##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937224_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 202696 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.801964518293406 33.0 31.0 34.0 30.0 34.0 2 31.910299167225798 33.0 31.0 34.0 30.0 34.0 3 31.94516912025891 33.0 31.0 34.0 30.0 34.0 4 35.54271421241663 37.0 35.0 37.0 33.0 37.0 5 35.56985830998145 37.0 35.0 37.0 33.0 37.0 6 35.59166929786478 37.0 35.0 37.0 33.0 37.0 7 35.493226309349964 37.0 35.0 37.0 33.0 37.0 8 35.49968918972254 37.0 35.0 37.0 33.0 37.0 9 37.129933496467615 39.0 37.0 39.0 34.0 39.0 10-11 37.02294075857442 39.0 37.0 39.0 33.0 39.0 12-13 36.895688617436946 39.0 37.0 39.0 33.0 39.0 14-15 38.09040632276907 40.0 38.0 41.0 33.0 41.0 16-17 38.010269072897344 40.0 38.0 41.0 33.0 41.0 18-19 37.997493783794454 40.0 38.0 41.0 33.0 41.0 20-21 37.871904230966564 40.0 38.0 41.0 33.0 41.0 22-23 37.726062675139126 40.0 37.5 41.0 32.5 41.0 24-25 37.67150560445199 40.0 37.5 41.0 32.5 41.0 26-27 37.69269250503217 40.0 37.5 41.0 32.5 41.0 28-29 37.614254844693534 40.0 37.0 41.0 32.0 41.0 30-31 37.40669278130797 40.0 37.0 41.0 32.0 41.0 32-33 37.16819769507045 39.5 36.5 41.0 31.5 41.0 34-35 37.202490429016855 40.0 37.0 41.0 31.0 41.0 36-37 37.08900520977227 40.0 37.0 41.0 31.0 41.0 38-39 36.98021421241663 40.0 36.5 41.0 31.0 41.0 40-41 36.771334905474205 39.5 36.0 41.0 30.5 41.0 42-43 36.51357944902711 39.0 36.0 41.0 30.0 41.0 44-45 36.29751203773138 39.0 36.0 41.0 30.0 41.0 46-47 35.96752525950191 39.0 35.0 41.0 28.5 41.0 48-49 35.672334431858545 39.0 35.0 41.0 27.5 41.0 50-51 34.72621068003315 38.0 34.0 39.5 25.5 40.5 52-53 34.840670264830095 38.0 34.5 39.5 26.5 40.5 54-55 35.21584540395469 39.0 35.0 40.0 26.5 41.0 56-57 35.00838201049848 38.5 35.0 40.0 26.0 41.0 58-59 34.76335004144137 38.0 34.0 40.0 25.5 41.0 60-61 34.4756161937088 38.0 34.0 40.0 24.5 41.0 62-63 34.173363065872046 38.0 34.0 40.0 23.5 41.0 64-65 33.76707483127442 37.0 33.5 40.0 22.5 41.0 66-67 33.42131319808975 37.0 33.0 39.5 22.0 41.0 68-69 32.95419248529818 36.0 32.5 39.0 20.5 41.0 70-71 32.49795506571417 35.5 32.0 39.0 18.5 40.0 72-73 32.03624393179935 35.0 31.5 38.0 17.5 40.0 74-75 31.554342463590796 35.0 31.0 37.5 14.0 39.5 76-77 31.06718928839247 35.0 31.0 37.0 9.5 39.0 78-79 30.549381832892607 34.5 30.0 36.0 7.0 39.0 80-81 30.075852508189605 34.0 30.0 36.0 4.0 37.5 82-83 29.58800864348581 34.0 29.0 35.5 2.0 37.0 84-85 29.03339730433753 34.0 29.0 35.0 2.0 37.0 86-87 28.59408177763745 34.0 29.0 35.0 2.0 36.0 88-89 28.10914620910131 34.0 27.5 35.0 2.0 36.0 90-91 27.600411453605396 33.0 26.5 35.0 2.0 35.5 92-93 27.10010064332794 33.0 26.0 35.0 2.0 35.0 94-95 26.555358763863126 33.0 25.0 35.0 2.0 35.0 96-97 25.819799108023837 32.0 23.5 34.5 2.0 35.0 98-99 24.897893890357977 32.0 17.5 34.0 2.0 35.0 100-101 22.294352133243873 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3201607653902272 1101 2 -0.15624033503908663 1101 3 -0.31497308680146574 1101 4 -0.23340582541991495 1101 5 -0.4187171691421696 1101 6 -0.31946045618217767 1101 7 -0.3111821218544293 1101 8 -0.6502109268098835 1101 9 -0.7033761017191154 1101 10-11 -0.7877959975728146 1101 12-13 -0.36633486766373125 1101 14-15 -0.4788923723900922 1101 16-17 -0.8363755546813252 1101 18-19 -0.7900177825060624 1101 20-21 -0.5704599540679354 1101 22-23 -0.43771275601593373 1101 24-25 -0.4709744934177138 1101 26-27 -0.8480345975461248 1101 28-29 -0.7963943345125557 1101 30-31 -0.7189384729312707 1101 32-33 -1.0922467404807321 1101 34-35 -0.7732620547115658 1101 36-37 -0.47898445574784176 1101 38-39 -0.775320621242436 1101 40-41 -0.5950704423568141 1101 42-43 -0.9789457277433229 1101 44-45 -1.0303871909080797 1101 46-47 -0.7005763050166607 1101 48-49 -0.9644602182329365 1101 50-51 -0.7870124119999602 1101 52-53 -0.41874721939691284 1101 54-55 -0.9429923970084317 1101 56-57 -1.183959786405758 1101 58-59 -0.7993310671151619 1101 60-61 -0.6285038532464569 1101 62-63 -0.6174753649157125 1101 64-65 -0.6165399980621444 1101 66-67 -1.044097980189008 1101 68-69 -1.3692176334118962 1101 70-71 -1.5220810372492437 1101 72-73 -0.8979486001685686 1101 74-75 -0.9018816340625229 1101 76-77 -0.6529919915242921 1101 78-79 -1.2654307711063986 1101 80-81 -1.188592197728962 1101 82-83 -0.7099693446283588 1101 84-85 -0.9798408796412019 1101 86-87 -0.9641183152708841 1101 88-89 -1.1166071615695827 1101 90-91 -1.4929701435991056 1101 92-93 -1.3611281816288248 1101 94-95 -0.9357007170488636 1101 96-97 -1.0827451389314895 1101 98-99 -1.3806357280749708 1101 100-101 -1.0422436273399889 1102 1 -0.18202808439540874 1102 2 -0.28868835181350505 1102 3 -0.09212958980105768 1102 4 -0.1115816073524627 1102 5 0.12694070260341306 1102 6 -0.03329352741521774 1102 7 -0.05979881473112414 1102 8 -0.04656482045540855 1102 9 -0.05061822414907624 1102 10-11 -0.1425691352045959 1102 12-13 -0.1258957040549049 1102 14-15 -0.3008854995378627 1102 16-17 -0.11752180664706913 1102 18-19 -0.24978829913487033 1102 20-21 -0.1487580494724412 1102 22-23 -0.09702314101215137 1102 24-25 -0.4006733692986657 1102 26-27 -0.4511040832470954 1102 28-29 -0.38653353889255015 1102 30-31 -0.420697457147007 1102 32-33 -0.8039014670227544 1102 34-35 -0.4956978401387886 1102 36-37 -0.5845060353389684 1102 38-39 -0.3470079647103148 1102 40-41 -0.4023160594574051 1102 42-43 -0.4768955209753045 1102 44-45 -0.3261819372532315 1102 46-47 -0.5420871676178578 1102 48-49 -0.9232988226944627 1102 50-51 -1.1002106180690348 1102 52-53 -1.4694315923947627 1102 54-55 -1.3155009880737296 1102 56-57 -1.161786101275574 1102 58-59 -1.3820790431184236 1102 60-61 -1.0600316067556932 1102 62-63 -1.3549265851382017 1102 64-65 -0.9124978290365746 1102 66-67 -1.3023599639970271 1102 68-69 -1.4871802404361816 1102 70-71 -1.4282433064553715 1102 72-73 -1.298204876014573 1102 74-75 -1.1955155090698035 1102 76-77 -1.437533898449562 1102 78-79 -1.6031499160769833 1102 80-81 -1.4313378439518054 1102 82-83 -1.365295805109458 1102 84-85 -1.2448941733682695 1102 86-87 -1.130790806388596 1102 88-89 -0.9907698967317984 1102 90-91 -1.1569226160785782 1102 92-93 -1.1469747655882863 1102 94-95 -1.025537049974485 1102 96-97 -0.9903984676052637 1102 98-99 -0.5144058952367132 1102 100-101 -0.06615882333276701 1103 1 -0.30681330776310745 1103 2 -0.16127211470010394 1103 3 -0.12058749358112664 1103 4 0.017163560173308667 1103 5 -0.05282733863369771 1103 6 -0.5443545239787895 1103 7 -0.5211927150747684 1103 8 -0.14359016409802194 1103 9 -0.1680741949394573 1103 10-11 -0.18312835986095166 1103 12-13 -0.1727371452061064 1103 14-15 -0.42889237239009503 1103 16-17 0.0584205258271524 1103 18-19 -0.5194470727603004 1103 20-21 -0.21411726338997283 1103 22-23 -0.23850724754135655 1103 24-25 -0.4174125230787311 1103 26-27 -0.8087138878003586 