##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937225_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 161473 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13339691465446 33.0 31.0 34.0 31.0 34.0 2 32.35985582729001 34.0 31.0 34.0 31.0 34.0 3 32.438036080335415 34.0 31.0 34.0 31.0 34.0 4 36.06431415778489 37.0 35.0 37.0 35.0 37.0 5 35.9528404129483 37.0 35.0 37.0 35.0 37.0 6 36.02615917212165 37.0 35.0 37.0 35.0 37.0 7 35.986499290903126 37.0 35.0 37.0 35.0 37.0 8 35.96121332978269 37.0 35.0 37.0 35.0 37.0 9 37.65786849813901 39.0 37.0 39.0 35.0 39.0 10-11 37.58580072210215 39.0 37.0 39.0 35.0 39.0 12-13 37.51259653316654 39.0 37.0 39.0 35.0 39.0 14-15 38.71231413301295 40.0 38.0 41.0 34.5 41.0 16-17 38.68144209867904 40.0 38.0 41.0 34.5 41.0 18-19 38.704805137701044 40.0 38.0 41.0 34.5 41.0 20-21 38.61357626352394 40.0 38.0 41.0 34.0 41.0 22-23 38.49552866423489 40.0 38.0 41.0 34.0 41.0 24-25 38.36663714676757 40.0 38.0 41.0 34.0 41.0 26-27 38.29852668867241 40.0 38.0 41.0 34.0 41.0 28-29 38.200132529896635 40.0 38.0 41.0 34.0 41.0 30-31 38.255621682881966 40.0 38.0 41.0 34.0 41.0 32-33 38.15682188353471 40.0 38.0 41.0 34.0 41.0 34-35 38.0482309735993 40.0 37.0 41.0 33.5 41.0 36-37 37.88237662023992 40.0 37.0 41.0 33.0 41.0 38-39 37.74238107918971 40.0 37.0 41.0 33.0 41.0 40-41 37.56680373808624 40.0 37.0 41.0 33.0 41.0 42-43 37.35971648510896 40.0 37.0 41.0 32.5 41.0 44-45 37.05120051030203 40.0 36.0 41.0 31.5 41.0 46-47 36.71131396580233 40.0 36.0 41.0 31.0 41.0 48-49 36.391641327033 39.0 36.0 41.0 30.0 41.0 50-51 36.14421915738235 39.0 35.5 41.0 30.0 41.0 52-53 35.93596762307011 39.0 35.0 40.5 29.0 41.0 54-55 35.73100766072346 39.0 35.0 40.0 28.5 41.0 56-57 35.47951360289336 38.5 35.0 40.0 28.0 41.0 58-59 35.22987434431762 38.0 35.0 40.0 27.5 41.0 60-61 34.912424987459204 38.0 34.0 40.0 26.5 41.0 62-63 34.56107212970589 38.0 34.0 40.0 26.0 41.0 64-65 34.20747121809838 37.0 34.0 40.0 26.0 41.0 66-67 33.79498430078094 36.5 33.0 39.0 25.5 41.0 68-69 33.392492862583836 36.0 33.0 39.0 24.0 40.0 70-71 32.97044087866083 35.5 32.5 39.0 23.5 40.0 72-73 32.54357075176655 35.0 32.0 38.0 22.0 40.0 74-75 32.10325874914072 35.0 31.5 37.0 21.5 39.0 76-77 30.753029918314517 34.0 30.0 36.0 20.0 38.5 78-79 31.40655713339072 34.5 31.0 36.0 20.0 39.0 80-81 31.268657918042024 35.0 31.0 36.0 20.0 38.5 82-83 30.924247397397707 34.0 31.0 36.0 18.5 37.0 84-85 30.52467904850966 34.0 31.0 35.0 17.5 37.0 86-87 30.142931016330905 34.0 30.5 35.0 13.0 36.0 88-89 29.734546952122027 34.0 30.0 35.0 7.0 36.0 90-91 29.281898521734284 34.0 29.5 35.0 3.5 35.5 92-93 28.914589436004782 34.0 29.0 35.0 2.0 35.0 94-95 28.46688920129062 34.0 29.0 35.0 2.0 35.0 96-97 27.921955992642733 33.5 28.5 35.0 2.0 35.0 98-99 27.387235017619044 33.0 27.5 35.0 2.0 35.0 100-101 25.4387482737052 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3305125520402825 1101 2 0.288635726000777 1101 3 0.17234680480717657 1101 4 0.1984602810276357 1101 5 0.24185146356068543 1101 6 0.20480821465094579 1101 7 -0.015642881598473934 1101 8 0.19991832154456546 1101 9 0.4649948237668866 1101 10-11 0.3420807604845848 1101 12-13 0.31513985859393046 1101 14-15 0.3441801092700061 1101 16-17 