FastQCFastQC Report
Thu 26 May 2016
SRR937229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795495
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125000.6961868454103186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101030.5626860559344359No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81200.45224297477854297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74700.4160412588172064No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36830.20512449213169626No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33300.18546417561730888No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23870.13294383999955445No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23580.1313286865182025No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23450.13060465219897577No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21690.12080234141559848No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC21070.1173492546623633No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT20830.11601257591917549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG2000.033.2555775
GGACCGA2007.203198E-1026.123566
GAGTACT100500.024.0808612-13
GTACATG97700.023.7922131
AGTACTT105350.022.76935412-13
TACATGG99800.022.7543162
GTACTTT107600.022.3361314-15
ACATGGG97850.022.285293
GTATCAA211700.021.3765031
AGAGTAC170400.020.55825610-11
GTATACG1656.288724E-520.166691
CATGGGA62900.019.864224
TATAACG1456.1409414E-419.658472
TACTTTT120800.019.60053314-15
ACTTTTT123800.019.56677216-17
TTAGCCG1001.2851982E-519.00133394-95
GGTATCA163900.018.5908661
GTATATG9250.018.5004081
TCAACGC244100.018.4699134
CAACGCA244850.018.413345
Produced by FastQC (version 0.11.5)