##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937226_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 139933 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.791757483938742 33.0 31.0 34.0 30.0 34.0 2 31.907977389179106 33.0 31.0 34.0 30.0 34.0 3 31.94908992160534 34.0 31.0 34.0 30.0 34.0 4 35.53773591647431 37.0 35.0 37.0 33.0 37.0 5 35.54214516947396 37.0 35.0 37.0 33.0 37.0 6 35.58656642821922 37.0 35.0 37.0 33.0 37.0 7 35.535649203547415 37.0 35.0 37.0 33.0 37.0 8 35.532118942636835 37.0 35.0 37.0 33.0 37.0 9 37.16545775478264 39.0 37.0 39.0 34.0 39.0 10-11 37.06763236691845 39.0 37.0 39.0 33.5 39.0 12-13 36.94001772276732 39.0 37.0 39.0 33.0 39.0 14-15 38.130312363774095 40.0 38.0 41.0 33.0 41.0 16-17 38.09757526816405 40.0 38.0 41.0 33.0 41.0 18-19 38.08258595184839 40.0 38.0 41.0 33.5 41.0 20-21 38.025823072470395 40.0 38.0 41.0 33.0 41.0 22-23 37.88297256544203 40.0 38.0 41.0 33.0 41.0 24-25 37.75390008075293 40.0 38.0 41.0 33.0 41.0 26-27 37.586530696833485 40.0 37.0 41.0 32.5 41.0 28-29 37.63520041734259 40.0 37.0 41.0 33.0 41.0 30-31 37.47096467595206 40.0 37.0 41.0 32.0 41.0 32-33 37.29028892398505 40.0 37.0 41.0 32.0 41.0 34-35 37.16962760749787 40.0 36.5 41.0 31.0 41.0 36-37 37.16041962939407 40.0 37.0 41.0 31.0 41.0 38-39 37.07252042048695 40.0 37.0 41.0 31.0 41.0 40-41 36.90887067382248 40.0 36.5 41.0 31.0 41.0 42-43 36.6706745370999 40.0 36.0 41.0 30.0 41.0 44-45 36.470246475098804 39.5 36.0 41.0 30.0 41.0 46-47 36.13119850214031 39.0 36.0 41.0 29.5 41.0 48-49 35.82919325677288 39.0 35.0 41.0 28.0 41.0 50-51 34.9686171239093 38.5 34.0 40.0 26.5 40.5 52-53 35.04267399398283 38.5 35.0 40.0 27.0 40.5 54-55 35.38287251756198 39.0 35.0 40.0 27.0 41.0 56-57 35.187357521099386 39.0 35.0 40.0 26.0 41.0 58-59 34.985046415070066 38.5 35.0 40.0 26.0 41.0 60-61 34.71383090479015 38.0 34.0 40.0 26.0 41.0 62-63 34.4037110617224 38.0 34.0 40.0 25.0 41.0 64-65 34.05937484367519 37.5 34.0 40.0 23.5 41.0 66-67 33.705927122265656 37.0 33.5 40.0 22.5 41.0 68-69 33.25147034652298 36.0 33.0 39.0 22.0 41.0 70-71 32.819413576497325 36.0 32.5 39.0 20.5 40.5 72-73 32.42162677852973 35.5 32.0 38.5 19.5 40.0 74-75 31.959291232232566 35.0 32.0 37.5 18.0 40.0 76-77 31.537950304788723 35.0 31.5 37.0 15.0 39.0 78-79 31.051781924206587 35.0 31.0 36.5 9.5 39.0 80-81 30.55959280512817 34.5 31.0 36.0 7.0 38.5 82-83 30.08733107987394 34.0 30.0 35.5 4.0 37.0 84-85 29.640474369877012 34.0 30.0 35.0 2.0 37.0 86-87 29.223957179507337 34.0 29.0 35.0 2.0 36.0 88-89 28.81458626628458 34.0 29.0 35.0 2.0 36.0 90-91 28.3876355112804 34.0 29.0 35.0 2.0 35.5 92-93 27.972540430062956 34.0 28.5 35.0 2.0 35.0 94-95 27.45522500053597 33.5 26.5 35.0 2.0 35.0 96-97 26.844446985342984 33.0 25.5 35.0 2.0 35.0 98-99 26.120714913565777 33.0 24.5 35.0 2.0 35.0 100-101 24.22900245117306 30.5 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2049780056168906 1101 2 0.2615164267559855 1101 3 0.1515294289072493 1101 4 -0.08890720195530122 1101 5 0.10579432589759108 1101 6 0.1694454712508886 1101 7 0.18264605078158525 1101 8 0.21490268906831744 1101 9 0.24758929957533837 1101 10-11 0.12894028030301996 1101 12-13 0.057295459856995024 1101 14-15 0.4123533799825978 1101 16-17 -0.128055020186693 1101 18-19 0.22106218146675616 1101 20-21 0.3172504622323089 1101 22-23 0.47024403632986633 1101 24-25 0.3277337951347761 1101 26-27 