-13 0.1731502272674632 1108 14-15 0.13802166759372625 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-1.3529196858044088 2316 78-79 -1.4814769280741729 2316 80-81 -1.2128681805040138 2316 82-83 -1.2802155130814832 2316 84-85 -1.4944775915326716 2316 86-87 -1.5715688694214407 2316 88-89 -1.4784946564539823 2316 90-91 -1.5505776782378042 2316 92-93 -1.687677508050811 2316 94-95 -1.6738599906470348 2316 96-97 -1.7499479045907407 2316 98-99 -1.6589022428521716 2316 100-101 -1.6343309468600289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6297.0 3 1154.0 4 953.0 5 1141.0 6 1357.0 7 1701.0 8 1965.0 9 2532.0 10 3345.0 11 4478.0 12 6062.0 13 7842.0 14 9299.0 15 11934.0 16 15270.0 17 20152.0 18 16820.0 19 11105.0 20 10611.0 21 11159.0 22 12193.0 23 13657.0 24 15261.0 25 17803.0 26 20507.0 27 24447.0 28 28698.0 29 35639.0 30 43240.0 31 54261.0 32 68450.0 33 90375.0 34 121862.0 35 171084.0 36 246814.0 37 348483.0 38 296574.0 39 40952.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.10304755817669 19.902820952325616 15.288520201218844 21.705611288278845 2 14.522104099122874 21.004018419384476 41.01641715605374 23.457460325438912 3 19.089008089613525 25.921000600968252 30.20451910252429 24.78547220689393 4 11.760060320031277 17.02745119941914 39.04214024407272 32.17034823647686 5 12.633351892629353 38.85054480003708 34.32298871308129 14.193114594252275 6 29.9762588819909 37.67521212973567 17.993356505200275 14.355172483073153 7 27.47944401158235 33.87607914753205 21.0485395927425 17.595937248143095 8 26.020588982187654 34.792812553195645 20.013785520738278 19.17281294387842 9 26.385969690441563 17.081915281915506 21.017280042505266 35.51483498513767 10-11 24.31947140635692 27.50756018847726 27.526537405769748 20.646430999396078 12-13 25.822145483031317 25.07400348898508 28.071232003554286 21.032619024429312 14-15 22.483702003082694 26.11962579062445 27.411104625271633 23.98556758102122 16-17 20.858052523610844 29.858121452234 27.671632969313926 21.61219305484123 18-19 20.780499945869146 28.276981440371795 30.519477620519275 20.423040993239784 20-21 22.023535964296187 26.862296499127858 30.32538653411119 20.788781002464766 22-23 21.371088244510478 27.08340716131929 30.35093383217334 21.194570761996896 24-25 21.55325968799316 27.08631415414629 30.491788001419636 20.868638156440916 26-27 21.172186051391726 27.181848927841518 30.744790451644878 20.90117456912188 28-29 20.61741489741982 27.84561948684377 30.68939026734945 20.847575348386965 30-31 20.954975782208436 27.2230526138359 30.63665052147229 21.185321082483377 32-33 20.905544113460927 27.347806187661515 30.499349754134503 21.247299944743055 34-35 21.56275384306719 27.199196575792183 30.288439671051474 20.94960991008915 36-37 21.365580317642618 27.002775515306354 30.55106549150227 21.08057867554876 38-39 20.985384709174323 27.572268027631864 30.49937181285352 20.942975450340292 40-41 21.148410263707472 27.50875049001349 30.404189632779357 20.93864961349968 42-43 20.982877116505417 27.575819210741958 29.815568596300768 21.625735076451857 44-45 21.19122265801043 27.48976223264951 29.67804227378197 21.640972835558088 46-47 21.475471495951908 27.916310445122537 29.30847632419546 21.299741734730095 48-49 21.489425883236706 27.931674465601574 29.14916219933592 21.429737451825797 50-51 21.15829568468174 28.161823946356357 29.27837658102709 21.401503787934814 52-53 21.495482300116826 28.493772568265076 28.624500351379762 21.386244780238336 54-55 21.196413744985172 28.02821559392988 28.886879469736616 21.888491191348333 56-57 21.574329870273353 28.30765565216809 28.532301650584518 21.585712826974035 58-59 21.92529659087866 27.974835524847574 28.61050297706721 21.489364907206557 60-61 21.608498070966238 28.054140720007076 29.316598394671185 21.020762814355503 62-63 21.352150241395616 28.195314930259613 29.217336962056038 21.23519786628873 64-65 21.155904169191437 28.49735136214864 28.922991426381035 21.42375304227889 