0.6134118340730694 1101 18-19 0.34775524471141495 1101 20-21 0.4967765492152978 1101 22-23 0.2773580180308812 1101 24-25 0.3153112115767911 1101 26-27 0.5688192360478936 1101 28-29 0.3972541148141886 1101 30-31 0.4247758190491808 1101 32-33 0.471797211537087 1101 34-35 0.2669172605642274 1101 36-37 0.4748814546300508 1101 38-39 0.27276298655467457 1101 40-41 0.3254888993142231 1101 42-43 0.37903811986074487 1101 44-45 0.3261895319345953 1101 46-47 0.4128792526320666 1101 48-49 0.4614545562967223 1101 50-51 0.17687355474516409 1101 52-53 -0.1292594423768989 1101 54-55 0.21206234646142264 1101 56-57 0.4528062806965707 1101 58-59 0.38543516662729616 1101 60-61 0.2692212876403275 1101 62-63 0.676573134986505 1101 64-65 0.2608583804970408 1101 66-67 0.6110438938572713 1101 68-69 0.7408417311754931 1101 70-71 0.2719640651187163 1101 72-73 0.3688076693858946 1101 74-75 0.35718437101348144 1101 76-77 0.10370594395155308 1101 78-79 0.6060408914356472 1101 80-81 0.41961981871169485 1101 82-83 0.39099285595834843 1101 84-85 0.47059231618637654 1101 86-87 0.4315743038137434 1101 88-89 0.5316879481642296 1101 90-91 0.517898791436842 1101 92-93 0.38481830165143904 1101 94-95 0.5951291462705406 1101 96-97 0.7840244548229407 1101 98-99 0.6086932787636847 1101 100-101 1.2655842859887976 1102 1 0.15909465752822172 1102 2 0.13405176984731781 1102 3 0.11419319956836915 1102 4 0.010724499121451458 1102 5 -0.030374317633707903 1102 6 0.05985127821418956 1102 7 -0.12600660855412826 1102 8 -0.01865595420459698 1102 9 0.2711020200086125 1102 10-11 -0.15633549690029014 1102 12-13 0.12696810259706837 1102 14-15 0.16834019183816196 1102 16-17 -0.014817933170157005 1102 18-19 -0.27133686148831515 1102 20-21 -0.23953920279551255 1102 22-23 -0.08844203891267455 1102 24-25 -0.048486923521792846 1102 26-27 -0.06730255197972923 1102 28-29 -0.32231667314224666 1102 30-31 0.1526479095894402 1102 32-33 -0.23372453378286906 1102 34-35 -0.3322623963508562 1102 36-37 -0.07110046579133211 1102 38-39 -0.26636791244169444 1102 40-41 -0.28354911050853815 1102 42-43 -0.4925363694267446 1102 44-45 0.07141161179792732 1102 46-47 0.22432490712704833 1102 48-49 0.1218307396549747 1102 50-51 -0.011218124377904815 1102 52-53 -0.4696573354653779 1102 54-55 -0.22005915684842137 1102 56-57 -0.09037294914185168 1102 58-59 -0.3604631179481217 1102 60-61 0.11463733148686828 1102 62-63 -0.14694633259125567 1102 64-65 -0.18130832175934586 1102 66-67 -0.03361052994512903 1102 68-69 0.061095841788350924 1102 70-71 -0.2078298703927075 1102 72-73 -0.5618101681826175 1102 74-75 -0.7800851855385815 1102 76-77 -0.2202228054256281 1102 78-79 -0.33487127313122755 1102 80-81 -0.6841687071620299 1102 82-83 -0.1813610482341197 1102 84-85 -0.6955671969462323 1102 86-87 -0.6663063282597115 1102 88-89 -0.546324721776692 1102 90-91 -0.41284361015757653 1102 92-93 -0.1443207829809161 1102 94-95 -0.4240982008716081 1102 96-97 -0.6571190429349087 1102 98-99 -0.5965348513523416 1102 100-101 -0.1335366478855029 1103 1 -0.1721645953737081 1103 2 -0.17321967006514427 1103 3 -0.051318418858045334 1103 4 -0.02843270902324946 1103 5 -0.38087960653880515 1103 6 -0.2932930992398397 1103 7 -0.26152273274148286 1103 8 -0.36795970685977863 