##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937228_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1884762 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.795634674298398 33.0 31.0 34.0 30.0 34.0 2 31.883853239825505 33.0 31.0 34.0 30.0 34.0 3 31.924789973482063 33.0 31.0 34.0 30.0 34.0 4 35.54197930560994 37.0 35.0 37.0 33.0 37.0 5 35.56248215955118 37.0 35.0 37.0 33.0 37.0 6 35.606869726787785 37.0 35.0 37.0 33.0 37.0 7 35.5056935570645 37.0 35.0 37.0 33.0 37.0 8 35.519063414903314 37.0 35.0 37.0 33.0 37.0 9 37.14197760778284 39.0 37.0 39.0 34.0 39.0 10-11 37.029057249668654 39.0 37.0 39.0 33.0 39.0 12-13 36.888207105194184 39.0 37.0 39.0 33.0 39.0 14-15 38.03651362877647 40.0 38.0 41.0 33.0 41.0 16-17 37.98519627411844 40.0 38.0 41.0 33.0 41.0 18-19 37.980987785195154 40.0 38.0 41.0 33.0 41.0 20-21 37.88522131706815 40.0 38.0 41.0 33.0 41.0 22-23 37.73365151674322 40.0 37.5 41.0 32.5 41.0 24-25 37.67241434196997 40.0 37.0 41.0 32.0 41.0 26-27 37.70463485575367 40.0 37.0 41.0 32.5 41.0 28-29 37.629589306236014 40.0 37.0 41.0 32.0 41.0 30-31 37.42237534500377 40.0 37.0 41.0 32.0 41.0 32-33 37.183201645618915 39.0 36.0 41.0 31.5 41.0 34-35 37.18021187821061 39.5 36.0 41.0 31.0 41.0 36-37 37.04160180436575 39.5 36.0 41.0 31.0 41.0 38-39 36.92906982420062 39.0 36.0 41.0 31.0 41.0 40-41 36.724732088189384 39.0 36.0 41.0 30.5 41.0 42-43 36.44833565192846 39.0 35.0 41.0 30.0 41.0 44-45 36.18055648405475 39.0 35.0 41.0 30.0 41.0 46-47 35.80944596718312 39.0 35.0 41.0 28.5 41.0 48-49 35.46042046688123 39.0 35.0 40.0 27.0 41.0 50-51 34.49410747882226 38.0 34.0 39.5 25.0 40.5 52-53 34.63354073352498 38.0 34.5 39.5 26.0 40.5 54-55 35.02040178017171 39.0 35.0 40.0 26.0 41.0 56-57 34.81013040373267 38.0 34.5 40.0 25.5 41.0 58-59 34.55158396656978 38.0 34.0 40.0 24.0 41.0 60-61 34.239711698347065 38.0 34.0 40.0 23.0 41.0 62-63 33.945273726868436 38.0 34.0 40.0 22.5 41.0 64-65 33.541833929164525 37.0 33.0 40.0 22.0 41.0 66-67 33.179758505317906 36.5 33.0 39.0 20.5 41.0 68-69 32.701298094931886 36.0 32.0 39.0 19.5 40.5 70-71 32.224041815359186 35.5 31.5 39.0 17.5 40.0 72-73 31.761839425879767 35.0 31.0 38.0 14.0 40.0 74-75 31.27173749258527 35.0 31.0 37.0 9.5 39.0 76-77 30.785178446933884 35.0 30.5 37.0 7.0 39.0 78-79 30.266608993602375 34.0 30.0 36.0 4.5 39.0 80-81 29.75005809752107 34.0 29.0 36.0 2.0 37.5 82-83 29.25892473426353 34.0 29.0 35.0 2.0 37.0 84-85 28.711538114626673 34.0 29.0 35.0 2.0 36.5 86-87 28.249393822668328 34.0 27.5 35.0 2.0 36.0 88-89 27.75784714462622 33.5 26.5 35.0 2.0 36.0 90-91 27.215244948699095 33.0 26.0 35.0 2.0 35.0 92-93 26.700014643758735 33.0 25.0 35.0 2.0 35.0 94-95 26.13390868449173 32.0 24.5 35.0 2.0 35.0 96-97 25.37082082512275 32.0 21.5 34.0 2.0 35.0 98-99 24.420396845861703 32.0 10.0 34.0 2.0 35.0 100-101 21.788929583682183 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07570777375720539 1101 2 -0.03211454451004059 1101 3 -0.04797474632257348 1101 4 -0.09189117375461109 1101 5 -0.050897386045676285 1101 6 -0.08088334116809648 1101 7 -0.029586776941997073 1101 8 -0.23341290603553233 1101 9 -0.2222883052744251 1101 10-11 -0.19176423461554037 1101 12-13 -0.10605588222915685 1101 14-15 -0.18240325636518406 1101 16-17 -0.3564030694653937 1101 18-19 -0.059395046984285216 1101 20-21 0.05762100076146481 1101 22-23 -0.07341600864740627 1101 24-25 -0.09616904824839168 1101 26-27 -0.08116594276226152 1101 28-29 -0.19740929224192882 1101 30-31 -0.2426356456821992 1101 32-33 -0.31492714483835726 1101 34-35 -0.0894668262746805 1101 36-37 -0.04967120101681388 1101 38-39 -0.15628934967520536 1101 40-41 -0.21993918958636272 1101 42-43 -0.1108530179835725 1101 44-45 0.009628480101277148 1101 46-47 -0.12736277097047122 1101 48-49 -0.26741037294945613 1101 50-51 -0.3231409760270125 1101 52-53 -0.3386382712724867 1101 54-55 -0.3272960602775896 1101 56-57 -0.18356547883765018 1101 58-59 -0.1456005416541828 1101 60-61 -0.2760722304818728 1101 62-63 -0.2959910111049311 1101 64-65 -0.3878635759878577 1101 66-67 -0.30964847781621785 