0.49129780289007385 1101 28-29 0.14545668764239394 1101 30-31 -0.03924927745060103 1101 32-33 0.20872543340979632 1101 34-35 0.08869493631021186 1101 36-37 0.051488440813237446 1101 38-39 -0.11397220271285136 1101 40-41 -0.11560669595306194 1101 42-43 -0.129889215859329 1101 44-45 -0.2059268359708213 1101 46-47 0.05682645888783355 1101 48-49 -0.02420537406712242 1101 50-51 -0.3773555125485899 1101 52-53 -0.46642595735374215 1101 54-55 0.17330795161110046 1101 56-57 -0.027565605261749226 1101 58-59 -0.46941312932515444 1101 60-61 -0.5230170501069509 1101 62-63 -0.41388976713704295 1101 64-65 -0.2664728159304772 1101 66-67 -0.031631219760448914 1101 68-69 -0.23983904915520782 1101 70-71 -0.4734623208575286 1101 72-73 -0.5055556587599952 1101 74-75 -0.5189428913665672 1101 76-77 -0.38279051916871865 1101 78-79 -0.3019134616263308 1101 80-81 0.27460306856376704 1101 82-83 0.13324971588357926 1101 84-85 0.08029360992873791 1101 86-87 0.180944579944331 1101 88-89 -0.4504455214935845 1101 90-91 0.05906935472380681 1101 92-93 0.675675253706526 1101 94-95 0.713033085039509 1101 96-97 0.24615817910391868 1101 98-99 -0.24242000581383039 1101 100-101 0.003230183312691537 1102 1 0.5757097129339606 1102 2 0.5646305382542458 1102 3 0.6061025996389553 1102 4 0.5987670140725783 1102 5 0.69468805412059 1102 6 0.5170064468606412 1102 7 0.5716268870185175 1102 8 0.4003338737373099 1102 9 0.6941703100282979 1102 10-11 0.5371828761218325 1102 12-13 0.5513394494040327 1102 14-15 0.4004086935714426 1102 16-17 0.8486653630885641 1102 18-19 1.0926431919197128 1102 20-21 0.8106629186782968 1102 22-23 1.1039221722183683 1102 24-25 0.6634589693508062 1102 26-27 0.7650129596844977 1102 28-29 1.0488756771894359 1102 30-31 0.9224667504239648 1102 32-33 1.167675781841858 1102 34-35 1.0797010338711814 1102 36-37 0.9572484582348437 1102 38-39 1.4367028843951672 1102 40-41 1.1809852030016472 1102 42-43 0.9593638329211629 1102 44-45 1.4295587912068868 1102 46-47 1.9541043334522854 1102 48-49 1.9317833018910662 1102 50-51 1.5187137383224965 1102 52-53 1.5518449485696024 1102 54-55 1.4298192756529104 1102 56-57 1.2110123564107198 1102 58-59 1.234180076249757 1102 60-61 1.1052712774191846 1102 62-63 0.7853153722357789 1102 64-65 0.8517327589824149 1102 66-67 0.9736279265810097 1102 68-69 0.751101717395315 1102 70-71 0.43511781851529463 1102 72-73 0.6687583656302465 1102 74-75 0.8888206103755927 1102 76-77 0.8639320418068941 1102 78-79 0.8920651969102558 1102 80-81 0.8274123020132471 1102 82-83 0.9898154824340999 1102 84-85 0.7828306308346598 1102 86-87 1.0177259388293471 1102 88-89 1.2213859244994474 1102 90-91 1.0506634313788545 1102 92-93 1.223922204926037 1102 94-95 1.156031778419301 1102 96-97 1.7777021686509578 1102 98-99 2.274261178855159 1102 100-101 2.956812500385862 1103 1 0.27640657704546 1103 2 0.30552152879680605 1103 3 0.31351922482561534 1103 4 -0.039162303996114645 1103 5 0.07390657079555041 1103 6 -0.08565656956543677 1103 7 -0.34158864309596737 1103 8 -0.1926228211357639 1103 9 0.008431136310029785 1103 10-11 0.02402956601730466 1103 12-13 0.09779290883658831 1103 14-15 -0.07074610981332086 1103 16-17 -0.25241726508465234 1103 18-19 0.10690401820144757 1103 20-21 -0.09059392552279633 1103 22-23 -0.2520136167313538 1103 24-25 0.18583328493069473 1103 26-27 0.13861923146149735 1103 28-29 0.14736995294852306 1103 30-31 