##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937228_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1884762 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16197058302321 33.0 31.0 34.0 31.0 34.0 2 32.36475852123504 34.0 31.0 34.0 31.0 34.0 3 32.41164030259524 34.0 31.0 34.0 31.0 34.0 4 36.06944537294364 37.0 35.0 37.0 35.0 37.0 5 35.99935270341825 37.0 35.0 37.0 35.0 37.0 6 36.051680265200595 37.0 35.0 37.0 35.0 37.0 7 35.99741399709884 37.0 35.0 37.0 35.0 37.0 8 35.97694616084153 37.0 35.0 37.0 35.0 37.0 9 37.67161583266216 39.0 37.0 39.0 35.0 39.0 10-11 37.58941951291463 39.0 37.0 39.0 35.0 39.0 12-13 37.49389976028804 39.0 37.0 39.0 35.0 39.0 14-15 38.646601003203585 40.0 38.0 41.0 34.0 41.0 16-17 38.63589965205156 40.0 38.0 41.0 34.0 41.0 18-19 38.665238104333596 40.0 38.0 41.0 34.0 41.0 20-21 38.59078440673146 40.0 38.0 41.0 34.0 41.0 22-23 38.44023754723408 40.0 38.0 41.0 34.0 41.0 24-25 38.376940695960556 40.0 38.0 41.0 34.0 41.0 26-27 38.32731347512312 40.0 38.0 41.0 34.0 41.0 28-29 38.21241833186365 40.0 38.0 41.0 34.0 41.0 30-31 38.24868312285583 40.0 38.0 41.0 34.0 41.0 32-33 38.157105247240764 40.0 37.0 41.0 34.0 41.0 34-35 38.0110536502752 40.0 37.0 41.0 33.5 41.0 36-37 37.83549090017732 40.0 37.0 41.0 33.0 41.0 38-39 37.7047680290668 40.0 37.0 41.0 33.0 41.0 40-41 37.53646959138608 40.0 37.0 41.0 33.0 41.0 42-43 37.304147685490264 40.0 36.5 41.0 32.5 41.0 44-45 36.96862654276774 40.0 36.0 41.0 31.5 41.0 46-47 36.567380921304654 39.5 36.0 41.0 30.5 41.0 48-49 36.19980639465354 39.0 36.0 41.0 30.0 41.0 50-51 35.949220379018676 39.0 35.0 41.0 29.0 41.0 52-53 35.71928657305273 39.0 35.0 40.0 28.0 41.0 54-55 35.508687569040546 39.0 35.0 40.0 28.0 41.0 56-57 35.249434676632916 38.5 35.0 40.0 27.0 41.0 58-59 34.99701394658848 38.0 34.5 40.0 26.5 41.0 60-61 34.6605022278675 38.0 34.0 40.0 26.0 41.0 62-63 34.32254868253923 37.5 34.0 40.0 26.0 41.0 64-65 33.94429349700387 37.0 33.5 40.0 25.0 41.0 66-67 33.519612025284886 36.5 33.0 39.0 23.5 40.5 68-69 33.089186857544874 36.0 32.5 39.0 22.5 40.0 70-71 32.67609517806493 35.5 32.0 38.5 22.0 40.0 72-73 32.20602335997861 35.0 31.5 38.0 20.5 40.0 74-75 31.731410650257168 35.0 31.0 37.0 19.5 39.0 76-77 30.47091383421355 33.5 29.5 36.0 19.0 38.5 78-79 31.08071947545632 34.0 31.0 36.0 19.0 39.0 80-81 30.873598629429075 34.0 31.0 36.0 18.0 37.5 82-83 30.51857263675732 34.0 31.0 35.5 15.5 37.0 84-85 30.13668940693838 34.0 30.0 35.0 10.0 37.0 86-87 29.729503247624898 34.0 30.0 35.0 7.0 36.0 88-89 29.247695730283187 34.0 29.0 35.0 2.0 36.0 90-91 28.709342081387465 34.0 29.0 35.0 2.0 35.0 92-93 28.354103595042773 34.0 29.0 35.0 2.0 35.0 94-95 27.893237979118847 33.0 27.5 35.0 2.0 35.0 96-97 27.385890101774123 33.0 27.0 35.0 2.0 35.0 98-99 26.61776606277079 33.0 25.5 34.5 2.0 35.0 100-101 24.060949074737287 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06562492213449644 1101 2 -0.08328352285133178 1101 3 0.012424072196814961 1101 4 -0.07167156652292306 1101 5 -0.060093895115876705 1101 6 -0.17720044444293848 1101 7 -0.06815087445359325 1101 8 -0.09595656280371401 1101 9 -0.1415676429324222 1101 10-11 -0.11662771096555247 1101 12-13 -0.14510723128632463 1101 14-15 -0.23282715431206213 1101 16-17 -0.18318973496182167 1101 18-19 -0.17481111276069328 1101 20-21 -0.09419036817616444 1101 22-23 -0.08310901448372476 1101 24-25 -0.14095929822639874 1101 26-27 -0.2009636867271638 1101 28-29 -0.29205380672652836 