66-67 21.286345814950934 28.357687573032024 28.41374157236909 21.94222503964795 68-69 21.130704786454977 28.23444897321516 28.852835410337313 21.78201082999255 70-71 21.51428958040095 28.222685048915324 28.721031288231448 21.54199408245228 72-73 21.423257906027267 27.96050502141832 29.09216573917197 21.52407133338244 74-75 21.0474318501086 28.805966193245624 28.506484719720888 21.640117236924887 76-77 21.305696752290622 28.201271872782584 28.60988283825574 21.883148536671047 78-79 21.328279333474704 28.285750957041934 28.607155213785866 21.778814495697496 80-81 21.379911996134346 28.545709895245714 28.434086934784965 21.640291173834974 82-83 21.337987847888783 28.368951846924475 28.724206898399064 21.56885340678768 84-85 21.24032923831279 28.418062578044008 28.546531805130737 21.79507637851247 86-87 21.216532563036054 28.43979927558652 28.44855941705443 21.895108744322997 88-89 21.293644414075807 28.257331603102255 28.277280784306942 22.171743198515 90-91 21.259634479845502 28.049930501732295 28.62084300757032 22.069592010851885 92-93 21.6489869122664 28.13638117623031 28.639025815259473 21.575606096243824 94-95 21.292810992559634 28.02220475617708 28.575900708044454 22.109083543218837 96-97 20.942876242087692 28.057566802667644 28.889782294279925 22.109774660964742 98-99 21.060800820971377 28.298680234941116 28.276861324642226 22.36365761944528 100-101 20.920742896900656 27.85444916282659 28.866985701460873 22.357822238811885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2599.0 1 1503.5 2 470.0 3 537.0 4 491.0 5 502.5 6 873.0 7 1747.0 8 2781.0 9 3278.0 10 2947.5 11 2532.5 12 2600.0 13 2805.5 14 2973.5 15 3092.5 16 3246.0 17 3386.5 18 3521.5 19 3793.0 20 4169.0 21 4734.5 22 5519.5 23 6556.5 24 7823.0 25 9525.0 26 12064.0 27 15094.0 28 17659.0 29 20509.0 30 24271.5 31 28011.5 32 32258.5 33 37234.0 34 42717.0 35 47137.5 36 51995.5 37 56724.0 38 58639.0 39 59182.0 40 59445.0 41 60915.5 42 62219.5 43 64417.5 44 67137.5 45 67910.0 46 66443.0 47 70167.0 48 82982.5 49 81026.0 50 66015.5 51 57215.0 52 51852.5 53 48802.5 54 45984.0 55 41458.5 56 36602.5 57 32220.0 58 28669.5 59 25542.5 60 21985.5 61 17927.5 62 13812.5 63 10136.0 64 7432.5 65 5704.0 66 4125.5 67 3032.0 68 2168.0 69 1428.0 70 1058.0 71 777.5 72 612.0 73 585.0 74 478.5 75 337.0 76 237.0 77 145.0 78 86.0 79 57.0 80 37.5 81 20.0 82 12.0 83 11.0 84 9.5 85 8.0 86 8.0 87 6.5 88 5.5 89 5.0 90 4.0 91 3.5 92 2.0 93 2.5 94 2.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31205879158672123 2 0.2779177886877992 3 0.28131518049340154 4 0.28098101080760457 5 0.2694521566476097 6 0.20423337296957106 7 0.21180788584763532 8 0.20941300309942384 9 0.20701812035121234 10-11 0.21570653218193314 12-13 0.21478756554599152 14-15 0.19810692872996027 16-17 0.2108889192116937 18-19 0.19721580956783505 20-21 0.20281315180493403 22-23 0.2007524387425195 24-25 0.19398550260513117 26-27 0.20545866181749325 28-29 0.20615484866290357 30-31 0.21370151406715138 32-33 0.21526097260087051 34-35 0.20409413560048903 36-37 0.19913728526116753 38-39 0.19122860269730632 40-41 0.19228680670233 42-43 0.19044887343044675 44-45 0.20732444256319288 46-47 0.1971044196725694 48-49 0.20554220423894248 50-51 0.21272685248357692 52-53 0.2220000612644424 54-55 0.21902038156608622 56-57 0.1861325149889028 58-59 0.1741581012478453 60-61 0.18248449591895272 62-63 0.18448951403373443 64-65 0.18117566464958132 66-67 0.19342855312880292 68-69 0.18100857980668283 70-71 0.18755273615353982 72-73 0.19888665799681982 74-75 0.18922358458252458 76-77 0.19885881052300342 78-79 0.1957677409293816 80-81 0.1838490221359569 82-83 0.19732719946310068 84-85 0.1802845454874561 86-87 0.18304144539528097 88-89 0.19167416227836892 90-91 0.18735780383682493 92-93 0.19345640060261934 94-95 0.19195263701653303 96-97 0.18663376951759822 98-99 0.1933450107073537 100-101 0.17900356169190113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1795495.