1103 9 -0.3766547547247967 1103 10-11 -0.14320442216902762 1103 12-13 -0.050043934221442044 1103 14-15 -0.11310894670325666 1103 16-17 -0.07922885856762463 1103 18-19 -0.07131325988340365 1103 20-21 -0.2715837454607808 1103 22-23 0.1351938956036065 1103 24-25 -0.014908276537433096 1103 26-27 0.09888493058726766 1103 28-29 -0.3944410321441154 1103 30-31 -0.07092365691344327 1103 32-33 -0.23167548535666782 1103 34-35 -0.11017129415590432 1103 36-37 0.0249687941910679 1103 38-39 0.05716717885360367 1103 40-41 -0.2194814811824699 1103 42-43 0.1630625749378325 1103 44-45 0.19509474952403139 1103 46-47 0.20531488717296043 1103 48-49 -0.11875543838695535 1103 50-51 -0.7994688163340484 1103 52-53 -0.5958615537158565 1103 54-55 -0.4376015860972444 1103 56-57 -0.7584700161060383 1103 58-59 -0.8768018653465788 1103 60-61 -0.8624905677557351 1103 62-63 -0.6597126552466577 1103 64-65 -0.365100455988113 1103 66-67 -0.6296905179297667 1103 68-69 -0.6152188748851088 1103 70-71 -0.855200437045788 1103 72-73 -0.8415857383759366 1103 74-75 -0.340848590177373 1103 76-77 -0.3353557253732724 1103 78-79 -0.06602753721173116 1103 80-81 -0.038675540899454575 1103 82-83 0.1824791474446421 1103 84-85 -0.006936733711096821 1103 86-87 0.038062114248926804 1103 88-89 -0.16771206702525987 1103 90-91 -0.35743347863227015 1103 92-93 -0.5092445068850537 1103 94-95 -0.6246152905791611 1103 96-97 -0.5562865499617473 1103 98-99 -0.5510052098821738 1103 100-101 -0.7118403361726671 1104 1 -0.10996363843590728 1104 2 0.07185738987571 1104 3 -0.09837186792154995 1104 4 -0.07269709314481787 1104 5 0.08946253478984545 1104 6 -0.10072739992989455 1104 7 -0.15104209991226014 1104 8 -0.012233708446665048 1104 9 -0.07245432674758945 1104 10-11 -0.12192578015513078 1104 12-13 -0.18347424366338316 1104 14-15 0.050339876359423386 1104 16-17 -0.046793956785990076 1104 18-19 -0.24071288961098958 1104 20-21 -0.3288196859286785 1104 22-23 -0.35916786535429424 1104 24-25 -0.0591005296691236 1104 26-27 -0.7278924972216316 1104 28-29 -0.10830941713125242 1104 30-31 -0.2818226494839564 1104 32-33 -0.32443593833927764 1104 34-35 -0.33656642771845213 1104 36-37 0.055203700781440546 1104 38-39 0.20414609256089733 1104 40-41 0.06977581005842382 1104 42-43 0.3780610120997139 1104 44-45 -0.06520194928100409 1104 46-47 0.11795223659952114 1104 48-49 -0.07407029015777056 1104 50-51 -0.7717584943894025 1104 52-53 0.06729874538731906 1104 54-55 0.2845378190625283 1104 56-57 0.1794853107675607 1104 58-59 0.34163481566613996 1104 60-61 0.17555852940471084 1104 62-63 0.041124064235923186 1104 64-65 -0.3191049281094891 1104 66-67 0.11716577309473308 1104 68-69 0.10474457874668985 1104 70-71 0.046581198404190616 1104 72-73 0.2715156537521679 1104 74-75 0.010729876317782328 1104 76-77 -0.1855877467240461 1104 78-79 -0.11465584622089153 1104 80-81 -0.43190965404893333 1104 82-83 -0.5916186140903079 1104 84-85 -0.28990660700099014 1104 86-87 -0.4261368711084863 1104 88-89 -0.23759497438502208 1104 90-91 -0.3626148880718105 1104 92-93 0.1279486358621753 1104 94-95 0.42414525857811114 1104 96-97 0.4555068668832405 1104 98-99 -0.14413833964582423 1104 100-101 0.2592231150547626 1105 1 -0.2555332586890735 1105 2 -0.08443037807798248 1105 3 -0.2630560483171962 1105 4 -0.4906094396876526 1105 5 -0.5103084721435565 1105 6 -0.29750241424196133 1105 7 -0.2005936551568439 1105 8 -0.36462859426817573 1105 9 -0.40872428412943407 1105 10-11 -0.028674714701274695 1105 12-13 -0.24224913075708088 1105 14-15 -0.2353607788151848 1105 16-17 -0.35886061921204515 1105 18-19 -0.18507075488673763 1105 20-21 -0.3452467579978844 1105 22-23 -0.2751083271569783 1105 24-25 -0.22472294554597738 1105 26-27 -0.2760923572814207 1105 28-29 -0.14478928482414943 1105 30-31 -0.24935016045653668 1105 32-33 -0.6300462684709558 1105 34-35 -0.571363832463696 1105 36-37 -0.7507564798686417 1105 38-39 -0.9715106722759472 1105 40-41 -1.1048155009268825 1105 42-43 -1.3438873372419238 1105 44-45 -1.0693047417242312 1105 46-47 -0.9308519107193547 1105 48-49 -1.095331660299614 1105 50-51 -1.1198915648047674 1105 52-53 -1.2813591008056662 1105 54-55 -0.9733656221943932 1105 56-57 -1.2079582360806072 1105 58-59 -1.0369829567401965 1105 60-61 -1.0342245632738738 1105 62-63 -0.7728190691525327 1105 64-65 -0.8272469038107744 1105 66-67 -0.5807383042540337 1105 68-69 -0.8836308426641608 1105 70-71 -0.4006549825003347 1105 72-73 -0.7043287581809139 1105 74-75 -0.5714372886208352 1105 76-77 -0.6208017916321324 1105 78-79 -1.0793368461572825 1105 80-81 -1.0709116259336717 1105 82-83 -0.6984184359845891 1105 84-85 -0.6752033438497911 1105 86-87 -1.0110869378981313 1105 88-89 -0.6808396789203606 1105 90-91 -0.574576595342343 1105 92-93 -1.2824260222384538 1105 94-95 -0.9455831302012321 1105 96-97 -0.2586970641644015 1105 98-99 -0.29385845923109244 1105 100-101 -0.5075220029402807 1106 1 -0.25282078129304963 1106 2 -0.026944892211417937 1106 3 -0.2645788426447879 1106 4 0.04265409890566474 1106 5 -0.16625212875343465 1106 6 -0.49151592904161845 1106 7 -0.636779055366226 1106 8 -0.19502737603271214 1106 9 -0.10392748199752333 1106 10-11 -0.49146142832076833 1106 12-13 -0.6064349116650476 1106 14-15 -0.737169097079196 1106 16-17 -0.41791648672893444 1106 18-19 -0.6728170192819007 1106 20-21 -0.3946186053377474 1106 22-23 -0.11497698614622465 1106 24-25 -0.04224935651009787 1106 26-27 -0.3283194439855279 1106 28-29 -0.17605415586250217 1106 30-31 -0.17892092280545313 1106 32-33 -0.5143138995740273 1106 34-35 -0.4146825723513956 1106 36-37 0.05675279965929292 1106 38-39 -0.05117185053806139 1106 40-41 -0.2287090956732456 1106 42-43 -0.03345143736564893 1106 44-45 0.10281860461630288 1106 46-47 0.4592451674190343 1106 48-49 0.4089274050853575 1106 50-51 0.4479679574185127 1106 52-53 0.6399552610483354 1106 54-55 0.2681400406970127 1106 56-57 -0.3948336524696785 1106 58-59 0.5657818667108359 1106 60-61 0.29198653408978714 1106 62-63 -0.0917017188146616 1106 64-65 0.12259605802419316 1106 66-67 0.5210271854195128 1106 68-69 -0.6883562638119685 1106 70-71 -0.30291062159808035 1106 72-73 0.3234860319589856 1106 74-75 -0.03838789297983425 1106 76-77 0.3406524769507193 1106 78-79 0.16671340605552842 1106 80-81 0.30548030325639175 1106 82-83 0.46785166965926805 1106 84-85 0.204614872577352 1106 86-87 0.28276478000422145 1106 88-89 -0.47290687222006156 1106 90-91 0.17161641883868128 1106 92-93 0.25835631334734543 