##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937229_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1795495 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06516643042726 31.0 31.0 34.0 31.0 34.0 2 32.28066132180819 33.0 31.0 34.0 31.0 34.0 3 32.35484866290354 34.0 31.0 34.0 30.0 34.0 4 35.99544638108154 37.0 35.0 37.0 35.0 37.0 5 35.887864906335025 37.0 35.0 37.0 35.0 37.0 6 35.977225222013985 37.0 35.0 37.0 35.0 37.0 7 35.930401365640115 37.0 35.0 37.0 35.0 37.0 8 35.91301061824176 37.0 35.0 37.0 35.0 37.0 9 37.582432142668175 39.0 37.0 39.0 35.0 39.0 10-11 37.508103057931095 39.0 37.0 39.0 35.0 39.0 12-13 37.43272857902696 39.0 37.0 39.0 34.0 39.0 14-15 38.57318121186637 40.0 38.0 41.0 34.0 41.0 16-17 38.575264481382575 40.0 38.0 41.0 34.0 41.0 18-19 38.615346464345485 40.0 38.0 41.0 34.0 41.0 20-21 38.51972631502733 40.0 38.0 41.0 34.0 41.0 22-23 38.39745613326687 40.0 38.0 41.0 34.0 41.0 24-25 38.25363423457041 40.0 38.0 41.0 34.0 41.0 26-27 38.19316316670333 40.0 38.0 41.0 34.0 41.0 28-29 38.109226146550114 40.0 38.0 41.0 33.0 41.0 30-31 38.15490296547749 40.0 37.5 41.0 34.0 41.0 32-33 38.045056098736005 40.0 37.0 41.0 33.5 41.0 34-35 37.90712533312541 40.0 37.0 41.0 33.0 41.0 36-37 37.736520848011274 40.0 37.0 41.0 33.0 41.0 38-39 37.60186466684675 40.0 37.0 41.0 33.0 41.0 40-41 37.44069574128583 40.0 37.0 41.0 33.0 41.0 42-43 37.20530382986307 40.0 36.0 41.0 32.0 41.0 44-45 36.859075909428874 40.0 36.0 41.0 31.0 41.0 46-47 36.441897638255746 39.0 36.0 41.0 30.5 41.0 48-49 36.09178945081997 39.0 36.0 41.0 29.5 41.0 50-51 35.857528146834156 39.0 35.0 41.0 29.0 41.0 52-53 35.6411340605237 39.0 35.0 40.0 28.0 41.0 54-55 35.42929164380853 39.0 35.0 40.0 28.0 41.0 56-57 35.1834881188753 38.5 35.0 40.0 27.0 41.0 58-59 34.948230710751076 38.0 34.0 40.0 26.5 41.0 60-61 34.61191314929866 38.0 34.0 40.0 26.0 41.0 62-63 34.273650998749645 37.5 34.0 40.0 26.0 41.0 64-65 33.93617219763909 37.0 33.5 40.0 25.0 41.0 66-67 33.502705660556 36.5 33.0 39.0 23.5 40.5 68-69 33.10723811539436 36.0 32.5 39.0 22.5 40.0 70-71 32.68832578202668 35.5 32.0 38.5 22.0 40.0 72-73 32.240862826128726 35.0 32.0 38.0 21.0 40.0 74-75 31.795220816543626 35.0 31.0 37.0 20.0 39.0 76-77 30.46678520408021 33.5 29.5 36.0 18.5 38.5 78-79 31.1211211949908 34.0 31.0 36.0 19.0 39.0 80-81 30.967151676835634 34.0 31.0 36.0 18.0 37.5 82-83 30.622910116708763 34.0 31.0 35.5 17.0 37.0 84-85 30.23437185845686 34.0 30.5 35.0 12.0 37.0 86-87 29.863421786192667 34.0 30.0 35.0 7.0 36.0 88-89 29.43959576607008 34.0 29.5 35.0 4.5 36.0 90-91 28.969061735064706 34.0 29.0 35.0 2.0 35.5 92-93 28.59275687206035 34.0 29.0 35.0 2.0 35.0 94-95 28.145535632235124 33.5 29.0 35.0 2.0 35.0 96-97 27.623352891542446 33.0 27.5 35.0 2.0 35.0 98-99 27.0871430998137 33.0 27.0 35.0 2.0 35.0 100-101 25.089339987023077 31.0 21.