0.030903783773887028 1103 32-33 0.0996693109608131 1103 34-35 0.326577589371432 1103 36-37 0.5294858897928236 1103 38-39 -0.039354855774071495 1103 40-41 0.1844570795571414 1103 42-43 0.4947919065896471 1103 44-45 0.13112673545775522 1103 46-47 0.2207295201123216 1103 48-49 0.6090854422594063 1103 50-51 0.6242388752065082 1103 52-53 0.49084445080952577 1103 54-55 0.6127212169172225 1103 56-57 0.6347430682076336 1103 58-59 0.6779082992462762 1103 60-61 0.46486560295426926 1103 62-63 0.1540311512302992 1103 64-65 0.7080169799878959 1103 66-67 0.8079733720762832 1103 68-69 1.160671154926419 1103 70-71 1.2395478832241054 1103 72-73 0.9367275045053134 1103 74-75 0.9360953739395619 1103 76-77 0.7833446849129153 1103 78-79 0.6869258240879539 1103 80-81 1.3300877624413197 1103 82-83 1.1488747158835793 1103 84-85 0.780229834418531 1103 86-87 1.524694579944331 1103 88-89 1.462500907077846 1103 90-91 1.2312632322748236 1103 92-93 1.011453314931014 1103 94-95 0.9218978809578751 1103 96-97 1.0175229750222883 1103 98-99 0.7563044839820883 1103 100-101 1.055207224129017 1104 1 0.025083825722710174 1104 2 0.0275547865443464 1104 3 0.11019345007126802 1104 4 0.09462454407644572 1104 5 0.08165411425738256 1104 6 -0.21547516366974406 1104 7 -0.1649068592713263 1104 8 0.1443008372164698 1104 9 0.05661707735311694 1104 10-11 0.21781263480037438 1104 12-13 -0.10581631262977709 1104 14-15 0.16375880326301484 1104 16-17 0.550020376638706 1104 18-19 0.41170371585828747 1104 20-21 0.051790806147650414 1104 22-23 0.6093809410917714 1104 24-25 0.18413260465858627 1104 26-27 -0.11733314949088225 1104 28-29 0.09701092044662829 1104 30-31 0.2990443733430581 1104 32-33 0.558758502192866 1104 34-35 0.3620084283737057 1104 36-37 0.7332840757338701 1104 38-39 0.42323017823952824 1104 40-41 0.7085500500786921 1104 42-43 0.4285598582147472 1104 44-45 0.6880050423360657 1104 46-47 1.1079177287290989 1104 48-49 1.0864097052979602 1104 50-51 0.932250968932891 1104 52-53 0.9940568468790687 1104 54-55 1.2720182690714168 1104 56-57 0.816432410611263 1104 58-59 1.0390194103573833 1104 60-61 1.3666985583586566 1104 62-63 0.9327537513814761 1104 64-65 0.7381568136991561 1104 66-67 0.7796287008744756 1104 68-69 1.1305313212151589 1104 70-71 1.3846725997773888 1104 72-73 1.3832505581712198 1104 74-75 1.5603395160408375 1104 76-77 1.4787339517307494 1104 78-79 1.4177459299080617 1104 80-81 1.2903107405214378 1104 82-83 1.438557255566117 1104 84-85 1.217777075537203 1104 86-87 1.4506205058702548 1104 88-89 1.274670219247156 1104 90-91 1.530299513453965 1104 92-93 1.2081653330716051 1104 94-95 1.1967467093781323 1104 96-97 1.0311284171991595 1104 98-99 0.9933604174665938 1104 100-101 1.4806607388682487 1105 1 0.2958870965259841 1105 2 0.1751257341152943 1105 3 0.15569609557391928 1105 4 0.3459953954472965 1105 5 0.369484802088067 1105 6 0.08005153185695235 1105 7 0.40488631052184587 1105 8 0.2671483600639917 1105 9 0.6320860528220891 1105 10-11 0.6741377911255242 1105 12-13 0.4043327975193378 1105 14-15 0.3803052198094363 1105 16-17 0.7389092655275888 1105 18-19 0.5154074195619884 1105 20-21 0.2360139903708358 1105 22-23 0.393796525507355 1105 24-25 0.3469031674291543 1105 26-27 0.403446071288343 1105 28-29 0.37156599500170273 1105 30-31 0.26296933726801797 1105 32-33 0.20426114769551162 1105 34-35 -0.027024760659486446 1105 36-37 0.23862318107298108 