1101 30-31 -0.3332276116802504 1101 32-33 -0.06213636276607559 1101 34-35 -0.08015284568114112 1101 36-37 -0.10719048151002397 1101 38-39 -0.14809463853340077 1101 40-41 0.08046036252788724 1101 42-43 0.09892550562528868 1101 44-45 0.15852639080731734 1101 46-47 0.043316201325595216 1101 48-49 0.09709904164342475 1101 50-51 0.1067134823084146 1101 52-53 0.14441853757695355 1101 54-55 0.09982559983785677 1101 56-57 0.07560990733009021 1101 58-59 0.11750048129899682 1101 60-61 0.20154786580287976 1101 62-63 0.03848144361277406 1101 64-65 -0.010784392986408875 1101 66-67 0.07114013727070301 1101 68-69 0.022074843793184584 1101 70-71 0.21106080678833194 1101 72-73 0.23506800118311588 1101 74-75 0.07955611106280358 1101 76-77 0.23708302691000327 1101 78-79 0.051548676856828735 1101 80-81 0.036188566059042415 1101 82-83 0.09060190370007248 1101 84-85 0.2460158079783703 1101 86-87 0.20092490969829058 1101 88-89 0.27412140963634 1101 90-91 0.3282741120617416 1101 92-93 0.1684796910857571 1101 94-95 0.32345159083883246 1101 96-97 0.2808674793351251 1101 98-99 0.2013376258776347 1101 100-101 0.8154378321203986 1102 1 -0.07822652387385176 1102 2 -0.07655190723762217 1102 3 -0.16970561970248355 1102 4 -0.08258755959526809 1102 5 -0.1271763596791402 1102 6 -0.17582079863281308 1102 7 -0.0869738652037313 1102 8 -0.20048463998079313 1102 9 -0.24084761322762915 1102 10-11 -0.22809231664564322 1102 12-13 -0.21649459757363587 1102 14-15 -0.057771176524873624 1102 16-17 -0.22302938792118 1102 18-19 -0.20207274951917498 1102 20-21 -0.1345675117553995 1102 22-23 -0.10958908275628687 1102 24-25 -0.1710046776679519 1102 26-27 -0.2069790809432135 1102 28-29 -0.3149516861053172 1102 30-31 -0.34389887774329253 1102 32-33 -0.14416838024274625 1102 34-35 -0.3036316650020723 1102 36-37 -0.3919800162820266 1102 38-39 -0.34180158410664063 1102 40-41 -0.22302384900972783 1102 42-43 -0.27256381943017516 1102 44-45 -0.26682148506610304 1102 46-47 -0.265168360749378 1102 48-49 -0.13845881909198 1102 50-51 -0.1686807856700412 1102 52-53 -0.3602358196024369 1102 54-55 -0.3205861906810199 1102 56-57 -0.3378753082157715 1102 58-59 -0.30486155195617215 1102 60-61 -0.18103315376749407 1102 62-63 -0.11177855990125352 1102 64-65 -0.22704625391569522 1102 66-67 -0.1871957277213454 1102 68-69 -0.18704016621659747 1102 70-71 -0.11695344666662066 1102 72-73 -0.027314839277380543 1102 74-75 -0.020586787006990903 1102 76-77 -0.037671185813376695 1102 78-79 -0.028812445866730485 1102 80-81 -0.12676694858606297 1102 82-83 -0.07224736783136265 1102 84-85 -0.051278173424972096 1102 86-87 -0.024981921798833184 1102 88-89 -0.04962559240369657 1102 90-91 -0.13602971641210715 1102 92-93 0.1282663479893067 1102 94-95 0.08114696781363406 1102 96-97 0.022880637036255536 1102 98-99 0.1717697994948928 1102 100-101 0.7344825269321831 1103 1 -0.05011450483998203 1103 2 0.03630349311022485 1103 3 -0.001400052967795773 1103 4 -0.0567497304781881 1103 5 -0.04744053979179341 1103 6 -0.08555482788923996 1103 7 -0.06576641263329464 1103 8 -0.11597882752190003 1103 9 -0.06610641258300376 1103 10-11 -0.13548810990400995 1103 12-13 -0.11262293439663296 1103 14-15 -0.23693443774246958 1103 16-17 -0.21129277731958496 1103 18-19 -0.15644157871248154 1103 20-21 -0.13124952584287541 1103 22-23 -0.13849181603914928 1103 24-25 -0.15259530752680917 1103 26-27 -0.1738218263172655 1103 28-29 -0.2271952580062475 1103 30-31 -0.3331276122281892 1103 