1103 28-29 -0.40868909086849214 1103 30-31 -0.18745674623635722 1103 32-33 -0.06350600954853292 1103 34-35 -0.020746164881060736 1103 36-37 0.3031792095063892 1103 38-39 -0.1987382589543003 1103 40-41 -0.01638929828902036 1103 42-43 0.12190172988380255 1103 44-45 0.22696185570209337 1103 46-47 -0.2580048007793678 1103 48-49 -0.34371736865666236 1103 50-51 -0.21863979547453738 1103 52-53 -0.30177158380369207 1103 54-55 -0.19455489700843742 1103 56-57 -0.2092047546260929 1103 58-59 -0.07043143787787187 1103 60-61 0.05810896455015069 1103 62-63 -0.3819615937292724 1103 64-65 -0.4176390446723133 1103 66-67 -0.5758511581551033 1103 68-69 -0.0745473473949474 1103 70-71 0.12973967249651963 1103 72-73 -0.13477328237195962 1103 74-75 -0.1923609772828634 1103 76-77 0.0923536122892692 1103 78-79 0.0966057755037717 1103 80-81 -0.028846435017097605 1103 82-83 -0.22051621962836165 1103 84-85 0.04302061400286661 1103 86-87 -0.44221021145732564 1103 88-89 -0.5837681785187385 1103 90-91 -0.30566214571775063 1103 92-93 -0.4427423235779777 1103 94-95 -0.4118262467098823 1103 96-97 -0.3244029779145414 1103 98-99 -0.3636998170580199 1103 100-101 0.3167275061345869 1104 1 0.007134568684769249 1104 2 0.060433966380976756 1104 3 -0.12722359396722638 1104 4 -0.1212142149232136 1104 5 -0.27555899886535684 1104 6 -0.008760894635159389 1104 7 -0.12441424017129066 1104 8 -0.11758871622157585 1104 9 0.20617170274393715 1104 10-11 0.17108103927032658 1104 12-13 -0.03926857619065771 1104 14-15 -0.23680743030514861 1104 16-17 -0.07507066625779402 1104 18-19 -0.31565092169851994 1104 20-21 -0.38923490335136535 1104 22-23 -0.22433012398923324 1104 24-25 -0.39136582906328243 1104 26-27 -0.02926619233704031 1104 28-29 0.21490073779946073 1104 30-31 -0.0892349194989066 1104 32-33 -0.0027630679462191665 1104 34-35 0.18403786021546864 1104 36-37 0.0949112317071652 1104 38-39 0.10402628013836335 1104 40-41 -0.17683210716932507 1104 42-43 -0.012268154286076083 1104 44-45 -0.013732521711027346 1104 46-47 -0.1404100855284014 1104 48-49 -0.490322400027317 1104 50-51 -0.5834066270382436 1104 52-53 -0.6436212459658535 1104 54-55 -1.0128765668926079 1104 56-57 -0.7280799356106513 1104 58-59 -0.4179293263913877 1104 60-61 -0.18901036460042775 1104 62-63 -0.4490766999068825 1104 64-65 -0.5397583738228917 1104 66-67 -0.7386467662632157 1104 68-69 -0.38830669826367625 1104 70-71 -0.33960606833359464 1104 72-73 -0.5862543393217692 1104 74-75 -0.6046890419546784 1104 76-77 -0.6152130503555178 1104 78-79 -0.41402706716032256 1104 80-81 -0.7938078249784866 1104 82-83 -0.753598688257707 1104 84-85 -0.7797427185260908 1104 86-87 -0.7827100304727672 1104 88-89 -0.6626774449280077 1104 90-91 -0.7782656085555892 1104 92-93 -0.2902100479988263 1104 94-95 -0.5217291183315034 1104 96-97 -0.4076257095747735 1104 98-99 -0.27192556512751764 1104 100-101 0.07886101240871923 1105 1 0.138849957543016 1105 2 0.21200594652438554 1105 3 0.009897200296421715 1105 4 0.30184149384677994 1105 5 0.30304000830507505 1105 6 0.05985465969467896 1105 7 0.17619248900686557 1105 8 -0.07566924573067269 1105 9 0.31869238669319344 1105 10-11 0.09694976513905118 1105 12-13 0.33508332673266494 1105 14-15 0.08029130107929205 1105 16-17 0.1772183574598074 1105 18-19 0.35318526142337703 1105 20-21 0.2231754662018659 1105 22-23 0.4072197932749688 1105 24-25 0.3809452575335186 1105 26-27 