##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937227_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 148754 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.782083170872717 33.0 31.0 34.0 30.0 34.0 2 31.896715382443496 33.0 31.0 34.0 30.0 34.0 3 31.937702515562606 34.0 31.0 34.0 30.0 34.0 4 35.54258036758675 37.0 35.0 37.0 33.0 37.0 5 35.54748107613913 37.0 35.0 37.0 33.0 37.0 6 35.58676741465776 37.0 35.0 37.0 33.0 37.0 7 35.53097731825699 37.0 35.0 37.0 33.0 37.0 8 35.511818169595436 37.0 35.0 37.0 33.0 37.0 9 37.143108756739316 39.0 37.0 39.0 34.0 39.0 10-11 37.03826787851083 39.0 37.0 39.0 33.0 39.0 12-13 36.89614733049196 39.0 37.0 39.0 33.0 39.0 14-15 38.02240948142571 40.0 38.0 41.0 33.0 41.0 16-17 37.92896325476961 40.0 38.0 41.0 33.0 41.0 18-19 37.87671255899001 40.0 38.0 41.0 33.0 41.0 20-21 37.727284644446534 40.0 38.0 41.0 33.0 41.0 22-23 37.814929346437744 40.0 38.0 41.0 33.0 41.0 24-25 37.789511542546755 40.0 38.0 41.0 33.0 41.0 26-27 37.70558774890087 40.0 38.0 41.0 33.0 41.0 28-29 37.56071769498635 40.0 37.0 41.0 32.5 41.0 30-31 37.295185339553896 40.0 37.0 41.0 32.0 41.0 32-33 37.04195517431464 39.0 36.5 41.0 31.0 41.0 34-35 37.05430778332011 40.0 37.0 41.0 31.0 41.0 36-37 36.90981082861637 40.0 36.0 41.0 31.0 41.0 38-39 36.778375707544 39.0 36.0 41.0 30.5 41.0 40-41 36.55977990507819 39.0 36.0 41.0 30.0 41.0 42-43 36.29276859781922 39.0 35.0 41.0 30.0 41.0 44-45 36.04172997028651 39.0 35.0 40.5 29.5 41.0 46-47 35.659605792113155 39.0 35.0 40.0 27.5 41.0 48-49 35.31775616117886 39.0 35.0 40.0 26.5 41.0 50-51 34.36106592091642 38.0 34.0 39.5 25.0 40.5 52-53 34.47913333422967 38.0 34.0 39.5 25.0 40.5 54-55 34.78017061726071 38.0 34.0 40.0 25.5 41.0 56-57 34.52550855775307 38.0 34.0 40.0 24.0 41.0 58-59 34.27025491751482 38.0 34.0 40.0 23.0 41.0 60-61 33.926637266897025 38.0 33.5 40.0 22.5 41.0 62-63 33.559658899928735 37.0 33.0 40.0 21.5 41.0 64-65 33.14896070021646 37.0 32.5 39.5 20.0 41.0 66-67 32.707833066673835 36.0 32.0 39.0 18.0 40.5 68-69 32.20857590384124 36.0 31.0 39.0 14.5 40.0 70-71 31.7254292321551 35.0 31.0 38.5 9.5 40.0 72-73 31.221980585396025 35.0 30.5 38.0 7.5 40.0 74-75 30.64271212874948 35.0 30.0 37.0 4.5 39.0 76-77 30.083849846054562 34.0 29.0 36.5 2.0 39.0 78-79 29.520211221210857 34.0 29.0 36.0 2.0 38.5 80-81 28.941877193218332 34.0 28.5 35.5 2.0 37.5 82-83 28.334122107640802 34.0 27.5 35.0 2.0 37.0 84-85 27.73235005445232 33.0 26.0 35.0 2.0 36.5 86-87 27.092639525659813 33.0 25.5 35.0 2.0 36.0 88-89 26.48370463987523 32.0 24.5 35.0 2.0 35.5 90-91 25.847143606222353 32.0 23.0 34.5 2.0 35.0 92-93 25.149918657649543 32.0 19.5 34.0 2.0 35.0 94-95 24.32457950710569 31.0 12.5 34.0 2.0 35.0 96-97 23.423793645885155 31.0 2.0 34.0 2.0 35.0 98-99 22.19911733465991 30.5 2.0 34.0 2.0 35.0 100-101 19.088313591567285 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05780026041438546 1101 2 0.12124719463214717 1101 3 0.12407050547410137 1101 4 0.19725364948962465 1101 5 -0.057742239646429994 1101 6 -0.009635000761832657 1101 7 0.2518609883539682 1101 8 0.3254264875534858 1101 9 0.2544132367605272 1101 10-11 0.25957189144440207 1101 12-13 0.13337519772480988 1101 14-15 0.2562182454585269 1101 16-17 0.6048405509345258 1101 18-19 0.37491260639034607 1101 20-21 0.5782298927829501 1101 22-23 0.3280571561620391 1101 24-25 0.36073559553172174 1101 26-27 0.21442684641431953 1101 28-29 0.37618288756444684 1101 30-31 