32-33 -0.15770958336943153 1103 34-35 -0.14791503145912088 1103 36-37 -0.18502430159868055 1103 38-39 -0.1337584157181837 1103 40-41 -0.10121329953423697 1103 42-43 -0.0061693523024288766 1103 44-45 -0.06729223484404656 1103 46-47 0.2061975006061445 1103 48-49 0.11741125911071748 1103 50-51 0.17191472321485435 1103 52-53 0.18293544981305843 1103 54-55 0.1868203286338641 1103 56-57 -0.05380833330055168 1103 58-59 -0.03071263351498743 1103 60-61 0.19732117663405546 1103 62-63 0.0831227515142885 1103 64-65 -0.07087287651983587 1103 66-67 0.09792811834113024 1103 68-69 -0.06301304613618441 1103 70-71 0.054571709924935874 1103 72-73 0.10370593558712926 1103 74-75 0.27713219740387984 1103 76-77 0.29240281511207655 1103 78-79 0.07498918768508034 1103 80-81 0.0021992545849478518 1103 82-83 0.008055780665127799 1103 84-85 0.04787579778664508 1103 86-87 0.13045703098346806 1103 88-89 0.012892247421707026 1103 90-91 -0.06131918697041172 1103 92-93 0.05306228698020732 1103 94-95 0.08140337375143858 1103 96-97 0.18229975459206216 1103 98-99 0.550382973574358 1103 100-101 1.1364093610435262 1104 1 0.04061912521817845 1104 2 0.011641058491377976 1104 3 0.007358869378506938 1104 4 -0.0027783893924819836 1104 5 -0.061837820194902804 1104 6 -0.01021939211207723 1104 7 -0.012995288582004605 1104 8 -0.0242167376211313 1104 9 0.01194658307538532 1104 10-11 -0.04151134758726016 1104 12-13 0.02317566247211289 1104 14-15 0.011535488082493828 1104 16-17 -0.12212602654898319 1104 18-19 -0.13819399743336902 1104 20-21 -0.1758084802258324 1104 22-23 -0.07951798887225436 1104 24-25 -0.052637945902453964 1104 26-27 -0.1396257101594074 1104 28-29 -0.16872975805392798 1104 30-31 -0.11465894564732082 1104 32-33 -0.045192534963540254 1104 34-35 -0.04857155133434787 1104 36-37 -0.01241642558237288 1104 38-39 -0.05517197740679336 1104 40-41 -0.13761367156680393 1104 42-43 -0.03904719729133177 1104 44-45 -0.055521427455623495 1104 46-47 0.0972541360355379 1104 48-49 0.059896210680747686 1104 50-51 0.031904950184348024 1104 52-53 0.022541431407724133 1104 54-55 -0.07800831231768512 1104 56-57 -0.030866595562216048 1104 58-59 -0.10373108329290659 1104 60-61 0.03637809076378318 1104 62-63 0.06384983535494371 1104 64-65 0.046069350101511475 1104 66-67 0.21341422823054756 1104 68-69 0.13703998563288167 1104 70-71 0.1206398961412134 1104 72-73 0.3321618662101855 1104 74-75 0.35753632216905373 1104 76-77 0.5065817185141697 1104 78-79 0.22527557312674062 1104 80-81 0.14302047618135916 1104 82-83 0.16179906894036478 1104 84-85 0.3361656146684808 1104 86-87 0.3171470291594183 1104 88-89 0.2539305497384383 1104 90-91 0.2543804851631677 1104 92-93 0.3608797623254105 1104 94-95 0.6141637433025444 1104 96-97 0.8195290055362641 1104 98-99 0.832639182527398 1104 100-101 1.2961492206563285 1105 1 0.07267004380699404 1105 2 0.02817031251267821 1105 3 -0.0017324410919599131 1105 4 0.03553022670571693 1105 5 0.002388571398604711 1105 6 -0.04678935869617362 1105 7 0.005363706988873673 1105 8 -0.0669619076518515 1105 9 -0.019537334068047585 1105 10-11 0.007807321167710768 1105 12-13 0.014643220229572762 1105 14-15 0.0360792145833031 1105 16-17 -0.031673145763363664 1105 18-19 0.02542495900512165 1105 20-21 -0.004870209022534766 1105 22-23 -0.04961226448143208 1105 24-25 -0.04799492443541453 1105 26-27 0.03970139390394678 1105 28-29 -0.027855808981811947 1105 30-31 -0.043334462803763074 1105 32-33 -0.08990757026169405 1105 