##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937226_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 139933 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20185374429191 33.0 31.0 34.0 31.0 34.0 2 32.427233032951484 34.0 31.0 34.0 31.0 34.0 3 32.49978203854702 34.0 31.0 34.0 31.0 34.0 4 36.119985993296794 37.0 35.0 37.0 35.0 37.0 5 35.97626721359508 37.0 35.0 37.0 35.0 37.0 6 36.058277890133134 37.0 35.0 37.0 35.0 37.0 7 36.034302130305214 37.0 35.0 37.0 35.0 37.0 8 36.00641021060079 37.0 35.0 37.0 35.0 37.0 9 37.73040669463243 39.0 37.0 39.0 35.0 39.0 10-11 37.63745149464387 39.0 37.0 39.0 35.0 39.0 12-13 37.56234769496831 39.0 37.0 39.0 35.0 39.0 14-15 38.78377509236563 40.0 38.0 41.0 35.0 41.0 16-17 38.755022046265 40.0 38.0 41.0 35.0 41.0 18-19 38.7735201846598 40.0 38.0 41.0 35.0 41.0 20-21 38.697769646902444 40.0 38.0 41.0 34.0 41.0 22-23 38.58294683884431 40.0 38.0 41.0 34.0 41.0 24-25 38.43213537907427 40.0 38.0 41.0 34.0 41.0 26-27 38.21015771833663 40.0 38.0 41.0 34.0 41.0 28-29 38.192434951012274 40.0 38.0 41.0 34.0 41.0 30-31 38.29879656692846 40.0 38.0 41.0 34.0 41.0 32-33 38.23539836921955 40.0 38.0 41.0 34.0 41.0 34-35 38.114304702964986 40.0 38.0 41.0 34.0 41.0 36-37 37.943015586030455 40.0 37.0 41.0 33.0 41.0 38-39 37.79591304409968 40.0 37.0 41.0 33.0 41.0 40-41 37.63547912215132 40.0 37.0 41.0 33.0 41.0 42-43 37.425067710975966 40.0 37.0 41.0 33.0 41.0 44-45 37.130944809301596 40.0 37.0 41.0 31.5 41.0 46-47 36.80437066310306 40.0 36.0 41.0 31.0 41.0 48-49 36.45796917096039 39.5 36.0 41.0 30.0 41.0 50-51 36.2326184674094 39.0 36.0 41.0 30.0 41.0 52-53 36.00584208156761 39.0 35.0 41.0 29.5 41.0 54-55 35.82032472683356 39.0 35.0 40.0 29.0 41.0 56-57 35.578076650968676 39.0 35.0 40.0 28.0 41.0 58-59 35.34602988573103 38.5 35.0 40.0 28.0 41.0 60-61 35.027595349202834 38.0 34.5 40.0 27.0 41.0 62-63 34.691077158354354 38.0 34.0 40.0 26.5 41.0 64-65 34.340738067503736 37.0 34.0 40.0 26.0 41.0 66-67 33.965955135672075 37.0 34.0 39.5 26.0 41.0 68-69 33.5149035609899 36.0 33.0 39.0 25.0 40.5 70-71 33.15272308890684 36.0 33.0 39.0 24.0 40.0 72-73 32.72331758770268 35.0 32.0 38.0 22.5 40.0 74-75 32.287634082024965 35.0 32.0 37.5 22.0 39.5 76-77 30.969417506949753 34.0 30.5 36.0 20.5 38.5 78-79 31.603860418914763 35.0 31.5 36.0 21.0 39.0 80-81 31.39927322361416 35.0 31.5 36.0 20.0 38.5 82-83 31.04998463550413 34.5 31.0 36.0 19.5 37.0 84-85 30.697487368955144 34.0 31.0 35.0 18.0 37.0 86-87 30.361733829761384 34.0 31.0 35.0 16.0 36.5 88-89 29.99341113247054 34.0 30.5 35.0 9.0 36.0 90-91 29.54184502583379 34.0 30.0 35.0 4.5 36.0 92-93 29.113700842546073 34.0 29.5 35.0 2.0 35.0 94-95 28.697158640206382 34.0 29.0 35.0 2.0 35.0 96-97 28.292547147563475 34.0 29.0 35.0 2.0 35.0 98-99 27.66041248311692 33.5 28.0 35.0 2.0 35.0 100-101 26.016243487954952 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0415013147096559 1101 2 0.053997454856464344 1101 3 0.020130010657332775 1101 4 0.008245940837795729 1101 5 -0.02791821256644056 1101 6 -0.07444489104961605 1101 7 0.18504719978740525 1101 8 0.1281356430758649 1101 9 0.15606910106101424 1101 10-11 -0.0028530279860419228 1101 12-13 0.16697916259281698 