1105 38-39 0.8375158925252464 1105 40-41 0.33567847720711796 1105 42-43 0.2272130568679458 1105 44-45 -0.15610270177167962 1105 46-47 0.35366087447224714 1105 48-49 0.20791042679868355 1105 50-51 0.35293398961591294 1105 52-53 0.3742396270618471 1105 54-55 0.39914669619984977 1105 56-57 0.3932000873789434 1105 58-59 0.6616745330125084 1105 60-61 0.6205706122307078 1105 62-63 0.375707094334814 1105 64-65 0.706795582337925 1105 66-67 0.7286907499365256 1105 68-69 0.21037740106123692 1105 70-71 0.15278226788706206 1105 72-73 0.5935920685127272 1105 74-75 0.5815035372048634 1105 76-77 0.629046602043406 1105 78-79 1.092878205040332 1105 80-81 0.60753055774126 1105 82-83 1.0612123782212421 1105 84-85 1.0859348436949716 1105 86-87 0.8952573505071015 1105 88-89 0.6239727685497058 1105 90-91 0.7802273633818153 1105 92-93 0.9409214658277385 1105 94-95 0.7741396867711074 1105 96-97 0.5852409713117126 1105 98-99 0.9501665742727532 1105 100-101 1.0432870014945088 1106 1 0.16626832819753545 1106 2 0.1995636617790275 1106 3 0.3277391063266073 1106 4 0.5043531667082988 1106 5 0.455506307464411 1106 6 0.4000906325412146 1106 7 0.19491563603504147 1106 8 0.2827886337687815 1106 9 0.06962270971358464 1106 10-11 -0.37170776117164195 1106 12-13 0.3371773722993936 1106 14-15 -0.12634189651510042 1106 16-17 0.21310281391468777 1106 18-19 0.6745472045082295 1106 20-21 0.38713813504336514 1106 22-23 0.25577111006258946 1106 24-25 0.673687136148601 1106 26-27 0.39357314851218916 1106 28-29 0.6798748904073761 1106 30-31 0.36990970872452067 1106 32-33 0.4881321154374447 1106 34-35 0.9247836459876311 1106 36-37 0.5066583716888147 1106 38-39 0.7353653548908383 1106 40-41 0.5698915759363459 1106 42-43 0.10609868736647599 1106 44-45 0.7373479336144371 1106 46-47 1.2002884404546492 1106 48-49 1.3217911697116804 1106 50-51 1.4134422985113204 1106 52-53 1.3501927062407333 1106 54-55 1.3365856013806905 1106 56-57 1.2848911919732728 1106 58-59 0.825604151780837 1106 60-61 0.8522421664829096 1106 62-63 1.2322075830933699 1106 64-65 1.0822599029635356 1106 66-67 1.823509909271813 1106 68-69 1.5993314577572306 1106 70-71 1.227171319695465 1106 72-73 1.5695451476916205 1106 74-75 1.3621486984951865 1106 76-77 1.682028029218376 1106 78-79 2.0939534738575354 1106 80-81 2.2566019164900304 1106 82-83 2.4703032873121487 1106 84-85 2.4434128495600724 1106 86-87 1.9523443495295822 1106 88-89 1.8707958379534233 1106 90-91 2.2158089860602104 1106 92-93 2.6900905762871723 1106 94-95 2.6373850435648514 1106 96-97 2.08612073670761 1106 98-99 2.453490132435018 1106 100-101 2.4573632478288197 1107 1 0.23702928766817521 1107 2 0.22454298353254742 1107 3 0.2172345571123806 1107 4 0.4337576332095381 1107 5 0.2823053149085837 1107 6 0.5925223943278084 1107 7 0.5171240727596071 1107 8 0.5529292458448793 1107 9 0.8630883838244188 1107 10-11 0.8962823132700493 1107 12-13 0.8324213756079146 1107 14-15 1.0275662920705102 1107 16-17 0.7626272142455406 1107 18-19 0.6859202400748146 1107 20-21 0.9038461582030024 1107 22-23 0.7325494392602749 1107 24-25 0.6003413958673818 1107 26-27 0.36801483585710315 1107 28-29 0.5812396546753575 1107 30-31 0.7842397335384135 1107 32-33 1.0696457630801248 1107 34-35 1.0269962183614965 1107 36-37 0.9097187521685441 1107 38-39 0.768926148935499 1107 40-41 0.8970421135707554 1107 42-43 0.5019216815765688 1107 44-45 0.3902842446152661 