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2194646209304878 1101 2 0.23927959626850281 1101 3 0.16496135740725748 1101 4 0.1189658990389546 1101 5 0.09671254972072774 1101 6 0.11047835561939934 1101 7 0.1082891831970656 1101 8 0.1244770255098615 1101 9 0.2008539155538429 1101 10-11 0.22361052663207204 1101 12-13 0.16709764479613654 1101 14-15 0.2099041430155637 1101 16-17 0.21291650072490853 1101 18-19 0.13248933432609533 1101 20-21 0.11866500126858881 1101 22-23 0.07625631131923427 1101 24-25 0.2620865919600348 1101 26-27 0.1594761834763503 1101 28-29 0.17187832521667445 1101 30-31 0.1770683741408945 1101 32-33 0.19340344088085004 1101 34-35 0.13985575707006603 1101 36-37 0.12415264471460574 1101 38-39 0.018034978652615052 1101 40-41 -0.0067686993688340635 1101 42-43 0.1504595665451518 1101 44-45 0.185183406705697 1101 46-47 0.31406031151833247 1101 48-49 0.2187953270474381 1101 50-51 0.18140785673263338 1101 52-53 0.14666792438800513 1101 54-55 0.15535400250949039 1101 56-57 0.05197578613867648 1101 58-59 -0.07628361665432948 1101 60-61 -0.014858822199606436 1101 62-63 0.07702654992941405 1101 64-65 -0.040711219142352206 1101 66-67 0.030599319118451263 1101 68-69 0.0016748556815784355 1101 70-71 -0.23106311743087105 1101 72-73 -0.23996538784837185 1101 74-75 -0.07286826507494482 1101 76-77 0.09487628522085245 1101 78-79 0.048496762255592074 1101 80-81 -0.08271269187162744 1101 82-83 -0.07707583374190818 1101 84-85 -0.12398224853080819 1101 86-87 -0.02904391588262456 1101 88-89 -0.10506448110865207 1101 90-91 0.03773860593666001 1101 92-93 0.009669514941641921 1101 94-95 -0.3227561923965325 1101 96-97 -0.2850693586103965 1101 98-99 -0.26660053176245313 1101 100-101 0.15278376840553065 1102 1 0.09109083955786446 1102 2 0.0945207914485664 1102 3 0.1752213562832594 1102 4 0.06895384322949383 1102 5 0.01659748731410815 1102 6 0.028232095445446248 1102 7 0.03096003577199724 1102 8 0.05325270172941288 1102 9 0.08878694378412177 1102 10-11 0.018800193909861207 1102 12-13 0.038231919061011865 1102 14-15 0.035639553867440554 1102 16-17 0.03918050249223626 1102 18-19 0.07564768187992854 1102 20-21 0.11980065593011346 1102 22-23 -0.026073425299529163 1102 24-25 0.06793367249880333 1102 26-27 0.11049903668113359 1102 28-29 0.11369745492449823 1102 30-31 0.15757370291226636 1102 32-33 0.09677373241388665 1102 34-35 0.1186763803515305 1102 36-37 -0.03830463226780978 1102 38-39 0.016759859900943752 1102 40-41 0.11057964929604935 1102 42-43 0.23000175138933088 1102 44-45 0.31084301228518996 1102 46-47 0.2634136099282358 1102 48-49 0.17134917740880695 1102 50-51 0.18538679182783113 1102 52-53 0.20751441055334396 1102 54-55 0.19549842359625558 1102 56-57 0.1307325952106595 1102 58-59 0.10698553557234902 1102 60-61 0.10562455489808542 1102 62-63 0.21687381020462482 1102 64-65 0.19258216124705285 1102 66-67 0.2844010428790611 1102 68-69 0.31257566421071914 1102 70-71 0.22381629987081197 1102 72-73 0.24082469629366443 1102 74-75 0.1951679898907912 1102 76-77 0.3235404160669191 1102 78-79 0.23706580889357198 1102 80-81 0.29539543436551696 1102 82-83 0.3277179756582065 1102 84-85 0.1642830331465106 1102 86-87 0.1158147281327544 1102 88-89 0.16241702533143254 1102 90-91 0.32273509278831725 1102 92-93 0.4333031488630361 1102 94-95 0.39984817841064313 1102 96-97 0.3679670349129758 1102 98-99 0.3770050073316469 1102 100-101 0.7701813244559794 1103 1 0.2153176883379686 1103 2 0.21565192277182632 1103 3 0.11844301899089515 1103 4 0.12117718309750813 1103 5 0.1053393991524274 1103 6 0.10756287058612202 1103 7 0.1383675546173535 1103 8 0.14539432606935065 1103 9 0.1995002790507101 1103 10-11 0.15809684482861286 1103 12-13 0.2679612597185823 1103 14-15 0.2993498082013204 1103 16-17 