1101 14-15 0.20881916092848485 1101 16-17 0.2590322704988708 1101 18-19 0.13340376506099716 1101 20-21 -0.1233327638965136 1101 22-23 0.22172420209393096 1101 24-25 0.18438761743392007 1101 26-27 -0.15369664583023734 1101 28-29 -0.130684254808358 1101 30-31 0.18829763022734625 1101 32-33 0.39966871790854697 1101 34-35 0.15058903133737545 1101 36-37 0.44379346815644993 1101 38-39 0.28952966432184013 1101 40-41 0.3549150470305733 1101 42-43 0.021252245460459562 1101 44-45 0.3379923512674594 1101 46-47 0.6518593464547067 1101 48-49 0.9298365937758675 1101 50-51 0.8473965484695398 1101 52-53 0.9682515065625878 1101 54-55 1.0694660196936425 1101 56-57 1.0902502991301901 1101 58-59 1.051786258217689 1101 60-61 1.0142573648803008 1101 62-63 0.7953092030912217 1101 64-65 0.6550891128360661 1101 66-67 0.8047409452127638 1101 68-69 0.3168437997771676 1101 70-71 0.2607246806022445 1101 72-73 0.47610497181417344 1101 74-75 0.30845381843206354 1101 76-77 0.2568836628406288 1101 78-79 0.4326108844073637 1101 80-81 0.2656820188025293 1101 82-83 0.7800142450667273 1101 84-85 0.3835375108914967 1101 86-87 0.8280150733628737 1101 88-89 0.7562142149402291 1101 90-91 0.8652980646103643 1101 92-93 0.5257776954414624 1101 94-95 0.8417726265222392 1101 96-97 1.2334381947095778 1101 98-99 0.755770125118147 1101 100-101 1.5295982368219008 1102 1 0.2711180394483321 1102 2 0.31042167088434525 1102 3 0.24613175281761102 1102 4 0.029173641186226007 1102 5 0.11378474910603131 1102 6 0.10107775703401245 1102 7 0.19713343672120232 1102 8 0.2958185699051299 1102 9 0.20541579095648643 1102 10-11 0.4194792890871284 1102 12-13 0.059596758411636586 1102 14-15 0.10348379507482264 1102 16-17 0.1734595353072308 1102 18-19 0.10214287655925602 1102 20-21 0.233265668159234 1102 22-23 0.1751976341496828 1102 24-25 0.46463587527364325 1102 26-27 0.3427537026018186 1102 28-29 0.3833728009407622 1102 30-31 0.34654022604615875 1102 32-33 0.4778917144242243 1102 34-35 0.673345999978487 1102 36-37 0.839851830525788 1102 38-39 0.7291746991650427 1102 40-41 0.8764851689817874 1102 42-43 1.062367228038859 1102 44-45 0.7962240585845279 1102 46-47 0.8986799039459967 1102 48-49 0.9556096773995577 1102 50-51 1.3742584822674502 1102 52-53 1.2182950605695666 1102 54-55 1.1563344865926055 1102 56-57 1.616208487283501 1102 58-59 1.628114655430231 1102 60-61 1.5241659014656648 1102 62-63 1.4031053703386078 1102 64-65 1.4809928584806684 1102 66-67 1.5073106316239162 1102 68-69 1.3510010297423207 1102 70-71 1.5065870499402294 1102 72-73 1.6244716965528454 1102 74-75 1.2810365710452984 1102 76-77 1.0390700739904588 1102 78-79 1.206097382665206 1102 80-81 1.2983366355272636 1102 82-83 1.3127668583071426 1102 84-85 0.9293781032259929 1102 86-87 1.489611327718272 1102 88-89 1.2271636922921445 1102 90-91 1.3013281168751725 1102 92-93 1.6205294376017392 1102 94-95 1.5576371735605647 1102 96-97 1.254180790528391 1102 98-99 1.2919199509021162 1102 100-101 1.0383961462295659 1103 1 -0.10135582814749 1103 2 -0.16730356555169834 1103 3 -0.20563529546512171 1103 4 0.09689390002147036 1103 5 -0.2058518860358305 1103 6 -0.10250611553941269 1103 7 0.032623730399649276 1103 8 