34-35 -0.057120492389429955 1105 36-37 -0.10016814866258272 1105 38-39 -0.18123498892807532 1105 40-41 -0.15891503283501862 1105 42-43 -0.03988515106676971 1105 44-45 -0.1156443784657668 1105 46-47 -0.32398831750055734 1105 48-49 -0.07908223396922409 1105 50-51 -0.10884479303835803 1105 52-53 -0.19402930202258517 1105 54-55 -0.10016958704847667 1105 56-57 -0.10223418445828258 1105 58-59 -0.13313324533448423 1105 60-61 -0.05528675561135543 1105 62-63 -0.10060149779558714 1105 64-65 -0.06319461695662909 1105 66-67 -0.11384959475346079 1105 68-69 0.0499022042816506 1105 70-71 0.11529451325257156 1105 72-73 0.20626128666035015 1105 74-75 0.3392169469860562 1105 76-77 0.39386241211494877 1105 78-79 0.15850545967997576 1105 80-81 0.016209789598374158 1105 82-83 0.04252508640586683 1105 84-85 0.0875914838770484 1105 86-87 0.2143113247995494 1105 88-89 0.3285595588336996 1105 90-91 0.4832607270218148 1105 92-93 0.5769008064446304 1105 94-95 0.8501969175346531 1105 96-97 0.6396188659702524 1105 98-99 0.684142433490603 1105 100-101 1.1651524832384155 1106 1 -0.12159023589018148 1106 2 0.02048738686858087 1106 3 -0.06545976064452219 1106 4 0.028758892264477254 1106 5 -0.12756079544396215 1106 6 -0.0901856676264643 1106 7 -0.16493037349938078 1106 8 -0.09093879716272113 1106 9 -0.23019167773209404 1106 10-11 -0.20817322248274195 1106 12-13 -0.25140049338696 1106 14-15 -0.16294991163028527 1106 16-17 0.018821766624753877 1106 18-19 -0.06546593188576821 1106 20-21 -0.0370992827564649 1106 22-23 -0.16743129710629034 1106 24-25 -0.1276719315396022 1106 26-27 -0.14134883107831797 1106 28-29 -0.16452330171726715 1106 30-31 -0.12672294837671672 1106 32-33 -0.14294167232493038 1106 34-35 0.02704571265056188 1106 36-37 -0.10474043939992583 1106 38-39 -0.2190388121687974 1106 40-41 -0.10806952713515017 1106 42-43 -0.4424692886023678 1106 44-45 -0.17029393093085332 1106 46-47 -0.4227936762573705 1106 48-49 -0.3591504089403088 1106 50-51 -0.04533516690737116 1106 52-53 0.04590359203652383 1106 54-55 -0.013001107889699881 1106 56-57 -0.13051051904529487 1106 58-59 0.020898213308626623 1106 60-61 -0.005448081986266118 1106 62-63 0.03175658844599383 1106 64-65 0.18439520587590863 1106 66-67 0.2900360870448395 1106 68-69 0.35980846977044223 1106 70-71 0.4383447479532947 1106 72-73 0.3845794106095397 1106 74-75 0.395727097913678 1106 76-77 0.46254007035571476 1106 78-79 0.4919090088602225 1106 80-81 0.2273818840229005 1106 82-83 0.10036520808094096 1106 84-85 0.21948502790651503 1106 86-87 0.2520028998697619 1106 88-89 0.532113112387254 1106 90-91 0.6059370441156062 1106 92-93 0.6266680494157555 1106 94-95 0.5700813650890062 1106 96-97 0.7010319781115193 1106 98-99 0.6624396627587075 1106 100-101 1.3207661065704848 1107 1 0.013826043056091919 1107 2 0.030696654142801094 1107 3 0.016515124503385437 1107 4 0.03378895951566108 1107 5 -0.009386101166143135 1107 6 -0.044361211539239775 1107 7 -0.029578363085398962 1107 8 0.0424072409924392 1107 9 -0.0564666388607975 1107 10-11 0.015733877477906333 1107 12-13 0.031005114997832095 1107 14-15 0.018507698682057594 1107 16-17 0.02490817033138626 1107 18-19 0.07430944090893377 1107 20-21 0.15456548563116002 1107 22-23 0.12007199696616766 1107 24-25 0.001148882333268375 1107 26-27 -0.16011027968607294 1107 28-29 0.032045106055903716 1107 30-31 0.0729933767734181 1107 32-33 0.09717263633176998 1107 34-35 2.551640454413473E-4 1107 36-37 -0.09042294888015334 