1107 46-47 1.189266935078301 1107 48-49 0.9978871167753667 1107 50-51 0.421465458147388 1107 52-53 0.19760792543014105 1107 54-55 0.883587255640407 1107 56-57 0.8335264277052801 1107 58-59 0.9073039036418749 1107 60-61 1.1181230597831586 1107 62-63 0.9114879801157088 1107 64-65 1.1364575869999314 1107 66-67 1.0641219853677555 1107 68-69 0.8924869581707924 1107 70-71 1.1792391443439385 1107 72-73 0.8746526745733334 1107 74-75 1.0750932807946079 1107 76-77 0.7738601218569237 1107 78-79 1.074288461450589 1107 80-81 0.9988475740582743 1107 82-83 1.1529955950044553 1107 84-85 1.0584872912474204 1107 86-87 0.8969473271970756 1107 88-89 1.238599808176744 1107 90-91 1.2703788785333252 1107 92-93 1.8884156383219128 1107 94-95 1.6085802462116696 1107 96-97 1.8530148640856048 1107 98-99 1.5883950125011914 1107 100-101 1.5340795422870492 1108 1 0.28661065867811786 1108 2 0.32082765124578216 1108 3 0.27270289829500527 1108 4 0.022062185799804013 1108 5 0.2728861626322896 1108 6 0.33101009710122753 1108 7 0.4050099963598157 1108 8 0.3158805802247855 1108 9 0.5033290954937044 1108 10-11 0.7442676612553996 1108 12-13 0.5034051537345476 1108 14-15 0.4139477677376959 1108 16-17 0.33686844920105585 1108 18-19 0.5269720454123288 1108 20-21 -0.026818415318714983 1108 22-23 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-2.3796917839520546 2316 90-91 -2.5348536796062078 2316 92-93 -2.1115098238128525 2316 94-95 -2.3949230334544005 2316 96-97 -1.6970000434874422 2316 98-99 -2.5517361741416273 2316 100-101 -3.602026599633046 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 668.0 3 128.0 4 96.0 5 103.0 6 111.0 7 154.0 8 182.0 9 217.0 10 265.0 11 335.0 12 458.0 13 559.0 14 701.0 15 862.0 16 1067.0 17 1316.0 18 1086.0 19 811.0 20 750.0 21 832.0 22 865.0 23 968.0 24 1125.0 25 1163.0 26 1460.0 27 1690.0 28 2010.0 29 2399.0 30 2995.0 31 3595.0 32 4754.0 33 6265.0 34 8642.0 35 12669.0 36 19398.0 37 29120.0 38 26266.0 39 3847.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.08806563720397 20.10533481423095 15.84966500662821 21.95693454193687 2 14.583019908730755 20.720410926518944 41.51532735856491 23.18124180618539 3 18.718033209644837 25.27041934698205 30.640123784008367 25.371423659364744 4 12.191679740950088 17.48658542700968 38.96781218881414 31.35392264322609 5 13.126826752249412 38.07596423863831 34.84941830477391 13.94779070433836 6 29.977586984697567 37.879428002663786 18.232594109601795 13.910390903036857 7 27.068670480500046 33.78679287161606 21.416655330178212 17.727881317705684 8 25.453217630380614 35.55861041899362 20.343242546610536 18.644929404015237 9 25.415586832949128 17.8741713320256 21.089331481507998 35.620910353517274 10-11 24.125374609278495 27.756354483316688 27.677941645094183 20.440329262310637 12-13 25.18437898294405 24.824929470563806 28.43517736184107 21.555514184651077 14-15 21.988014348414442 26.606857740196325 26.746833539776755 24.658294371612477 16-17 21.075732298461297 29.729062099482324 27.55490000930812 21.64030559274826 18-19 21.067723435788388 28.11607277101188 30.19846639603067 20.617737397169062 20-21 21.670538792254796 27.175520052121556 30.435623078107156 20.718318077516493 22-23 21.378098197282323 27.001746874955256 30.464353727859795 21.155801199902633 24-25 21.074936288405922 27.484036308450015 30.403315866334506 21.037711536809553 26-27 21.112296325992954 