##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937225_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 161473 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.727929746768808 33.0 31.0 34.0 30.0 34.0 2 31.845070073634602 33.0 31.0 34.0 30.0 34.0 3 31.887931728524272 33.0 31.0 34.0 30.0 34.0 4 35.498987446817736 37.0 35.0 37.0 33.0 37.0 5 35.465477200522685 37.0 35.0 37.0 33.0 37.0 6 35.55555417933648 37.0 35.0 37.0 33.0 37.0 7 35.50496987112397 37.0 35.0 37.0 33.0 37.0 8 35.4840437720238 37.0 35.0 37.0 33.0 37.0 9 37.12691905148229 39.0 37.0 39.0 34.0 39.0 10-11 37.0392697231116 39.0 37.0 39.0 33.0 39.0 12-13 36.92348256364841 39.0 37.0 39.0 33.0 39.0 14-15 38.11832938014405 40.0 38.0 41.0 33.0 41.0 16-17 38.049305456639814 40.0 38.0 41.0 33.0 41.0 18-19 38.02287379314188 40.0 38.0 41.0 33.0 41.0 20-21 37.951638973698394 40.0 38.0 41.0 33.0 41.0 22-23 37.827311686783545 40.0 38.0 41.0 33.0 41.0 24-25 37.692363429180105 40.0 37.5 41.0 32.0 41.0 26-27 37.52070934459631 40.0 37.0 41.0 32.0 41.0 28-29 37.34966217262328 40.0 37.0 41.0 32.0 41.0 30-31 37.31071138828163 40.0 37.0 41.0 32.0 41.0 32-33 37.157722962972144 39.0 36.5 41.0 31.5 41.0 34-35 36.98303431533445 39.0 36.0 41.0 31.0 41.0 36-37 36.986381624172466 39.5 36.0 41.0 31.0 41.0 38-39 36.97000736965313 40.0 36.5 41.0 31.0 41.0 40-41 36.82932131068352 40.0 36.0 41.0 30.5 41.0 42-43 36.616214475485066 39.0 36.0 41.0 30.0 41.0 44-45 36.39437553027441 39.0 36.0 41.0 30.0 41.0 46-47 36.0831346417048 39.0 35.5 41.0 29.5 41.0 48-49 35.772955850204056 39.0 35.0 41.0 28.0 41.0 50-51 34.867067559282354 38.0 34.0 40.0 26.0 40.5 52-53 35.01204535742818 38.5 34.5 40.0 27.0 40.5 54-55 35.376276529203025 39.0 35.0 40.0 27.0 41.0 56-57 35.17399503322537 39.0 35.0 40.0 26.0 41.0 58-59 34.96236212865309 38.5 35.0 40.0 26.0 41.0 60-61 34.689951261201564 38.0 34.0 40.0 25.5 41.0 62-63 34.39113659868833 38.0 34.0 40.0 25.0 41.0 64-65 34.06883194094368 37.5 34.0 40.0 24.0 41.0 66-67 33.689328866126225 37.0 33.5 40.0 22.5 41.0 68-69 33.212948914059936 36.0 33.0 39.0 21.5 41.0 70-71 32.8036575774278 36.0 32.5 39.0 20.5 40.5 72-73 32.4074458268565 35.0 32.0 38.5 19.5 40.0 74-75 31.952858991905767 35.0 32.0 37.5 18.0 40.0 76-77 31.506288977104532 35.0 31.0 37.0 15.5 39.0 78-79 31.04776649966248 35.0 31.0 36.5 11.5 39.0 80-81 30.600654598601622 35.0 31.0 36.0 7.5 38.5 82-83 30.142029936893472 34.0 30.0 35.5 6.0 37.0 84-85 29.678429211075535 34.0 30.0 35.0 2.0 37.0 86-87 29.26757414552278 34.0 29.0 35.0 2.0 36.0 88-89 28.887745938949546 34.0 29.0 35.0 2.0 36.0 90-91 28.465950963938244 34.0 29.0 35.0 2.0 36.0 92-93 28.018290983631935 34.0 28.5 35.0 2.0 35.0 94-95 27.498863587101248 33.5 27.0 35.0 2.0 35.0 96-97 26.89530757464096 33.0 25.5 35.0 2.0 35.0 98-99 26.217838276368184 33.0 25.0 35.0 2.0 35.0 100-101 24.393911056337593 31.