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.39655765169655 #Duplication Level Percentage of deduplicated Percentage of total 1 73.93534057671887 37.26086653872432 2 14.043622028121359 14.155004143577074 3 4.977550060974786 7.525541658363647 4 2.28574106480841 4.607739253978692 5 1.2949804112929106 3.263127747777044 6 0.7473341927614957 2.2597842438353295 7 0.4888077233253953 1.7243958626413975 8 0.3525514558372346 1.4213903815432594 9 0.26819268932902085 1.216438949658019 >10 1.4278765718840003 13.331555827485037 >50 0.11665218563820462 4.124663359867953 >100 0.05692979753534989 4.989134582475834 >500 0.0024316829750993087 0.8350005837118941 >1k 0.0015474346205177419 1.639921093027229 >5k 4.4212417729078336E-4 1.6454357733332647 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9773 0.5443067232156035 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7369 0.41041606910629097 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6940 0.3865229365718089 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5457 0.3039273292323287 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4437 0.2471184826468467 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3259 0.18150983433537826 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2601 0.1448625587929791 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2567 0.14296893057346305 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2168 0.12074664646796567 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2141 0.11924288288187937 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2022 0.11261518411357313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.569494763282549E-5 0.0 16-17 0.0 0.0 0.0 1.6708484289847645E-4 0.0 18-19 0.0 0.0 0.0 1.6708484289847645E-4 0.0 20-21 0.0 0.0 0.0 1.6708484289847645E-4 0.0 22-23 0.0 0.0 0.0 2.2277979053130195E-4 0.0 24-25 0.0 0.0 0.0 4.455595810626039E-4 0.0 26-27 0.0 0.0 0.0 4.455595810626039E-4 0.0 28-29 0.0 0.0 0.0 0.0010582040050236842 0.0 30-31 0.0 0.0 0.0 0.0025619675911099727 0.0 32-33 0.0 0.0 0.0 0.00456698570589169 0.0 34-35 0.0 0.0 0.0 0.006404918977774931 0.0 36-37 0.0 0.0 0.0 0.007797292668595569 0.0 38-39 0.0 0.0 0.0 0.00902258151651773 0.0 40-41 0.0 0.0 0.0 0.011138989526565099 0.0 42-43 0.0 0.0 0.0 0.0142022116463705 0.0 44-45 0.0 0.0 0.0 0.017599603451972853 0.0 46-47 0.0 0.0 0.0 0.022472911369845083 0.0 48-49 0.0 0.0 0.0 0.02912845761196773 0.0 50-51 0.0 0.0 0.0 0.03957126029312251 0.0 52-53 0.0 0.0 0.0 0.06474537662315963 0.0 54-55 0.0 0.0 0.0 0.09724337856691331 0.0 56-57 0.0 0.0 0.0 0.13751082570544615 0.0 58-59 0.0 0.0 0.0 0.1851021584576955 0.0 60-61 0.0 0.0 0.0 0.25694864090404035 0.0 62-63 0.0 0.0 0.0 0.30860570483348604 0.0 64-65 0.0 0.0 0.0 0.36329814340892064 0.0 66-67 0.0 0.0 0.0 0.45945547049699387 0.0 68-69 0.0 0.0 0.0 0.5845184754065035 0.0 70-71 0.0 0.0 0.0 0.7026753068095428 0.0 72-73 0.0 0.0 0.0 0.8204422735791523 0.0 74-75 0.0 0.0 0.0 1.009415230897329 0.0 76-77 0.0 0.0 0.0 1.154194247268859 0.0 78-79 0.0 0.0 0.0 1.2957708041515015 0.0 80-81 0.0 0.0 0.0 1.4731035174144176 0.0 82-83 0.0 0.0 0.0 1.7304698704257042 0.0 84-85 0.0 0.0 0.0 1.9950208716816253 0.0 86-87 0.0 0.0 0.0 2.3161022447848643 0.0 88-89 0.0 0.0 0.0 2.7126224244567654 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTAC 45 9.6219394E-4 42.226303 9 GTACATG 9535 0.0 28.474972 1 TACATGG 9765 0.0 27.257992 2 CCGGTCC 105 6.9034126E-5 27.14548 9 ACATGGG 9955 0.0 25.830574 3 GAGTACT 7820 0.0 23.418509 12-13 CATGGGA 6545 0.0 22.439583 4 AGTACTT 8435 0.0 21.992653 12-13 CATGGGG 3280 0.0 21.446371 4 GTACTTT 8570 0.0 21.312426 14-15 GTATCAA 18655 0.0 21.079393 1 AGAGTAC 13880 0.0 20.860556 10-11 GTGTACG 185 6.4964097E-6 20.562037 1 GTCTACG 125 0.0059960955 19.019882 1 TACTTTT 9730 0.0 18.942455 14-15 ATGGGAG 2770 0.0 18.875702 5 ACTTTTT 9995 0.0 18.559305 16-17 CATTCCG 285 4.271351E-8 18.335104 9 CAACGCA 21340 0.0 18.197756 5 TCAACGC 21370 0.0 18.193436 4 >>END_MODULE