0.37395644929448935 1101 32-33 0.7867861693155831 1101 34-35 0.49391614360798286 1101 36-37 0.1397683151373954 1101 38-39 -0.0597665358021402 1101 40-41 0.3917198899989103 1101 42-43 0.4474810608572284 1101 44-45 0.2965565759228781 1101 46-47 0.07957954741796414 1101 48-49 0.27960687057957756 1101 50-51 0.09202506560551882 1101 52-53 0.40731666100192854 1101 54-55 0.30859093172526286 1101 56-57 0.6973136101551987 1101 58-59 1.0408861728636296 1101 60-61 0.8071290821902153 1101 62-63 1.150633861285037 1101 64-65 0.8913006175563893 1101 66-67 1.2866048878131764 1101 68-69 1.289933886735497 1101 70-71 1.297105669379679 1101 72-73 1.474737039913908 1101 74-75 1.435119098650656 1101 76-77 1.349113198556406 1101 78-79 1.447394537519898 1101 80-81 1.2624173754909869 1101 82-83 0.7678415605630384 1101 84-85 1.6588485017881744 1101 86-87 1.2501922932712404 1101 88-89 1.292504150587348 1101 90-91 1.0283953922266775 1101 92-93 1.2798036329629703 1101 94-95 1.4642090649114934 1101 96-97 2.074144876951369 1101 98-99 2.5955251829024526 1101 100-101 2.3486870532281117 1102 1 0.25897163805634804 1102 2 0.1794850352229318 1102 3 -0.03460023554957914 1102 4 0.3255449660162313 1102 5 -0.04847307743609264 1102 6 0.004243277822325808 1102 7 -0.19687850744434598 1102 8 -0.24646044903933273 1102 9 -0.04632524044853881 1102 10-11 0.15395691818236656 1102 12-13 0.068580188812156 1102 14-15 0.34619787367345367 1102 16-17 0.1767402198930128 1102 18-19 0.04401115490065877 1102 20-21 0.2751231955586064 1102 22-23 0.18227156919998322 1102 24-25 0.20315983795596537 1102 26-27 0.1423871214334227 1102 28-29 -0.25526605564919436 1102 30-31 0.054628718202053506 1102 32-33 0.0014029251088132355 1102 34-35 0.19874171019825582 1102 36-37 0.2155895527233369 1102 38-39 0.027692084009416362 1102 40-41 0.14068856837935328 1102 42-43 -0.12109546066047017 1102 44-45 -0.028933620155555673 1102 46-47 -0.4762900731575357 1102 48-49 -0.6202530733980183 1102 50-51 -0.3144175114252974 1102 52-53 -0.9003736878648994 1102 54-55 -0.6613734176508501 1102 56-57 -0.755920410725885 1102 58-59 -0.7749274253029057 1102 60-61 -0.9859317785177026 1102 62-63 -1.156127024887617 1102 64-65 -1.2495957409870257 1102 66-67 -0.8245486645168434 1102 68-69 -0.5122306154290044 1102 70-71 -0.37653833367608414 1102 72-73 -0.16533171486073073 1102 74-75 0.14415907827887153 1102 76-77 0.3293525670310693 1102 78-79 0.34763645246769315 1102 80-81 0.23392234111359755 1102 82-83 0.0543452529490871 1102 84-85 0.09275479157681943 1102 86-87 0.10462315652563348 1102 88-89 0.575226330368352 1102 90-91 0.5486398536476109 1102 92-93 0.712360291990219 1102 94-95 0.9779090903762224 1102 96-97 1.0335540951841153 1102 98-99 0.610842218807722 1102 100-101 0.7674418278652375 1103 1 0.15071547528081908 1103 2 0.27235056280868974 1103 3 0.05490674240790128 1103 4 0.06908872498323859 1103 5 0.11583499612592618 1103 6 0.36957521991180187 1103 7 0.2772965284236548 1103 8 -0.24094981906672075 1103 9 0.40394866068619706 1103 10-11 0.2809133548590381 1103 12-13 0.1685377993508297 1103 14-15 0.1260498366315872 1103 16-17 -0.04826630156257039 1103 18-19 0.12029588165167127 1103 20-21 -0.012391477716469979 1103 22-23 0.14193636638271556 1103 24-25 0.6385811239870094 1103 26-27 0.4220923516408064 1103 28-29 0.2075127365772218 1103 30-31 0.2829285747300361 1103 32-33 0.21829023435623895 1103 34-35 -0.0633718935581058 1103 36-37 0.4681655276809522 1103 38-39 