FastQCFastQC Report
Thu 26 May 2016
SRR937225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161473
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.39882828708205087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.30035981247638927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.19445975488162107No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2050.1269562093972367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGAC405.355697E-447.53343
GTATATG958.5699867E-735.0355031
GTATCAA8250.031.6987881
ACACGTT300.003945186731.66925612-13
GGTATCA4900.030.0815011
TTAGATG650.005809348529.2513283
AGATCGA650.005827023629.233166
TCAACGC9200.028.9333784
CAACGCA9150.028.5719935
TATCAAC9250.028.2631052
ATCAACG9550.027.8729923
AACGCAG9550.027.855686
CAGGGAC700.00834185927.1619454
TTAGTAC700.00834185927.1619453
ACGTTTT458.9292484E-426.39104514-15
ACGCAGA10300.025.8273537
GAGTACT5300.025.54453812-13
CGCAGAG10550.025.215338
GCAGAGT10950.024.2942249
GTACATG5900.024.177041
Produced by FastQC (version 0.11.5)