0.02354380634117348 1103 9 0.017038488816119468 1103 10-11 -0.14794231370032662 1103 12-13 -0.009679000672491611 1103 14-15 -0.15789002274497932 1103 16-17 -0.2081233417460311 1103 18-19 0.23895223444875313 1103 20-21 0.04057029732797446 1103 22-23 -0.41443651219178435 1103 24-25 -0.07645421930077134 1103 26-27 0.0038288643738439987 1103 28-29 -0.0436306833797957 1103 30-31 -0.09773553303796234 1103 32-33 0.19303606484731972 1103 34-35 -0.23270178498915328 1103 36-37 0.033716937544205905 1103 38-39 0.022629154117758787 1103 40-41 -0.002546687663304681 1103 42-43 -0.7466048973966792 1103 44-45 -0.41073213852845925 1103 46-47 -0.6733957555861139 1103 48-49 -0.5711200388771971 1103 50-51 -0.5196315127549482 1103 52-53 -0.4820035954782256 1103 54-55 -0.6046411231634963 1103 56-57 -0.5500558233187931 1103 58-59 -0.563647415251701 1103 60-61 -0.3779620228748044 1103 62-63 -0.6345377356842903 1103 64-65 -0.7323471116537235 1103 66-67 -0.8113304833586596 1103 68-69 -0.5291383430799783 1103 70-71 -0.6078977683773417 1103 72-73 -0.6520838036960299 1103 74-75 -0.5544288346291637 1103 76-77 -0.7545959289961033 1103 78-79 -0.7921977890620227 1103 80-81 -1.319458287319918 1103 82-83 -1.1842714692189844 1103 84-85 -0.6630186115574794 1103 86-87 -0.6939874776575365 1103 88-89 -1.1659796626107877 1103 90-91 -0.7676738741651441 1103 92-93 -0.4863396514973175 1103 94-95 -0.5934952306206185 1103 96-97 -0.015605172637364717 1103 98-99 -0.3998421197798159 1103 100-101 -0.30968747746381453 1104 1 0.09705687026521304 1104 2 0.15336914797816092 1104 3 -0.03707898405166077 1104 4 0.07289541173726377 1104 5 0.011268295370065573 1104 6 0.016163574558852645 1104 7 0.06021254370274676 1104 8 -0.023319383379160286 1104 9 -0.009605502113586795 1104 10-11 -0.0037624195204344346 1104 12-13 0.18731646418012105 1104 14-15 -6.715798122556293E-4 1104 16-17 0.05491520700680752 1104 18-19 -0.02540906562683176 1104 20-21 0.062017765204011255 1104 22-23 0.2039497311944558 1104 24-25 -0.08760312330682041 1104 26-27 0.36134965046606027 1104 28-29 0.11377672402761618 1104 30-31 -0.15272419516947622 1104 32-33 -0.2577386894988649 1104 34-35 0.32039723239557816 1104 36-37 0.5067894999024816 1104 38-39 0.19288614580332109 1104 40-41 0.43254401528454167 1104 42-43 0.4101411343493524 1104 44-45 0.31649764227275057 1104 46-47 0.13234876444412436 1104 48-49 0.6117975726118416 1104 50-51 0.9284977389457296 1104 52-53 1.0036351044461966 1104 54-55 0.7630837445613707 1104 56-57 0.8984513573312469 1104 58-59 0.984160596842031 1104 60-61 0.49854969292263007 1104 62-63 1.0191848644668795 1104 64-65 0.883677075799028 1104 66-67 0.5947541727259917 1104 68-69 0.5660997521581166 1104 70-71 0.7061611885387578 1104 72-73 0.641944918904116 1104 74-75 0.753394294622538 1104 76-77 1.2783783718353376 1104 78-79 1.4361657785872595 1104 80-81 1.398039823035333 1104 82-83 0.8382153032677877 1104 84-85 0.8383986220026074 1104 86-87 0.8953927188655157 1104 88-89 0.8894814107074254 1104 90-91 0.9363132762505764 1104 92-93 1.2309694943832596 1104 94-95 1.5050497429243528 1104 96-97 0.7617946761910588 1104 98-99 1.092410336758359 1104 100-101 1.4045982368219008 1105 1 0.0575186307269675 