FastQCFastQC Report
Thu 26 May 2016
SRR937228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884762
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101650.5393253896247908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75200.3989893684189304No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT74370.3945856293792001No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55550.29473217308073907No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45350.24061393427923525No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33490.17768821739827098No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27540.14611924476406038No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27350.1451111599236402No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22510.11943152504135801No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21720.11524001438908468No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21500.11407275825807185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT1554.579124E-827.5878016
GTACATG104200.026.7242471
TGGACCG2007.166818E-1026.1310845
TACATGG105950.025.7846762
ACATGGG103950.025.0467193
GAGTACT82050.022.8977812-13
GTAAGCG1900.022.49955694-95
CATGGGA65500.022.4879444
AGTACTT85950.022.16276612-13
GTACTTT86050.021.69364414-15
GTATCAA194400.021.0710721
CATGGGG37050.020.2627454
AGAGTAC147600.020.1321210-11
TACTTTT99850.018.67163714-15
GGACCGA2051.5867208E-518.5413956
ATGGGAG27750.018.4907915
ATGGGAT30050.018.3403765
TCAACGC220900.018.3262354
CAACGCA220850.018.307415
ATCAACG221300.018.2916533
Produced by FastQC (version 0.11.5)