27.560422095587185 30.212479596804215 21.114801981615646 28-29 20.93451988888571 27.94574586901114 30.0602823677654 21.059451874337753 30-31 21.178685989231298 27.499570397525492 30.101171382747165 21.22057223049605 32-33 20.731366793155296 27.770458938927472 30.051192095654038 21.446982172263194 34-35 21.721198494900058 27.690043928582934 29.806277455364334 20.78248012115267 36-37 21.548132132476123 27.31711507896734 29.788155615057487 21.34659717349905 38-39 21.001632279033807 28.05336406981572 29.748285391102648 21.196718260047824 40-41 21.480908762246347 27.7990757743343 29.74023603191478 20.979779431504568 42-43 21.101994644672594 27.850208342282745 29.468619786073287 21.579177226971378 44-45 21.12914542642763 27.887121255512916 29.663783721862654 21.319949596196803 46-47 21.606257495212013 28.086058458178954 29.003561903739104 21.30412214286993 48-49 21.261872115876862 28.360260765901383 29.011459522931577 21.366407595290177 50-51 21.509783846093264 28.514917411880948 28.69104812091445 21.28425062111134 52-53 21.543523481162442 28.438754211285712 28.691878886824266 21.32584342072758 54-55 21.345084716638684 28.405938219321765 28.795065564556836 21.453911499482714 56-57 21.47400447427293 28.43167785234899 28.676152125279643 21.418165548098433 58-59 21.355219970004903 28.410510453541605 28.57372959313334 21.66053998332015 60-61 21.157315274814952 28.34946382808138 29.077483642819306 21.415737254284366 62-63 21.299733323787876 28.437348987883237 29.093479856102235 21.169437832226656 64-65 21.24523506988564 28.615351575782523 28.71270826995007 21.426705084381766 66-67 21.11112304173691 28.52004166174528 28.604510492388858 21.76432480412895 68-69 21.47632750647826 28.25237297599107 28.750948474566567 21.52035104296411 70-71 21.440715883668904 28.45709172259508 28.315704697986575 21.78648769574944 72-73 21.552085160301957 28.179999212539236 28.550821643716954 21.71709398344185 74-75 21.22346708665927 28.645881805490923 28.530264523749864 21.60038658409994 76-77 21.26160697026754 28.398685557599922 28.504642788107017 21.835064684025514 78-79 21.366952313452604 28.38462805065756 28.461230070946357 21.78718956494348 80-81 21.344324848675754 28.55025826242899 28.27391925316877 21.831497635726482 82-83 21.32944852691147 28.856499593741837 28.309214364716027 21.504837514630662 84-85 21.419137779845737 28.621485710195245 28.319762343635357 21.639614166323664 86-87 21.315083986842716 28.715160582556937 28.156083453536105 21.813671977064235 88-89 21.394108593722034 28.62486130498586 28.174952575253233 21.80607752603887 90-91 21.265476620861715 28.707856521567937 28.201365199929846 21.8253016576405 92-93 21.43340360874374 28.643374365105217 28.31657580974812 21.606646216402925 94-95 21.28737146150019 28.480874473408235 28.307998468096677 21.923755596994894 96-97 21.32398469572189 28.34655814802487 28.26173322023343 22.067723936019814 98-99 21.330088052115396 28.560741642207745 27.944734769847518 22.164435535829334 100-101 21.46559005855488 28.35151541181692 28.071983850878325 22.110910678749875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 174.0 1 99.5 2 23.5 3 30.5 4 28.0 5 22.5 6 44.5 7 75.0 8 124.0 9 170.0 10 162.0 11 136.0 12 142.0 13 152.0 14 161.0 15 189.0 16 202.0 17 207.5 18 218.5 19 262.5 20 304.5 21 324.5 22 407.5 23 513.5 24 615.5 25 740.0 26 968.5 27 1262.5 28 1466.5 29 1714.0 30 2101.0 31 