1105 2 0.03340762801663999 1105 3 0.12299797602529594 1105 4 0.08164962049147562 1105 5 -0.2282699441681757 1105 6 0.027611386006661576 1105 7 -0.25253396904376757 1105 8 -0.26353102359080083 1105 9 -0.01941791192599851 1105 10-11 -0.054652270410286974 1105 12-13 -0.042842265978613625 1105 14-15 0.009766130625450842 1105 16-17 0.1945977466893467 1105 18-19 0.009607769390008514 1105 20-21 -0.25106544787919916 1105 22-23 0.25753318477791254 1105 24-25 0.2059514702477756 1105 26-27 0.12479361391002186 1105 28-29 0.3098325200834111 1105 30-31 0.08046483801955162 1105 32-33 -0.12565595741613578 1105 34-35 -0.11009819809985544 1105 36-37 -0.1608872677742852 1105 38-39 0.0555605084776829 1105 40-41 -0.14783116509063632 1105 42-43 -0.28567416146594127 1105 44-45 -0.08965916388405759 1105 46-47 -0.27005623796087974 1105 48-49 0.07408713490141139 1105 50-51 0.20382241427040526 1105 52-53 0.31873851954960486 1105 54-55 0.4135406950183196 1105 56-57 0.2168736757535683 1105 58-59 0.2840643967458263 1105 60-61 0.09474978912272292 1105 62-63 0.10672695200896953 1105 64-65 0.13413402625597826 1105 66-67 0.015292893264721386 1105 68-69 -0.2269738409154769 1105 70-71 0.3917311741087417 1105 72-73 0.2169328938920927 1105 74-75 0.30187914310738506 1105 76-77 0.12436201781898859 1105 78-79 0.4831114255329041 1105 80-81 0.3080927330882446 1105 82-83 -0.0022368371843555224 1105 84-85 0.05066413426812133 1105 86-87 0.35465197812477456 1105 88-89 0.7253159465419614 1105 90-91 0.1333310732683728 1105 92-93 0.22163808505185045 1105 94-95 0.6297731676477802 1105 96-97 0.4032569176532981 1105 98-99 1.0024151467631661 1105 100-101 0.9582778905015559 1106 1 -0.0050424640921917785 1106 2 0.00955621061683587 1106 3 -0.01932851468829 1106 4 0.003839258810145907 1106 5 0.19088939112019432 1106 6 -0.15635733344592495 1106 7 -0.24960141772411504 1106 8 0.06765177210812112 1106 9 -0.5050484104597217 1106 10-11 -0.2186796408892704 1106 12-13 -0.4460680724302293 1106 14-15 -0.31476954874893437 1106 16-17 -0.20325171567625233 1106 18-19 -0.2919562579804733 1106 20-21 -0.4329813814080339 1106 22-23 -0.5652429638046925 1106 24-25 -0.2505490185107746 1106 26-27 0.10837132651999326 1106 28-29 -0.20883805665167898 1106 30-31 -0.20751170156989218 1106 32-33 -0.16231284891173203 1106 34-35 0.021182349309718518 1106 36-37 -0.049841324470271786 1106 38-39 -0.3300699900560389 1106 40-41 0.044935208320893594 1106 42-43 0.27102183071391295 1106 44-45 0.0609762222351975 1106 46-47 0.4539906828602298 1106 48-49 -0.02464209286008412 1106 50-51 0.2942427466262245 1106 52-53 0.010820630986557944 1106 54-55 0.2631008123203742 1106 56-57 0.10660974613480079 1106 58-59 -0.046629640399821426 1106 60-61 -0.06820231253905007 1106 62-63 0.24260182981932843 1106 64-65 -0.06684349085056596 1106 66-67 0.5276096088072393 1106 68-69 0.7722441453992843 1106 70-71 0.30052882806769077 1106 72-73 0.39024667688329373 1106 74-75 0.5523190258053248 1106 76-77 0.5670795153751946 1106 78-79 0.7553499397069032 1106 80-81 0.7306733782495343 1106 82-83 0.40489903769345403 1106 84-85 0.448024838080439 1106 86-87 0.7503509028559598 1106 88-89 1.1384146757697238 1106 90-91 1.194719147559674 