0.4049256825486083 1103 40-41 0.602782607768944 1103 42-43 0.20267850568881585 1103 44-45 0.6548899092562124 1103 46-47 0.7753402907396847 1103 48-49 0.3799262666306831 1103 50-51 0.36635723749866855 1103 52-53 0.3184374507812535 1103 54-55 0.959403939855342 1103 56-57 0.7826213918044473 1103 58-59 1.0767746746057867 1103 60-61 1.13575858277094 1103 62-63 1.6004596452571676 1103 64-65 1.6462077023415276 1103 66-67 1.4904376404264212 1103 68-69 1.030729473262788 1103 70-71 1.0383077599720174 1103 72-73 0.5851900015863798 1103 74-75 0.7990273448759773 1103 76-77 0.7095371242242337 1103 78-79 0.2503852459984124 1103 80-81 0.1334104068498725 1103 82-83 0.7748682736873143 1103 84-85 0.788610406550081 1103 86-87 1.0981017009367449 1103 88-89 0.9890779020391491 1103 90-91 1.0318797127841712 1103 92-93 1.0650243065982785 1103 94-95 1.123500586398137 1103 96-97 1.2803585819455634 1103 98-99 1.5969189111254494 1103 100-101 1.6481644051444881 1104 1 0.16393910149298563 1104 2 0.10793681023742607 1104 3 -0.004673040136911055 1104 4 0.2911721807575489 1104 5 0.12016940693991529 1104 6 0.009011011860081908 1104 7 0.15960625513537963 1104 8 0.1775779505483186 1104 9 0.24093073532621645 1104 10-11 0.5336682769865675 1104 12-13 0.42393744672079237 1104 14-15 0.5410145736283525 1104 16-17 0.543968491267286 1104 18-19 0.7632947062182254 1104 20-21 0.3958145169940792 1104 22-23 0.6939492961505636 1104 24-25 0.4145508565759002 1104 26-27 0.2850808911647533 1104 28-29 0.8009964274382284 1104 30-31 0.39073786065421956 1104 32-33 0.41168576770915166 1104 34-35 0.8508581975379883 1104 36-37 1.1031934442251696 1104 38-39 1.0155633551904018 1104 40-41 1.243871352993743 1104 42-43 1.393723172159575 1104 44-45 1.6232634032321158 1104 46-47 1.557003529058811 1104 48-49 1.4782586204361436 1104 50-51 1.8016349336491189 1104 52-53 1.3522678945073778 1104 54-55 1.6716431405606045 1104 56-57 1.575598176993985 1104 58-59 1.336325071314576 1104 60-61 1.2272954619951477 1104 62-63 1.1782586461387368 1104 64-65 1.5361944787153163 1104 66-67 2.227654801754653 1104 68-69 1.5447244776706697 1104 70-71 0.8918560996722853 1104 72-73 1.3750812739930858 1104 74-75 1.714579798593281 1104 76-77 1.765406944970632 1104 78-79 1.583510429659885 1104 80-81 1.1712814030297132 1104 82-83 0.2303487321063571 1104 84-85 0.8262610089597224 1104 86-87 0.552774048865043 1104 88-89 1.1124984133526965 1104 90-91 1.7921647553936317 1104 92-93 0.8788283030719803 1104 94-95 0.7784374068506779 1104 96-97 1.327816707129223 1104 98-99 1.4203100366029666 1104 100-101 1.8815614077892135 1105 1 0.1890659245747841 1105 2 0.4417985730782661 1105 3 0.38610058066207387 1105 4 0.5678050279357407 1105 5 0.5413805673711138 1105 6 0.6861670042506987 1105 7 0.607688056023143 1105 8 0.5363287431925841 1105 9 0.8076713821239352 1105 10-11 0.6115455756549295 1105 12-13 0.7252611626370893 1105 14-15 0.6700966915738178 1105 16-17 0.8013004506839039 1105 18-19 1.12406674172869 1105 20-21 0.7996271358907165 1105 22-23 0.8570358529038913 1105 24-25 0.9980162972861137 1105 26-27 0.9711937637075536 1105 28-29 1.08836960435643 1105 30-31 1.3495203590689329 1105 32-33 1.3720931868594448 1105 34-35 1.5150940884701356 1105 36-37 1.7610089166411527 1105 38-39 1.73916830129059 1105 40-41 1.9874905666906386 1105 42-43 1.7271489806567288 1105 44-45 2.026600435572007 1105 46-47 2.2224680185959116 1105 48-49 2.0908537377474943 1105 50-51 1.9417744390391007 1105 