FastQCFastQC Report
Thu 26 May 2016
SRR937226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139933
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8110.5795630766152373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.47379817484081665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.3108630558910336No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.12934761635925765No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG1430.10219176320096046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7550.040.9085041
GGTATCA4350.039.324251
TAAGGCA851.6220616E-533.5292854
ATCAACG9300.032.17733
TATCAAC9500.031.9998782
TCAACGC9450.031.666554
ATGGGAT1509.404175E-1031.6665485
CAACGCA9500.031.499885
AACGCAG9800.030.5355996
CATGGGA2600.029.230664
CGCAGAG10850.027.5805428
ACGCAGA11100.026.9593587
TACATGG4600.025.8151192
TAGCCCT1501.0490457E-625.3332394
GCAGAGT11700.025.1708459
TGGGATG1351.3318346E-524.6295366
TCCTAAG1751.466833E-724.4284782
ACATGGG4700.024.2552283
GTACATG5350.023.9804441
AGAGTAC10100.023.74991210-11
Produced by FastQC (version 0.11.5)