1107 38-39 0.0018149121554316139 1107 40-41 -0.11972123834065229 1107 42-43 -0.15033034747978036 1107 44-45 -0.07242834099783835 1107 46-47 -0.12584402457296306 1107 48-49 -0.16128893814874345 1107 50-51 -0.050079251596919505 1107 52-53 0.04783896816624633 1107 54-55 0.08641375069719714 1107 56-57 0.08556202350220588 1107 58-59 -0.14073429177950203 1107 60-61 -0.047875238939951714 1107 62-63 -0.018765549954181893 1107 64-65 0.04496611391831351 1107 66-67 0.09613916583012383 1107 68-69 0.2170362974990141 1107 70-71 0.22056933744114104 1107 72-73 0.1421891522301877 1107 74-75 0.1663317382505909 1107 76-77 0.3672568843437247 1107 78-79 0.2114988267395823 1107 80-81 0.4320112779185301 1107 82-83 0.364640142410412 1107 84-85 0.3862824649072252 1107 86-87 0.5199731507354066 1107 88-89 0.5418961974058369 1107 90-91 0.6446838970667272 1107 92-93 0.6016477390707067 1107 94-95 0.6532272278580713 1107 96-97 0.9219468692048949 1107 98-99 1.1872365220870478 1107 100-101 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0.05947891882630074 2316 24-25 0.16741457999263787 2316 26-27 0.12289469994234992 2316 28-29 0.25912487006677765 2316 30-31 0.12368860650874325 2316 32-33 0.02706864453708846 2316 34-35 -0.1650399106695275 2316 36-37 -0.12470282100273522 2316 38-39 0.02668080969001352 2316 40-41 -0.372418383033299 2316 42-43 -0.23073062367397768 2316 44-45 -0.3379284254087622 2316 46-47 -0.2818801861309552 2316 48-49 -0.645549484071779 2316 50-51 -0.51474886395156 2316 52-53 -0.5063045813398404 2316 54-55 -0.6903062091798873 2316 56-57 -0.5508562997887552 2316 58-59 -0.4694390585116608 2316 60-61 -0.4397008932067692 2316 62-63 -0.3251572848307589 2316 64-65 -0.5766597596115375 2316 66-67 -0.6421463024697758 2316 68-69 -0.58898000387304 2316 70-71 -0.31591220162263056 2316 72-73 -0.35323027148348984 2316 74-75 -0.4374766526471561 2316 76-77 -0.5163866608493919 2316 78-79 -0.6059375949930121 2316 80-81 -0.5770855909085348 2316 82-83 -0.6497394947992383 2316 84-85 -0.8056739338721641 2316 86-87 -1.0276305032102009 2316 88-89 -1.159592885242521 2316 90-91 -0.7724718121308598 2316 92-93 -0.38418621827364063 2316 94-95 -0.6359892457040601 2316 96-97 -0.7473175727649561 2316 98-99 -0.8153685099541903 2316 100-101 -0.8524549603954057 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 11.0 5 42.0 6 61.0 7 151.0 8 453.0 9 882.0 10 1542.0 11 2603.0 12 4123.0 13 5967.0 14 7615.0 15 11278.0 16 15532.0 17 22610.0 18 19387.0 19 10973.0 20 8887.0 21 9171.0 22 9563.0 23 10427.0 24 12114.0 25 14341.0 26 17233.0 27 21697.0 28 27590.0 29 34085.0 30 42715.0 31 54422.0 32 69519.0 33 91521.0 34 124582.0 35 181127.0 36 273735.0 37 415879.0 38 334452.0 39 28462.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79851057311503 20.356226395859846 15.829753388669163 21.015509642355962 2 15.33307653698451 21.760147965631734 39.96308287200188 22.94369262538188 3 19.39295253193772 25.963596464699524 30.728229877300155 23.9152211260626 4 12.342672443523373 17.848248213832836 38.53298188312371 31.276097459520084 5 12.583699257822364 38.96692566126393 34.348583665300595 14.10079141561311 6 30.025329458042975 38.17166305347837 17.478121906108036 14.32488558237061 7 27.433543333322724 34.231855268728886 20.77901613041859 17.5555852675298 8 26.260875378429745 34.948815818655085 19.633035895248312 19.157272907666854 9 26.325180579829176 17.000342748845743 20.88942794899303 35.78504872233205 10-11 24.502748360533968 