1106 94-95 -0.3761532263276237 1106 96-97 0.41842295106354044 1106 98-99 -0.2311145742020635 1106 100-101 -0.8649503840195578 1107 1 -0.053359864851003636 1107 2 -0.2370456157182943 1107 3 -0.09420006551327731 1107 4 -0.01612492412360922 1107 5 -0.4627007975953532 1107 6 -0.031639823683882184 1107 7 -0.11103072226933364 1107 8 -0.03495738974623919 1107 9 -0.4289854098359882 1107 10-11 0.05284745710033434 1107 12-13 -0.08943486437701864 1107 14-15 -0.33555184640534463 1107 16-17 -0.16556390716682046 1107 18-19 -0.3169272949711228 1107 20-21 -0.01655079430071993 1107 22-23 -0.11883096070526733 1107 24-25 -0.25372143292754856 1107 26-27 -0.3076782182797544 1107 28-29 -0.3931771520321732 1107 30-31 -0.05107772991757287 1107 32-33 0.04633300069551893 1107 34-35 0.4950918637388355 1107 36-37 -0.027963708592473324 1107 38-39 -0.05765031083975458 1107 40-41 -0.16406635675046033 1107 42-43 0.2254741785609511 1107 44-45 0.24543930731644537 1107 46-47 0.99823414930772 1107 48-49 0.7017585395252368 1107 50-51 0.628655525395061 1107 52-53 0.8842925192882731 1107 54-55 0.590880854996918 1107 56-57 0.7998038847696449 1107 58-59 1.1442327108021928 1107 60-61 1.2183827132168474 1107 62-63 1.3864039226474816 1107 64-65 1.2560665266485884 1107 66-67 1.037743504519149 1107 68-69 0.726690787779269 1107 70-71 1.0207286652984067 1107 72-73 1.3253066211678615 1107 74-75 1.2869537630470305 1107 76-77 0.9257709334693729 1107 78-79 1.0856380762215068 1107 80-81 0.9910858264984626 1107 82-83 0.8885969290341187 1107 84-85 1.1499499082798188 1107 86-87 0.8344269543079044 1107 88-89 1.1716999277988513 1107 90-91 0.9057394622963812 1107 92-93 1.107152832948234 1107 94-95 1.3408988377949491 1107 96-97 1.464055901363821 1107 98-99 1.0611491038610161 1107 100-101 1.5724875504392308 1108 1 -0.15251682992526838 1108 2 -0.16940209905832404 1108 3 0.02653457920771274 1108 4 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52-53 0.7539151607977033 2316 54-55 0.23515758455666713 2316 56-57 0.8895523124425964 2316 58-59 0.7396415158336467 2316 60-61 0.28033239875144744 2316 62-63 0.046969960383329123 2316 64-65 0.32958853922719555 2316 66-67 0.7712026240160057 2316 68-69 1.4475083978421637 2316 70-71 0.7160116841663324 2316 72-73 0.45436322494145287 2316 74-75 0.38273992656903744 2316 76-77 0.38426149950711164 2316 78-79 -0.03499085459610285 2316 80-81 0.39334997744185785 2316 82-83 0.5803579353234305 2316 84-85 -0.2191695885755287 2316 86-87 -0.007711410471969771 2316 88-89 0.21629112276740514 2316 90-91 0.4856765692146183 2316 92-93 0.07061195244509122 2316 94-95 0.4950497811911738 2316 96-97 0.38569112148960727 2316 98-99 -0.11432259425219016 2316 100-101 -0.09933634893183907 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 2.0 6 7.0 7 18.0 8 36.0 9 80.0 10 130.0 11 266.0 12 332.0 13 443.0 14 638.0 15 861.0 16 1194.0 17 1710.0 18 1427.0 19 952.0 20 832.0 21 767.0 22 802.0 23 928.0 24 1006.0 25 1124.0 26 1383.0 27 1774.0 28 2154.0 29 2793.0 30 3357.0 31 4320.0 32 5676.0 33 7308.0 34 10191.0 35 14812.0 36 23060.0 37 36113.0 38 31946.0 39 3030.