0.23064991389868794 1103 18-19 0.3065801633354539 1103 20-21 0.30760708267586523 1103 22-23 0.15887928687059372 1103 24-25 0.19270703796775734 1103 26-27 0.33247131606815117 1103 28-29 0.2733039317827419 1103 30-31 0.2510964037163035 1103 32-33 0.17750996587378864 1103 34-35 0.2838919807962128 1103 36-37 0.2282195215910363 1103 38-39 0.2921320333565731 1103 40-41 0.4295779648729976 1103 42-43 0.4216286073283584 1103 44-45 0.19619914040509912 1103 46-47 0.05964433075443765 1103 48-49 0.2523262465334497 1103 50-51 0.29133334472633976 1103 52-53 0.3040280387134544 1103 54-55 0.15676356693303006 1103 56-57 0.002244498484202495 1103 58-59 0.04481712231754642 1103 60-61 0.11346669328015935 1103 62-63 0.07932954027777583 1103 64-65 0.14602358228382428 1103 66-67 0.25469745879824046 1103 68-69 0.2607052127884586 1103 70-71 0.1360881803810443 1103 72-73 0.24169077091355717 1103 74-75 0.2918861403143893 1103 76-77 0.3861549653942653 1103 78-79 0.2565239558829582 1103 80-81 0.295269893749758 1103 82-83 0.1470405180320533 1103 84-85 0.3860867145991307 1103 86-87 0.246115252653901 1103 88-89 0.3336177227562409 1103 90-91 0.458366424613434 1103 92-93 0.3134739826654176 1103 94-95 0.41017752539712404 1103 96-97 0.26120563522848883 1103 98-99 0.46049963268888305 1103 100-101 0.8820023481515129 1104 1 0.09661281861409776 1104 2 0.10376691428212581 1104 3 0.11048506994382734 1104 4 0.059443665807449975 1104 5 0.10991722214327382 1104 6 0.1553210686785249 1104 7 0.1095437870162499 1104 8 0.07714424505871165 1104 9 0.1507489457233291 1104 10-11 0.06897333000169681 1104 12-13 0.07658512405978257 1104 14-15 0.1170123323403871 1104 16-17 0.11803515320961822 1104 18-19 0.05795865709960424 1104 20-21 -0.006059792141293485 1104 22-23 0.07719877180576873 1104 24-25 0.15499547153982718 1104 26-27 0.022833571467231195 1104 28-29 0.02997293204585816 1104 30-31 0.15826806380670888 1104 32-33 0.14695871542146222 1104 34-35 0.27530695429247487 1104 36-37 0.08785172543216646 1104 38-39 0.07146707390916163 1104 40-41 0.11118713280843906 1104 42-43 0.27071196608967085 1104 44-45 0.18604760193830572 1104 46-47 0.17323804653499764 1104 48-49 0.20218841028679435 1104 50-51 0.09142890313776775 1104 52-53 0.178562522984258 1104 54-55 0.17569103865021418 1104 56-57 0.19124703567950974 1104 58-59 0.15606704419545991 1104 60-61 0.2719618334298701 1104 62-63 0.35991860444607227 1104 64-65 0.26773135998748643 1104 66-67 0.3456848747086738 1104 68-69 0.22196703023118403 1104 70-71 0.17811300508558503 1104 72-73 0.13500488577587078 1104 74-75 0.07683559860664957 1104 76-77 0.10811399423299761 1104 78-79 0.08702830682365459 1104 80-81 0.08759197627714599 1104 82-83 0.18451561743748712 1104 84-85 0.14894127727037443 1104 86-87 0.14223703592773873 1104 88-89 0.13867580619955788 1104 90-91 0.03154988258322433 1104 92-93 0.06464226893205449 1104 94-95 0.14787296847671172 1104 96-97 0.40693759585390765 1104 98-99 0.5271226735313697 1104 100-101 0.9178154822205649 1105 1 0.029608555517697255 1105 2 0.08283841446016993 1105 3 0.06105946533779871 1105 4 0.0480464255939097 1105 5 -0.05240964766282019 1105 6 0.023308947241055478 1105 7 0.03811168122395969 1105 8 0.06671889357850347 1105 9 0.04343103542711191 1105 10-11 0.10907990026127834 1105 12-13 0.0376147598017198 1105 14-15 