1101 68-69 -0.4757734773001303 1101 70-71 -0.3700858737032853 1101 72-73 -0.2657160122970481 1101 74-75 -0.39267471230134987 1101 76-77 -0.4209296379576273 1101 78-79 -0.4902085142738706 1101 80-81 -0.3503283472830141 1101 82-83 -0.3417710121733357 1101 84-85 -0.4143385363345615 1101 86-87 -0.5067229813372158 1101 88-89 -0.4044943602668205 1101 90-91 -0.21220154687986437 1101 92-93 0.029237653257037977 1101 94-95 0.04979817151001953 1101 96-97 0.0533592328614354 1101 98-99 0.161247894116336 1101 100-101 0.5641425891346543 1102 1 -0.1095328980248631 1102 2 4.127259983448539E-4 1102 3 -0.011973693844964828 1102 4 -0.07565956290128639 1102 5 -0.0764069851434499 1102 6 -0.10233622764852157 1102 7 0.08526397636712346 1102 8 -0.171726120255407 1102 9 -0.14198106144150557 1102 10-11 -0.13341317967118727 1102 12-13 0.026562787650334485 1102 14-15 0.017730423870304435 1102 16-17 0.04452187312870848 1102 18-19 0.016260652623380167 1102 20-21 -0.0973130506108646 1102 22-23 -0.2270186671919987 1102 24-25 -0.17809785194661032 1102 26-27 -0.20230343217040314 1102 28-29 -0.14156912343005956 1102 30-31 -0.13033515986337818 1102 32-33 -0.27541076522283703 1102 34-35 -0.11350373569496242 1102 36-37 -0.2380714004336255 1102 38-39 -0.005111024828337918 1102 40-41 -0.1289234621434403 1102 42-43 -0.14265389718320165 1102 44-45 -0.12166875048047388 1102 46-47 -0.1071607835534607 1102 48-49 -0.20519691560379982 1102 50-51 -0.3791079441558125 1102 52-53 -0.33488362665245575 1102 54-55 -0.31220953813618024 1102 56-57 -0.2775231235381739 1102 58-59 -0.18258093493113137 1102 60-61 -0.10816245447092143 1102 62-63 -0.09679642478661776 1102 64-65 -0.09285068400269836 1102 66-67 -0.39585102436195285 1102 68-69 -0.3809113596563307 1102 70-71 -0.44609449086369324 1102 72-73 -0.4182205368255758 1102 74-75 -0.5113872188685136 1102 76-77 -0.33770146583253435 1102 78-79 -0.43452738556087667 1102 80-81 -0.4591717124763015 1102 82-83 -0.45005092101631305 1102 84-85 -0.3687719251480033 1102 86-87 -0.33965168203803486 1102 88-89 -0.22660443938439911 1102 90-91 -0.37727586512797373 1102 92-93 -0.4312576517061366 1102 94-95 -0.22450856512563888 1102 96-97 0.15888287941937662 1102 98-99 0.0524824261143344 1102 100-101 0.20322542760444406 1103 1 -0.05318963230410034 1103 2 -0.08869642625315421 1103 3 -0.03487116515498201 1103 4 -0.022688356601101134 1103 5 -0.1672908122004202 1103 6 -0.2834000397067342 1103 7 -0.11685570514877952 1103 8 -0.09310545566540185 1103 9 -0.07156538686119518 1103 10-11 -0.21366114659593194 1103 12-13 -0.22944424722442136 1103 14-15 -0.22839866643600004 1103 16-17 -0.20995957965802603 1103 18-19 -0.18985803074875918 1103 20-21 -0.25790649406504684 1103 22-23 -0.13197915807307226 1103 24-25 -0.19153268095907805 1103 26-27 -0.36874678706814024 1103 28-29 -0.3095659836207929 1103 30-31 -0.6082514505270993 1103 32-33 -0.4207603548846919 1103 34-35 -0.31779411397904056 1103 36-37 -0.3016246149707129 1103 38-39 -0.5651124520143753 1103 40-41 -0.3282625686363403 1103 42-43 -0.35438182056547163 1103 44-45 -0.5607631433236193 1103 46-47 -0.36299983597285035 1103 48-49 -0.35984182994604197 1103 50-51 -0.5048685190724385 1103 52-53 -0.38555582240920216 1103 54-55 -0.20616407561266215 1103 56-57 -0.43007645685968043 1103 58-59 -0.3397905734896227 1103 60-61 -0.27989504058519543 1103 62-63 -0.24447028884404887 1103 64-65 -0.2592437328089119 1103 66-67 -0.3193219407926762 1103 68-69 -0.18071527670579002 1103 70-71 -0.19663522594427718 1103 72-73 -0.1488591183622212 1103 74-75 -0.14087243907179214 1103 76-77 -0.15437676062179762 1103 78-79 -0.0648565070702567 1103 80-81 -0.12867842025065457 1103 82-83 -0.12805049374695443 1103 84-85 -0.20556433783701777 1103 86-87 0.0808655798213529 1103 88-89 -0.06922016998840874 1103 90-91 -0.05343848987378408 1103 92-93 0.14752855759911654 1103 94-95 -0.012302401454881817 1103 96-97 0.008209708566589313 1103 98-99 0.3489240347036926 1103 100-101 0.8606402795582682 1104 1 -0.10815910952335628 1104 2 -0.04890084045927878 1104 3 -0.030465431149927724 