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.29963317305309 20.50079313934467 16.558023100183412 21.641550587418827 2 15.011797637995205 21.213453642404613 40.79567481870034 22.97907390089984 3 18.81986462132988 25.29215410625925 31.175490639301927 24.712490633108942 4 12.620066512667751 18.212332711970422 38.4454366983954 30.72216407696643 5 12.90530371067741 37.88099323022799 35.133444407970124 14.080258651124474 6 30.241588377004202 37.97972416441139 17.702030680051774 14.076656778532634 7 26.923386572368134 33.77902187981891 21.277241396394444 18.020350151418505 8 25.90092461278356 35.39291398561989 20.098716194038634 18.60744520755792 9 25.529964761910662 17.21340409851802 21.164529054392993 36.09210208517833 10-11 24.251732487784334 27.45567370396289 27.68078873867458 20.611805069578196 12-13 25.548866965175975 24.666654280388187 28.365816348648963 21.41866240578687 14-15 22.266880531110463 26.260737089172803 26.90233042056567 24.570051959151066 16-17 21.027973716968162 29.368067726492974 27.965046788007903 21.63891176853096 18-19 20.926408749450374 27.304255200559847 31.008899320629457 20.760436729360325 20-21 21.417822174605043 26.283341487431333 31.57215138134549 20.726684956618136 22-23 21.11747966346794 26.325861380252118 31.619397970527125 20.937260985752815 24-25 21.177909551161964 26.50791930514484 31.461704005325984 20.852467138367214 26-27 20.95993063727008 26.86783922710101 31.291880844738962 20.88034929088995 28-29 20.83924269355348 26.959998018170893 31.29958443520967 20.901174853065953 30-31 21.005678968000844 26.76174964235507 31.090646733509626 21.14192465613446 32-33 20.949942095582543 26.622717110600536 31.047605482031056 21.379735311785872 34-35 21.230174704130103 26.629839044298425 31.282629294061547 20.857356957509925 36-37 21.51690994779313 26.228533562886675 31.216983644324436 21.037572844995758 38-39 21.065441281204908 26.914716392214178 30.92002997405139 21.099812352529526 40-41 21.262068581125018 26.99801205155041 30.956568590414495 20.783350776910073 42-43 21.09162083147515 26.76062880927605 30.70555225211833 21.44219810713047 44-45 21.13201587881565 26.964260278808222 30.776352702928662 21.12737113944746 46-47 21.551281019117745 27.2135279582345 30.11679971264546 21.11839131000229 48-49 21.517529246375556 27.610188700278066 29.615167860880764 21.25711419246561 50-51 21.488422213001552 27.496237761111765 29.84306973921337 21.172270286673314 52-53 21.737070593845413 27.374235940373932 29.535897642330294 21.35279582345036 54-55 21.39428882847287 27.508314083469067 29.827896923943943 21.269500164114124 56-57 21.51226520842494 27.58479745839862 29.69196088510153 21.210976448074913 58-59 21.368278288011 27.478587751512634 29.708681946827024 21.44445201364934 60-61 21.201377320047314 27.529989533853954 30.011518953633114 21.25711419246561 62-63 21.284053680801122 27.78823704272541 29.848643426455197 21.07906585001827 64-65 21.13820886463991 27.62752906058598 29.59565995553436 21.63860211923975 66-67 21.210047500201274 27.71546945929041 29.533730097291805 21.54075294321651 68-69 21.546945929040767 27.531847429601235 29.434951973394934 21.486254667963063 70-71 21.499569587485215 27.66468697553151 29.417921262378233 21.417822174605043 72-73 21.393934514556793 27.483263248509022 29.357903276749386 21.7648989601848 74-75 21.36053705573068 