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3634240991634705 1101 2 0.4184265007048076 1101 3 0.48495620257617134 1101 4 0.20131117682284128 1101 5 0.32731462396431255 1101 6 0.16839798712986465 1101 7 0.38901493922122654 1101 8 0.22146302353357328 1101 9 0.3267632000546925 1101 10-11 0.525248885212271 1101 12-13 0.42021490713484155 1101 14-15 0.725684958348161 1101 16-17 0.8753326154698584 1101 18-19 0.7789326072262597 1101 20-21 0.49863088286925716 1101 22-23 0.400667873434152 1101 24-25 0.41054519913380005 1101 26-27 0.6178555372797447 1101 28-29 0.6920316159128532 1101 30-31 0.5795326462044912 1101 32-33 0.7316010000765729 1101 34-35 0.7944617245654868 1101 36-37 0.6950961731081122 1101 38-39 0.8546278450136953 1101 40-41 0.5429980715852096 1101 42-43 0.6441211647339102 1101 44-45 0.6398053691006638 1101 46-47 0.5370614730310734 1101 48-49 0.7139596604125771 1101 50-51 0.7690670626170899 1101 52-53 0.06447163755017726 1101 54-55 0.5255914411904783 1101 56-57 0.6876936842176775 1101 58-59 0.4546632645325701 1101 60-61 0.6747554517526382 1101 62-63 0.532160530072602 1101 64-65 0.6667481332126428 1101 66-67 0.40007357712721614 1101 68-69 0.558097436760356 1101 70-71 0.25467003349394446 1101 72-73 0.3962443419262911 1101 74-75 0.4950440218705161 1101 76-77 -0.022434633079349453 1101 78-79 0.174072555534881 1101 80-81 0.513070716108281 1101 82-83 0.9423453523814125 1101 84-85 1.0294107591532544 1101 86-87 -0.03985925886646058 1101 88-89 0.07077810004087226 1101 90-91 0.0039479385291585345 1101 92-93 0.043829992057137446 1101 94-95 0.37318670207534055 1101 96-97 0.6490535807869691 1101 98-99 0.7305227295210983 1101 100-101 1.0239799525717501 1102 1 -0.023453970450024286 1102 2 -0.21789992822251847 1102 3 0.13404154672877766 1102 4 -0.449998524935296 1102 5 0.07368447235209885 1102 6 0.14195482391539826 1102 7 -0.04660325424449496 1102 8 0.2631563824915091 1102 9 0.19605994715413289 1102 10-11 0.05318681673765013 1102 12-13 0.002115754170219475 1102 14-15 -0.05010869244548388 1102 16-17 -0.0024077931001471597 1102 18-19 0.195287863828149 1102 20-21 0.07549578428572801 1102 22-23 0.24745412156569557 1102 24-25 0.44495156257604407 1102 26-27 0.0885918886214796 1102 28-29 0.013113187554964156 1102 30-31 -0.14582147152631109 1102 32-33 -0.08637536834822157 1102 34-35 0.19013935286643147 1102 36-37 0.14220094924876037 1102 38-39 0.485971712904643 1102 40-41 0.22406967810524492 1102 42-43 -0.024351680308441814 1102 44-45 -0.12931520887641312 1102 46-47 -0.04417206672264484 1102 48-49 0.2544518662635227 1102 50-51 0.39962795663090134 1102 52-53 0.5724259403475287 1102 54-55 0.4324442421015746 1102 56-57 0.6953632692415255 1102 58-59 0.22282292002406479 1102 60-61 0.5294426180990044 1102 62-63 0.6276922405146195 1102 64-65 0.6211754945906733 1102 66-67 0.9087841615104395 1102 68-69 0.6623753213073016 1102 70-71 0.8288212186316741 1102 72-73 0.4663560936523936 1102 74-75 0.01879126366708661 1102 76-77 0.2964848629701926 1102 78-79 0.1489995966054174 1102 80-81 0.19105491427184873 1102 82-83 0.4545081397684143 1102 84-85 0.8057560506330752 1102 86-87 0.7368419298302449 1102 88-89 0.5948990339151692 1102 90-91 0.11759481731056809 1102 92-93 0.11440441016518932 1102 94-95 -0.13670724055402772 1102 96-97 0.0634691975568451 1102 98-99 -0.026657496829528782 1102 100-101 0.3963463131668128 1103 1 0.1077280408358341 1103 2 0.07413774852131638 1103 3 0.2599239249123144 1103 4 0.15257190439935897 1103 5 0.060682133647610215 1103 6 -0.04535609504527116 1103 7 0.1299582064802891 1103 8 0.12639581004530953 1103 9 -0.17287604958455915 1103 10-11 0.06037457823270387 1103 12-13 0.24670347704446982 1103 14-15 -0.03336228096222982 