1104 4 -0.07590044756667425 1104 5 -0.06465848277475317 1104 6 -0.14561605006957734 1104 7 -0.03474419352808411 1104 8 -0.09995653060616405 1104 9 -0.0843835196058933 1104 10-11 0.009421006118898845 1104 12-13 0.019072437296969724 1104 14-15 0.08506503724164105 1104 16-17 -0.023066458927075928 1104 18-19 -0.09942497577626597 1104 20-21 -0.02058689698612426 1104 22-23 -0.3425701417001292 1104 24-25 -0.12820971632810085 1104 26-27 -0.17727698531574987 1104 28-29 -0.15496564547671454 1104 30-31 -0.07565346328065203 1104 32-33 -0.05730176688621924 1104 34-35 0.039482488247038816 1104 36-37 -0.07491438092723257 1104 38-39 -0.05387612893307647 1104 40-41 0.16079925284173413 1104 42-43 0.08008609787624721 1104 44-45 0.16034148689842453 1104 46-47 0.16996967457933465 1104 48-49 0.3031994945565373 1104 50-51 0.2733351923643639 1104 52-53 0.31860213680055693 1104 54-55 0.2892097069251349 1104 56-57 0.3445651446765936 1104 58-59 0.19283924207741876 1104 60-61 0.18541880285928158 1104 62-63 0.4214939171423566 1104 64-65 0.42007887719670833 1104 66-67 0.3895364248010722 1104 68-69 0.35521484635754774 1104 70-71 0.4514620654772088 1104 72-73 0.45915370185383253 1104 74-75 0.3756458806150995 1104 76-77 0.33624325969277535 1104 78-79 0.503838499378741 1104 80-81 0.6703446007685407 1104 82-83 0.6958235012882916 1104 84-85 0.6948056114135923 1104 86-87 0.7720741578662462 1104 88-89 0.5513942346530136 1104 90-91 0.8401042267416656 1104 92-93 0.8043036380842601 1104 94-95 0.8744195639288712 1104 96-97 0.8914185217938417 1104 98-99 0.955429928614226 1104 100-101 1.3430373664451842 1105 1 0.1128448083636826 1105 2 0.12170431739485466 1105 3 0.03850773852757783 1105 4 0.04830749324209194 1105 5 -0.0710400375670801 1105 6 4.5002883657474513E-4 1105 7 0.16869104514698563 1105 8 -0.02196594654819961 1105 9 0.016141886241655357 1105 10-11 0.039086219650712906 1105 12-13 0.14845194251849136 1105 14-15 0.11516107892089877 1105 16-17 0.024995162975905316 1105 18-19 0.14849570908712195 1105 20-21 0.1128381867860142 1105 22-23 0.1780276962582974 1105 24-25 0.16190575212198866 1105 26-27 0.10910010782586 1105 28-29 -0.04312309991907881 1105 30-31 0.012292295130258424 1105 32-33 0.047868403260693526 1105 34-35 0.10531484172131655 1105 36-37 0.07097636904837401 1105 38-39 0.01137202715034391 1105 40-41 -0.008664814603555726 1105 42-43 0.07543029563891679 1105 44-45 0.01529786961510382 1105 46-47 -0.27553997606411684 1105 48-49 -0.19750356399798363 1105 50-51 -0.18912280537692538 1105 52-53 -0.3267691329573523 1105 54-55 -0.30338290718390937 1105 56-57 -0.31364812687527177 1105 58-59 -0.009920242416022518 1105 60-61 0.11087414684790531 1105 62-63 0.03684978026263508 1105 64-65 0.06241971013113101 1105 66-67 0.11690226156972017 1105 68-69 0.001347501457999556 1105 70-71 -0.0026454417148684684 1105 72-73 0.038898388539060136 1105 74-75 0.02822016012822104 1105 76-77 0.13245306417022462 1105 78-79 0.13752372992059847 1105 80-81 0.17922096192057424 1105 82-83 0.2929716466823358 1105 84-85 0.4194013905061098 1105 86-87 0.5257112165230104 1105 88-89 0.3698758094298178 1105 90-91 0.7043195803971969 1105 92-93 0.8407549758823407 1105 94-95 0.9348798110857288 1105 96-97 1.1042872470165719 1105 98-99 1.2688170883552665 1105 100-101 1.2822117054469828 1106 1 -0.19694799414756758 1106 2 -0.14464066836760736 1106 3 -0.13214106561636996 1106 4 0.11454897744474124 1106 5 -7.892042094468366E-4 1106 6 -0.03770560252294075 1106 7 -0.4771008632662799 1106 8 -0.31380633062159546 1106 9 -0.43396598862349833 1106 10-11 -0.7483101573087723 1106 12-13 -0.4312316245437131 1106 14-15 -0.3858489116522037 1106 16-17 -0.324607109199178 1106 18-19 -0.5482408332804951 1106 20-21 -0.4196693207214963 1106 22-23 -0.3618347389924921 1106 24-25 -0.32680407420919977 1106 26-27 -0.49634174179075075 1106 28-29 -0.09878871699245906 1106 30-31 -0.351907098438069 1106 32-33 -0.27897688863314585 1106 34-35 0.03363928266711724 1106 36-37 -0.3063754973811612 1106 38-39 -0.06473320429469709 1106 40-41 -0.12105560345065669 1106 42-43 -0.2077409824352756 1106 44-45 0.05128628521516987 1106 46-47 -0.16275510381322533 1106 48-49 0.1359150487895633 1106 50-51 -0.04043333921173797 1106 52-53 0.07534511721515003 1106 54-55 -0.23199963070354102 1106 56-57 -0.1576812376559502 1106 58-59 -0.1344413654808463 1106 60-61 -0.21364649029408866 1106 62-63 -0.2734146103415611 1106 64-65 -0.24073344302203026 1106 66-67 -0.1202147097705506 1106 68-69 -0.16004131454246817 1106 70-71 0.11113047011929211 1106 72-73 -0.04190012842450486 1106 74-75 0.09268316682423716 1106 76-77 0.25252143351044154 1106 78-79 0.3171023376545463 1106 80-81 0.013782222209989925 1106 82-83 -0.02223263526952124 1106 84-85 0.07784770089417137 1106 86-87 0.15805156658180763 1106 88-89 0.5461226873757248 1106 90-91 0.5926897466799872 1106 92-93 0.5815406644835655 1106 94-95 0.7737133047735938 1106 96-97 0.8257385526911811 1106 98-99 0.9618856423461501 1106 100-101 1.4765436587595175 1107 1 0.18500932261391512 1107 2 0.12199408735986239 1107 3 0.198965449922472 1107 4 0.13303945602446277 1107 5 0.13062108560441033 1107 6 0.054433884509954567 1107 7 0.22849268268423373 1107 8 0.2611903318812665 1107 9 0.1368212090848715 1107 10-11 0.19062977419437033 1107 12-13 0.24271574870955703 1107 14-15 0.2539629378770485 1107 16-17 0.21863767251858235 1107 18-19 0.20998975725054692 1107 20-21 0.167659703890628 1107 22-23 0.3122957623566478 1107 24-25 0.3325279151922018 1107 26-27 0.24863637961275487 1107 28-29 0.25578412555757524 1107 30-31 0.25068669515721353 1107 32-33 0.32819461107678194 1107 34-35 0.28101146893370554 1107 36-37 0.22024941707220336 1107 38-39 0.3471309404648366 1107 40-41 0.4342523073356901 1107 42-43 0.40903915913416 1107 44-45 0.40679201404761756 1107 46-47 0.5400141006912307 1107 48-49 0.6405769987911256 1107 50-51 0.6657279534641845 1107 52-53 0.5930714454273414 1107 54-55 0.6688476717302763 1107 56-57 0.6860899008598551 1107 58-59 0.5687169241920813 1107 60-61 0.818965323304333 1107 62-63 0.8620608502454488 1107 64-65 0.8720131657582968 1107 66-67 0.7252501154910007 1107 68-69 0.7645013490929031 1107 70-71 0.9473637038488079 1107 72-73 0.9907853830661573 1107 74-75 1.0041367622143724 1107 76-77 0.8697829687194236 1107 78-79 0.9448774851371802 1107 80-81 1.069692247257347 1107 82-83 1.1259521136836952 1107 84-85 0.9358523341838811 1107 86-87 1.0071783334081275 1107 88-89 1.0875983474681412 1107 90-91 1.2452453761815718 1107 92-93 1.1021249572542722 1107 94-95 1.226230273899386 1107 96-97 1.3596230817417982 1107 98-99 1.7360375830821262 1107 100-101 2.0258171513894823 1108 1 0.13089633103073695 1108 2 0.05333748428819973 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64-65 1.020664075492924 2310 66-67 1.053791146619652 2310 68-69 0.8991958585737123 2310 70-71 1.089577778637775 2310 72-73 1.134143352779116 2310 74-75 1.2037559834130747 2310 76-77 0.9609202237597643 2310 78-79 1.073270427452531 2310 80-81 1.17041572403922 2310 82-83 1.0898278237660257 2310 84-85 1.297403555681342 2310 86-87 1.2597600366176813 2310 88-89 1.2990633504587343 2310 90-91 1.4185351650520914 2310 92-93 1.535112563784999 2310 94-95 1.466688308246816 2310 96-97 1.3995454556795757 2310 98-99 1.4716436209653416 2310 100-101 1.645410924601876 2311 1 0.33406276002321533 2311 2 0.3383619155802826 2311 3 0.26925087325024677 2311 4 0.2541179860211784 2311 5 0.3886985279521511 2311 6 0.3029588296337309 2311 7 0.33738479051714876 2311 8 0.33720491019383303 2311 9 0.4163978796336849 2311 10-11 0.48326409080835475 2311 12-13 0.4404266644001922 2311 14-15 0.4421208541973769 2311 16-17 0.5644727201237245 2311 18-19 0.4506390391645354 2311 20-21 0.4625849794936414 2311 22-23 0.4458389035899941 2311 24-25 0.441554933008689 2311 26-27 0.6678435441028441 2311 28-29 0.5704191471850208 2311 30-31 0.47204970779422695 2311 32-33 0.39070714381254135 2311 34-35 0.44134763247540576 2311 36-37 0.628495396470143 2311 38-39 0.6147543521341134 2311 40-41 0.6578107037748637 2311 42-43 0.7904449144677059 2311 44-45 0.7251921289387084 2311 46-47 0.39972060619793837 2311 48-49 