27.59966062437683 29.215410625925074 21.824391693967414 76-77 21.23302729566954 27.46938333152704 29.56602517456533 21.73156419823809 78-79 21.191468542728504 27.690697515993385 29.330290512965018 21.787543428313093 80-81 21.555306459903516 27.870603754188007 28.989366643339753 21.584723142568727 82-83 21.535555589961138 27.751474709315826 29.31551812228088 21.39745157844215 84-85 21.28407356142453 27.898904134191692 29.20130921747316 21.615713086910613 86-87 21.24844401231166 27.78730809485177 29.051915800164736 21.91233209267184 88-89 21.14378255188174 27.779566862571453 28.98905699404854 22.08759359149827 90-91 21.075336081235502 27.68144980226872 29.23480937590001 22.008404740595765 92-93 21.126882679349983 27.287083828775266 29.36298553309552 22.223047958779233 94-95 20.99638948926446 27.39622104005004 29.443002854966466 22.164386615719035 96-97 21.089903575210716 27.28784378812557 29.390981774042718 22.231270862620995 98-99 20.90380435119184 27.2692648306528 29.12747022722065 22.69946059093471 100-101 20.599611655296073 27.302738542437655 29.301753744863102 22.79589605740317 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 27.0 1 18.0 2 23.5 3 37.5 4 27.5 5 23.5 6 62.0 7 139.0 8 236.0 9 289.5 10 245.5 11 190.5 12 189.0 13 199.5 14 216.5 15 243.5 16 247.5 17 251.0 18 294.0 19 327.5 20 356.5 21 422.0 22 475.5 23 573.0 24 716.5 25 891.5 26 1148.0 27 1414.0 28 1626.0 29 1992.5 30 2353.5 31 2713.5 32 3215.0 33 3567.5 34 3933.5 35 4320.5 36 4749.0 37 5114.5 38 5365.0 39 5527.0 40 5533.0 41 5658.0 42 5751.0 43 5851.0 44 5998.0 45 6117.0 46 6117.0 47 6197.0 48 6311.5 49 6011.5 50 5519.0 51 5146.5 52 4875.0 53 4626.0 54 4429.0 55 4066.0 56 3539.0 57 3089.5 58 2786.5 59 2393.0 60 1915.5 61 1522.0 62 1256.5 63 917.5 64 545.0 65 404.5 66 315.0 67 236.0 68 174.5 69 124.5 70 91.0 71 60.5 72 41.5 73 33.5 74 27.0 75 16.5 76 13.0 77 7.5 78 3.0 79 1.5 80 1.0 81 0.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05511757383587349 2 0.0 3 0.0 4 0.0 5 0.012385971648510897 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0024771943297021795 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001548246456063862 24-25 3.0964929121277243E-4 26-27 0.0018578957472766345 28-29 0.003715791494553269 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.015482464560638622 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0012385971648510897 74-75 0.0 76-77 3.0964929121277243E-4 78-79 0.0 80-81 0.0 82-83 3.0964929121277243E-4 84-85 0.001548246456063862 86-87 0.0 88-89 0.0 90-91 0.010218426610021489 92-93 6.192985824255449E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.01145702377487258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 161473.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.11577081854915 #Duplication Level Percentage of deduplicated Percentage of total 1 89.34184157574215 69.79006821037801 2 6.96461927682029 10.880932065330867 3 1.6651804207291467 3.9023055635163986 4 0.7108465907232989 2.221133174723529 5 0.3685254326617171 1.4393824119304681 6 0.21193715414550518 0.9933380496699485 7 0.15915863420080928 0.8702959575116601 8 0.09747761622544329 0.6091631303204174 9 0.06365761921849535 0.4475397594353807 >10 0.38791102498255803 