27.66498121776778 27.21627687344808 20.615993548250174 12-13 26.173001175010818 25.106603910989943 28.050657318932533 20.669737595066707 14-15 22.591950601720537 26.178504235548044 27.519601944436484 23.709943218294935 16-17 20.75917277619137 29.825489902703893 27.926443763191322 21.488893557913414 18-19 20.74368010390702 27.930263874165544 31.096578772280004 20.229477249647434 20-21 21.869286413881433 26.47628719169847 31.19813005567812 20.456296338741975 22-23 21.33707565602289 26.62385810300849 31.136213206278295 20.90285303469032 24-25 21.486599377263072 26.720544880221166 31.313050654876562 20.4798050876392 26-27 21.20575915855354 26.726372055352567 31.592576115350834 20.47529267074306 28-29 20.59470924456247 27.43247276426916 31.527416130662534 20.445401860505836 30-31 20.96253461328885 26.81966814749731 31.367425864290972 20.85037137492287 32-33 20.92704543066976 26.955578476221405 31.28556815131035 20.831807941798488 34-35 21.478467838379594 26.729024672611185 31.154835464636914 20.637672024372307 36-37 21.392447106025646 26.46929769875332 31.409075209005277 20.729179986215758 38-39 21.027024331130033 27.0459520805456 31.37709272230847 20.5499308660159 40-41 21.1386233302569 27.056967775977665 31.217102880820008 20.587306012945426 42-43 21.020001740481476 27.148990520743567 30.60546468732887 21.225543051446085 44-45 21.242234410059226 26.963794271622284 30.404184730445355 21.389786587873132 46-47 21.599172733745693 27.531327562843476 29.93171021062606 20.937789492784763 48-49 21.566807904658518 27.613141606208103 29.67173043599139 21.14832005314199 50-51 21.261729597689257 27.73928485400279 29.792037403131005 21.206948145176952 52-53 21.702660601179353 28.16530681327404 28.988514199670835 21.143518385875776 54-55 21.31834152004338 27.712915476861273 29.282742330331363 21.68600067276399 56-57 21.66968561547824 27.907794193643547 29.030243606354546 21.39227658452367 58-59 22.202511510737164 27.5634005778979 29.02995179232179 21.204136119043145 60-61 21.75545241255925 27.68954382569258 29.78659905070242 20.768404711045747 62-63 21.508020641332966 27.802688084755527 29.651038168214345 21.038253105697166 64-65 21.323865115029143 28.163510343351135 29.296359247575886 21.216265294043836 66-67 21.46180790996423 27.951301013072204 28.893117539508967 21.693773537454597 68-69 21.31770483488101 27.818976613492847 29.27730397790278 21.586014573723368 70-71 21.724546653635844 27.85983057807829 29.114524804723356 21.30109796356251 72-73 21.644032509144388 27.579158535666572 29.3591976069127 21.41761134827633 74-75 21.26692919318195 28.465158996202174 28.792043769982627 21.475868040633248 76-77 21.433979462658943 27.81298116154719 29.05889443866122 21.694144937132645 78-79 21.495817509054195 27.866542300831615 28.982784033209498 21.654856156904692 80-81 21.503035267894923 28.190042283909435 28.816090328766087 21.490832119429562 82-83 21.56436413683915 27.971277934031676 29.025898884363098 21.438459044766077 84-85 21.299994110615376 28.100562038300104 28.900881656795313 21.698562194289206 86-87 21.315874365039196 28.03659029628144 28.76286236670731 21.88467297197206 88-89 21.334126006360485 27.916946542852628 28.60127697820733 22.14765047257956 90-91 21.339789540933076 27.651890101557658 28.915238795401343 22.093081562107923 92-93 21.607221260537084 27.82408457523362 28.884219741737798 21.684474422491494 94-95 21.304865017439866 27.633197188822777 28.93524487441916 