0.1356528321852224 1105 16-17 0.12439905088958625 1105 18-19 0.158908808474564 1105 20-21 0.15282227839929163 1105 22-23 0.11838563157263593 1105 24-25 0.0036132186628421437 1105 26-27 -0.0654023703967681 1105 28-29 -0.03208293582532207 1105 30-31 0.10992403578839571 1105 32-33 0.04142970391353629 1105 34-35 0.072863217583361 1105 36-37 0.05705360685774252 1105 38-39 -0.03460236837028674 1105 40-41 0.08753173858285379 1105 42-43 -0.0688558895056346 1105 44-45 -0.10092242481866265 1105 46-47 -0.006589398882070441 1105 48-49 0.251421953890933 1105 50-51 0.11503953556618995 1105 52-53 0.03257328011647331 1105 54-55 0.1253405952510036 1105 56-57 0.06489163273037946 1105 58-59 0.12081419724504627 1105 60-61 0.05009519498363346 1105 62-63 0.08377435419188117 1105 64-65 0.1405965583337334 1105 66-67 0.02268031415368199 1105 68-69 0.25312524530804836 1105 70-71 0.30806708491603274 1105 72-73 0.2813652368342119 1105 74-75 0.35881675860933626 1105 76-77 0.22779173789519902 1105 78-79 0.22125020234990345 1105 80-81 0.05822122989920331 1105 82-83 0.1545937146177785 1105 84-85 0.1353853171584305 1105 86-87 0.28682307510932503 1105 88-89 0.336980006229016 1105 90-91 0.46488612376493776 1105 92-93 0.32063012461315665 1105 94-95 0.32118120850863363 1105 96-97 0.30766722744858654 1105 98-99 0.4364545786821239 1105 100-101 0.924634434336884 1106 1 -0.07559233537934773 1106 2 0.08869949519172593 1106 3 0.09551227413820129 1106 4 0.03371862976579365 1106 5 0.033836530500622075 1106 6 -0.046918646618458126 1106 7 0.07742929879283622 1106 8 0.08103391668913673 1106 9 -0.026568246107530058 1106 10-11 -0.15125522391348767 1106 12-13 -0.17830084446756445 1106 14-15 -0.1198861365774988 1106 16-17 0.12178303869163898 1106 18-19 -0.05513000462066486 1106 20-21 -0.02116767905159378 1106 22-23 0.17658372045477932 1106 24-25 0.08155872705691536 1106 26-27 0.11795318214299755 1106 28-29 0.13724657633670034 1106 30-31 0.09323568211184607 1106 32-33 0.06704459051574929 1106 34-35 0.02755998528755299 1106 36-37 0.09933848635513698 1106 38-39 0.2933866470926816 1106 40-41 -0.007409467002013059 1106 42-43 -0.2269294443923897 1106 44-45 -0.21208418330254375 1106 46-47 -0.08517992950864794 1106 48-49 -5.70989182662629E-4 1106 50-51 0.10409012351033908 1106 52-53 0.06848856506781686 1106 54-55 -0.08202127978392326 1106 56-57 0.1397605128025461 1106 58-59 0.2678409481052171 1106 60-61 0.22575279035991258 1106 62-63 0.17913546286376203 1106 64-65 0.17883248639140703 1106 66-67 0.10581633561185555 1106 68-69 0.20818044739638708 1106 70-71 0.19136755511168957 1106 72-73 0.15686955960078564 1106 74-75 -0.027704831274675712 1106 76-77 -0.0322112956251992 1106 78-79 0.24622246160413752 1106 80-81 0.16995217260557993 1106 82-83 0.07266152178167573 1106 84-85 -0.16660163870384892 1106 86-87 0.04047088339083871 1106 88-89 -0.03155520256499855 1106 90-91 -0.20149209236540955 1106 92-93 -0.27545583577545685 1106 94-95 -0.7181914914043546 1106 96-97 -0.5604712288088471 1106 98-99 -0.29204374889018325 1106 100-101 0.28662282712987874 1107 1 -0.07036299179264205 1107 2 0.0633561354734411 1107 3 0.014002549638838957 1107 4 0.007960861132346508 1107 5 -0.018874688447468202 1107 6 -0.014032185765685767 1107 7 0.02581038935391433 1107 8 -0.025228995246671104 1107 9 -0.016728564996874695 