52-53 2.0836011221548034 1105 54-55 2.562099703655086 1105 56-57 1.8529840362203558 1105 58-59 2.191826022487689 1105 60-61 2.693845874170165 1105 62-63 3.0117240868489503 1105 64-65 2.9262943518922313 1105 66-67 2.9557778201440073 1105 68-69 2.2317885233269763 1105 70-71 2.2084778498308104 1105 72-73 2.5474814008161637 1105 74-75 2.753696792886249 1105 76-77 2.6954164567017678 1105 78-79 3.0181965425324258 1105 80-81 3.174729405566172 1105 82-83 3.6126410593099045 1105 84-85 3.226110406550081 1105 86-87 3.5365644737223683 1105 88-89 3.8744277094845927 1105 90-91 4.066459302001114 1105 92-93 3.4495718033890377 1105 94-95 3.1707566839591124 1105 96-97 2.7884619195578857 1105 98-99 3.0765402204964367 1105 100-101 3.7174840457093126 1106 1 -0.2090878901946951 1106 2 -0.29259555176873775 1106 3 0.21055998498683692 1106 4 0.6260465620034665 1106 5 0.5841182254698438 1106 6 0.6675521531695026 1106 7 -0.14991088318534196 1106 8 0.3573201752611297 1106 9 0.4781120185988428 1106 10-11 0.05220755035912816 1106 12-13 0.34539070160078467 1106 14-15 0.06424703837104317 1106 16-17 0.40954708471083023 1106 18-19 0.49113630517218354 1106 20-21 -0.24369701751605533 1106 22-23 0.38730411075783167 1106 24-25 0.5886425722759157 1106 26-27 0.11409462050070118 1106 28-29 0.35813194625769285 1106 30-31 0.5838124847319293 1106 32-33 0.7410497352070564 1106 34-35 0.9243148147043314 1106 36-37 0.8964128334098191 1106 38-39 0.9463796435998546 1106 40-41 0.7491949730553884 1106 42-43 0.5845796212116028 1106 44-45 1.3012077387135719 1106 46-47 1.2909859704522901 1106 48-49 1.4736821751199969 1106 50-51 1.3490571807771659 1106 52-53 1.4635735823751261 1106 54-55 1.5578157379268163 1106 56-57 1.3012449501330465 1106 58-59 0.9469769812800166 1106 60-61 0.7682032793109244 1106 62-63 0.41048989672247416 1106 64-65 0.08875355221862691 1106 66-67 0.9488418756315582 1106 68-69 1.1942528236125725 1106 70-71 1.6502618155590802 1106 72-73 1.2876384795595293 1106 74-75 1.1096484452730273 1106 76-77 1.9339415485010356 1106 78-79 1.8203181255597656 1106 80-81 1.6953077409394908 1106 82-83 1.4266455472740027 1106 84-85 2.15779645306171 1106 86-87 1.489007354179325 1106 88-89 1.5856935193581094 1106 90-91 2.2537553036331026 1106 92-93 2.2115312077138007 1106 94-95 2.115039629695545 1106 96-97 2.2639566156003177 1106 98-99 1.502335814131687 1106 100-101 0.510425702176061 1107 1 0.3891215213131112 1107 2 -7.514639862264971E-4 1107 3 0.09570028414613674 1107 4 0.3118747091811045 1107 5 0.5108023612925336 1107 6 0.6047177826050287 1107 7 0.642806662398236 1107 8 0.5391085801088167 1107 9 1.0424749403151878 1107 10-11 0.25957189144440207 1107 12-13 0.4688212071144804 1107 14-15 0.3638976552506179 1107 16-17 0.45229192585069455 1107 18-19 0.2708884615211318 1107 20-21 0.31588918185069303 1107 22-23 0.6526715089454029 1107 24-25 0.6391393514002743 1107 26-27 0.5501411321286085 1107 28-29 0.46313795128008195 1107 30-31 0.435324658550023 1107 32-33 0.5342040096911731 1107 34-35 0.9761763716428575 1107 36-37 1.2282995022601284 1107 38-39 0.8272891315352169 1107 40-41 1.0420217008641046 1107 42-43 1.0523435692408611 1107 44-45 0.7974955430590285 1107 46-47 0.8822623106641103 1107 48-49 1.0802440268505293 1107 50-51 0.9134201024935109 1107 52-53 1.1017163927255993 1107 54-55 1.444975908251081 1107 56-57 1.0750466752122065 1107 58-59 1.2814294324209783 1107 60-61 1.469235051338437 1107 62-63 1.7725654508222632 1107 