0.32169905862821224 1105 28-29 0.38164094739681786 1105 30-31 0.35290517978405234 1105 32-33 0.3071652276963661 1105 34-35 0.4262882738944498 1105 36-37 -0.05892219355483519 1105 38-39 0.5746274935967222 1105 40-41 0.5504155486497595 1105 42-43 0.5045547911082906 1105 44-45 0.760619381212301 1105 46-47 0.7616858879961441 1105 48-49 1.041558460424973 1105 50-51 0.39050401830097314 1105 52-53 0.2920103039514075 1105 54-55 0.34476270503237316 1105 56-57 0.36668013057798987 1105 58-59 0.7710943912037607 1105 60-61 1.0921810308766808 1105 62-63 0.7142437634135845 1105 64-65 0.5598003685929882 1105 66-67 0.2592572602122374 1105 68-69 -0.15835633974188568 1105 70-71 0.37622404749652105 1105 72-73 0.2492668961994653 1105 74-75 0.09773787475795359 1105 76-77 0.2642684719831472 1105 78-79 -0.18462828061867853 1105 80-81 -0.060989292159955966 1105 82-83 -0.16507138544468702 1105 84-85 0.09411276961511206 1105 86-87 -0.11216397421242874 1105 88-89 0.3649563112771794 1105 90-91 0.4350824333638812 1105 92-93 0.4469659034628357 1105 94-95 0.5692802583921583 1105 96-97 0.6179120731058667 1105 98-99 1.3138990349827964 1105 100-101 1.0761583097060132 1106 1 -0.27109902204882275 1106 2 0.0016743138713266603 1106 3 0.062448220704588664 1106 4 0.22977516731616277 1106 5 0.11235123279487169 1106 6 -0.039890238264504774 1106 7 -0.2644962865033378 1106 8 -0.19046516409802194 1106 9 -0.4504402663680267 1106 10-11 -0.43787166343238226 1106 12-13 -0.12390902020610639 1106 14-15 -0.38424951524723383 1106 16-17 -0.0347714384585629 1106 18-19 -0.24796269776030044 1106 20-21 -0.39882708481854223 1106 22-23 -0.19163224754135655 1106 24-25 -0.1679705587930158 1106 26-27 -0.2738366556574974 1106 28-29 -0.29233864443992275 1106 30-31 -0.4159723712363572 1106 32-33 0.16054532973718239 1106 34-35 0.43795919226180047 1106 36-37 -0.023271683350749583 1106 38-39 -0.2877449553828697 1106 40-41 0.004816058853840843 1106 42-43 0.005830301312371944 1106 44-45 -0.16533725144076783 1106 46-47 -0.47173247935079843 1106 48-49 0.023191113486198844 1106 50-51 -0.5464857776173986 1106 52-53 -0.16226265523226147 1106 54-55 -0.3876352541512986 1106 56-57 -0.2100418081975235 1106 58-59 -0.020487241449302473 1106 60-61 -0.4692347854498493 1106 62-63 -0.2335240937292724 1106 64-65 0.07510649104197142 1106 66-67 -0.22428865815510335 1106 68-69 0.016133456176483207 1106 70-71 0.06193833321080433 1106 72-73 0.16935618191375568 1106 74-75 0.343911344145706 1106 76-77 0.31528888014641154 1106 78-79 0.1808691683609176 1106 80-81 0.07829642212575649 1106 82-83 0.36458422680020774 1106 84-85 -0.07221376099713339 1106 86-87 0.10326969925695906 1106 88-89 0.21087467862411913 1106 90-91 0.8609115149965341 1106 92-93 0.594925087136307 1106 94-95 0.3030175032901177 1106 96-97 0.17001666494260093 1106 98-99 1.2975725043705495 1106 100-101 1.6127654525631563 1107 1 0.01807407962428087 1107 2 0.07845198439899548 1107 3 -0.06287352961716408 1107 4 0.192170598461594 1107 5 -0.09280481611117608 1107 6 -0.18765407352833563 1107 7 0.15293453717748662 1107 8 0.020120421487561657 1107 9 0.18236217893441875 1107 10-11 0.48478760297688694 1107 12-13 0.2734727365506515 1107 14-15 0.3413778978801787 1107 16-17 0.31070796952084123 1107 18-19 0.24162063557303526 1107 20-21 0.16063639651994066 1107 22-23 0.2692348695757616 1107 24-25 -0.08538127307873111 1107 26-27 -0.17180158487243347 1107 28-29 -0.16219063929192146 1107 30-31 0.3103789801149972 1107 32-33 -0.0558518710350171 1107 34-35 0.1814638576414609 1107 36-37 0.11325100004692956 1107 38-39 0.1156092917213769 1107 40-41 -0.34768152801874663 1107 42-43 -0.11458475660268164 1107 44-45 0.16323015525164664 1107 46-47 0.0790236339053152 1107 48-49 0.030913121208200778 1107 50-51 0.00378771015609658 1107 52-53 -0.15263025497485927 1107 54-55 0.13223282821678595 1107 56-57 -0.04146474899545893 1107 58-59 -0.05531671377877245 1107 60-61 0.17392399833393313 1107 62-63 0.3102540594238832 1107 64-65 0.5911940071294879 1107 66-67 0.11720908959264875 1107 68-69 -0.29306860302558135 1107 70-71 -0.007559736287262808 1107 72-73 0.5675423144748848 1107 74-75 0.4261989889333542 1107 76-77 0.29405335891089024 1107 78-79 0.2664748633416103 1107 80-81 -0.11555814672881226 1107 82-83 -0.1892486239076412 1107 84-85 -0.12852006730343746 1107 86-87 0.02166577390303459 1107 88-89 -0.2656375478881081 1107 90-91 0.07308574279576163 1107 92-93 0.5817333239445439 1107 94-95 0.5706132240108381 1107 96-97 0.3307912788422165 1107 98-99 -0.05313534633729944 1107 100-101 0.127767061314767 1108 1 0.3466491335514448 1108 2 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1.503436535267518 2316 58-59 0.7764352509519199 2316 60-61 1.1986508528480257 2316 62-63 1.343271119036686 2316 64-65 1.5287539074553465 2316 66-67 1.61414219290873 2316 68-69 1.8269237297327123 2316 70-71 1.9677134091986446 2316 72-73 1.2792061059259154 2316 74-75 1.3270040493128867 2316 76-77 1.4596657798424637 2316 78-79 1.5031051106101536 2316 80-81 0.8142386713658816 2316 82-83 0.962371080903555 2316 84-85 1.3799138985773318 2316 86-87 1.3527947752447993 2316 88-89 2.1462850129706226 2316 90-91 1.7510615909843779 2316 92-93 0.8092108014220223 2316 94-95 1.8711690990347982 2316 96-97 2.004885585915247 2316 98-99 1.609122656346237 2316 100-101 1.9050336896452258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 889.0 3 204.0 4 182.0 5 146.0 6 190.0 7 204.0 8 268.0 9 302.0 10 439.0 11 494.0 12 688.0 13 862.0 14 1041.0 15 1247.0 16 1518.0 17 2000.0 18 1724.0 19 1286.0 20 1246.0 21 1279.0 22 1392.0 23 1576.0 24 1764.0 25 1992.0 26 2349.0 27 2669.0 28 3231.0 29 3884.0 30 4776.0 31 5959.0 32 7606.0 33 9933.0 34 13888.0 35 19480.0 36 28555.0 37 40295.0 38 33114.0 39 4024.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94025275399007 20.032434784328462 15.790202321855903 22.237110139825564 2 14.664517946918053 20.428985965762195 41.42003351607338 23.48646257124637 3 18.584534472190917 25.2911402416118 30.331969076853117 25.79235620934417 4 12.270329855018018 17.439854474273485 38.76204269875087 31.527772971957628 5 13.077565709144656 37.81297425022122 35.04817269999456 14.061287340639566 6 30.381749590858973 37.558404572490886 18.12037398703604 13.939471849614096 7 27.21478932431497 33.510832913069706 21.27967802861748 17.994699733997845 8 25.682997973006376 35.469916448311665 20.149799772581204 18.697285806100755 9 25.457547682846037 17.718540197949316 21.141619783066535 35.68229233613811 10-11 24.142670674788384 27.505735305751127 27.81079424096195 20.540799778498535 12-13 25.19009996934669 24.831901197456762 28.219092068702967 21.758906764493577 14-15 22.1968172672398 26.318599558036176 26.560097686858253 24.92448548786577 16-17 21.275885681513106 29.61715024510592 27.4427159169285 21.66424815645248 