2447.0 32 2796.5 33 3093.5 34 3418.0 35 3823.5 36 4243.0 37 4577.0 38 4709.5 39 4786.0 40 4877.0 41 5021.0 42 5132.5 43 5183.0 44 5198.0 45 5273.5 46 5237.5 47 5178.0 48 5292.5 49 5136.0 50 4737.5 51 4463.5 52 4157.5 53 3953.0 54 3850.5 55 3541.5 56 3112.0 57 2686.0 58 2385.5 59 2078.5 60 1631.5 61 1314.5 62 1067.0 63 721.5 64 475.0 65 352.0 66 267.5 67 206.0 68 157.0 69 114.0 70 82.5 71 59.0 72 35.5 73 24.0 74 22.5 75 16.5 76 8.5 77 7.5 78 5.5 79 4.0 80 3.5 81 2.0 82 2.0 83 0.5 84 1.0 85 1.5 86 1.5 87 2.0 88 1.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2701292761535878 2 0.2472611892834428 3 0.23940028442183042 4 0.2472611892834428 5 0.24368805070998262 6 0.2015250155431528 7 0.1900909721080803 8 0.18937634439338827 9 0.17937155638769983 10-11 0.20545546797395897 12-13 0.19652262154030856 14-15 0.1900909721080803 16-17 0.1929494829668484 18-19 0.18723246124931217 20-21 0.18544589196258207 22-23 0.18223006724646795 24-25 0.17222527924077952 26-27 0.17794230095831576 28-29 0.18365932267585203 30-31 0.1922348552521564 32-33 0.1879470889640042 34-35 0.19616530768296253 36-37 0.18223006724646795 38-39 0.17937155638769983 40-41 0.17758498710096973 42-43 0.18508857810523607 44-45 0.18651783353462015 46-47 0.18616051967727412 48-49 0.19116291368011834 50-51 0.18866171667869625 52-53 0.1990238185417307 54-55 0.18687514739196617 56-57 0.17544110395689366 58-59 0.1740118485275096 60-61 0.17079602381139544 62-63 0.1790142425303538 64-65 0.17186796538343352 66-67 0.16900945452466537 68-69 0.16793751295262732 70-71 0.17544110395689366 72-73 0.1740118485275096 74-75 0.17722767324362373 76-77 0.18151543953177593 78-79 0.1786569286730078 80-81 0.17829961481566178 82-83 0.1740118485275096 84-85 0.16829482680997335 86-87 0.17043870995404944 88-89 0.17008139609670342 90-91 0.17615573167158569 92-93 0.1747264762422016 94-95 0.1697240822393574 96-97 0.1668655713805893 98-99 0.1736545346701636 100-101 0.16793751295262732 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 139933.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.85279390385612 #Duplication Level Percentage of deduplicated Percentage of total 1 91.63625787997753 75.92319988250391 2 5.594778384362448 9.270860408346623 3 1.2410731446999603 3.0847913243230933 4 0.5205578738170091 1.7251869693756083 5 0.2735747398794695 1.1333215770267369 6 0.18069736750217244 0.8982769049176106 7 0.1207952107793074 0.7005754492289571 8 0.07786771462989939 0.5161246169596271 9 0.052910082190308995 0.3945373321634785 >10 0.2785266259194818 3.9643966561597495 >50 0.015972853038169345 0.8858478633667606 >100 0.005989819889313505 1.0057805806293005 >500 9.98303314885584E-4 0.4971004349985277 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 601 0.42949125652991077 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 412 0.29442661845311685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 268 0.19152022753746437 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 176 0.1257744777857975 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.146277146920312E-4 2 0.0 0.0 0.0 0.0 7.146277146920312E-4 3 0.0 0.0 0.0 0.0 7.146277146920312E-4 4 0.0 0.0 0.0 0.0 7.146277146920312E-4 5 0.0 0.0 0.0 0.0 7.146277146920312E-4 6 0.0 0.0 0.0 0.0 7.146277146920312E-4 7 0.0 0.0 0.0 0.0 7.146277146920312E-4 8 0.0 0.0 0.0 0.0 7.146277146920312E-4 9 0.0 0.0 0.0 0.0 7.146277146920312E-4 