FastQCFastQC Report
Thu 26 May 2016
SRR937224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202696
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.40257331175750877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5860.2891028930023286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.17908592177448002No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2880.14208469826735604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCG250.00501520857.0002945
GGTATCA6250.036.4892121
GTATCAA10250.034.3013421
ATATAGG753.0650067E-431.6746641
GTGTAGT902.3990697E-531.6746621
AGAGCGT300.00394785231.66683290-91
TAGTACT753.0694573E-431.6668324
CTGGACT804.4805647E-429.6876564
TATCAAC12050.029.564472
ATCAACG12450.027.8515513
CAACGCA12550.027.6296255
TCAACGC12550.027.6296254
AACGCAG12950.026.7762036
GGTAAGG908.9217024E-426.3890253
GTAAGGT908.9217024E-426.3890254
GTATTAT1109.4226314E-525.915631
TAGGCTG950.00122302525.000135
ACGCAGA13900.024.6044457
CGCAGAG13950.024.5162588
TAAGGTG1000.001648904523.7501225
Produced by FastQC (version 0.11.5)