1103 16-17 0.08698864949431595 1103 18-19 -0.11507324072696434 1103 20-21 0.14696573515147549 1103 22-23 0.2340012067674877 1103 24-25 0.4380856411479286 1103 26-27 0.27894956021588513 1103 28-29 0.23597670591057351 1103 30-31 0.11048122978464647 1103 32-33 -0.2732786232241011 1103 34-35 0.1976382264262 1103 36-37 0.1472967503086906 1103 38-39 0.08461569342259168 1103 40-41 0.3535566650385462 1103 42-43 -0.23832168481420268 1103 44-45 -0.0491233921965204 1103 46-47 -0.35131477060832594 1103 48-49 -0.19937481213242592 1103 50-51 -0.36408779422198023 1103 52-53 -0.10566962469634689 1103 54-55 -0.009334889788483736 1103 56-57 -0.05904057567447296 1103 58-59 0.0851504673881962 1103 60-61 0.16643920659429057 1103 62-63 0.21662737901313278 1103 64-65 0.03731470114762914 1103 66-67 0.24416296726923292 1103 68-69 0.22332110198401978 1103 70-71 0.09694617840166586 1103 72-73 0.3296384332151163 1103 74-75 0.38016401883261963 1103 76-77 0.7859499647789256 1103 78-79 0.420578973093928 1103 80-81 0.39835210138966204 1103 82-83 0.418773164335537 1103 84-85 0.5934059744642575 1103 86-87 0.5965518446499054 1103 88-89 1.21865726765688 1103 90-91 0.5751012004718952 1103 92-93 0.4596422499746602 1103 94-95 0.7959101774303932 1103 96-97 1.1511706157987405 1103 98-99 1.4916015629683557 1103 100-101 0.9506621884645128 1104 1 -0.16846151940556453 1104 2 -0.07832312041667322 1104 3 0.052715634657207744 1104 4 -0.39526296372115155 1104 5 -0.05453510085023794 1104 6 -0.08329699572089311 1104 7 0.1298620318271162 1104 8 0.047797074742000234 1104 9 -0.03171570239405952 1104 10-11 -0.04047656744784689 1104 12-13 0.010318201383860526 1104 14-15 -0.09282226028468443 1104 16-17 -0.04963946716554091 1104 18-19 0.1583993855623831 1104 20-21 0.14530672238428366 1104 22-23 0.3646544730991508 1104 24-25 0.23122598401056393 1104 26-27 0.23354310208791418 1104 28-29 -0.0037987262742689154 1104 30-31 -0.41552199206622475 1104 32-33 -0.3909362895461399 1104 34-35 -0.28559131842725805 1104 36-37 -0.05644925243229437 1104 38-39 0.057686790534951626 1104 40-41 -0.07438447607371756 1104 42-43 0.11697152765964347 1104 44-45 0.34492820550490677 1104 46-47 -0.4363572076740354 1104 48-49 -0.1164722610997515 1104 50-51 -0.11681073908985695 1104 52-53 -0.5368085730265122 1104 54-55 -0.7782392200522423 1104 56-57 -0.7038074723588537 1104 58-59 -0.6391889329628455 1104 60-61 -0.21277745085563993 1104 62-63 -0.14075763216717263 1104 64-65 -0.1342608801079308 1104 66-67 -0.1733191803107701 1104 68-69 -0.12775244758199022 1104 70-71 -0.48456184016004045 1104 72-73 -0.4126014744572153 1104 74-75 0.1058859298229784 1104 76-77 -0.057730158324623915 1104 78-79 -0.2687167880082306 1104 80-81 -0.06601518961079478 1104 82-83 -0.6683370277733296 1104 84-85 -0.47247366295729876 1104 86-87 -0.4697966442058572 1104 88-89 -0.46620243756780866 1104 90-91 -0.3993283636164904 1104 92-93 0.13404296166709173 1104 94-95 0.20572239642007872 1104 96-97 0.3477155413836037 1104 98-99 -0.34877255643247906 1104 100-101 0.4399387146360425 1105 1 -0.044200976183123686 1105 2 -0.190625136827844 1105 3 -0.3039728711630332 1105 4 0.06081489392149564 1105 5 -0.18700122895914006 1105 6 -0.12957267792940996 1105 7 -0.5377481710861858 1105 8 -0.11202100298842765 1105 9 -0.18323278146027633 1105 10-11 -0.4067382556356307 1105 12-13 -0.5764447271830164 1105 14-15 -0.005804894977124775 1105 16-17 0.07171095583554177 1105 18-19 -0.17553293426460215 1105 20-21 0.19073958288934278 1105 22-23 -0.09511271728314341 1105 24-25 -0.16819199194515733 1105 26-27 -0.1889809103794846 1105 28-29 -0.2678086493071561 1105 30-31 -0.01952300981319155 1105 32-33 -0.30341005075726457 1105 34-35 -0.34811914744576455 1105 36-37 -0.09436032679142414 1105 38-39 -0.03139787329050847 1105 40-41 0.21642081621048703 1105 42-43 -0.24599838519576878 1105 44-45 -0.06923119911311915 1105 46-47 0.0758398535815985 1105 48-49 -0.2693887422396273 1105 50-51 0.2682633656108635 1105 52-53 0.4879194977068977 1105 54-55 0.15641824448563568 1105 56-57 0.06739028859782792 1105 58-59 0.09633997741182299 1105 60-61 0.6058886645314274 1105 62-63 0.16213340529863984 1105 64-65 -0.10972363693002052 1105 66-67 -0.025157485762079546 1105 68-69 0.41981132062614535 1105 70-71 0.627183595880922 1105 72-73 -0.17257221423203362 1105 74-75 0.021842293921952916 1105 76-77 -0.126714319637518 1105 78-79 -0.33205563413761396 1105 80-81 0.10634979989369242 1105 82-83 0.7402406708463509 1105 84-85 0.5594740502924971 1105 86-87 -0.04134107522169472 1105 88-89 0.06382109783070433 1105 90-91 0.4472621840585944 1105 92-93 -0.08561545700856499 1105 94-95 -0.274542853882064 1105 96-97 0.13802786248276888 1105 98-99 0.5099832479056658 1105 100-101 0.6216542543599779 1106 1 -0.19985910885657887 1106 2 -0.13023682427430927 1106 3 0.2811132618911216 1106 4 -0.5658149718822543 1106 5 -0.266043772025661 1106 6 0.22395354268542178 1106 7 -0.07715047431260302 1106 8 -0.23655702659173983 1106 9 0.08344657515912246 1106 10-11 -0.24584551746107763 1106 12-13 -0.2676505899411765 1106 14-15 -0.7907101962048841 1106 16-17 -0.7636607011550325 1106 18-19 0.046580894611388146 1106 20-21 -0.014004873187552391 1106 22-23 0.20768541729380274 1106 24-25 0.23166048735435396 1106 26-27 0.3859215356089081 1106 28-29 0.5169063026296499 1106 30-31 0.13884760025628395 1106 32-33 -0.06309407093429087 1106 34-35 0.31691368780692386 1106 36-37 -0.34928561469472896 1106 38-39 -0.0975442518952434 1106 40-41 -0.3518431982560557 1106 42-43 -0.8695588686829723 1106 44-45 0.05511447519923962 1106 46-47 -0.19581237826383102 1106 48-49 -0.4122934724194991 1106 50-51 -0.6484350259376299 1106 52-53 -0.4925459063094735 1106 54-55 0.012537974189640977 1106 56-57 -0.31126203842225664 1106 58-59 0.03183536144150878 1106 60-61 0.23615896052456975 1106 62-63 0.5331003796966627 1106 64-65 0.2358792944490702 1106 66-67 0.4847644710286403 1106 68-69 0.07397045263336821 1106 70-71 0.10617379289244155 1106 72-73 0.793069738751683 1106 74-75 0.415365659297418 1106 76-77 0.49510922656977385 1106 78-79 0.23192552128258015 1106 80-81 0.1847499141032678 1106 82-83 0.3018900474524209 1106 84-85 -0.29483736114749703 1106 86-87 0.005984517240474219 1106 88-89 0.14962104072591842 1106 90-91 0.6222645634247321 1106 92-93 0.16162447827267457 1106 94-95 1.0477898766785145 1106 96-97 0.5944378748554762 1106 98-99 0.9188059375411513 1106 100-101 1.602405182312772 1107 1 -0.33760914432260236 1107 2 -0.6346582552101374 1107 3 -0.5273229229105034 1107 4 -0.48953937723526053 1107 5 -0.23265842185779206 