64-65 1.4728566202391562 1107 66-67 1.193546537712578 1107 68-69 1.2627373743277133 1107 70-71 1.407467842417912 1107 72-73 1.3715177240185348 1107 74-75 1.3286133977787635 1107 76-77 1.8009575982881287 1107 78-79 1.2589304194783146 1107 80-81 0.9633228080865592 1107 82-83 1.206506885848082 1107 84-85 1.3326244910571212 1107 86-87 1.3605544663094697 1107 88-89 1.7153747072607217 1107 90-91 1.100326981763903 1107 92-93 1.005122211098449 1107 94-95 2.0607214726915046 1107 96-97 2.6982562116260844 1107 98-99 3.2095090863229743 1107 100-101 3.3687071739524583 1108 1 0.10654416742600858 1108 2 -0.18107751477917589 1108 3 0.2042729801647667 1108 4 0.06894468810979504 1108 5 -0.20944827639183927 1108 6 0.2695176051624202 1108 7 0.5168778612448577 1108 8 0.2527605707930789 1108 9 0.7474766038396439 1108 10-11 0.48594984420031295 1108 12-13 0.5261573499557883 1108 14-15 0.6822962357097495 1108 16-17 0.9458041804808346 1108 18-19 0.4990408403610971 1108 20-21 0.36870608325914134 1108 22-23 0.6223953638860777 1108 24-25 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2.7359319501569352 1108 92-93 3.0871339666712565 1108 94-95 3.821839361124468 1108 96-97 3.036312106047731 1108 98-99 3.176571302137546 1108 100-101 2.9669660183574678 1109 1 0.14547271257281835 1109 2 0.16339227696414582 1109 3 0.3153922678239027 1109 4 0.39921181593376787 1109 5 0.2886752058364337 1109 6 0.21844688619855646 1109 7 0.10713468661832337 1109 8 0.4875092645895336 1109 9 0.5370122576773042 1109 10-11 0.3133102092014113 1109 12-13 0.47652161517029157 1109 14-15 0.6241977737184286 1109 16-17 0.9436701014463225 1109 18-19 0.5756469188069033 1109 20-21 0.39207056924045247 1109 22-23 0.9379814997312437 1109 24-25 0.9482745363416996 1109 26-27 0.7954682349323505 1109 28-29 0.887219825704193 1109 30-31 0.5068002410167907 1109 32-33 0.8332036147984496 1109 34-35 0.6191275365763858 1109 36-37 0.4371648438423321 1109 38-39 0.529441296863638 1109 40-41 0.9696905175022508 1109 42-43 1.286021336780685 1109 44-45 0.26820766626556036 1109 46-47 0.5155386039377632 1109 48-49 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2316 66-67 0.2151763163846141 2316 68-69 0.0335846803862907 2316 70-71 -0.24019591792190553 2316 72-73 -0.540342325165458 2316 74-75 -0.2617062981747402 2316 76-77 -0.1691407696975631 2316 78-79 -0.012922922797560688 2316 80-81 -0.6558365927629843 2316 82-83 -0.3488251061036287 2316 84-85 -0.510992768053093 2316 86-87 -0.27123627815733187 2316 88-89 -0.49717838909518974 2316 90-91 -0.2811284172971291 2316 92-93 0.24170839486773232 2316 94-95 0.32055827126070113 2316 96-97 1.0685893213958124 2316 98-99 1.6010807384580055 2316 100-101 0.8177346722757299 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 796.0 3 153.0 4 116.0 5 137.0 6 141.0 7 198.0 8 206.0 9 258.0 10 320.0 11 384.0 12 562.0 13 685.0 14 821.0 15 1001.0 16 1219.0 17 1660.0 18 1339.0 19 986.0 20 1071.0 21 1097.0 22 1284.0 23 1377.0 24 1630.0 25 1836.0 26 2198.0 27 2524.0 28 2887.0 29 3473.0 30 4267.0 31 5171.0 32 6564.0 33 8443.0 34 11203.0 35 15455.0 36 21075.0 37 26250.0 38 18191.0 39 1775.