FastQCFastQC Report
Thu 26 May 2016
SRR937227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148754
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.5546069349395647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.45242480874463875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.28705110450811405No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.1115936378181427No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG1620.10890463449722361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC502.815105E-547.4998363
ACAGTCC608.256141E-539.5831958
GTATCAA7350.039.4348831
GGTATCA4300.037.5706371
GTCTAGA651.3200444E-436.5506171
ATAGGAC550.002572487334.545333
CTAGACT702.0448225E-433.928454
TAAGGTG902.3990287E-531.6665535
ATCAACG9200.030.9781513
TCAACGC9250.030.81074
TACAGGC1401.6849299E-830.5356064
CAACGCA9450.030.1586235
ACAGTCT804.475156E-429.6873938
GAACAGT804.475156E-429.6873936
TATCAAC9650.029.5335732
AACGCAG9750.029.2306676
TATGCAA650.005828044429.2306655
TACTATA650.005828044429.2306652
GTCATAT700.00835493827.1518881
ATGGGAC700.00836865927.1427617
Produced by FastQC (version 0.11.5)