0.5579652218525624 2311 50-51 0.5027725946086505 2311 52-53 0.4177055397578755 2311 54-55 0.5087282680257061 2311 56-57 0.46239190773792416 2311 58-59 0.5786177435944708 2311 60-61 0.6302093506920698 2311 62-63 0.5348472301620077 2311 64-65 0.5238309187739318 2311 66-67 0.6824108394788979 2311 68-69 0.7340764696833517 2311 70-71 0.8552998589544458 2311 72-73 0.8018188437549618 2311 74-75 0.6991978516643798 2311 76-77 0.7612090542844889 2311 78-79 0.9193796656239748 2311 80-81 0.8189153431770073 2311 82-83 0.9774856853468386 2311 84-85 1.2991670959137664 2311 86-87 1.205759301325326 2311 88-89 1.3877886274548992 2311 90-91 1.1320538913783622 2311 92-93 1.4250648098308005 2311 94-95 1.2869611914178307 2311 96-97 1.3309123625577648 2311 98-99 1.4771801400849434 2311 100-101 1.593557194915526 2312 1 0.4305068549108455 2312 2 0.37759983494193605 2312 3 0.3536543065932527 2312 4 0.4533042137500942 2312 5 0.3422141554977003 2312 6 0.44573440764312977 2312 7 0.5050973706023356 2312 8 0.4070492688531573 2312 9 0.5847175055611089 2312 10-11 0.5864087486796237 2312 12-13 0.614384147129968 2312 14-15 0.7176432219067479 2312 16-17 0.7376444519387988 2312 18-19 0.7211270869782922 2312 20-21 0.7392858482559461 2312 22-23 0.717619994137074 2312 24-25 0.704158402744369 2312 26-27 0.8080701311066463 2312 28-29 0.8811316265136284 2312 30-31 0.8499244658656551 2312 32-33 1.0106195006291685 2312 34-35 0.5268892141130479 2312 36-37 0.7443885183048948 2312 38-39 0.919003873540376 2312 40-41 0.9366557827685256 2312 42-43 0.8733690808574224 2312 44-45 0.8147825208717663 2312 46-47 0.790687202635759 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0.4601700839212626 2315 56-57 0.6917992308364944 2315 58-59 0.7043776181734742 2315 60-61 0.8047487762299212 2315 62-63 0.7003424551071191 2315 64-65 0.7406382730053807 2315 66-67 0.6287693041835212 2315 68-69 0.7450055736631 2315 70-71 0.729737849954212 2315 72-73 0.7090469264147607 2315 74-75 0.9120946441698479 2315 76-77 1.0060301817796677 2315 78-79 1.050900132455336 2315 80-81 1.019865401197361 2315 82-83 0.9127154115797218 2315 84-85 1.0577516317711542 2315 86-87 1.2464374824377806 2315 88-89 1.2408106388002018 2315 90-91 1.2194422476839826 2315 92-93 1.041426448471146 2315 94-95 1.4498048154459404 2315 96-97 1.5088872702710354 2315 98-99 1.599965713435207 2315 100-101 1.5253094904594846 2316 1 -0.11360325753025435 2316 2 -0.10315552124865945 2316 3 0.11562865936959454 2316 4 0.03577260372222213 2316 5 0.16132858881140777 2316 6 0.10014517285890889 2316 7 0.15094142923334886 2316 8 0.012766721303570705 2316 9 -0.012029138766180836 2316 10-11 -0.5175246908149376 2316 12-13 -0.07054243493985979 2316 14-15 -0.014937818288750293 2316 16-17 0.12665276708140993 2316 18-19 0.17592430119947267 2316 20-21 0.20424249498816494 2316 22-23 0.05559926501980783 2316 24-25 0.02383716354885479 2316 26-27 0.17210072719024794 2316 28-29 0.3628744789292284 2316 30-31 0.44602774609992224 2316 32-33 0.1911993771106424 2316 34-35 0.05139917180232345 2316 36-37 0.005330782220717367 2316 38-39 0.05263416307266766 2316 40-41 -0.05871488659460766 2316 42-43 -0.04472378130705579 2316 44-45 -0.3402912907037532 2316 46-47 -0.39887855120428384 2316 48-49 -0.39780902325265544 2316 50-51 -0.6547191245828543 2316 52-53 -0.3966784805333887 2316 54-55 -0.5448294447761057 2316 56-57 -0.5032152555180573 2316 58-59 -0.33913694755835877 2316 60-61 -0.36122509624055255 2316 62-63 -0.4127952917686457 2316 64-65 -0.511358251757855 2316 66-67 -0.4576899231778313 2316 68-69 -0.2870774227301922 2316 70-71 -0.1418588489429382 2316 72-73 -0.35199916698253375 2316 74-75 -0.2628919609132758 2316 76-77 -0.535581499580065 2316 78-79 -0.3272082944357031 2316 80-81 -0.3341653525395749 2316 82-83 -0.7771671472462849 2316 84-85 -0.43281022297833616 2316 86-87 -0.3858481740867852 2316 88-89 -0.46151772022325943 2316 90-91 -0.1984593145602105 2316 92-93 -0.03526255132545231 2316 94-95 0.15766477903161302 2316 96-97 0.33302193383537215 2316 98-99 0.4126164062500699 2316 100-101 0.48132951337967356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6727.0 3 1483.0 4 1051.0 5 1166.0 6 1340.0 7 1578.0 8 2126.0 9 2718.0 10 3507.0 11 4665.0 12 6439.0 13 8448.0 14 9807.0 15 12965.0 16 16307.0 17 21696.0 18 18204.0 19 12035.0 20 11969.0 21 12542.0 22 13652.0 23 15356.0 24 17082.0 25 20024.0 26 22980.0 27 26865.0 28 32135.0 29 38901.0 30 47473.0 31 58921.0 32 75462.0 33 97886.0 34 132480.0 35 184652.0 36 264315.0 37 361958.0 38 285246.0 39 32594.