22.126692919318195 96-97 20.975672259945817 27.678534478093255 29.219153399739596 22.12663986222133 98-99 21.06473559749549 27.860750366492482 28.611668653658107 22.462845382353926 100-101 20.764452476951572 27.68674141956935 29.03657582428195 22.51223027919713 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 525.0 1 382.5 2 371.0 3 526.5 4 434.0 5 379.0 6 821.5 7 2103.0 8 3569.0 9 4204.5 10 3667.0 11 2899.5 12 2861.0 13 3018.0 14 3178.5 15 3436.0 16 3622.0 17 3798.5 18 4029.0 19 4248.0 20 4671.5 21 5321.5 22 6150.5 23 7238.5 24 8396.0 25 10101.5 26 12682.5 27 15793.5 28 18376.5 29 21157.0 30 25092.0 31 28960.0 32 33399.0 33 38565.0 34 44248.0 35 48671.0 36 54028.5 37 59290.5 38 61478.5 39 62582.5 40 62948.5 41 64107.0 42 65626.0 43 68105.5 44 70729.0 45 71436.0 46 69843.0 47 74672.0 48 88998.0 49 85827.5 50 69402.0 51 60363.0 52 54527.0 53 51388.5 54 48200.5 55 43145.5 56 37939.0 57 33382.0 58 29708.5 59 26602.5 60 22902.0 61 18326.5 62 14041.0 63 10410.5 64 7529.5 65 5750.5 66 4118.5 67 3037.5 68 2205.5 69 1351.5 70 941.0 71 696.5 72 565.0 73 519.5 74 421.0 75 330.0 76 224.5 77 136.0 78 102.0 79 61.0 80 34.0 81 31.5 82 22.0 83 15.5 84 10.5 85 5.0 86 3.5 87 2.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07178625205728893 2 0.0 3 0.0 4 0.0 5 0.0020161696808403398 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.0611419372843892E-4 12-13 0.005226124041125617 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.693279045311821E-4 24-25 9.284991951238405E-4 26-27 3.9792822648164597E-4 28-29 0.003156897263421058 30-31 4.7751387177797516E-4 32-33 0.0 34-35 0.0 36-37 5.305709686421946E-5 38-39 8.489135498275114E-4 40-41 1.8569983902476812E-4 42-43 0.011990903891313598 44-45 2.652854843210973E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.652854843210973E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3264274216054866E-4 82-83 4.7751387177797516E-4 84-85 7.958564529632919E-4 86-87 0.0 88-89 0.0 90-91 0.00888706372475676 92-93 0.0026263262947788632 94-95 0.0 96-97 0.0 98-99 1.591712905926584E-4 100-101 0.010478776630683343 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1884762.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.04361636452723 #Duplication Level Percentage of deduplicated Percentage of total 1 72.2084164680169 34.69153459079415 2 14.7774257723702 14.199219493260628 3 5.357973488181993 7.722492682725701 4 2.507158741000969 4.818118908704866 5 1.3470594789921146 3.2358804414448543 6 0.8371792180687216 2.4132670308749127 7 0.5490953050920323 1.846636692978325 8 0.3706598635795556 1.4246272230035335 9 0.2851119357429603 1.2328027615604271 >10 1.5605016324378684 13.82248170089844 >50 0.12815562949738868 4.247474837012864 >100 0.06650490043264908 5.690116269723925 >500 0.0028766681686384784 0.9818440632592119 >1k 0.0014383340843192392 1.567516625058248 >5k 2.2128216681834448E-4 0.8624025814691924 >10k+ 2.2128216681834448E-4 1.2435840972307541 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12842 0.6813592379303063 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10553 0.559911543208108 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8574 0.45491154851381765 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7650 0.4058867910112789 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3850 0.20426982292724494 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3398 0.18028801514461773 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2642 