5.330814695410844 >50 0.017903578686565343 0.9067277824333537 >100 0.00795714608291793 0.8733179326950212 >500 0.0029839297810942238 1.7349812666441125 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 943 0.5839985632272887 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 777 0.4811949985446483 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 513 0.3177001727843045 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 208 0.12881410514451333 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 164 0.10156496751778936 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.192985824255449E-4 0.0 22-23 0.0 0.0 0.0 6.192985824255449E-4 0.0 24-25 0.0 0.0 0.0 0.0012385971648510897 0.0 26-27 0.0 0.0 0.0 0.0018578957472766345 0.0 28-29 0.0 0.0 0.0 0.003096492912127724 0.0 30-31 0.0 0.0 0.0 0.009289478736383174 0.0 32-33 0.0 0.0 0.0 0.01579211385185139 0.0 34-35 0.0 0.0 0.0 0.020746502511255753 6.192985824255449E-4 36-37 0.0 0.0 0.0 0.023842995423383476 6.192985824255449E-4 38-39 0.0 0.0 0.0 0.027868436209149517 6.192985824255449E-4 40-41 0.0 0.0 0.0 0.03777721352795824 6.192985824255449E-4 42-43 0.0 0.0 0.0 0.052021080923745765 6.192985824255449E-4 44-45 0.0 0.0 0.0 0.0752447777647037 6.192985824255449E-4 46-47 0.0 0.0 0.0 0.10125531822657657 6.192985824255449E-4 48-49 0.0 0.0 0.0 0.16690096796368434 6.192985824255449E-4 50-51 0.0 0.0 0.0 0.2248053854204728 6.192985824255449E-4 52-53 0.0 0.0 0.0 0.28085190712998453 6.192985824255449E-4 54-55 0.0 0.0 0.0 0.37870108315322065 6.192985824255449E-4 56-57 0.0 0.0 0.0 0.4641642875279458 6.192985824255449E-4 58-59 0.0 0.0 0.0 0.5480792454466072 6.192985824255449E-4 60-61 0.0 0.0 0.0 0.6607915874480563 6.192985824255449E-4 62-63 0.0 0.0 0.0 0.8023013135322934 6.192985824255449E-4 64-65 0.0 0.0 0.0 0.8914803094015717 6.192985824255449E-4 66-67 0.0 0.0 0.0 1.016268973760319 6.192985824255449E-4 68-69 0.0 0.0 0.0 1.206703287856174 6.192985824255449E-4 70-71 0.0 0.0 0.0 1.3649340756659007 6.192985824255449E-4 72-73 0.0 0.0 0.0 1.6049122763557995 6.192985824255449E-4 74-75 0.0 0.0 0.0 1.9368563165358914 6.192985824255449E-4 76-77 0.0 0.0 0.0 2.163209948412428 6.192985824255449E-4 78-79 0.0 0.0 0.0 2.39575656611322 6.192985824255449E-4 80-81 0.0 0.0 0.0 2.7047865587435673 6.192985824255449E-4 82-83 0.0 0.0 0.0 3.067385878753724 6.192985824255449E-4 84-85 0.0 0.0 0.0 3.455995739225753 6.192985824255449E-4 86-87 0.0 0.0 0.0 3.9068451072315495 6.192985824255449E-4 88-89 0.0 0.0 0.0 4.448421717562688 6.192985824255449E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 460 0.0 51.65856 1 GTATCAA 790 0.0 42.713127 1 CTATAGG 50 0.0016073954 38.0207 1 TAGGACA 80 1.0724374E-5 35.62233 4 TATAAGG 70 2.0464529E-4 33.926025 2 ATCAACG 1020 0.0 32.595592 3 TATCAAC 1030 0.0 32.279133 2 CAACGCA 1040 0.0 31.968756 5 TCAACGC 1040 0.0 31.968756 4 AACGCAG 1075 0.0 30.927914 6 CAGGGGT 80 4.478705E-4 29.685274 4 GTAAGGT 65 0.005831536 29.228577 4 GGTCTAC 100 4.9088652E-5 28.515526 1 TGGTATC 220 1.8189894E-12 28.066076 2 ACGCAGA 1175 0.0 27.891527 7 CGCAGAG 1170 0.0 27.604767 8 TCCCTAT 70 0.008348375 27.157642 1 GTGTGCA 170 1.11118425E-7 25.145174 9 GTGGTAT 290 0.0 24.58235 1 GCAGAGT 1295 0.0 24.573448 9 >>END_MODULE