1107 10-11 0.038141153152608354 1107 12-13 0.052907122244754134 1107 14-15 -0.07242404609770858 1107 16-17 -0.08994847586405541 1107 18-19 -0.014462717311978679 1107 20-21 0.0935473649736025 1107 22-23 0.03825902447479024 1107 24-25 0.010353705071160846 1107 26-27 -0.06683945235968736 1107 28-29 0.12322599699242431 1107 30-31 0.048102532926172614 1107 32-33 0.06839164954678267 1107 34-35 0.1801732000547389 1107 36-37 -0.009767313682594647 1107 38-39 0.02109207627891152 1107 40-41 0.03087167156423476 1107 42-43 0.07597847308777972 1107 44-45 0.21882256242184894 1107 46-47 0.21456348051538043 1107 48-49 -0.02486958445606291 1107 50-51 -0.12013270640256479 1107 52-53 -0.029170530686407403 1107 54-55 0.03807678574544582 1107 56-57 0.12746735300358125 1107 58-59 0.14804931818373035 1107 60-61 0.1038952507096198 1107 62-63 0.017596199076074015 1107 64-65 0.04636626255844334 1107 66-67 0.12123704041375305 1107 68-69 0.0655974007393425 1107 70-71 -0.0064311084118813255 1107 72-73 -0.010811452894365914 1107 74-75 0.03900764224962927 1107 76-77 0.11995162137185389 1107 78-79 0.18337908271445613 1107 80-81 0.23922073013434186 1107 82-83 0.24031768814645815 1107 84-85 0.34880510932111264 1107 86-87 0.3660533487711284 1107 88-89 0.22754668650776821 1107 90-91 0.4755399606764783 1107 92-93 0.5378161388630893 1107 94-95 0.5140882330796686 1107 96-97 0.6472345991424362 1107 98-99 0.734127355060199 1107 100-101 1.0206985280744938 1108 1 -0.058890098436393146 1108 2 -0.04242978501366679 1108 3 -0.08003595002843156 1108 4 -0.014425702969148801 1108 5 -0.14548750384968656 1108 6 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74-75 -0.6929465203819305 2316 76-77 -0.8713778048140313 2316 78-79 -1.019436150071268 2316 80-81 -0.9549917259157041 2316 82-83 -1.010833226332526 2316 84-85 -1.1422498568421453 2316 86-87 -0.9425736749625742 2316 88-89 -1.209037053244515 2316 90-91 -0.932339441226059 2316 92-93 -0.9469129781826595 2316 94-95 -1.0583930236636867 2316 96-97 -1.0733885450715839 2316 98-99 -0.9368290672316277 2316 100-101 -1.338089972668552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 18.0 5 36.0 6 92.0 7 197.0 8 474.0 9 896.0 10 1624.0 11 2613.0 12 4129.0 13 5979.0 14 7537.0 15 10928.0 16 15523.0 17 21845.0 18 18707.0 19 10468.0 20 8196.0 21 8266.0 22 8779.0 23 9555.0 24 10961.0 25 13185.0 26 16243.0 27 20536.0 28 25843.0 29 32074.0 30 40712.0 31 51479.0 32 65615.0 33 86572.0 34 118031.0 35 169108.0 36 256233.0 37 393399.0 38 329604.0 39 30027.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.74679249993313 20.388466373630294 15.897576208775044 20.967164917661535 2 15.270997691444421 21.787752124066063 40.009746615835745 22.93150356865377 3 19.45335408898382 25.9510051545674 30.76510934310594 23.83053141334284 4 12.411897554713324 17.892893046207313 38.57153598311329 31.123673415966067 5 12.611903508552045 38.91883009272025 34.36063692599927 14.108629472728436 6 30.005151782656036 38.20656699127539 17.448503059045 14.339778167023578 7 27.35969746504446 34.25473198198825 20.79198215533878 17.59358839762851 8 26.260501978563017 34.947187265907175 19.648453490541606 19.143857264988206 9 26.336692666924723 17.012077449394177 20.944363532062187 35.70686635161891 10-11 24.507419959398383 27.686042010698998 27.20620218936839 