1107 6 -0.4966214797763939 1107 7 -0.7071216286134501 1107 8 -0.2711245775445903 1107 9 -0.5914009209396198 1107 10-11 -0.7059580629758031 1107 12-13 -0.7923112983158873 1107 14-15 -0.5155174974769281 1107 16-17 -0.5541747220563451 1107 18-19 -0.303090453570384 1107 20-21 -0.5646739988318572 1107 22-23 -0.4263761517230762 1107 24-25 -0.15515560949153695 1107 26-27 -0.45173865259746293 1107 28-29 -0.5758255269442927 1107 30-31 -0.13714899500545386 1107 32-33 -0.05673383071108873 1107 34-35 -0.31691144105295876 1107 36-37 -0.0742584240766746 1107 38-39 -0.21597712653467482 1107 40-41 -0.20808758280232809 1107 42-43 0.12656351274229394 1107 44-45 -0.07062440029137207 1107 46-47 0.3617844439804543 1107 48-49 0.10243674936136671 1107 50-51 0.4474887457557557 1107 52-53 -0.011224678712174807 1107 54-55 0.5154461881206913 1107 56-57 0.21741815262139141 1107 58-59 -0.035715591412319725 1107 60-61 0.11096389739504531 1107 62-63 0.4612935040168935 1107 64-65 0.7843731706489976 1107 66-67 0.7857055028534816 1107 68-69 0.9728922855174673 1107 70-71 0.7189557477796598 1107 72-73 0.7539861808002044 1107 74-75 0.4750904429546772 1107 76-77 0.7421655466151726 1107 78-79 0.6762438643099635 1107 80-81 0.7974372929276861 1107 82-83 1.0937754212643078 1107 84-85 0.7629013251909917 1107 86-87 0.6750628735085016 1107 88-89 0.5555415022571033 1107 90-91 0.5590168214282301 1107 92-93 0.12648947094303153 1107 94-95 0.4736658874601858 1107 96-97 0.3119391752763363 1107 98-99 -0.11954465592364372 1107 100-101 0.47789579563853835 1108 1 7.088002779589431E-4 1108 2 6.622318767064428E-4 1108 3 -0.36450343943666397 1108 4 -0.13168443276851605 1108 5 0.22371786611223143 1108 6 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0.21926119229875596 2316 82-83 -0.07773401269795599 2316 84-85 0.391077425819919 2316 86-87 -0.14644656045376436 2316 88-89 -0.1093012650384928 2316 90-91 -0.2704171408359173 2316 92-93 -0.08426524603810392 2316 94-95 6.470195356591546E-4 2316 96-97 0.03659326332665103 2316 98-99 0.07837987237824251 2316 100-101 -0.08927401568221427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 751.0 3 145.0 4 113.0 5 128.0 6 147.0 7 190.0 8 193.0 9 230.0 10 317.0 11 358.0 12 543.0 13 674.0 14 734.0 15 976.0 16 1218.0 17 1535.0 18 1349.0 19 941.0 20 909.0 21 935.0 22 982.0 23 1131.0 24 1216.0 25 1411.0 26 1643.0 27 1993.0 28 2335.0 29 2900.0 30 3484.0 31 4424.0 32 5531.0 33 7380.0 34 10280.0 35 14761.0 36 22371.0 37 32890.0 38 29908.0 39 4447.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.98958145772667 19.94796937768147 15.958127145952725 22.104322018639134 2 14.61107283189631 20.53545379491859 41.602264474198776 23.251208898986324 3 18.603380739839466 25.29781673484844 30.552675849995964 25.546126675316128 4 12.235472316545511 17.531302571870206 38.8213968675701 31.411828244014178 5 13.088816749117047 37.887242639984606 34.95502988696953 14.068910723928818 6 30.054536317216474 37.86024060504911 18.157677584270708 13.927545493463708 7 26.968585770216357 33.71181802952181 21.481178142198562 17.838418058063272 8 25.525512483248125 35.39174566933042 20.47513277411029 18.607609073311163 9 25.60430833374696 17.707971410135503 20.949148756638706 35.73857149947883 10-11 24.085467062542854 27.653006456256612 