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.9300906731385 20.096403411197628 15.877574409276301 22.09593150638757 2 14.638765332255021 20.528373096104595 41.48065777058903 23.352203801051356 3 18.825558984864617 25.193740986832353 30.332088898472986 25.648611129830044 4 12.164138367916328 17.462446171160547 39.12269942784745 31.250716033075676 5 13.122885525198475 37.95136876440539 35.008289415158174 13.917456295237967 6 29.862098235638886 37.97519519802747 18.203437102109284 13.959269464224361 7 27.027081649151175 33.77391538668822 21.368229587712207 17.8307733764484 8 25.549432040208586 35.60696912939781 20.317195100588844 18.526403729804752 9 25.300173838047623 17.65332111525867 21.291118088590025 35.755386958103685 10-11 23.907806651370738 27.847687585702687 27.895192563617925 20.349313199308654 12-13 25.187056782387522 24.98660872201728 28.291475061903075 21.53485943369212 14-15 21.907989140320268 26.54760541367161 26.944064565242286 24.600340880765835 16-17 21.06628222660855 29.944220262595913 27.43615308439043 21.553344426405104 18-19 20.980664890661153 28.27182988067533 30.027178946588485 20.72032628207503 20-21 21.623806396200678 27.234881018541905 30.446454133616264 20.694858451641153 22-23 21.352886098630545 27.088708210679474 30.367523728048607 21.190881962641374 24-25 21.157343787891037 27.49171449357368 30.37008595370894 20.98085576482634 26-27 20.865582162945 27.555824997473984 30.353305715536692 21.22528712404432 28-29 20.87607075126907 27.94749234516002 30.148785162579035 21.027651740991878 30-31 21.148203174218153 27.724043135946957 29.976181572555245 21.151572117279645 32-33 20.810847229240355 27.75206333164898 30.00707427993936 21.430015159171298 34-35 21.44726780282231 27.77506408982345 29.821222237568595 20.956445869785647 36-37 21.473777101149558 27.527054837199433 29.886122123160558 21.11304593849045 38-39 20.954449776879684 27.89424938957649 29.806180011787486 21.345120821756336 40-41 21.47398980203555 27.713338856669427 29.936144846121206 20.876526495173817 42-43 21.24570993610794 27.734273695130124 29.55201460389954 21.4680017648624 44-45 21.20949814752442 28.12697878073425 29.4493095318289 21.21421353991243 46-47 21.70970860378401 28.186040871595015 28.96817243272557 21.136078091895406 48-49 21.43367243818635 28.265849221855422 28.89139661793438 21.40908172202385 50-51 21.481873279721857 28.493027925357197 28.7093155272263 21.31578326769465 52-53 21.712968890026886 28.43291175063507 28.64448921561361 21.209630143724436 54-55 21.315457041316467 28.528069259402738 28.690438092671506 21.46603560660929 56-57 21.376655394937153 28.570803077047437 28.781305993775852 21.271235534239562 58-59 21.330985038036324 28.442548147349918 28.78165905042212 21.44480776419164 60-61 21.119256971782843 28.53977309082092 28.91593449336077 21.425035444035466 62-63 21.26273035887488 28.407560081905377 29.00972626360599 21.31998329561375 64-65 21.115837046167112 28.64219732406556 28.835163650936373 21.406801978830952 66-67 21.150122084701525 28.60149869495664 28.47554096152227 21.77283825881957 68-69 21.2824571447816 28.459232815815177 28.67881318829354 21.57949685110969 70-71 21.412116558218486 28.374355036441283 28.564307750340163 21.649220655000068 72-73 21.317741995237725 28.402213413176074 28.425789025215803 21.8542555663704 74-75 21.196373360636414 28.72616313141179 28.308870582054062 21.768592925897735 76-77 21.41613308498348 28.38901557126642 28.346912860862332 21.847938482887773 78-79 21.293049731386517 28.568012260226677 28.284747132824734 21.854190875562068 80-81 21.47050403327664 28.625654670685908 28.132567656321044 21.77127363971641 82-83 21.348655184259627 28.68030904155356 28.375847877191685 21.59518789699513 84-85 21.32353188781094 28.680340648498298 