1106 92-93 1.048617557192614 1106 94-95 0.9439471656927445 1106 96-97 1.0936772500091152 1106 98-99 1.7526595260398032 1106 100-101 1.0640452414301969 1107 1 0.013113036321378502 1107 2 -0.12125437664536065 1107 3 -0.09273628970896652 1107 4 -0.24049490165305087 1107 5 -0.22652169241992226 1107 6 -0.16189910350382775 1107 7 -0.26721928372907655 1107 8 0.03518692512714949 1107 9 -0.32676056926865726 1107 10-11 -0.08338187413988862 1107 12-13 -0.28963713777348943 1107 14-15 0.11186403272336065 1107 16-17 -0.20988943279783712 1107 18-19 0.08000403978627446 1107 20-21 -0.2358556576694113 1107 22-23 -0.07469292244819314 1107 24-25 0.05927314856945287 1107 26-27 0.04536470948112026 1107 28-29 -0.2693632841123943 1107 30-31 -0.37128341372869755 1107 32-33 -0.34849978025995654 1107 34-35 -0.42186975987142006 1107 36-37 0.07943907255205573 1107 38-39 0.18667938959656283 1107 40-41 -0.1286586709181421 1107 42-43 -0.030487681279467438 1107 44-45 0.4838256846007951 1107 46-47 0.12003700213235646 1107 48-49 0.26208247289674347 1107 50-51 0.4747431601911529 1107 52-53 0.25877348458457305 1107 54-55 -0.450794969317343 1107 56-57 -0.21698413310424058 1107 58-59 0.0361040237854553 1107 60-61 0.25704582641876783 1107 62-63 0.03912788440990056 1107 64-65 -0.2500734329514813 1107 66-67 -0.21471876174694415 1107 68-69 -0.5488269877686278 1107 70-71 -0.6497606673830987 1107 72-73 -0.40084099888179736 1107 74-75 -0.23378519255695096 1107 76-77 -0.1916053481483786 1107 78-79 0.19033753275901333 1107 80-81 -0.14383034383483206 1107 82-83 -0.3468172567647727 1107 84-85 0.07618860979259878 1107 86-87 0.29567761915041757 1107 88-89 0.006901028127046516 1107 90-91 0.15553387047117084 1107 92-93 0.43935370276746966 1107 94-95 0.17802491589952396 1107 96-97 0.604946894343275 1107 98-99 0.15503752438554486 1107 100-101 0.35285831008197377 1108 1 -0.24761433154884926 1108 2 0.0452814684619085 1108 3 -0.042369989342667225 1108 4 0.07818641702827023 1108 5 0.10197124321587125 1108 6 -0.32699591145777873 1108 7 0.015616927678557602 1108 8 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0.3255461245702165 2316 62-63 -0.2357677625787744 2316 64-65 0.12835530884935764 2316 66-67 0.20112798840214197 2316 68-69 0.29755047198093365 2316 70-71 0.36544505712495123 2316 72-73 0.2596390249703191 2316 74-75 0.3425415814442445 2316 76-77 0.26262840259700226 2316 78-79 0.4777555411072534 2316 80-81 0.4825113377394068 2316 82-83 0.22242842003904428 2316 84-85 0.44932516316170634 2316 86-87 0.5616287223108216 2316 88-89 0.38585153498452485 2316 90-91 -0.015083303052978891 2316 92-93 0.07709967772274595 2316 94-95 -0.5593036470104344 2316 96-97 -0.9234520323114666 2316 98-99 -0.3407138172960309 2316 100-101 -0.41599976982261566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 18.0 8 25.0 9 68.0 10 132.0 11 209.0 12 289.0 13 396.0 14 550.0 15 761.0 16 1038.0 17 1370.0 18 1343.0 19 785.0 20 645.0 21 665.0 22 672.0 23 742.0 24 848.0 25 974.0 26 1179.0 27 1375.0 28 1736.0 29 2203.0 30 2819.0 31 3620.0 32 4546.0 33 6135.0 34 8359.0 35 12425.0 36 19517.0 37 32069.0 38 29395.0 39 3021.