0.14017684991526783 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2540 0.13476502603511745 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2508 0.1330671989354624 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2276 0.12075795246296349 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2148 0.1139666440643434 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2106 0.11173824599604619 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0611419372843892E-4 0.0 5 0.0 0.0 0.0 1.0611419372843892E-4 0.0 6 0.0 0.0 0.0 1.0611419372843892E-4 0.0 7 0.0 0.0 0.0 1.0611419372843892E-4 0.0 8 0.0 0.0 0.0 1.0611419372843892E-4 0.0 9 0.0 0.0 0.0 1.0611419372843892E-4 0.0 10-11 0.0 0.0 0.0 1.0611419372843892E-4 0.0 12-13 0.0 0.0 0.0 1.0611419372843892E-4 0.0 14-15 0.0 0.0 0.0 1.591712905926584E-4 0.0 16-17 0.0 0.0 0.0 1.8569983902476812E-4 0.0 18-19 0.0 0.0 0.0 2.1222838745687785E-4 0.0 20-21 0.0 0.0 0.0 2.9181403275320704E-4 0.0 22-23 0.0 0.0 0.0 3.9792822648164597E-4 0.0 24-25 0.0 0.0 0.0 6.632137108027432E-4 0.0 26-27 0.0 0.0 0.0 8.754420982596211E-4 0.0 28-29 0.0 0.0 0.0 0.0016447700027908034 0.0 30-31 0.0 0.0 0.0 0.003342597102445826 0.0 32-33 0.0 0.0 0.0 0.006764779850187981 0.0 34-35 0.0 0.0 0.0 0.009284991951238407 0.0 36-37 0.0 0.0 0.0 0.011141990341486087 0.0 38-39 0.0 0.0 0.0 0.013211217119190646 0.0 40-41 0.0 0.0 0.0 0.016474228576340143 0.0 42-43 0.0 0.0 0.0 0.020533096486452934 0.0 44-45 0.0 0.0 0.0 0.02565310633385011 0.0 46-47 0.0 0.0 0.0 0.03260358602306286 0.0 48-49 0.0 0.0 0.0 0.04292319136315355 0.0 50-51 0.0 0.0 0.0 0.05740777880708546 0.0 52-53 0.0 0.0 0.0 0.08510358337020801 5.305709686421946E-5 54-55 0.0 0.0 0.0 0.12184562294868 5.305709686421946E-5 56-57 0.0 0.0 0.0 0.16954395302961328 5.305709686421946E-5 58-59 0.0 0.0 0.0 0.22586406135098225 5.305709686421946E-5 60-61 0.0 0.0 0.0 0.30091332486542066 5.305709686421946E-5 62-63 0.0 0.0 0.0 0.3628309569059648 5.305709686421946E-5 64-65 0.0 0.0 0.0 0.4220692055548658 5.305709686421946E-5 66-67 0.0 0.0 0.0 0.5239653600825993 5.305709686421946E-5 68-69 0.0 0.0 0.0 0.6592078999894947 5.305709686421946E-5 70-71 0.0 0.0 0.0 0.7883488737570048 5.305709686421946E-5 72-73 0.0 0.0 0.0 0.9115474526757225 5.305709686421946E-5 74-75 0.0 0.0 0.0 1.0991573471876024 5.305709686421946E-5 76-77 0.0 0.0 0.0 1.26145900649525 5.305709686421946E-5 78-79 0.0 0.0 0.0 1.4232035662858227 5.305709686421946E-5 80-81 0.0 0.0 0.0 1.6128561590269754 5.305709686421946E-5 82-83 0.0 0.0 0.0 1.869758622043526 5.305709686421946E-5 84-85 0.0 0.0 0.0 2.141145672504009 5.305709686421946E-5 86-87 0.0 0.0 0.0 2.4595147822377577 5.305709686421946E-5 88-89 0.0 0.0 0.0 2.8504660004817586 5.305709686421946E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 70 2.0473279E-4 33.946888 1 GTACATG 10155 0.0 25.83373 1 TACATGG 10670 0.0 24.30604 2 GGACCGA 140 1.780873E-5 23.748951 6 ACATGGG 10575 0.0 23.311646 3 AATACCG 145 2.3356868E-5 22.930632 5 GAGTACT 10350 0.0 22.46637 12-13 GTACTTT 10660 0.0 21.76616 14-15 AGTACTT 10575 0.0 21.7413 12-13 CGTACAC 135 3.7976404E-4 21.110739 3 CATGGGG 3655 0.0 20.013327 4 GGTATCA 17045 0.0 19.796543 1 AGAGTAC 16730 0.0 19.691149 10-11 CATGGGA 6730 0.0 19.620754 4 GTATCAA 21355 0.0 19.16159 1 TACTTTT 11970 0.0 19.08646 14-15 GTACCGG 175 9.8999764E-5 19.010256 1 ACTTTTT 12395 0.0 18.911278 16-17 CATTCCG 285 4.2733518E-8 18.33447 9 ATACCGT 170 0.001788664 16.763966 6 >>END_MODULE