10-11 0.0 0.0 0.0 0.0 7.146277146920312E-4 12-13 0.0 0.0 0.0 0.0 7.146277146920312E-4 14-15 0.0 0.0 0.0 0.0 7.146277146920312E-4 16-17 0.0 0.0 0.0 0.0 7.146277146920312E-4 18-19 0.0 0.0 0.0 0.0 7.146277146920312E-4 20-21 0.0 0.0 0.0 0.0 7.146277146920312E-4 22-23 0.0 0.0 0.0 0.0 7.146277146920312E-4 24-25 0.0 0.0 0.0 0.0 7.146277146920312E-4 26-27 0.0 0.0 0.0 0.0 7.146277146920312E-4 28-29 0.0 0.0 0.0 0.0028585108587681246 7.146277146920312E-4 30-31 0.0 0.0 0.0 0.007503591004266327 7.146277146920312E-4 32-33 0.0 0.0 0.0 0.01393524043649461 7.146277146920312E-4 34-35 0.0 0.0 0.0 0.016436437437916715 7.146277146920312E-4 36-37 0.0 0.0 0.0 0.02536928387156711 7.146277146920312E-4 38-39 0.0 0.0 0.0 0.03287287487583343 7.146277146920312E-4 40-41 0.0 0.0 0.0 0.040376465880099764 7.146277146920312E-4 42-43 3.573138573460156E-4 0.0 0.0 0.05181050931517226 7.146277146920312E-4 44-45 7.146277146920312E-4 0.0 0.0 0.07289202689858718 7.146277146920312E-4 46-47 0.0014292554293840623 0.0 0.0 0.10183444934361444 7.146277146920312E-4 48-49 0.0014292554293840623 0.0 0.0 0.16686557138058927 7.146277146920312E-4 50-51 0.0014292554293840623 0.0 0.0 0.23011012413083404 7.146277146920312E-4 52-53 0.0014292554293840623 0.0 0.0 0.28799496902088856 7.146277146920312E-4 54-55 0.0014292554293840623 0.0 0.0 0.40019152022753746 7.146277146920312E-4 56-57 0.0014292554293840623 0.0 0.0 0.4995247725697298 7.146277146920312E-4 58-59 0.0014292554293840623 0.0 0.0 0.5863520399048117 7.146277146920312E-4 60-61 0.0014292554293840623 0.0 0.0 0.6828267813882358 7.146277146920312E-4 62-63 0.0014292554293840623 0.0 0.0 0.8300400906147942 7.146277146920312E-4 64-65 0.0014292554293840623 0.0 0.0 0.9283014013849484 7.146277146920312E-4 66-67 0.0014292554293840623 0.0 0.0 1.0672964918925487 7.146277146920312E-4 68-69 0.0014292554293840623 0.0 0.0 1.280970178585466 7.146277146920312E-4 70-71 0.0014292554293840623 0.0 0.0 1.4403321589617888 7.146277146920312E-4 72-73 0.0014292554293840623 0.0 0.0 1.712605318259453 7.146277146920312E-4 74-75 0.0014292554293840623 0.0 0.0 2.0556266213116277 7.146277146920312E-4 76-77 0.0014292554293840623 0.0 0.0 2.29574153344815 7.146277146920312E-4 78-79 0.0014292554293840623 0.0 0.0 2.540144211872825 7.146277146920312E-4 80-81 0.0014292554293840623 0.0 0.0 2.903889718651069 0.0010719415720380468 82-83 0.0014292554293840623 0.0 0.0 3.2890740568700734 0.0014292554293840623 84-85 0.0014292554293840623 0.0 0.0 3.713920233254486 0.0014292554293840623 86-87 0.0014292554293840623 0.0 0.0 4.215946202825638 0.0014292554293840623 88-89 0.0014292554293840623 0.0 0.0 4.788363002293955 0.0014292554293840623 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCTTGT 50 2.8129167E-5 47.50358 1 GTCTTAT 40 5.3698634E-4 47.503574 1 GGTATCA 390 0.0 46.285538 1 GTATCAA 590 0.0 34.62125 1 TAAGGTG 55 0.0025664552 34.560417 5 GTATAGG 55 0.0025709644 34.548058 1 GTGTAAG 60 0.003937742 31.66905 1 ACTGTAC 95 3.4674013E-5 30.00226 8 TATAGGA 100 4.919946E-5 28.502146 2 TAGGGCA 85 6.362706E-4 27.95328 4 TCTAAGG 85 6.376041E-4 27.943281 3 TCAACGC 715 0.0 27.914183 4 ATCAACG 720 0.0 27.71042 3 ACATGGC 105 6.859738E-5 27.144901 3 CAATATC 35 0.008377828 27.1449 62-63 CAACGCA 745 0.0 26.790121 5 AACGCAG 745 0.0 26.780539 6 TATCAAC 765 0.0 26.080395 2 TGCACTG 110 9.390671E-5 25.920313 5 TAATACA 100 0.0016422675 23.760286 4 >>END_MODULE