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.13370222622571 20.543616580162713 16.530834012496605 21.79184718111497 2 15.152251434615138 20.875704801583613 40.83239836207328 23.13964540172797 3 18.661788141467703 25.272809130083683 31.42861226444084 24.636790464007774 4 12.57816240629444 18.265884387528317 38.59489898737253 30.561054218804713 5 12.975800803304699 37.91683938194137 35.080973685338975 14.026386129414961 6 30.24375951348145 38.2297242251649 17.456211186782244 14.070305074571401 7 26.784961374372017 34.078451830518894 21.393095267020644 17.74349152808844 8 25.820928587252475 35.46340034159205 19.90952813131999 18.806142939835492 9 25.557230960531115 17.24682526637748 21.012198695089793 36.183745078001614 10-11 24.204083382761752 27.422409295877316 27.859046829554146 20.514460491806794 12-13 25.397976794960208 24.700825076022053 28.2998574251482 21.601340703869532 14-15 22.130233754725477 26.154659730013652 27.171574968020412 24.543531547240466 16-17 21.070083539979848 29.334753060393187 28.019838065359853 21.575325334267113 18-19 20.925371427754712 27.09904025497917 31.282828210643665 20.692760106622455 20-21 21.37201374943723 26.42193049530847 31.501861605196773 20.70419415005753 22-23 21.263922189388225 26.273945093779368 31.480852858046372 20.98127985878603 24-25 21.159487610097727 26.39915673628356 31.637396601933077 20.803959051685634 26-27 20.916247110151897 26.842445660136 31.29611697319007 20.945190256522032 28-29 20.735515868762015 27.0329953047617 31.41200180091332 20.819487025562964 30-31 20.875645854028058 26.719240196954214 31.19573218228984 21.209381766727887 32-33 20.7334938863599 26.787462571373442 31.043070612364488 21.435972929902167 34-35 21.242308819220625 26.78424674665733 31.266391773205747 20.707052660916293 36-37 21.2306691821742 26.46678386644942 31.259826201297773 21.042720750078608 38-39 20.974755685777073 26.831508048523396 31.047826630696946 21.145909635002592 40-41 21.232022582316475 26.88010290675862 30.980294070355352 20.90758044056956 42-43 21.041892244413713 26.79452681382376 30.839024146199396 21.324556795563133 44-45 21.27518169409646 26.88536656828625 30.73149292875876 21.107958808858527 46-47 21.512080781516868 27.31057005852801 30.042591811795642 21.13475734815948 48-49 21.426402015257356 27.489325210369287 29.77185428688832 21.312418487485036 50-51 21.488855380789378 27.52317180364889 29.871081160269558 21.116891655292175 52-53 21.899844567916674 27.450735175888376 29.503153306058277 21.14626695013667 54-55 21.17120336160877 27.687536178028054 29.796045250226893 21.345215210136278 56-57 21.486711497645302 27.579270079252215 29.607383533548198 21.326634889554285 58-59 21.29233275924907 27.469932038904332 29.894663874854395 21.343071326992202 60-61 21.233375972786977 27.751495358492996 29.94004273473734 21.07508593398269 62-63 21.06436651826231 27.75649775249584 30.097975459684278 21.081160269557575 64-65 21.056862927258045 27.77150493450437 29.7503090764866 21.42132306175098 66-67 21.141188997591705 27.91871824373093 29.468031129183252 21.472061629494117 68-69 21.4509801119107 27.60428204926643 29.59201903768232 21.352718801140547 70-71 21.30305217496945 27.902639120150358 29.406930459577087 21.38737824530311 72-73 