18-19 21.107274134022834 28.1191369026284 29.88112512143332 20.892463841915447 20-21 21.912502255542453 26.913997711087877 30.341433511552086 20.832066521817584 22-23 21.60274267183101 26.812484396975545 30.239341125988396 21.345431805205045 24-25 21.344534907287944 27.193229622379423 30.327791866253406 21.13444360407923 26-27 21.28098713920947 27.28746820643509 30.088020901565393 21.34352375279005 28-29 21.187745809344648 27.559816182196172 29.95913786582487 21.293300142634312 30-31 21.276632561496836 27.43088944536045 29.93995545480838 21.352522538334327 32-33 21.013912372815994 27.579029594692138 29.72274134061088 21.68431669188099 34-35 21.631957835197326 27.473869490838254 29.73933766452085 21.15483500944357 36-37 21.63422085562026 27.1570944108836 29.74822775674046 21.460456976755683 38-39 21.18455754585699 27.606637121112875 29.767186638752463 21.44161869427767 40-41 21.476539926491853 27.505740506845356 29.70208685034641 21.315632716316387 42-43 21.318347764399963 27.598395321417712 29.393400845842592 21.68985606833973 44-45 21.49646377126867 27.6461150924413 29.495188207462046 21.36223292882799 46-47 21.818055964144182 27.826327720767647 28.906155363663107 21.449460951425063 48-49 21.50558103314009 27.996143339225455 28.78774768538548 21.710527942248977 50-51 21.62936556399119 28.125517613059053 28.643439694833884 21.601677128115877 52-53 21.778704211978695 28.191128980411357 28.599122689890162 21.431044117719786 54-55 21.50413227226767 28.246299743633486 28.804521148383554 21.445046835715292 56-57 21.536073479214092 28.315994235955085 28.43216495253067 21.715767332300157 58-59 21.677709868198296 27.979378334894456 28.581914522901563 21.760997274005682 60-61 21.44576526703408 28.143489113511112 28.81348392358451 21.5972616958703 62-63 21.373385466354925 28.263050968102306 28.924682283505437 21.43888128203734 64-65 21.237883873008656 28.19912709514465 28.75223045479035 21.810758577056344 66-67 21.293580044930046 28.328419366876815 28.510561526731898 21.867439061461237 68-69 21.547115624636998 28.132700297322614 28.631698100150516 21.688485977889872 70-71 21.524183781913447 28.06455599219001 28.5200563505598 21.891203875336743 72-73 21.556952827250754 28.082936643285432 28.40226595287174 21.957844576592077 74-75 21.536016530232928 28.475600110728834 28.247221892672126 21.741161466366115 76-77 21.51806746472154 28.25193354870763 28.330783281879146 21.89921570469168 78-79 21.478940252652706 28.289836302204467 28.349650384218965 21.881573060923856 80-81 21.644854731274485 28.380479244676778 28.10884959033107 21.86581643371767 82-83 21.75251973524861 28.380201974266324 28.248221726815714 21.619056563669346 84-85 21.56146967667311 28.503531830966423 28.24748026475139 21.687518227609083 86-87 21.540929471493474 28.389120408133 28.149114871445025 21.9208352489285 88-89 21.59268413247652 28.439940682155214 28.008403361344534 21.958971824023727 90-91 21.527688520213957 28.509387698604943 28.148512502100985 21.814411279080115 92-93 21.620966984513025 28.11729173154588 28.2828882001394 21.9788530838017 94-95 21.3398945622703 28.264528582621313 28.273426281330405 22.122150573777986 96-97 21.594953729336392 28.030332990587674 28.238452107885788 22.136261172190146 98-99 21.540967560138817 28.061567514657753 27.924382792339408 22.473082132864022 100-101 21.792910507702153 27.632609786297007 28.34956639077471 