20.600335840534225 12-13 26.12752054644979 25.17856307409198 28.018501273222668 20.675415106235562 14-15 22.53932759489723 26.134770634281914 27.636835524465397 23.689066246355463 16-17 20.731525289683347 29.76282306550561 27.979220215038193 21.52643142977285 18-19 20.705822071350795 27.906705393220253 31.2502958794093 20.137176656019648 20-21 21.864722541694633 26.401159568809717 31.271042247402526 20.463075642093127 22-23 21.268033468468893 26.606575062615534 31.32400175774625 20.801389711169318 24-25 21.440295228107267 26.631265062019178 31.492074878598803 20.43636483127475 26-27 21.187863274140117 26.68022296037374 31.740134649530265 20.39177911595588 28-29 20.519926814800787 27.41632297285631 31.636669460774346 20.427080751568553 30-31 20.990284016385456 26.710489307962426 31.511727963597778 20.787498712054337 32-33 20.870706963817778 26.890133361552103 31.389338316174648 20.84982135845547 34-35 21.44567375570525 26.69213782271741 31.304431368508407 20.557757053068933 36-37 21.34934934377428 26.45768994065703 31.525178293451113 20.66778242211758 38-39 20.977752653168068 27.000465052812732 31.532669263907724 20.489113030111472 40-41 21.066948111802038 27.04351724733291 31.3288536030454 20.560681037819652 42-43 21.004904875603987 27.08437717326429 30.7129434851716 21.197774465960116 44-45 21.23765312629665 26.871559096516563 30.545810486801688 21.344977290385103 46-47 21.541747540371876 27.515504081047286 30.074241365194553 20.86850701338628 48-49 21.566114079961235 27.606593167900773 29.781174550750627 21.046118201387362 50-51 21.245812436124854 27.7444103158182 29.869089025589048 21.140688222467897 52-53 21.666281443278873 28.187714251501678 29.049175854012404 21.09682845120705 54-55 21.272518163514796 27.734134597979942 29.34962224901768 21.643724989487577 56-57 21.70278948145219 27.876602274024716 29.07192724012041 21.348681004402685 58-59 22.20312504351168 27.58231017073286 29.052907415503803 21.161657370251657 60-61 21.653945012378202 27.725418338675407 29.8352543449021 20.78538230404429 62-63 21.426236218981394 27.785429644749776 29.75555487483953 21.032779261429297 64-65 21.291175970971793 28.122411925402186 29.384877150869258 21.201534952756763 66-67 21.438739734724965 27.984622624958575 28.91511811506019 21.661519525256267 68-69 21.332780096853515 27.81319357614474 29.33143784861556 21.522588478386183 70-71 21.652831113425545 27.829122331167728 29.21712396859919 21.300922586807538 72-73 21.60787395522891 27.599751376922942 29.518448734578712 21.27392593326943 74-75 21.25221735510263 28.45137413359547 28.928791224704053 21.367617286597845 76-77 21.387277603112235 27.840010693429946 29.08390165386147 21.688810049596352 78-79 21.46104556125191 27.856747025193613 29.060036368800805 21.622171044753674 80-81 21.524426411658066 28.15265428196681 28.91024480714232 21.412674499232804 82-83 21.50364051241751 27.996495662184508 29.115383051636627 21.384480773761354 84-85 21.30814554159266 28.028352417440978 28.99405894714095 21.669443093825418 86-87 21.25650586607036 28.02580903873305 28.87265071749016 21.845034377706426 88-89 21.252579372262247 27.950175299847675 28.685348608600968 22.11189671928911 90-91 21.32990442674848 27.588299496831908 29.070510651417052 22.011285425002555 92-93 21.54918807046154 27.720142179566864 