28.29174004842354 21.70438741526722 86-87 21.380534311298803 28.774209191663047 28.120885153042842 21.724371343995312 88-89 21.37644186242317 28.790435295108193 27.98922286772754 21.843899974741095 90-91 21.27074149038996 28.772534848398102 28.36199899639308 21.594724664818862 92-93 21.570291634249763 28.43492875017261 28.303577142125807 21.69120247345182 94-95 21.337374757935507 28.326345036625412 28.30411720131346 22.03216300412562 96-97 21.353415872930334 28.29933851563447 28.343796731647515 22.00344887978768 98-99 21.39597387769399 28.275813290851463 27.93193920063588 22.396273630818662 100-101 21.73962827131681 27.594742319077785 28.421180250356137 22.244449159249267 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 212.0 1 119.5 2 30.0 3 32.0 4 35.5 5 40.5 6 49.5 7 78.0 8 132.0 9 174.0 10 155.0 11 140.0 12 151.0 13 160.5 14 180.0 15 192.0 16 197.5 17 206.0 18 240.5 19 275.0 20 309.0 21 362.5 22 427.0 23 504.5 24 629.0 25 793.0 26 1056.5 27 1344.5 28 1575.0 29 1870.5 30 2178.0 31 2579.5 32 3058.0 33 3408.0 34 3778.5 35 4057.0 36 4421.5 37 4796.0 38 5003.5 39 5179.0 40 5259.0 41 5344.5 42 5424.5 43 5500.5 44 5496.0 45 5594.5 46 5643.5 47 5572.5 48 5536.5 49 5387.5 50 5073.0 51 4665.0 52 4402.0 53 4251.0 54 4033.0 55 3686.0 56 3238.0 57 2790.5 58 2526.0 59 2214.5 60 1706.5 61 1351.5 62 1112.0 63 815.0 64 549.5 65 407.0 66 300.0 67 220.5 68 170.0 69 130.0 70 100.5 71 68.5 72 43.0 73 27.0 74 23.0 75 20.5 76 15.5 77 9.0 78 6.0 79 4.5 80 2.5 81 2.0 82 2.0 83 1.5 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28167309786627587 2 0.251421810505936 3 0.2420102988827191 4 0.24671605469432753 5 0.2500773088454764 6 0.21175901152237922 7 0.21108676069214946 8 0.2204982723153663 9 0.2285652822781236 10-11 0.2342794143350767 12-13 0.22621240437231938 14-15 0.21175901152237922 16-17 0.2104145098619197 18-19 0.19730561867243906 20-21 0.20604487946542616 22-23 0.20234749989916237 24-25 0.20301975072939213 26-27 0.2010029982387028 28-29 0.21411188942818343 30-31 0.2282291568630087 32-33 0.22117052314559607 34-35 0.22150664856071095 36-37 0.20537262863519634 38-39 0.1959611170119795 40-41 0.19696949325732419 42-43 0.2020113744840475 44-45 0.2043642523898517 46-47 0.21075063527703458 48-49 0.21780926899444722 50-51 0.22890140769323852 52-53 0.2319265364292725 54-55 0.21948989607002167 56-57 0.2016752490689326 58-59 0.18654960538876264 60-61 0.18856635787945197 62-63 0.19495274076663485 64-65 0.18991085953991152 66-67 0.1959611170119795 68-69 0.19428048993640507 70-71 0.19898624574801352 72-73 0.19865012033289858 74-75 0.19965849657824328 76-77 0.20671713029565592 78-79 0.20604487946542616 80-81 0.20268362531427725 82-83 0.1983139949177837 84-85 0.18318835123761376 86-87 0.18991085953991152 88-89 0.1959611170119795 90-91 0.19461661535151994 92-93 0.19999462199335816 94-95 0.1959611170119795 96-97 0.2016752490689326 98-99 0.20066687282358792 100-101 0.19192761203060085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 148754.