FastQCFastQC Report
Thu 26 May 2016
SRR937228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884762
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128420.6813592379303063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105530.559911543208108No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT85740.45491154851381765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76500.4058867910112789No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38500.20426982292724494No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33980.18028801514461773No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26420.14017684991526783No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25400.13476502603511745No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.1330671989354624No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22760.12075795246296349No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21480.1139666440643434No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC21060.11173824599604619No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC702.0473279E-433.9468881
GTACATG101550.025.833731
TACATGG106700.024.306042
GGACCGA1401.780873E-523.7489516
ACATGGG105750.023.3116463
AATACCG1452.3356868E-522.9306325
GAGTACT103500.022.4663712-13
GTACTTT106600.021.7661614-15
AGTACTT105750.021.741312-13
CGTACAC1353.7976404E-421.1107393
CATGGGG36550.020.0133274
GGTATCA170450.019.7965431
AGAGTAC167300.019.69114910-11
CATGGGA67300.019.6207544
GTATCAA213550.019.161591
TACTTTT119700.019.0864614-15
GTACCGG1759.8999764E-519.0102561
ACTTTTT123950.018.91127816-17
CATTCCG2854.2733518E-818.334479
ATACCGT1700.00178866416.7639666
Produced by FastQC (version 0.11.5)