FastQCFastQC Report
Thu 26 May 2016
SRR937227_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937227_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148754
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.39931699315648655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.2904123586592629No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.19024698495502643No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2200.14789518265055057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCT250.005004384657.023869
GGTATCA4050.051.6439481
TATAGGG405.361851E-447.5198862
GTAAGGT554.9301525E-543.1998984
GTATCAA6350.036.681251
TAAGGTG851.6180373E-533.543455
GTAGTAG600.00392541331.6906051
TAGAGGT753.0581182E-431.6799224
AGGTAAG753.0581182E-431.6799222
TATAAGC600.003931900531.6799222
TATGAGT953.4607023E-530.0125584
ATCAACG7800.028.633783
TCAACGC7900.028.2713224
TATCAAC8200.027.2370072
CAACGCA8200.027.2370075
GTCACGG350.008364622527.1542246-47
GAGTACT5550.026.96165812-13
AACGCAG8400.026.5885076
GGTAAGG908.888966E-426.3999373
GTAGCCC908.888966E-426.3999373
Produced by FastQC (version 0.11.5)