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11821208607686 19.883840908849155 15.226680424214702 21.77126658085928 2 14.48740478817446 21.05244127773273 40.990761295811346 23.46939263828147 3 19.068491169641668 25.832752476152955 30.263004687796574 24.835751666408804 4 11.758741387020088 16.992647031456283 39.0499575432397 32.198654038283934 5 12.61926954531751 38.8724737719989 34.3603696245957 14.14788705808789 6 30.006004689934084 37.60914414819298 17.98279648355997 14.402054678312965 7 27.424178360683637 33.876776041292715 21.08082234401551 17.618223254008143 8 26.00716549289221 34.74253629943826 20.06891083338075 19.181387374288782 9 26.290972709676048 17.072990449450153 21.058909237870377 35.57712760300342 10-11 24.360260272770542 27.483241018222813 27.48866558352634 20.6678331254803 12-13 25.8713984735918 25.059159552762022 28.037945079406363 21.03149689423982 14-15 22.474802798731872 26.09142673003766 27.444636348843908 23.989134122386556 16-17 20.83880670994313 29.87003766392209 27.687423483524558 21.603732142610223 18-19 20.753752263030258 28.302016725884123 30.46960686222297 20.474624148862656 20-21 22.044630411137582 26.862394306994464 30.279612054193727 20.813363227674227 22-23 21.40311669003626 27.038219253034455 30.343833431969497 21.214830624959788 24-25 21.411889063200775 27.16551059868556 30.51628664105425 20.90631369705941 26-27 21.16400653405707 27.119633650393915 30.76723059776413 20.949129217784886 28-29 20.614951306782164 27.856285871095928 30.669414745387012 20.859348076734896 30-31 20.965428078581034 27.239125758212733 30.586496547275168 21.208949615931065 32-33 20.909664302394283 27.31960435088518 30.538840313494326 21.23189103322621 34-35 21.514874868464894 27.220034661178605 30.282658534204977 20.982431936151524 36-37 21.312568078498746 26.99520349838074 30.576747349242268 21.115481073878247 38-39 20.970484641464008 27.545718284336036 30.498796159621577 20.985000914578382 40-41 21.118939124054215 27.48754459751902 30.399210936295333 20.99430534213143 42-43 20.98006883889009 27.594898073670265 29.803607634278638 21.621425453161 44-45 21.186413861305574 27.47608311060895 29.68353415534165 21.653968872743825 46-47 21.524507461440294 27.907411499688735 29.25229765345634 21.31578338541463 48-49 21.464410010549653 27.9152028035964 29.144169647579872 21.476217538274074 50-51 21.12885399291963 28.088087619566327 29.316434838479083 21.46662354903496 52-53 21.473298283934177 28.52021174333966 28.558486623474653 21.4480033492515 54-55 21.222236405412307 28.032348310782062 28.86049591524126 21.884919368564375 56-57 21.59182359354691 28.297538203612042 28.49823694042499 21.612401262416057 58-59 21.974313614209784 27.895446866821814 28.600087987102313 21.530151531866085 60-61 21.613159351825047 28.029438526064133 29.29174278955739 21.06565933255343 62-63 21.365575353707094 28.14257221079595 29.24725643236122 21.244596003135737 64-65 21.20601473620471 28.450938815696965 28.894237069537247 21.44880937856108 66-67 21.294883787198422 28.33389287300629 28.44266419565441 21.928559144140873 68-69 21.136814627656392 28.21430042943554 28.839726575427676 21.809158367480393 70-71 21.492316558634887 28.21785310229844 28.644956106772828 21.644874232293844 72-73 21.38631027113788 27.960135179499883 29.028157433662972 21.625397115699265 74-75 21.0234760030848 28.784069632883895 28.52399904191515 21.668455322116156 76-77 21.248440001680304 28.192539552305075 28.565104638864707 21.99391580714991 78-79 21.376176716193164 28.261260837327168 28.58345290250679 21.779109543972876 80-81 21.383839406934325 