29.057586957441927 21.67308279252967 94-95 21.25347049142437 27.587823970548513 29.013447545105947 22.14525799292117 96-97 20.89334696003052 27.65365539865051 29.33775922517189 22.11523841614708 98-99 21.010787015480993 27.781131856894937 28.730871538274744 22.477209589349325 100-101 20.566908940919784 27.784949363037903 29.118864221420676 22.52927747462164 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 546.0 1 400.5 2 366.0 3 529.5 4 461.0 5 398.5 6 802.5 7 2013.0 8 3420.5 9 3990.5 10 3481.5 11 2895.5 12 2878.0 13 3041.0 14 3261.5 15 3430.5 16 3606.0 17 3839.0 18 4098.5 19 4359.5 20 4703.0 21 5180.5 22 5931.5 23 6956.0 24 8099.0 25 9689.5 26 12262.5 27 15190.5 28 17485.0 29 20390.0 30 24373.5 31 27892.5 32 31859.5 33 36744.0 34 42389.0 35 46943.5 36 51672.5 37 56368.5 38 58583.0 39 59404.0 40 59625.0 41 61201.5 42 62517.0 43 64332.5 44 66807.5 45 67742.0 46 66355.5 47 70929.0 48 84587.0 49 81570.5 50 66098.0 51 57227.0 52 51384.5 53 48843.5 54 45876.0 55 40897.0 56 35943.5 57 31269.5 58 28090.0 59 25476.5 60 21722.5 61 17433.0 62 13360.5 63 9819.5 64 7192.5 65 5457.5 66 3969.0 67 2950.5 68 2018.0 69 1240.0 70 858.0 71 667.0 72 588.0 73 500.0 74 394.0 75 322.0 76 227.0 77 128.0 78 75.0 79 57.0 80 37.0 81 20.0 82 11.0 83 5.0 84 2.5 85 1.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05296589519881704 2 0.0 3 0.0 4 0.0 5 0.0172654337661759 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0028404423292741 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.797292668595569E-4 24-25 1.1138989526565096E-4 26-27 0.0010582040050236842 28-29 0.0027847473816412743 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.013394634905694529 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.341696857969529E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.341696857969529E-4 84-85 0.0012252888479221606 86-87 0.0 88-89 0.0 90-91 0.009495988571396746 92-93 0.0010582040050236842 94-95 0.0 96-97 0.0 98-99 1.6708484289847645E-4 100-101 0.012002261214873891 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1795495.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.49212156294756 #Duplication Level Percentage of deduplicated Percentage of total 1 72.39030208470064 35.10359328669799 2 14.773318167580616 14.32779080940842 3 5.3583198615326495 7.795088942957928 4 2.4173574032581215 4.688911562395362 5 1.3624837642634986 3.303486416210398 6 0.8063673431117937 2.346147793594089 7 0.5336734250055551 1.8115269620198775 8 0.37002926443405815 1.4354803258227533 9 0.2771559449509861 1.2095891797011138 >10 1.514963163986233 13.596469596907232 >50 0.12970237568436271 4.356257637707784 >100 0.06159819353898878 5.3741605145788425 >500 0.0027681997771053526 0.928800427071848 >1k 0.0014994415459320658 1.5865003079545328 >5k 2.3068331475877937E-4 0.8719739044953698 >10k+ 2.3068331475877937E-4 1.2642223324765134 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12500 0.6961868454103186 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10103 0.5626860559344359 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8120 0.45224297477854297 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7470 0.4160412588172064 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3683 0.20512449213169626 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3330 