21.360222392144813 27.355948918410956 29.494115040769508 21.78971364867472 74-75 21.331637283557132 27.683248411739903 29.26900731064152 21.716106994061445 76-77 21.101884473283643 27.643586573574495 29.456239771890836 21.798289181251025 78-79 21.33342385284386 27.84832741383376 29.30438138251878 21.513867350803597 80-81 21.326277575696942 27.861548026555568 29.16967405829933 21.642500339448166 82-83 21.488855380789378 27.95373500175084 29.163957036581795 21.39345258087799 84-85 21.282685285100726 27.986250562769328 29.216839487469002 21.514224664660944 86-87 21.131184209586014 28.016622240643734 28.8673865349846 21.98480701478565 88-89 21.012913322804486 27.926936462449888 29.059978704094103 22.000171510651526 90-91 21.07736287296766 27.774522065392176 29.257816687511166 21.890298374128996 92-93 21.03393815523365 27.327754393236663 29.47488404999607 22.163423401533613 94-95 20.80066889154095 27.52209986207685 29.498402807057662 22.178828439324533 96-97 20.928587252470827 27.292704365660708 29.42622540787377 22.352482973994697 98-99 20.6570404593709 27.3921696842361 29.154830433679578 22.79595942271342 100-101 20.53288735464597 27.451989393711983 29.138346305309575 22.87677694633247 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 22.0 1 18.5 2 16.0 3 25.5 4 24.0 5 16.0 6 48.0 7 119.5 8 208.5 9 256.0 10 217.5 11 158.5 12 152.5 13 191.0 14 196.5 15 196.0 16 224.5 17 231.5 18 249.0 19 277.5 20 309.0 21 354.5 22 430.0 23 524.5 24 628.5 25 780.0 26 954.5 27 1174.5 28 1445.0 29 1739.5 30 2048.5 31 2368.5 32 2833.0 33 3216.0 34 3459.5 35 3759.5 36 4136.5 37 4475.5 38 4698.5 39 4811.5 40 4945.5 41 5046.5 42 5023.5 43 5039.5 44 5099.0 45 5228.0 46 5316.5 47 5338.5 48 5402.5 49 5195.5 50 4792.0 51 4466.0 52 4165.0 53 3973.5 54 3839.0 55 3514.5 56 3062.0 57 2643.5 58 2372.5 59 2053.5 60 1589.0 61 1281.0 62 1065.0 63 767.0 64 474.0 65 334.0 66 239.0 67 182.5 68 139.0 69 102.0 70 78.0 71 52.5 72 34.0 73 28.0 74 21.0 75 17.5 76 13.5 77 5.5 78 4.0 79 2.0 80 0.5 81 1.0 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039304524308061715 2 0.0 3 0.0 4 0.0 5 0.007860904861612344 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004645080145498203 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.002501197001422109 24-25 3.573138573460156E-4 26-27 0.002501197001422109 28-29 0.0028585108587681246 30-31 0.0014292554293840623 32-33 0.0 34-35 0.0 36-37 7.146277146920312E-4 38-39 3.573138573460156E-4 40-41 3.573138573460156E-4 42-43 0.010362101863034451 44-45 0.0 46-47 0.0 48-49 3.573138573460156E-4 50-51 0.0 52-53 3.573138573460156E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.005717021717536249 92-93 0.0014292554293840623 94-95 0.0 96-97 0.0 98-99 0.001786569286730078 100-101 0.011434043435072498 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 139933.