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.72366626509012 #Duplication Level Percentage of deduplicated Percentage of total 1 90.80418712289163 74.20851083903989 2 6.254710869276946 10.223158073308413 3 1.2909024561165938 3.164918445133729 4 0.5615397272359387 1.835643410528783 5 0.295202805415644 1.2062527775153213 6 0.17146551973892274 0.8407674546668366 7 0.1295841105148737 0.7413062020683264 8 0.09369737067286077 0.6125834120628252 9 0.074758203024144 0.5498562991070696 >10 0.29703889658166227 4.037162104030901 >50 0.019935495207993946 1.0467621290057547 >100 0.005980648562398184 1.0492059316415658 >500 9.967747603996974E-4 0.48387292189059794 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 594 0.39931699315648655 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 432 0.2904123586592629 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 283 0.19024698495502643 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 220 0.14789518265055057 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 6.722508302297753E-4 0.0 0.0 0.0 0.0 16-17 6.722508302297753E-4 0.0 0.0 0.0 0.0 18-19 6.722508302297753E-4 0.0 0.0 0.0 0.0 20-21 6.722508302297753E-4 0.0 0.0 0.0 0.0 22-23 6.722508302297753E-4 0.0 0.0 0.0 0.0 24-25 6.722508302297753E-4 0.0 0.0 0.0 0.0 26-27 6.722508302297753E-4 0.0 0.0 6.722508302297753E-4 0.0 28-29 6.722508302297753E-4 0.0 0.0 0.0016806270755744383 0.0 30-31 6.722508302297753E-4 0.0 0.0 0.00436963039649354 0.0 32-33 6.722508302297753E-4 0.0 0.0 0.013445016604595506 0.0 34-35 6.722508302297753E-4 0.0 0.0 0.017814647001089045 0.0 36-37 6.722508302297753E-4 0.0 0.0 0.02016752490689326 0.0 38-39 6.722508302297753E-4 0.0 0.0 0.024873280718501686 0.0 40-41 6.722508302297753E-4 0.0 0.0 0.03226803985102922 0.0 42-43 0.001008376245344663 0.0 0.0 0.0490743106067736 0.0 44-45 0.0013445016604595507 0.0 0.0 0.07428371674039017 0.0 46-47 0.0013445016604595507 0.0 0.0 0.10050149911935141 0.0 48-49 0.0013445016604595507 0.0 0.0 0.15999569759468651 0.0 50-51 0.0013445016604595507 0.0 0.0 0.2238595264665152 0.0 52-53 0.0013445016604595507 0.0 0.0 0.27629509122443763 0.0 54-55 0.0013445016604595507 0.0 0.0 0.397972491496027 0.0 56-57 0.0013445016604595507 0.0 0.0 0.5004907431060677 0.0 58-59 0.0013445016604595507 0.0 0.0 0.5888917272812833 0.0 60-61 0.0013445016604595507 0.0 0.0 0.6853597214192559 0.0 62-63 0.0013445016604595507 0.0 0.0 0.8235072670314748 0.0 64-65 0.0013445016604595507 0.0 0.0 0.915941756188069 0.0 66-67 0.0013445016604595507 0.0 0.0 1.038627532705003 0.0 68-69 0.0013445016604595507 0.0 0.0 1.2510587950576117 0.0 70-71 0.0013445016604595507 0.0 0.0 1.412062868897643 0.0 72-73 0.0016806270755744383 0.0 0.0 1.669198811460532 0.0 74-75 0.002016752490689326 0.0 0.0 1.9938959624615136 0.0 76-77 0.002016752490689326 0.0 0.0 2.252376406684862 0.0 78-79 0.002016752490689326 0.0 0.0 2.51354585422913 0.0 80-81 0.002016752490689326 0.0 0.0 2.8610995334579234 0.0 82-83 0.002016752490689326 0.0 0.0 3.2466353845947 0.0 84-85 0.002016752490689326 0.0 0.0 3.605953453352515 0.0 86-87 0.002016752490689326 0.0 0.0 4.057033760436695 0.0 88-89 0.002016752490689326 0.0 0.0 4.614665824112293 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTCT 25 0.0050043846 57.02386 9 GGTATCA 405 0.0 51.643948 1 TATAGGG 40 5.361851E-4 47.519886 2 GTAAGGT 55 4.9301525E-5 43.199898 4 GTATCAA 635 0.0 36.68125 1 TAAGGTG 85 1.6180373E-5 33.54345 5 GTAGTAG 60 0.003925413 31.690605 1 TAGAGGT 75 3.0581182E-4 31.679922 4 AGGTAAG 75 3.0581182E-4 31.679922 2 TATAAGC 60 0.0039319005 31.679922 2 TATGAGT 95 3.4607023E-5 30.012558 4 ATCAACG 780 0.0 28.63378 3 TCAACGC 790 0.0 28.271322 4 TATCAAC 820 0.0 27.237007 2 CAACGCA 820 0.0 27.237007 5 GTCACGG 35 0.0083646225 27.15422 46-47 GAGTACT 555 0.0 26.961658 12-13 AACGCAG 840 0.0 26.588507 6 GGTAAGG 90 8.888966E-4 26.399937 3 GTAGCCC 90 8.888966E-4 26.399937 3 >>END_MODULE