27.889105028186012 20.37242145301452 12-13 25.117737942331676 24.91328654840131 28.291853589223333 21.67712192004368 14-15 22.151392197736747 26.371414036132617 26.8181084971213 24.65908526900933 16-17 21.20273879955698 29.60714296794289 27.46710679394784 21.723011438552295 18-19 21.076989887710155 28.05974316024567 30.09398846082263 20.76927849122154 20-21 21.723120639539392 27.201829028065156 30.432719004085534 20.64233132830992 22-23 21.402137328804304 27.090409939044253 30.31315434367875 21.194298388472692 24-25 21.107829938767534 27.419675039860042 30.40250885606338 21.069986165309047 26-27 21.055782701220384 27.47163091966075 30.22912414148245 21.243462237636418 28-29 20.89004975741708 27.913166482609718 30.085865667382212 21.110918092590982 30-31 21.158123648592053 27.534132260376175 29.968450344192167 21.339293746839605 32-33 20.938926064148262 27.56656646408994 29.844236180265366 21.65027129149643 34-35 21.627381096397762 27.596058104800814 29.76490695849347 21.011653840307957 36-37 21.496593996997255 27.44438102564739 29.840370751802265 21.218654225553088 38-39 21.087340213348718 27.96084160765301 29.646414358077195 21.30540382092108 40-41 21.34705639670324 27.809366052789127 29.80268443468362 21.04089311582402 42-43 21.172850611363735 27.777502062693475 29.571580116255262 21.478067209687527 44-45 21.267896198172764 27.803750639842406 29.536366315593543 21.39198684639129 46-47 21.597292661213526 28.185109016294284 28.893272908310923 21.32432541418127 48-49 21.33081027422757 28.280804069983866 28.855937461223473 21.53244819456508 50-51 21.636084441066526 28.283414248266663 28.727955204665662 21.352546106001146 52-53 21.69061534452593 28.31620940823189 28.70118750697986 21.291987740262318 54-55 21.502340717944264 28.44197222161899 28.734212951041922 21.321474109394824 56-57 21.463155903089028 28.483338968172976 28.523351499229214 21.530153629508778 58-59 21.35821154752748 28.420961218767832 28.664429446939433 21.556397786765253 60-61 21.316600807677275 28.442708882589034 28.936496219052994 21.304194090680696 62-63 21.094651431123683 28.527561692782967 29.08555725115073 21.292229624942618 64-65 21.238729447816606 28.403497399282283 28.69474056406264 21.663032588838472 66-67 21.116532712106782 28.352870742897203 28.645078837247535 21.885517707748477 68-69 21.464594770633667 28.427157966564316 28.608914115567135 21.499333147234886 70-71 21.436215255483283 28.329750704893776 28.522064958792022 21.711969080830922 72-73 21.337481814205546 28.39372516417623 28.408615025731542 21.860177995886676 74-75 21.378985179073272 28.5890651463171 28.34835691819022 21.683592756419404 76-77 21.383060563572517 28.438574069708288 28.540940280173217 21.637425086545978 78-79 21.415787008458988 28.45439684346685 28.415312411788605 21.71450373628555 80-81 21.536132900340252 28.486354204468267 28.194176925438963 21.783335969752518 82-83 21.591406121556354 28.702201514413606 28.33957359423518 21.36681876979486 84-85 21.512073323924877 28.74521176780757 28.23126192211659 21.511452986150957 86-87 21.573705611781236 28.72327557062259 28.136446957454925 21.56657186014125 88-89 21.336956285729354 28.48019008092708 28.242278737798127 21.940574895545446 90-91 21.379674002388374 28.50881682408226 28.385675956512973 