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.88978521541469 #Duplication Level Percentage of deduplicated Percentage of total 1 90.97076933500409 73.58605992389514 2 6.007479888363939 9.718875157113649 3 1.381752530622426 3.3530899626871133 4 0.5584983134105144 1.8070723445979147 5 0.2886153537648198 1.16730169879536 6 0.17175987448946972 0.8336171613647874 7 0.1178341078418634 0.6672102972064841 8 0.08787615437174669 0.5686626602149772 9 0.051926806997098285 0.3780313438425816 >10 0.3375242326519908 4.800513226436687 >50 0.016976070853632525 0.9321541746031787 >100 0.006990146822083981 0.9967749146103316 >500 0.001997184806309709 1.19063713463179 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 811 0.5795630766152373 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 663 0.47379817484081665 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 435 0.3108630558910336 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 181 0.12934761635925765 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 143 0.10219176320096046 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0035731385734601557 0.0 30-31 0.0 0.0 0.0 0.00821821871895836 0.0 32-33 0.0 0.0 0.0 0.01464986815118664 0.0 34-35 0.0 0.0 0.0 0.01715106515260875 7.146277146920312E-4 36-37 0.0 0.0 0.0 0.02608391158625914 7.146277146920312E-4 38-39 0.0 0.0 0.0 0.03501675801990953 7.146277146920312E-4 40-41 0.0 0.0 0.0 0.043949604453559915 7.146277146920312E-4 42-43 0.0 0.0 0.0 0.05574096174597843 7.146277146920312E-4 44-45 0.0 0.0 0.0 0.07575053775735531 0.0010719415720380468 46-47 0.0 0.0 0.0 0.10397833248769053 0.0014292554293840623 48-49 0.0 0.0 0.0 0.16900945452466537 0.0014292554293840623 50-51 0.0 0.0 0.0 0.2315393795602181 0.0014292554293840623 52-53 0.0 0.0 0.0 0.29121079373700276 0.0014292554293840623 54-55 0.0 0.0 0.0 0.40447928651568965 0.0014292554293840623 56-57 0.0 0.0 0.0 0.5055991081446121 0.0014292554293840623 58-59 0.0 0.0 0.0 0.5920690616223478 0.0014292554293840623 60-61 0.0 0.0 0.0 0.68782917539108 0.0014292554293840623 62-63 0.0 0.0 0.0 0.8346851707602925 0.0014292554293840623 64-65 0.0 0.0 0.0 0.9318745399584087 0.0014292554293840623 66-67 0.0 0.0 0.0 1.0712269443233549 0.0014292554293840623 68-69 0.0 0.0 0.0 1.288116455732386 0.0014292554293840623 70-71 0.0 0.0 0.0 1.4474784361087092 0.0014292554293840623 72-73 0.0 0.0 0.0 1.7229674201224872 0.0014292554293840623 74-75 0.0 0.0 0.0 2.0706338033201606 0.0014292554293840623 76-77 0.0 0.0 0.0 2.312177970886067 0.0014292554293840623 78-79 0.0 0.0 0.0 2.5580099047401257 0.0014292554293840623 80-81 0.0 0.0 0.0 2.9249712362344837 0.0014292554293840623 82-83 0.0 0.0 0.0 3.319088420887139 0.0014292554293840623 84-85 0.0 0.0 0.0 3.7496516189890876 0.0014292554293840623 86-87 0.0 0.0 0.0 4.259895807279198 0.0014292554293840623 88-89 0.0 0.0 0.0 4.842317394753204 0.0014292554293840623 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 755 0.0 40.908504 1 GGTATCA 435 0.0 39.32425 1 TAAGGCA 85 1.6220616E-5 33.529285 4 ATCAACG 930 0.0 32.1773 3 TATCAAC 950 0.0 31.999878 2 TCAACGC 945 0.0 31.66655 4 ATGGGAT 150 9.404175E-10 31.666548 5 CAACGCA 950 0.0 31.49988 5 AACGCAG 980 0.0 30.535599 6 CATGGGA 260 0.0 29.23066 4 CGCAGAG 1085 0.0 27.580542 8 ACGCAGA 1110 0.0 26.959358 7 TACATGG 460 0.0 25.815119 2 TAGCCCT 150 1.0490457E-6 25.333239 4 GCAGAGT 1170 0.0 25.170845 9 TGGGATG 135 1.3318346E-5 24.629536 6 TCCTAAG 175 1.466833E-7 24.428478 2 ACATGGG 470 0.0 24.255228 3 GTACATG 535 0.0 23.980444 1 AGAGTAC 1010 0.0 23.749912 10-11 >>END_MODULE