FastQCFastQC Report
Thu 26 May 2016
SRR937225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161473
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9430.5839985632272887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7770.4811949985446483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.3177001727843045No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.12881410514451333No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC1640.10156496751778936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4600.051.658561
GTATCAA7900.042.7131271
CTATAGG500.001607395438.02071
TAGGACA801.0724374E-535.622334
TATAAGG702.0464529E-433.9260252
ATCAACG10200.032.5955923
TATCAAC10300.032.2791332
CAACGCA10400.031.9687565
TCAACGC10400.031.9687564
AACGCAG10750.030.9279146
CAGGGGT804.478705E-429.6852744
GTAAGGT650.00583153629.2285774
GGTCTAC1004.9088652E-528.5155261
TGGTATC2201.8189894E-1228.0660762
ACGCAGA11750.027.8915277
CGCAGAG11700.027.6047678
TCCCTAT700.00834837527.1576421
GTGTGCA1701.11118425E-725.1451749
GTGGTAT2900.024.582351
GCAGAGT12950.024.5734489
Produced by FastQC (version 0.11.5)