21.725833217016394 92-93 21.52612983177767 28.44734793142063 28.334434549013405 21.692087687788295 94-95 21.21334317672823 28.35425054748714 28.31609705256497 22.11630922321966 96-97 21.37123289370836 28.294085534559983 28.389930396158764 21.9447511755729 98-99 21.307397148094957 28.33200467769503 28.1071154937791 22.25348268043092 100-101 21.611830678378244 28.123604287628407 28.202074338742495 22.062490695250855 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 194.0 1 111.5 2 26.5 3 29.0 4 28.5 5 22.0 6 47.5 7 96.0 8 145.0 9 179.0 10 170.0 11 160.5 12 163.5 13 179.5 14 203.5 15 204.5 16 216.5 17 239.0 18 257.0 19 284.5 20 328.5 21 372.5 22 451.5 23 576.5 24 704.5 25 854.5 26 1101.5 27 1441.0 28 1731.5 29 2016.5 30 2342.0 31 2750.5 32 3261.0 33 3622.5 34 4004.5 35 4378.5 36 4778.5 37 5143.5 38 5360.5 39 5589.0 40 5647.5 41 5800.0 42 5961.5 43 5957.5 44 5929.0 45 6021.0 46 6051.0 47 6051.0 48 6168.5 49 5892.5 50 5448.5 51 5171.0 52 4868.5 53 4622.0 54 4482.0 55 4085.0 56 3583.0 57 3162.0 58 2823.0 59 2413.0 60 1857.0 61 1492.0 62 1244.0 63 872.0 64 544.0 65 416.0 66 324.0 67 245.5 68 168.5 69 131.0 70 104.5 71 73.5 72 55.0 73 37.5 74 23.5 75 18.5 76 17.5 77 11.0 78 5.0 79 5.0 80 5.5 81 3.0 82 1.5 83 1.5 84 2.0 85 1.5 86 1.0 87 1.0 88 1.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2563896131241756 2 0.23285626699200485 3 0.23781065565140921 4 0.23781065565140921 5 0.22666328116774942 6 0.18331238039796127 7 0.1882667690573656 8 0.18393167898038681 9 0.18393167898038681 10-11 0.1929115084255572 12-13 0.1919825605519189 14-15 0.17897729032098245 16-17 0.19043431409585504 18-19 0.1752614988264292 20-21 0.18238343252432296 22-23 0.17990623819462076 24-25 0.17588079740885473 26-27 0.1820737832331102 28-29 0.18021588748583353 30-31 0.18486062685402513 32-33 0.18547992543645067 34-35 0.17433255095279088 36-37 0.17773869315613136 38-39 0.17433255095279088 40-41 0.1761904467000675 42-43 0.17092640874945036 44-45 0.1860992240188762 46-47 0.17433255095279088 48-49 0.18145448465068464 50-51 0.1830027311067485 52-53 0.18269308181553573 54-55 0.18857641834857838 56-57 0.1693781622933865 58-59 0.1616369300130672 60-61 0.1672106172548971 62-63 0.16782991583732265 64-65 0.16566237079883323 66-67 0.1774290438649186 68-69 0.165972020090046 70-71 0.17247465520551425 72-73 0.17928693961219522 74-75 0.17464220024400362 76-77 0.17773869315613136 78-79 0.17557114811764196 80-81 0.16690096796368434 82-83 0.1786676410297697 84-85 0.16752026654610988 86-87 0.16566237079883323 88-89 0.17247465520551425 90-91 0.1706167594582376 92-93 0.17804834244734413 94-95 0.17464220024400362 96-97 0.17030711016702482 98-99 0.17495184953521642 100-101 0.1641141243427694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 161473.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.84258128863668 #Duplication Level Percentage of deduplicated Percentage of total 1 89.75942743322122 71.66624381258451 2 6.886241171009094 10.996305409389006 3 1.5144789704057482 3.627597309136553 4 0.6253143118859554 1.9970683511080924 5 0.3336277305684016 1.3318849599025493 6 0.21980894700863793 1.0530068231704084 7 0.13322890292651685 0.7446137658334414 8 0.08649038431806139 0.5524492432480195 9 0.067599695478777 0.4857600763216205 >10 