0.18546417561730888 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2387 0.13294383999955445 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2358 0.1313286865182025 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2345 0.13060465219897577 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2169 0.12080234141559848 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2107 0.1173492546623633 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2083 0.11601257591917549 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.569494763282549E-5 0.0 10-11 0.0 0.0 0.0 5.569494763282549E-5 0.0 12-13 0.0 0.0 0.0 5.569494763282549E-5 0.0 14-15 0.0 0.0 0.0 1.1138989526565097E-4 0.0 16-17 0.0 0.0 0.0 1.6708484289847645E-4 0.0 18-19 0.0 0.0 0.0 1.6708484289847645E-4 0.0 20-21 0.0 0.0 0.0 1.6708484289847645E-4 0.0 22-23 0.0 0.0 0.0 1.6708484289847645E-4 0.0 24-25 0.0 0.0 0.0 3.8986463342977843E-4 0.0 26-27 0.0 0.0 0.0 4.177121072461912E-4 0.0 28-29 0.0 0.0 0.0 0.0010582040050236842 0.0 30-31 0.0 0.0 0.0 0.002729052434008449 0.0 32-33 0.0 0.0 0.0 0.0048454604440558175 0.0 34-35 0.0 0.0 0.0 0.006655546242122646 0.0 36-37 0.0 0.0 0.0 0.007964377511494045 0.0 38-39 0.0 0.0 0.0 0.009161818885599794 0.0 40-41 0.0 0.0 0.0 0.011222531948014336 0.0 42-43 0.0 0.0 0.0 0.014257906594003326 0.0 44-45 0.0 0.0 0.0 0.01787807819013698 0.0 46-47 0.0 0.0 0.0 0.022890623477091275 0.0 48-49 0.0 0.0 0.0 0.02965755961447957 0.0 50-51 0.0 0.0 0.0 0.040657311771962606 0.0 52-53 0.0 0.0 0.0 0.06572003820673408 0.0 54-55 0.0 0.0 0.0 0.09835727751956981 2.7847473816412744E-5 56-57 0.0 0.0 0.0 0.13870826707955186 5.569494763282549E-5 58-59 0.0 0.0 0.0 0.18621605741035202 5.569494763282549E-5 60-61 0.0 0.0 0.0 0.25861948933302514 5.569494763282549E-5 62-63 0.0 0.0 0.0 0.3109170451602483 5.569494763282549E-5 64-65 0.0 0.0 0.0 0.3660271958429291 5.569494763282549E-5 66-67 0.0 0.0 0.0 0.46265792998588134 5.569494763282549E-5 68-69 0.0 0.0 0.0 0.5882778843717192 5.569494763282549E-5 70-71 0.0 0.0 0.0 0.7064347157747585 5.569494763282549E-5 72-73 0.0 0.0 0.0 0.8238118179109382 5.569494763282549E-5 74-75 0.0 0.0 0.0 1.0150961155558773 5.569494763282549E-5 76-77 0.0 0.0 0.0 1.1617409126731069 5.569494763282549E-5 78-79 0.0 0.0 0.0 1.304654148298937 5.569494763282549E-5 80-81 0.0 0.0 0.0 1.4830172180930608 5.569494763282549E-5 82-83 0.0 0.0 0.0 1.7428619962740082 5.569494763282549E-5 84-85 0.0 0.0 0.0 2.0096129479614255 5.569494763282549E-5 86-87 0.0 0.0 0.0 2.335428391613455 5.569494763282549E-5 88-89 0.0 0.0 0.0 2.7359307600411027 5.569494763282549E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACCG 200 0.0 33.255577 5 GGACCGA 200 7.203198E-10 26.12356 6 GAGTACT 10050 0.0 24.08086 12-13 GTACATG 9770 0.0 23.792213 1 AGTACTT 10535 0.0 22.769354 12-13 TACATGG 9980 0.0 22.754316 2 GTACTTT 10760 0.0 22.33613 14-15 ACATGGG 9785 0.0 22.28529 3 GTATCAA 21170 0.0 21.376503 1 AGAGTAC 17040 0.0 20.558256 10-11 GTATACG 165 6.288724E-5 20.16669 1 CATGGGA 6290 0.0 19.86422 4 TATAACG 145 6.1409414E-4 19.65847 2 TACTTTT 12080 0.0 19.600533 14-15 ACTTTTT 12380 0.0 19.566772 16-17 TTAGCCG 100 1.2851982E-5 19.001333 94-95 GGTATCA 16390 0.0 18.590866 1 GTATATG 925 0.0 18.500408 1 TCAACGC 24410 0.0 18.469913 4 CAACGCA 24485 0.0 18.41334 5 >>END_MODULE