22.22491331522612 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 285.0 1 169.5 2 46.5 3 38.0 4 33.5 5 36.5 6 55.5 7 94.5 8 161.5 9 209.0 10 179.0 11 159.0 12 177.0 13 189.0 14 205.0 15 224.5 16 238.0 17 267.0 18 295.5 19 345.5 20 399.5 21 457.5 22 564.5 23 696.0 24 853.0 25 1041.5 26 1366.5 27 1775.5 28 2095.0 29 2406.5 30 2784.5 31 3315.5 32 3977.0 33 4470.5 34 4872.0 35 5348.0 36 5884.0 37 6423.0 38 6721.0 39 6884.5 40 7003.5 41 7156.0 42 7281.5 43 7344.5 44 7533.0 45 7765.0 46 7728.0 47 7623.0 48 7736.5 49 7499.5 50 6897.0 51 6518.0 52 6264.5 53 5938.5 54 5721.5 55 5279.0 56 4623.0 57 4066.0 58 3677.5 59 3168.5 60 2497.0 61 2012.0 62 1624.0 63 1185.0 64 797.0 65 576.0 66 421.5 67 301.0 68 242.0 69 192.5 70 129.5 71 93.5 72 58.5 73 41.5 74 35.5 75 18.5 76 12.5 77 7.5 78 5.0 79 5.5 80 4.5 81 3.0 82 1.0 83 1.5 84 1.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2190472431621739 2 0.19980660693846944 3 0.19141966294352133 4 0.1948731104708529 5 0.19881990764494614 6 0.21855389351541224 7 0.2180605438686506 8 0.21016694952046416 9 0.20819355093341751 10-11 0.2170738445751273 12-13 0.21411374669455738 14-15 0.20572680269960925 16-17 0.2153471208114615 18-19 0.20942692505032165 20-21 0.20548012787622844 22-23 0.20301337964242017 24-25 0.19881990764494614 26-27 0.20449342858270514 28-29 0.2123870229308916 30-31 0.21189367328412995 32-33 0.21214034810751076 34-35 0.2180605438686506 36-37 0.20227335517227768 38-39 0.20202668034889687 40-41 0.20005328176185025 42-43 0.20350672928918181 44-45 0.2133737222244149 46-47 0.21781386904526975 48-49 0.22126731657260132 50-51 0.21978726763231637 52-53 0.24026127797292496 54-55 0.22028061727907802 56-57 0.20103998105537357 58-59 0.18969293917985555 60-61 0.18796621541618977 62-63 0.1938864111773296 64-65 0.18895291470971307 66-67 0.1882128902395706 68-69 0.19265303706042547 70-71 0.19289971188380628 72-73 0.19585980976437622 74-75 0.19733985870466117 76-77 0.20350672928918181 78-79 0.1985732328215653 80-81 0.1965998342345187 82-83 0.1938864111773296 84-85 0.1938864111773296 86-87 0.20178000552551603 88-89 0.19536646011761458 90-91 0.20227335517227768 92-93 0.19585980976437622 94-95 0.1956131349409954 96-97 0.20128665587875438 98-99 0.2042467537593243 100-101 0.18870623988633226 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 202696.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.98900101963845 #Duplication Level Percentage of deduplicated Percentage of total 1 86.5552209861515 64.90689554785546 2 9.004110603761516 13.504185184928192 3 1.9785475935636156 4.451079225334354 4 0.841733359173755 2.524829749173776 5 0.44230907305681216 1.6584157765226308 6 0.2814760147813934 1.2664563095667407 7 0.17908533829876624 0.9400601431401935 8 0.1375384627894243 0.8251097541084514 9 0.08835678981839079 0.5963208661604958 >10 0.4562975224973176 5.846982672990475 >50 0.024530733408007932 1.2854671925508552 >100 0.008831064026882855 1.16258624168881 >500 0.001962458672640634 1.0316113359795642 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 816 0.40257331175750877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 586 0.2891028930023286 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 363 0.17908592177448002 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 288 0.14208469826735604 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 2.4667482338082647E-4 