28.518901629011218 28.402754461058798 21.694504502995663 82-83 21.380771312426297 28.29589908650017 28.688211694626713 21.635117906446812 84-85 21.313848743481078 28.39181953467303 28.419944802168786 21.874386919677107 86-87 21.270303516249857 28.366421825032123 28.46906761941247 21.89420703930555 88-89 21.36309923369865 28.201750596492158 28.21288954759642 22.22226062221277 90-91 21.297241364091605 28.005555610206518 28.579780673569882 22.117422352131992 92-93 21.63166543005703 28.080095086463867 28.612018888625112 21.676220594853984 94-95 21.29740271167715 27.971883156982784 28.582958217905535 22.147755913434533 96-97 20.99064015232683 27.9841792360582 28.831446706081216 22.193733905533758 98-99 21.119036293981598 28.178186069302797 28.24627700007471 22.456500636640897 100-101 21.100081206912822 27.55862248620745 28.97757705685414 22.363719250025586 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2874.0 1 1685.0 2 558.5 3 606.0 4 526.0 5 532.5 6 901.0 7 1786.5 8 2824.0 9 3273.0 10 2954.0 11 2595.5 12 2641.5 13 2845.0 14 3022.5 15 3142.0 16 3259.5 17 3391.5 18 3623.0 19 3913.5 20 4304.0 21 4887.0 22 5727.5 23 6884.0 24 8159.5 25 9981.0 26 12802.0 27 15753.5 28 18223.5 29 21254.5 30 25214.0 31 29369.0 32 33831.5 33 38972.5 34 44799.0 35 49295.5 36 54557.5 37 59653.0 38 61364.0 39 62120.5 40 62773.0 41 64190.5 42 65536.0 43 67729.5 44 70168.0 45 71011.0 46 69950.5 47 74158.5 48 87248.5 49 85265.0 50 70021.5 51 60512.5 52 54456.5 53 51267.5 54 48145.5 55 43308.5 56 38578.5 57 34041.0 58 30259.0 59 26962.5 60 23037.5 61 18654.0 62 14495.5 63 10644.0 64 7688.0 65 5992.0 66 4395.5 67 3193.0 68 2280.5 69 1476.0 70 1081.0 71 829.5 72 648.5 73 578.0 74 454.5 75 346.5 76 234.5 77 134.0 78 94.0 79 66.5 80 42.0 81 35.0 82 30.5 83 22.0 84 15.5 85 10.5 86 6.0 87 4.0 88 5.5 89 4.5 90 3.0 91 2.5 92 1.0 93 0.0 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24310761783185356 2 0.21684435488406495 3 0.2080368768046045 4 0.21355481487848332 5 0.2145098426220393 6 0.24220564718516183 7 0.24018947750432149 8 0.2313819994248611 9 0.22506820489801896 10-11 0.23488376781789955 12-13 0.23692646604717202 14-15 0.22262757844226486 16-17 0.23599796685204816 18-19 0.2262089324805997 20-21 0.21957679537257224 22-23 0.21822383940253465 24-25 0.2140058002018292 26-27 0.22039918037396766 28-29 0.23440625394612158 30-31 0.23533475314124544 32-33 0.2339287400743436 34-35 0.24196689024927284 36-37 0.2186217676290163 38-39 0.21827689649939885 40-41 0.21498735649381728 42-43 0.21960332392100432 44-45 0.23602449540048026 46-47 0.24026906314961782 48-49 0.24435445960816274 50-51 0.24586658686879298 52-53 0.2620490014123799 54-55 0.24416875976913796 56-57 0.21660559794817597 58-59 0.2018557250199229 60-61 0.1993620414673046 62-63 0.20493303663804766 64-65 0.19888452759552663 66-67 0.1990436988861193 68-69 0.2018557250199229 70-71 0.2034209093774174 72-73 0.20456163695999813 74-75 0.2083286908373577 76-77 0.220240009083375 78-79 0.21496082794538515 80-81 0.21002651793701274 82-83 0.205012622283344 84-85 0.20644516389867795 86-87 0.21342217213632278 88-89 0.21087543148684026 90-91 0.2196829095663007 92-93 0.2094428898715063 94-95 0.20673697793143114 96-97 0.215889327140509 98-99 0.22222965021578323 100-101 0.19973344114535418 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1884762.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.09151477451045 #Duplication Level Percentage of deduplicated Percentage of total 1 73.94379225963905 37.03956562457041 2 13.912424269143884 13.937888116541572 3 5.01411016723067 7.534931205685743 4 2.3410526306906743 4.6906748975259385 5 1.2440584926336622 3.1158387182057146 6 0.7657590359892056 2.30148180389809 7 0.5272428046360293 1.8487273516725993 8 0.36221359793165253 1.4515062233857567 9 0.2673490625919366 1.2052727566897956 >10 1.4378044324907457 13.370522688113565 >50 0.11909446225599866 4.114505371773348 >100 0.06044213667357737 5.203154414432402 >500 0.002645822780117358 0.8765750536068343 >1k 0.001587493668070415 1.683066513571804 >5k 