0.3449535049256548 4.70912144827842 >50 0.020876134941053975 1.148507672848201 >100 0.0069587116470179915 1.176287747865262 >500 9.941016638597131E-4 0.5111533803139195 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 644 0.39882828708205087 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 485 0.30035981247638927 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 314 0.19445975488162107 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 205 0.1269562093972367 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 6.192985824255449E-4 0.0 26-27 0.0 0.0 0.0 0.0012385971648510897 0.0 28-29 3.0964929121277243E-4 0.0 0.0 0.0024771943297021795 0.0 30-31 6.192985824255449E-4 0.0 0.0 0.008670180153957627 0.0 32-33 6.192985824255449E-4 0.0 0.0 0.014553516687000303 0.0 34-35 6.192985824255449E-4 0.0 0.0 0.019817554637617432 0.0 36-37 6.192985824255449E-4 0.0 0.0 0.022604398258532388 0.0 38-39 6.192985824255449E-4 0.0 0.0 0.02724913762672397 0.0 40-41 6.192985824255449E-4 0.0 0.0 0.03839651211038378 0.0 42-43 6.192985824255449E-4 0.0 0.0 0.052330730214958536 0.0 44-45 6.192985824255449E-4 0.0 0.0 0.07493512847349093 0.0 46-47 6.192985824255449E-4 0.0 0.0 0.10063601964415103 0.0 48-49 6.192985824255449E-4 0.0 0.0 0.16813956512853542 0.0 50-51 6.192985824255449E-4 0.0 0.0 0.22604398258532388 0.0 52-53 6.192985824255449E-4 0.0 0.0 0.28116155642119733 0.0 54-55 6.192985824255449E-4 0.0 0.0 0.3790107324444334 0.0 56-57 6.192985824255449E-4 0.0 0.0 0.46509323540158415 0.0 58-59 6.192985824255449E-4 0.0 0.0 0.5483888947378199 0.0 60-61 6.192985824255449E-4 0.0 0.0 0.6592433409919924 0.0 62-63 6.192985824255449E-4 0.0 0.0 0.7973469248728889 0.0 64-65 6.192985824255449E-4 0.0 0.0 0.8846680249948908 0.0 66-67 6.192985824255449E-4 0.0 0.0 1.012243532974553 0.0 68-69 6.192985824255449E-4 0.0 0.0 1.2045357428176846 0.0 70-71 6.192985824255449E-4 0.0 0.0 1.3621472320449857 0.0 72-73 6.192985824255449E-4 0.0 0.0 1.6008868355700334 3.0964929121277243E-4 74-75 6.192985824255449E-4 0.0 0.0 1.92663788992587 6.192985824255449E-4 76-77 6.192985824255449E-4 0.0 0.0 2.152372223219981 6.192985824255449E-4 78-79 6.192985824255449E-4 0.0 0.0 2.3855381395031987 6.192985824255449E-4 80-81 6.192985824255449E-4 0.0 0.0 2.6933295349686945 6.192985824255449E-4 82-83 6.192985824255449E-4 0.0 0.0 3.0537613099403615 6.192985824255449E-4 84-85 6.192985824255449E-4 0.0 0.0 3.43555888600571 6.192985824255449E-4 86-87 6.192985824255449E-4 0.0 0.0 3.8830021118081666 6.192985824255449E-4 88-89 6.192985824255449E-4 0.0 0.0 4.4196243334799 6.192985824255449E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGAC 40 5.355697E-4 47.5334 3 GTATATG 95 8.5699867E-7 35.035503 1 GTATCAA 825 0.0 31.698788 1 ACACGTT 30 0.0039451867 31.669256 12-13 GGTATCA 490 0.0 30.081501 1 TTAGATG 65 0.0058093485 29.251328 3 AGATCGA 65 0.0058270236 29.23316 6 TCAACGC 920 0.0 28.933378 4 CAACGCA 915 0.0 28.571993 5 TATCAAC 925 0.0 28.263105 2 ATCAACG 955 0.0 27.872992 3 AACGCAG 955 0.0 27.85568 6 CAGGGAC 70 0.008341859 27.161945 4 TTAGTAC 70 0.008341859 27.161945 3 ACGTTTT 45 8.9292484E-4 26.391045 14-15 ACGCAGA 1030 0.0 25.827353 7 GAGTACT 530 0.0 25.544538 12-13 CGCAGAG 1055 0.0 25.21533 8 GCAGAGT 1095 0.0 24.294224 9 GTACATG 590 0.0 24.17704 1 >>END_MODULE