16-17 0.0 0.0 0.0 0.0 4.933496467616529E-4 18-19 0.0 0.0 0.0 0.0 4.933496467616529E-4 20-21 0.0 0.0 0.0 0.0 4.933496467616529E-4 22-23 0.0 0.0 0.0 4.933496467616529E-4 4.933496467616529E-4 24-25 0.0 0.0 0.0 7.400244701424794E-4 4.933496467616529E-4 26-27 0.0 0.0 0.0 0.0012333741169041324 4.933496467616529E-4 28-29 4.933496467616529E-4 0.0 0.0 0.0017267237636657852 4.933496467616529E-4 30-31 4.933496467616529E-4 0.0 0.0 0.0037001223507123968 4.933496467616529E-4 32-33 4.933496467616529E-4 0.0 0.0 0.007646919524805621 4.933496467616529E-4 34-35 4.933496467616529E-4 0.0 0.0 0.011593716698898844 4.933496467616529E-4 36-37 4.933496467616529E-4 0.0 0.0 0.014060464932707108 4.933496467616529E-4 38-39 4.933496467616529E-4 0.0 0.0 0.0214607096341319 4.933496467616529E-4 40-41 4.933496467616529E-4 0.0 0.0 0.027134230571890912 4.933496467616529E-4 42-43 7.400244701424794E-4 0.0 0.0 0.04218139479812133 4.933496467616529E-4 44-45 9.866992935233059E-4 0.0 0.0 0.06314875478549158 4.933496467616529E-4 46-47 9.866992935233059E-4 0.0 0.0 0.09200970912104828 4.933496467616529E-4 48-49 9.866992935233059E-4 0.0 0.0 0.1425780479141177 4.933496467616529E-4 50-51 9.866992935233059E-4 0.0 0.0 0.1956131349409954 4.933496467616529E-4 52-53 9.866992935233059E-4 0.0 0.0 0.2538283932588704 4.933496467616529E-4 54-55 9.866992935233059E-4 0.0 0.0 0.33029758850692664 4.933496467616529E-4 56-57 9.866992935233059E-4 0.0 0.0 0.4094802068121719 4.933496467616529E-4 58-59 9.866992935233059E-4 0.0 0.0 0.4783024825354225 4.933496467616529E-4 60-61 9.866992935233059E-4 0.0 0.0 0.5508248806093854 4.933496467616529E-4 62-63 9.866992935233059E-4 0.0 0.0 0.6689821210088014 4.933496467616529E-4 64-65 9.866992935233059E-4 0.0 0.0 0.7659253265974661 4.933496467616529E-4 66-67 9.866992935233059E-4 0.0 0.0 0.8821091684098354 4.933496467616529E-4 68-69 9.866992935233059E-4 0.0 0.0 1.041954453960611 4.933496467616529E-4 70-71 9.866992935233059E-4 0.0 0.0 1.1761455578797806 4.933496467616529E-4 72-73 9.866992935233059E-4 0.0 0.0 1.3638650984725895 4.933496467616529E-4 74-75 9.866992935233059E-4 0.0 0.0 1.6019063030350869 4.933496467616529E-4 76-77 9.866992935233059E-4 0.0 0.0 1.7851856968070412 4.933496467616529E-4 78-79 9.866992935233059E-4 0.0 0.0 1.991405849153412 4.933496467616529E-4 80-81 0.0019733985870466117 0.0 0.0 2.278288668745313 4.933496467616529E-4 82-83 0.0019733985870466117 0.0 0.0 2.589592295851916 9.866992935233059E-4 84-85 0.0019733985870466117 0.0 0.0 2.9132296641275603 9.866992935233059E-4 86-87 0.0019733985870466117 0.0 0.0 3.291875518017129 9.866992935233059E-4 88-89 0.0019733985870466117 0.0 0.0 3.7144294904684845 9.866992935233059E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCACCG 25 0.005015208 57.000294 5 GGTATCA 625 0.0 36.489212 1 GTATCAA 1025 0.0 34.301342 1 ATATAGG 75 3.0650067E-4 31.674664 1 GTGTAGT 90 2.3990697E-5 31.674662 1 AGAGCGT 30 0.003947852 31.666832 90-91 TAGTACT 75 3.0694573E-4 31.666832 4 CTGGACT 80 4.4805647E-4 29.687656 4 TATCAAC 1205 0.0 29.56447 2 ATCAACG 1245 0.0 27.851551 3 CAACGCA 1255 0.0 27.629625 5 TCAACGC 1255 0.0 27.629625 4 AACGCAG 1295 0.0 26.776203 6 GGTAAGG 90 8.9217024E-4 26.389025 3 GTAAGGT 90 8.9217024E-4 26.389025 4 GTATTAT 110 9.4226314E-5 25.91563 1 TAGGCTG 95 0.001223025 25.00013 5 ACGCAGA 1390 0.0 24.604445 7 CGCAGAG 1395 0.0 24.516258 8 TAAGGTG 100 0.0016489045 23.750122 5 >>END_MODULE