3.1749873361408294E-4 1.0874089809243053 >10k+ 1.0583291120469433E-4 0.5388802794020848 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10165 0.5393253896247908 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7520 0.3989893684189304 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7437 0.3945856293792001 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5555 0.29473217308073907 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4535 0.24061393427923525 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3349 0.17768821739827098 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2754 0.14611924476406038 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2735 0.1451111599236402 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2251 0.11943152504135801 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2172 0.11524001438908468 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2150 0.11407275825807185 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.305709686421946E-5 0.0 8 0.0 0.0 0.0 5.305709686421946E-5 0.0 9 0.0 0.0 0.0 1.591712905926584E-4 0.0 10-11 0.0 0.0 0.0 1.591712905926584E-4 0.0 12-13 0.0 0.0 0.0 2.1222838745687785E-4 0.0 14-15 0.0 0.0 0.0 2.1222838745687785E-4 0.0 16-17 0.0 0.0 0.0 3.183425811853168E-4 0.0 18-19 0.0 0.0 0.0 4.244567749137557E-4 0.0 20-21 0.0 0.0 0.0 5.040424202100849E-4 0.0 22-23 0.0 0.0 0.0 6.101566139385239E-4 0.0 24-25 0.0 2.652854843210973E-5 0.0 9.019706466917309E-4 0.0 26-27 0.0 5.305709686421946E-5 0.0 0.0011141990341486086 0.0 28-29 0.0 5.305709686421946E-5 0.0 0.0018039412933834617 0.0 30-31 0.0 5.305709686421946E-5 0.0 0.003422182747742155 0.0 32-33 0.0 5.305709686421946E-5 0.0 0.00687089404391642 0.0 34-35 0.0 5.305709686421946E-5 0.0 0.009391106144966844 0.0 36-37 0.0 5.305709686421946E-5 0.0 0.011221575986782417 0.0 38-39 0.0 5.305709686421946E-5 0.0 0.013184688570758538 0.0 40-41 0.0 5.305709686421946E-5 0.0 0.016315057285747486 0.0 42-43 0.0 5.305709686421946E-5 0.0 0.020453510841156604 0.0 44-45 0.0 5.305709686421946E-5 0.0 0.025626577785418 0.0 46-47 0.0 5.305709686421946E-5 0.0 0.03257705747463075 0.0 48-49 0.0 5.305709686421946E-5 0.0 0.04292319136315355 0.0 50-51 0.0 5.305709686421946E-5 0.0 0.057513893000813894 0.0 52-53 0.0 5.305709686421946E-5 0.0 0.08515664046707225 0.0 54-55 0.0 5.305709686421946E-5 0.0 0.12197826569084055 0.0 56-57 0.0 5.305709686421946E-5 0.0 0.16962353867490962 0.0 58-59 0.0 5.305709686421946E-5 0.0 0.22557224731822906 0.0 60-61 0.0 5.305709686421946E-5 0.0 0.30006441131559314 0.0 62-63 0.0 5.305709686421946E-5 0.0 0.36187592916240885 0.0 64-65 0.0 5.305709686421946E-5 0.0 0.4206897210363961 0.0 66-67 0.0 5.305709686421946E-5 0.0 0.5224267042735369 0.0 68-69 0.0 5.305709686421946E-5 0.0 0.657112144663358 0.0 70-71 0.0 5.305709686421946E-5 0.0 0.785483790526337 0.0 72-73 0.0 5.305709686421946E-5 0.0 0.9071702421844243 0.0 74-75 0.0 5.305709686421946E-5 0.0 1.0940903944370697 0.0 76-77 0.0 5.305709686421946E-5 0.0 1.2553309118074325 0.0 78-79 0.0 5.305709686421946E-5 0.0 1.416704071919956 0.0 80-81 0.0 5.305709686421946E-5 0.0 1.6046057804645892 0.0 82-83 0.0 1.0611419372843892E-4 0.0 1.8594124881550032 0.0 84-85 0.0 1.0611419372843892E-4 0.0 2.129552696839177 0.0 86-87 0.0 1.0611419372843892E-4 0.0 2.4454281230203074 0.0 88-89 0.0 1.0611419372843892E-4 0.0 2.8303838893186515 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 155 4.579124E-8 27.587801 6 GTACATG 10420 0.0 26.724247 1 TGGACCG 200 7.166818E-10 26.131084 5 TACATGG 10595 0.0 25.784676 2 ACATGGG 10395 0.0 25.046719 3 GAGTACT 8205 0.0 22.89778 12-13 GTAAGCG 190 0.0 22.499556 94-95 CATGGGA 6550 0.0 22.487944 4 AGTACTT 8595 0.0 22.162766 12-13 GTACTTT 8605 0.0 21.693644 14-15 GTATCAA 19440 0.0 21.071072 1 CATGGGG 3705 0.0 20.262745 4 AGAGTAC 14760 0.0 20.13212 10-11 TACTTTT 9985 0.0 18.671637 14-15 GGACCGA 205 1.5867208E-5 18.541395 6 ATGGGAG 2775 0.0 18.490791 5 ATGGGAT 3005 0.0 18.340376 5 TCAACGC 22090 0.0 18.326235 4 CAACGCA 22085 0.0 18.30741 5 ATCAACG 22130 0.0 18.291653 3 >>END_MODULE