FastQCFastQC Report
Thu 26 May 2016
SRR937226_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937226_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139933
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6010.42949125652991077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4120.29442661845311685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.19152022753746437No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1760.1257744777857975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTGT502.8129167E-547.503581
GTCTTAT405.3698634E-447.5035741
GGTATCA3900.046.2855381
GTATCAA5900.034.621251
TAAGGTG550.002566455234.5604175
GTATAGG550.002570964434.5480581
GTGTAAG600.00393774231.669051
ACTGTAC953.4674013E-530.002268
TATAGGA1004.919946E-528.5021462
TAGGGCA856.362706E-427.953284
TCTAAGG856.376041E-427.9432813
TCAACGC7150.027.9141834
ATCAACG7200.027.710423
ACATGGC1056.859738E-527.1449013
CAATATC350.00837782827.144962-63
CAACGCA7450.026.7901215
AACGCAG7450.026.7805396
TATCAAC7650.026.0803952
TGCACTG1109.390671E-525.9203135
TAATACA1000.001642267523.7602864
Produced by FastQC (version 0.11.5)